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Protein

Splicing factor 3B subunit 4

Gene

sap-49

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-CEL-72163. mRNA Splicing - Major Pathway.
R-CEL-72165. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor 3B subunit 4
Alternative name(s):
Spliceosome-associated protein 49
Gene namesi
Name:sap-49
ORF Names:C08B11.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiC08B11.5; CE36374; WBGene00004723; sap-49.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000820131 – 388Splicing factor 3B subunit 4Add BLAST388

Proteomic databases

EPDiQ09442.
PaxDbiQ09442.
PeptideAtlasiQ09442.
PRIDEiQ09442.

PTM databases

iPTMnetiQ09442.

Expressioni

Gene expression databases

BgeeiWBGene00004723.
ExpressionAtlasiQ09442. baseline.

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi606934. 1 interactor.
IntActiQ09442. 16 interactors.
STRINGi6239.C08B11.5.1.

Structurei

3D structure databases

ProteinModelPortaliQ09442.
SMRiQ09442.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 91RRM 1PROSITE-ProRule annotationAdd BLAST79
Domaini100 – 179RRM 2PROSITE-ProRule annotationAdd BLAST80

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi200 – 387Pro-richAdd BLAST188

Sequence similaritiesi

Belongs to the SF3B4 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0131. Eukaryota.
ENOG410XPT4. LUCA.
GeneTreeiENSGT00870000136537.
InParanoidiQ09442.
KOiK12831.
OMAiPNAWAPP.
OrthoDBiEOG091G0HAI.
PhylomeDBiQ09442.

Family and domain databases

CDDicd12334. RRM1_SF3B4. 1 hit.
cd12335. RRM2_SF3B4. 1 hit.
InterProiView protein in InterPro
IPR000504. RRM_dom.
IPR034158. SF3B4_RRM1.
IPR034159. SF3B4_RRM2.
PfamiView protein in Pfam
PF00076. RRM_1. 2 hits.
SMARTiView protein in SMART
SM00360. RRM. 2 hits.
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiView protein in PROSITE
PS50102. RRM. 2 hits.

Sequencei

Sequence statusi: Complete.

Q09442-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAGPIVERN QDATIYVGGL DEKVSESILW ELMVQAGPVV SVNMPKDRVT
60 70 80 90 100
ANHQGFGFVE FMGEEDADYA IKILNMIKLY GKPIKVNKAS AHEKNMDVGA
110 120 130 140 150
NIFVGNLDPE VDEKLLYDTF SAFGVILQVP KIMRDVDSGT SKGFAFINFA
160 170 180 190 200
SFEASDTALE AMNGQFLCNR AITVSYAFKR DSKGERHGTA AERMLAAQNP
210 220 230 240 250
LFPKDRPHQV FSDVPLGVPA NTPLAMPGVH AAIAAHATGR PGYQPPPLMG
260 270 280 290 300
MAQSGYQGQY PPVPPPPPSV TPMPPPMPPT PGMTPRPPPP PSSGMWPPPP
310 320 330 340 350
PPPPGRTPGP PGMPGMPPPP PPSRFGPPGM GGMPPPPPPG MRYPGGMPPP
360 370 380
PPPRYPSAGP GMYPPPPPSR PPAPPSGHGM IPPPPPPS
Length:388
Mass (Da):41,008
Last modified:November 23, 2004 - v2
Checksum:i0D6B0CF04CB24C3D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46676 Genomic DNA. Translation: CAB60993.2.
PIRiT19069.
RefSeqiNP_001021932.1. NM_001026761.2.
UniGeneiCel.30808.

Genome annotation databases

EnsemblMetazoaiC08B11.5; C08B11.5; WBGene00004723.
GeneIDi4363023.
KEGGicel:CELE_C08B11.5.
UCSCiC08B11.5.1. c. elegans.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSF3B4_CAEEL
AccessioniPrimary (citable) accession number: Q09442
Secondary accession number(s): Q8T8N6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 23, 2004
Last modified: June 7, 2017
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families