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Protein

Splicing factor 3B subunit 4

Gene

sap-49

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-CEL-72163. mRNA Splicing - Major Pathway.
R-CEL-72165. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor 3B subunit 4
Alternative name(s):
Spliceosome-associated protein 49
Gene namesi
Name:sap-49
ORF Names:C08B11.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiC08B11.5; CE36374; WBGene00004723; sap-49.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Splicing factor 3B subunit 4PRO_0000082013Add
BLAST

Proteomic databases

EPDiQ09442.
PaxDbiQ09442.

PTM databases

iPTMnetiQ09442.

Expressioni

Gene expression databases

ExpressionAtlasiQ09442. baseline.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
sorb-1G5EC324EBI-2316106,EBI-6534380
sorb-1O6248212EBI-2316106,EBI-325337
src-1G5EE563EBI-2316106,EBI-6538807

Protein-protein interaction databases

BioGridi606934. 1 interaction.
IntActiQ09442. 16 interactions.
STRINGi6239.C08B11.5.1.

Structurei

3D structure databases

ProteinModelPortaliQ09442.
SMRiQ09442. Positions 5-206.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 9179RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini100 – 17980RRM 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi200 – 387188Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the SF3B4 family.Curated
Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0131. Eukaryota.
ENOG410XPT4. LUCA.
GeneTreeiENSGT00840000129930.
InParanoidiQ09442.
KOiK12831.
OMAiRMLAKQA.
OrthoDBiEOG77Q4XP.
PhylomeDBiQ09442.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09442-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAGPIVERN QDATIYVGGL DEKVSESILW ELMVQAGPVV SVNMPKDRVT
60 70 80 90 100
ANHQGFGFVE FMGEEDADYA IKILNMIKLY GKPIKVNKAS AHEKNMDVGA
110 120 130 140 150
NIFVGNLDPE VDEKLLYDTF SAFGVILQVP KIMRDVDSGT SKGFAFINFA
160 170 180 190 200
SFEASDTALE AMNGQFLCNR AITVSYAFKR DSKGERHGTA AERMLAAQNP
210 220 230 240 250
LFPKDRPHQV FSDVPLGVPA NTPLAMPGVH AAIAAHATGR PGYQPPPLMG
260 270 280 290 300
MAQSGYQGQY PPVPPPPPSV TPMPPPMPPT PGMTPRPPPP PSSGMWPPPP
310 320 330 340 350
PPPPGRTPGP PGMPGMPPPP PPSRFGPPGM GGMPPPPPPG MRYPGGMPPP
360 370 380
PPPRYPSAGP GMYPPPPPSR PPAPPSGHGM IPPPPPPS
Length:388
Mass (Da):41,008
Last modified:November 23, 2004 - v2
Checksum:i0D6B0CF04CB24C3D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46676 Genomic DNA. Translation: CAB60993.2.
PIRiT19069.
RefSeqiNP_001021932.1. NM_001026761.2.
UniGeneiCel.30808.

Genome annotation databases

EnsemblMetazoaiC08B11.5; C08B11.5; WBGene00004723.
GeneIDi4363023.
KEGGicel:CELE_C08B11.5.
UCSCiC08B11.5.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46676 Genomic DNA. Translation: CAB60993.2.
PIRiT19069.
RefSeqiNP_001021932.1. NM_001026761.2.
UniGeneiCel.30808.

3D structure databases

ProteinModelPortaliQ09442.
SMRiQ09442. Positions 5-206.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi606934. 1 interaction.
IntActiQ09442. 16 interactions.
STRINGi6239.C08B11.5.1.

PTM databases

iPTMnetiQ09442.

Proteomic databases

EPDiQ09442.
PaxDbiQ09442.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC08B11.5; C08B11.5; WBGene00004723.
GeneIDi4363023.
KEGGicel:CELE_C08B11.5.
UCSCiC08B11.5.1. c. elegans.

Organism-specific databases

CTDi4363023.
WormBaseiC08B11.5; CE36374; WBGene00004723; sap-49.

Phylogenomic databases

eggNOGiKOG0131. Eukaryota.
ENOG410XPT4. LUCA.
GeneTreeiENSGT00840000129930.
InParanoidiQ09442.
KOiK12831.
OMAiRMLAKQA.
OrthoDBiEOG77Q4XP.
PhylomeDBiQ09442.

Enzyme and pathway databases

ReactomeiR-CEL-72163. mRNA Splicing - Major Pathway.
R-CEL-72165. mRNA Splicing - Minor Pathway.

Miscellaneous databases

NextBioi883379.
PROiQ09442.

Gene expression databases

ExpressionAtlasiQ09442. baseline.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiSF3B4_CAEEL
AccessioniPrimary (citable) accession number: Q09442
Secondary accession number(s): Q8T8N6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 23, 2004
Last modified: May 11, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.