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Reviewed, UniProtKB/Swiss-Prot Q09429 (ABCC8_RAT)

Last modified January 19, 2010. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    ATP-binding cassette transporter sub-family C member 8
Alternative name(s):
    Sulfonylurea receptor 1
Gene names
Name: Abcc8
Synonyms: Sur, Sur1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length1582 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Putative subunit of the beta-cell ATP-sensitive potassium channel (KATP). Regulator of ATP-sensitive K+ channels and insulin release.

Subunit structure

Associates with KCNJ11 By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. [View classification]

Contains 2 ABC transmembrane type-1 domains.

Contains 2 ABC transporter domains.

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Ontologies

Keywords
   Biological processTransport
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainRepeat
Transmembrane
   LigandATP-binding
Nucleotide-binding
   Molecular functionReceptor
   PTMGlycoprotein
Phosphoprotein
Gene Ontology (GO)
   Biological processnegative regulation of insulin secretion

Inferred from direct assay. Source: RGD

positive regulation of potassium ion transport

Inferred from direct assay. Source: RGD

potassium ion transport

Inferred from electronic annotation. Source: InterPro

response to drug

Inferred from expression pattern. Source: RGD

response to insulin stimulus

Inferred from expression pattern. Source: RGD

response to lipopolysaccharide

Inferred from expression pattern. Source: RGD

response to pH

Inferred from direct assay. Source: RGD

response to zinc ion

Inferred from direct assay. Source: RGD

transmembrane transport

Inferred from electronic annotation. Source: InterPro

   Cellular componentATP-sensitive potassium channel complex

Inferred from mutant phenotype. Source: RGD

sarcolemma

Inferred from direct assay. Source: RGD

synaptic vesicle membrane

Inferred from mutant phenotype. Source: RGD

   Molecular functionATP binding

Inferred from mutant phenotype. Source: RGD

ATPase activity, coupled to transmembrane movement of substances

Inferred from electronic annotation. Source: InterPro

potassium channel activity

Inferred from mutant phenotype. Source: RGD

sulfonylurea receptor activity

Traceable author statement. Source: RGD

syntaxin binding

Inferred from physical interaction. Source: RGD

Complete GO annotation...

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Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.
Isoform A (identifier: Q09429-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B (identifier: Q09429-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1252-1289: Missing.
Isoform C (identifier: Q09429-3)

The sequence of this isoform differs from the canonical sequence as follows:
     741-741: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 15821581ATP-binding cassette transporter sub-family C member 8
PRO_0000093401

Regions

Topological domain2 – 3433Extracellular By similarity
Transmembrane35 – 55211 By similarity
Topological domain56 – 7520Cytoplasmic By similarity
Transmembrane76 – 96212 By similarity
Topological domain97 – 1015Extracellular By similarity
Transmembrane102 – 122213 By similarity
Topological domain123 – 13412Cytoplasmic By similarity
Transmembrane135 – 154204 By similarity
Topological domain155 – 16814Extracellular By similarity
Transmembrane169 – 194265 By similarity
Topological domain195 – 311117Cytoplasmic By similarity
Transmembrane312 – 331206 By similarity
Topological domain332 – 35524Extracellular By similarity
Transmembrane356 – 376217 By similarity
Topological domain377 – 43458Cytoplasmic By similarity
Transmembrane435 – 455218 By similarity
Topological domain456 – 4583Extracellular By similarity
Transmembrane459 – 479219 By similarity
Topological domain480 – 54162Cytoplasmic By similarity
Transmembrane542 – 5622110 By similarity
Topological domain563 – 58422Extracellular By similarity
Transmembrane585 – 6052111 By similarity
Topological domain606 – 1005400Cytoplasmic By similarity
Transmembrane1006 – 10262112 By similarity
Topological domain1027 – 107347Extracellular By similarity
Transmembrane1074 – 10942113 By similarity
Topological domain1095 – 113844Cytoplasmic By similarity
Transmembrane1139 – 11592114 By similarity
Topological domain11601Extracellular By similarity
Transmembrane1161 – 11812115 By similarity
Topological domain1182 – 125271Cytoplasmic By similarity
Transmembrane1253 – 12732116 By similarity
Topological domain1274 – 12774Extracellular By similarity
Transmembrane1278 – 12982117 By similarity
Topological domain1299 – 1582284Cytoplasmic By similarity
Domain299 – 602304ABC transmembrane type-1 1
Domain679 – 930252ABC transporter 1
Domain1013 – 1307295ABC transmembrane type-1 2
Domain1345 – 1579235ABC transporter 2
Nucleotide binding713 – 7208ATP 1 Potential
Nucleotide binding1379 – 13868ATP 2 Potential

Amino acid modifications

Glycosylation101N-linked (GlcNAc...) Ref.5
Glycosylation10501N-linked (GlcNAc...) By similarity

Natural variations

Alternative sequence7411Missing in isoform C.
VSP_000056
Alternative sequence1252 – 128938Missing in isoform B.
VSP_000057

Experimental info

Sequence conflict4871S → T in AAA99237. Ref.1
Sequence conflict6991I → T in BAB19011. Ref.4
Sequence conflict836 – 8372QR → PG in AAA99237. Ref.1
Sequence conflict13141R → G in AAA99237. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 7C57A1CFC86B9214

FASTA1,582177,185
        10         20         30         40         50         60 
MPLAFCGTEN HSAAYRVDQG VLNNGCFVDA LNVVPHVFLL FITFPILFIG WGSQSSKVHI 

        70         80         90        100        110        120 
HHSTWLHFPG HNLRWILTFI LLFVLVCEIA EGILSDGVTE SRHLHLYMPA GMAFMAAITS 

       130        140        150        160        170        180 
VVYYHNIETS NFPKLLIALL IYWTLAFITK TIKFVKFYDH AIGFSQLRFC LTGLLVILYG 

       190        200        210        220        230        240 
MLLLVEVNVI RVRRYVFFKT PREVKPPEDL QDLGVRFLQP FVNLLSKGTY WWMNAFIKTA 

       250        260        270        280        290        300 
HKKPIDLRAI GKLPIAMRAL TNYQRLCLAF DAQARKDTQS QQGARAIWRA LCHAFGRRLV 

       310        320        330        340        350        360 
LSSTFRILAD LLGFAGPLCI FGIVDHLGKE NHVFQPKTQF LGVYFVSSQE FLGNAYVLAV 

       370        380        390        400        410        420 
LLFLALLLQR TFLQASYYVA IETGINLRGA IQTKIYNKIM HLSTSNLSMG EMTAGQICNL 

       430        440        450        460        470        480 
VAIDTNQLMW FFFLCPNLWA MPVQIIVGVI LLYYILGVSA LIGAAVIILL APVQYFVATK 

       490        500        510        520        530        540 
LSQAQRSTLE YSNERLKQTN EMLRGIKLLK LYAWENIFCS RVEKTRRKEM TSLRAFAVYT 

       550        560        570        580        590        600 
SISIFMNTAI PIAAVLITFV GHVSFFKESD FSPSVAFASL SLFHILVTPL FLLSSVVRST 

       610        620        630        640        650        660 
VKALVSVQKL SEFLSSAEIR EEQCAPREPA PQGQAGKYQA VPLKVVNRKR PAREEVRDLL 

       670        680        690        700        710        720 
GPLQRLTPST DGDADNFCVQ IIGGFFTWTP DGIPTLSNIT IRIPRGQLTM IVGQVGCGKS 

       730        740        750        760        770        780 
SLLLATLGEM QKVSGAVFWN SSLPDSEGED PSNPERETAA DSDARSRGPV AYASQKPWLL 

       790        800        810        820        830        840 
NATVEENITF ESPFNKQRYK MVIEACSLQP DIDILPHGDQ TQIGERGINL SGGQRQRISV 

       850        860        870        880        890        900 
ARALYQHTNV VFLDDPFSAL DVHLSDHLMQ AGILELLRDD KRTVVLVTHK LQYLPHADWI 

       910        920        930        940        950        960 
IAMKDGTIQR EGTLKDFQRS ECQLFEHWKT LMNRQDQELE KETVMERKAP EPSQGLPRAM 

       970        980        990       1000       1010       1020 
SSRDGLLLDE DEEEEEAAES EEDDNLSSVL HQRAKIPWRA CTKYLSSAGI LLLSLLVFSQ 

      1030       1040       1050       1060       1070       1080 
LLKHMVLVAI DYWLAKWTDS ALVLSPAARN CSLSQECALD QSVYAMVFTV LCSLGIALCL 

      1090       1100       1110       1120       1130       1140 
VTSVTVEWTG LKVAKRLHRS LLNRIILAPM RFFETTPLGS ILNRFSSDCN TIDQHIPSTL 

      1150       1160       1170       1180       1190       1200 
ECLSRSTLLC VSALAVISYV TPVFLVALLP LAVVCYFIQK YFRVASRDLQ QLDDTTQLPL 

      1210       1220       1230       1240       1250       1260 
LSHFAETVEG LTTIRAFRYE ARFQQKLLEY TDSNNIASLF LTAANRWLEV RMEYIGACVV 

      1270       1280       1290       1300       1310       1320 
LIAAATSISN SLHRELSAGL VGLGLTYALM VSNYLNWMVR NLADMEIQLG AVKRIHTLLK 

      1330       1340       1350       1360       1370       1380 
TEAESYEGLL APSLIPKNWP DQGKIQIQNL SVRYDSSLKP VLKHVNALIS PGQKIGICGR 

      1390       1400       1410       1420       1430       1440 
TGSGKSSFSL AFFRMVDMFE GRIIIDGIDI AKLPLHTLRS RLSIILQDPV LFSGTIRFNL 

      1450       1460       1470       1480       1490       1500 
DPEKKCSDST LWEALEIAQL KLVVKALPGG LDAIITEGGE NFSQGQRQLF CLARAFVRKT 

      1510       1520       1530       1540       1550       1560 
SIFIMDEATA SIDMATENIL QKVVMTAFAD RTVVTIAHRV HTILSADLVM VLKRGAILEF 

      1570       1580 
DKPEKLLSQK DSVFASFVRA DK

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Isoform B.

Checksum: 338163E4F264FCFE
Show »

FASTA1,544173,324
Isoform C.

Checksum: 722EC6057F4333BC
Show »

FASTA1,581177,097

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References

[1]"Cloning of the beta cell high-affinity sulfonylurea receptor: a regulator of insulin secretion."
Aguilar-Bryan L., Nichols C.G., Wechsler S.W., Clement J.P. IV, Boyd A.E. III, Gonzalez G., Herrera-Sosa H., Nguy K., Bryan J., Nelson D.A.
Science 268:423-426(1995) [PubMed: 7716547] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C).
Tissue: Pancreatic islet.
[2]Faure C., Partiseti M., Gouhier C., Graham D.
Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
Tissue: Pancreatic islet.
[3]"Identification of two forms of sulfonylurea receptors (SUR 1A and SUR 1B) by means of polymerase chain reaction, subcloning, DNA sequencing and tissue distribution."
Blache P., Peyrollier K., Gros L., Bataille D.
Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
Tissue: Pancreatic islet.
[4]Cao K., Wang R.
Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
Tissue: Vascular smooth muscle.
[5]"The high-affinity sulfonylurea receptor: distribution, glycosylation, purification, and immunoprecipitation of two forms from endocrine and neuroendocrine cell lines."
Nelson D.A., Bryan J., Wechsler S., Clement J.P. IV, Aguilar-Bryan L.
Biochemistry 35:14793-14799(1996) [PubMed: 8942641] [Abstract]
Cited for: GLYCOSYLATION AT ASN-10.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L40624 mRNA. Translation: AAA99237.1.
X97279 mRNA. Translation: CAA65934.1.
AF039595 mRNA. Translation: AAB96684.1.
AB052294 mRNA. Translation: BAB19011.1.
IPIIPI00204427.
IPI00476240.
IPI00851131.
RefSeqNP_037171.2.
UniGeneRn.11187

3D structure databases

SMRQ09429. Positions 285-921, 1078-1579.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ09429.

Genome annotation databases

EnsemblENSRNOT00000028696; ENSRNOP00000028696; ENSRNOG00000021130; Rattus norvegicus. [Genome view]
GeneID25559.
KEGGrno:25559.

Organism-specific databases

CTD25559.
RGD3786. Abcc8.

Phylogenomic databases

eggNOGmaNOG08054.
HOVERGENQ09429.
PhylomeDBQ09429.

Gene expression databases

ArrayExpressQ09429.
GenevestigatorQ09429.
GermOnlineENSRNOG00000021130. Rattus norvegicus.

Family and domain databases

InterProIPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR017940. ABC_transporter_type1.
IPR001140. ABC_transptr_TM_dom.
IPR011527. ABC_transptrTM_dom_typ1.
IPR003593. ATPase_AAA+_core.
IPR000388. Sulphorea_rcpt_N.
IPR000844. Surea_rcpt_1_N.
[Graphical view]
PfamPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
PRINTSPR01093. SULFNYLUR1.
PR01092. SULFNYLUREAR.
SMARTSM00382. AAA. 2 hits.
[Graphical view]
PROSITEPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio607139.

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Entry information

Entry nameABCC8_RAT
AccessionPrimary (citable) accession number: Q09429
Secondary accession number(s): O54989, P70532, Q9EQT0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: January 19, 2010
This is version 86 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents