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Q09426

- CGT_RAT

UniProt

Q09426 - CGT_RAT

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Protein

2-hydroxyacylsphingosine 1-beta-galactosyltransferase

Gene
Ugt8, Cgt, Ugt4
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system.

Catalytic activityi

UDP-alpha-D-galactose + 2-(2-hydroxyacyl)sphingosine = UDP + 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine.

Pathwayi

GO - Molecular functioni

  1. 2-hydroxyacylsphingosine 1-beta-galactosyltransferase activity Source: UniProtKB-EC
  2. UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity Source: RGD

GO - Biological processi

  1. axon cargo transport Source: Ensembl
  2. cytoskeleton organization Source: Ensembl
  3. galactosylceramide biosynthetic process Source: UniProtKB-UniPathway
  4. glycosphingolipid biosynthetic process Source: RGD
  5. myelination Source: RGD
  6. neuron projection morphogenesis Source: Ensembl
  7. paranodal junction assembly Source: Ensembl
  8. protein localization to paranode region of axon Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00787.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
2-hydroxyacylsphingosine 1-beta-galactosyltransferase (EC:2.4.1.45)
Alternative name(s):
Ceramide UDP-galactosyltransferase
Cerebroside synthase
UDP-galactose-ceramide galactosyltransferase
Gene namesi
Name:Ugt8
Synonyms:Cgt, Ugt4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 2

Organism-specific databases

RGDi3938. Ugt8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei472 – 49221Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed predictionAdd
BLAST
Chaini21 – 5415212-hydroxyacylsphingosine 1-beta-galactosyltransferasePRO_0000036066Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi78 – 781N-linked (GlcNAc...) Reviewed prediction1 Publication
Glycosylationi333 – 3331N-linked (GlcNAc...) Reviewed prediction1 Publication
Glycosylationi442 – 4421N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ09426.
PRIDEiQ09426.

Expressioni

Tissue specificityi

Brain, restricted to the oligodendrocyte-containing cell layers of cerebrum and cerebellum.

Gene expression databases

GenevestigatoriQ09426.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012676.

Structurei

3D structure databases

ProteinModelPortaliQ09426.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1819.
GeneTreeiENSGT00560000076760.
HOGENOMiHOG000220831.
HOVERGENiHBG098341.
InParanoidiQ09426.
KOiK04628.
OMAiSNHYSLQ.
OrthoDBiEOG7GBFWS.
PhylomeDBiQ09426.
TreeFamiTF315472.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q09426-1 [UniParc]FASTAAdd to Basket

« Hide

MKSYTPYFML LWSAVGIARA AKIIIVPPIM FESHLYIFKT LASALHERGH    50
HTVFLLSEGR DIDPSNHYSL QRYPGIFNST TSDAFLQSKM RNIFSGRLTA 100
VELVDILDHY TKNCDMMVGN QALIQGLKKE KFDLLLVDPN DMCGFVIAHL 150
LGVKYAVFST GLWYPAEVGA PAPLAYVPEF NSLLTDRMNF LERMKNTGVY 200
LISRMGVSFL VLPKYERIMQ KYNLLPAKSM YDLVHGSSLW MLCTDVALEF 250
PRPTLPNVVY VGGILTKPAS PLPEDLQRWV DGAQEHGFVL VSFGAGVKYL 300
SEDIANKLAG ALGRLPQKVI WRFSGTKPKN LGNNTKLIEW LPQNDLLGHS 350
NIRAFLSHGG LNSIFETMYH GVPVVGIPLF GDHYDTMTRV QAKGMGILLE 400
WNTVTEGELY DALVKVINNP SYRQRAQKLS EIHKDQPGHP VNRTTYWIDY 450
ILRHDGAHHL RSAVHQISFC QYFLLDIAFV LLLGAVALYF IVSYVTKFIY 500
RKVKSLCSRS THSTVNGHYQ NGILNGRYKG NGHIKHEKKV K 541
Length:541
Mass (Da):61,126
Last modified:November 1, 1995 - v1
Checksum:i260D7603170151BB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L21698 mRNA. Translation: AAA16108.1.
U07683 mRNA. Translation: AAA50212.1.
PIRiA48801.
RefSeqiNP_062149.1. NM_019276.3.
UniGeneiRn.9744.

Genome annotation databases

EnsembliENSRNOT00000012676; ENSRNOP00000012676; ENSRNOG00000009345.
GeneIDi50555.
KEGGirno:50555.
UCSCiRGD:3938. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L21698 mRNA. Translation: AAA16108.1 .
U07683 mRNA. Translation: AAA50212.1 .
PIRi A48801.
RefSeqi NP_062149.1. NM_019276.3.
UniGenei Rn.9744.

3D structure databases

ProteinModelPortali Q09426.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000012676.

Protein family/group databases

CAZyi GT1. Glycosyltransferase Family 1.

Proteomic databases

PaxDbi Q09426.
PRIDEi Q09426.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000012676 ; ENSRNOP00000012676 ; ENSRNOG00000009345 .
GeneIDi 50555.
KEGGi rno:50555.
UCSCi RGD:3938. rat.

Organism-specific databases

CTDi 7368.
RGDi 3938. Ugt8.

Phylogenomic databases

eggNOGi COG1819.
GeneTreei ENSGT00560000076760.
HOGENOMi HOG000220831.
HOVERGENi HBG098341.
InParanoidi Q09426.
KOi K04628.
OMAi SNHYSLQ.
OrthoDBi EOG7GBFWS.
PhylomeDBi Q09426.
TreeFami TF315472.

Enzyme and pathway databases

UniPathwayi UPA00787 .

Miscellaneous databases

NextBioi 610356.
PROi Q09426.

Gene expression databases

Genevestigatori Q09426.

Family and domain databases

InterProi IPR002213. UDP_glucos_trans.
[Graphical view ]
PANTHERi PTHR11926. PTHR11926. 1 hit.
Pfami PF00201. UDPGT. 1 hit.
[Graphical view ]
PROSITEi PS00375. UDPGT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Ceramide UDPgalactosyltransferase from myelinating rat brain: purification, cloning, and expression."
    Schulte S., Stoffel W.
    Proc. Natl. Acad. Sci. U.S.A. 90:10265-10269(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.
  2. "Isolation, characterization, and expression of cDNA clones that encode rat UDP-galactose: ceramide galactosyltransferase."
    Stahl N., Jurevics H., Morell P., Suzuki K., Popko B.
    J. Neurosci. Res. 38:234-242(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiCGT_RAT
AccessioniPrimary (citable) accession number: Q09426
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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