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Protein

2-hydroxyacylsphingosine 1-beta-galactosyltransferase

Gene

Ugt8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system.

Catalytic activityi

UDP-alpha-D-galactose + 2-(2-hydroxyacyl)sphingosine = UDP + 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine.

Pathwayi: galactosylceramide biosynthesis

This protein is involved in the pathway galactosylceramide biosynthesis, which is part of Sphingolipid metabolism.
View all proteins of this organism that are known to be involved in the pathway galactosylceramide biosynthesis and in Sphingolipid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

ReactomeiR-RNO-1660662. Glycosphingolipid metabolism.
UniPathwayiUPA00787.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Chemistry databases

SwissLipidsiSLP:000000910.

Names & Taxonomyi

Protein namesi
Recommended name:
2-hydroxyacylsphingosine 1-beta-galactosyltransferase (EC:2.4.1.45)
Alternative name(s):
Ceramide UDP-galactosyltransferase
Cerebroside synthase
UDP-galactose-ceramide galactosyltransferase
Gene namesi
Name:Ugt8
Synonyms:Cgt, Ugt4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi3938. Ugt8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei472 – 492HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000003606621 – 5412-hydroxyacylsphingosine 1-beta-galactosyltransferaseAdd BLAST521

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi78N-linked (GlcNAc...)1 PublicationSequence analysis1
Glycosylationi333N-linked (GlcNAc...)1 PublicationSequence analysis1
Glycosylationi442N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ09426.
PRIDEiQ09426.

Expressioni

Tissue specificityi

Brain, restricted to the oligodendrocyte-containing cell layers of cerebrum and cerebellum.

Gene expression databases

BgeeiENSRNOG00000009345.
GenevisibleiQ09426. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012676.

Structurei

3D structure databases

ProteinModelPortaliQ09426.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1192. Eukaryota.
COG1819. LUCA.
GeneTreeiENSGT00760000118949.
HOGENOMiHOG000220831.
HOVERGENiHBG098341.
InParanoidiQ09426.
KOiK04628.
OMAiIAPSNHY.
OrthoDBiEOG091G06JC.
PhylomeDBiQ09426.
TreeFamiTF315472.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q09426-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSYTPYFML LWSAVGIARA AKIIIVPPIM FESHLYIFKT LASALHERGH
60 70 80 90 100
HTVFLLSEGR DIDPSNHYSL QRYPGIFNST TSDAFLQSKM RNIFSGRLTA
110 120 130 140 150
VELVDILDHY TKNCDMMVGN QALIQGLKKE KFDLLLVDPN DMCGFVIAHL
160 170 180 190 200
LGVKYAVFST GLWYPAEVGA PAPLAYVPEF NSLLTDRMNF LERMKNTGVY
210 220 230 240 250
LISRMGVSFL VLPKYERIMQ KYNLLPAKSM YDLVHGSSLW MLCTDVALEF
260 270 280 290 300
PRPTLPNVVY VGGILTKPAS PLPEDLQRWV DGAQEHGFVL VSFGAGVKYL
310 320 330 340 350
SEDIANKLAG ALGRLPQKVI WRFSGTKPKN LGNNTKLIEW LPQNDLLGHS
360 370 380 390 400
NIRAFLSHGG LNSIFETMYH GVPVVGIPLF GDHYDTMTRV QAKGMGILLE
410 420 430 440 450
WNTVTEGELY DALVKVINNP SYRQRAQKLS EIHKDQPGHP VNRTTYWIDY
460 470 480 490 500
ILRHDGAHHL RSAVHQISFC QYFLLDIAFV LLLGAVALYF IVSYVTKFIY
510 520 530 540
RKVKSLCSRS THSTVNGHYQ NGILNGRYKG NGHIKHEKKV K
Length:541
Mass (Da):61,126
Last modified:November 1, 1995 - v1
Checksum:i260D7603170151BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L21698 mRNA. Translation: AAA16108.1.
U07683 mRNA. Translation: AAA50212.1.
PIRiA48801.
RefSeqiNP_062149.1. NM_019276.3.
XP_017446541.1. XM_017591052.1.
UniGeneiRn.9744.

Genome annotation databases

EnsembliENSRNOT00000012676; ENSRNOP00000012676; ENSRNOG00000009345.
GeneIDi50555.
KEGGirno:50555.
UCSCiRGD:3938. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L21698 mRNA. Translation: AAA16108.1.
U07683 mRNA. Translation: AAA50212.1.
PIRiA48801.
RefSeqiNP_062149.1. NM_019276.3.
XP_017446541.1. XM_017591052.1.
UniGeneiRn.9744.

3D structure databases

ProteinModelPortaliQ09426.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012676.

Chemistry databases

SwissLipidsiSLP:000000910.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Proteomic databases

PaxDbiQ09426.
PRIDEiQ09426.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012676; ENSRNOP00000012676; ENSRNOG00000009345.
GeneIDi50555.
KEGGirno:50555.
UCSCiRGD:3938. rat.

Organism-specific databases

CTDi7368.
RGDi3938. Ugt8.

Phylogenomic databases

eggNOGiKOG1192. Eukaryota.
COG1819. LUCA.
GeneTreeiENSGT00760000118949.
HOGENOMiHOG000220831.
HOVERGENiHBG098341.
InParanoidiQ09426.
KOiK04628.
OMAiIAPSNHY.
OrthoDBiEOG091G06JC.
PhylomeDBiQ09426.
TreeFamiTF315472.

Enzyme and pathway databases

UniPathwayiUPA00787.
ReactomeiR-RNO-1660662. Glycosphingolipid metabolism.

Miscellaneous databases

PROiQ09426.

Gene expression databases

BgeeiENSRNOG00000009345.
GenevisibleiQ09426. RN.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCGT_RAT
AccessioniPrimary (citable) accession number: Q09426
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.