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Q09426

- CGT_RAT

UniProt

Q09426 - CGT_RAT

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Protein

2-hydroxyacylsphingosine 1-beta-galactosyltransferase

Gene

Ugt8

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system.

Catalytic activityi

UDP-alpha-D-galactose + 2-(2-hydroxyacyl)sphingosine = UDP + 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine.

Pathwayi

GO - Molecular functioni

  1. 2-hydroxyacylsphingosine 1-beta-galactosyltransferase activity Source: UniProtKB-EC
  2. UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity Source: RGD

GO - Biological processi

  1. axon cargo transport Source: Ensembl
  2. cytoskeleton organization Source: Ensembl
  3. galactosylceramide biosynthetic process Source: UniProtKB-UniPathway
  4. glycosphingolipid biosynthetic process Source: RGD
  5. myelination Source: RGD
  6. neuron projection morphogenesis Source: Ensembl
  7. paranodal junction assembly Source: Ensembl
  8. protein localization to paranode region of axon Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00787.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
2-hydroxyacylsphingosine 1-beta-galactosyltransferase (EC:2.4.1.45)
Alternative name(s):
Ceramide UDP-galactosyltransferase
Cerebroside synthase
UDP-galactose-ceramide galactosyltransferase
Gene namesi
Name:Ugt8
Synonyms:Cgt, Ugt4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 2

Organism-specific databases

RGDi3938. Ugt8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei472 – 49221HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 5415212-hydroxyacylsphingosine 1-beta-galactosyltransferasePRO_0000036066Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi78 – 781N-linked (GlcNAc...)1 PublicationSequence Analysis
Glycosylationi333 – 3331N-linked (GlcNAc...)1 PublicationSequence Analysis
Glycosylationi442 – 4421N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ09426.
PRIDEiQ09426.

Expressioni

Tissue specificityi

Brain, restricted to the oligodendrocyte-containing cell layers of cerebrum and cerebellum.

Gene expression databases

GenevestigatoriQ09426.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012676.

Structurei

3D structure databases

ProteinModelPortaliQ09426.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the UDP-glycosyltransferase family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1819.
GeneTreeiENSGT00760000118949.
HOGENOMiHOG000220831.
HOVERGENiHBG098341.
InParanoidiQ09426.
KOiK04628.
OMAiSNHYSLQ.
OrthoDBiEOG7GBFWS.
PhylomeDBiQ09426.
TreeFamiTF315472.

Family and domain databases

InterProiIPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF00201. UDPGT. 1 hit.
[Graphical view]
PROSITEiPS00375. UDPGT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q09426-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSYTPYFML LWSAVGIARA AKIIIVPPIM FESHLYIFKT LASALHERGH
60 70 80 90 100
HTVFLLSEGR DIDPSNHYSL QRYPGIFNST TSDAFLQSKM RNIFSGRLTA
110 120 130 140 150
VELVDILDHY TKNCDMMVGN QALIQGLKKE KFDLLLVDPN DMCGFVIAHL
160 170 180 190 200
LGVKYAVFST GLWYPAEVGA PAPLAYVPEF NSLLTDRMNF LERMKNTGVY
210 220 230 240 250
LISRMGVSFL VLPKYERIMQ KYNLLPAKSM YDLVHGSSLW MLCTDVALEF
260 270 280 290 300
PRPTLPNVVY VGGILTKPAS PLPEDLQRWV DGAQEHGFVL VSFGAGVKYL
310 320 330 340 350
SEDIANKLAG ALGRLPQKVI WRFSGTKPKN LGNNTKLIEW LPQNDLLGHS
360 370 380 390 400
NIRAFLSHGG LNSIFETMYH GVPVVGIPLF GDHYDTMTRV QAKGMGILLE
410 420 430 440 450
WNTVTEGELY DALVKVINNP SYRQRAQKLS EIHKDQPGHP VNRTTYWIDY
460 470 480 490 500
ILRHDGAHHL RSAVHQISFC QYFLLDIAFV LLLGAVALYF IVSYVTKFIY
510 520 530 540
RKVKSLCSRS THSTVNGHYQ NGILNGRYKG NGHIKHEKKV K
Length:541
Mass (Da):61,126
Last modified:November 1, 1995 - v1
Checksum:i260D7603170151BB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L21698 mRNA. Translation: AAA16108.1.
U07683 mRNA. Translation: AAA50212.1.
PIRiA48801.
RefSeqiNP_062149.1. NM_019276.3.
UniGeneiRn.9744.

Genome annotation databases

EnsembliENSRNOT00000012676; ENSRNOP00000012676; ENSRNOG00000009345.
GeneIDi50555.
KEGGirno:50555.
UCSCiRGD:3938. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L21698 mRNA. Translation: AAA16108.1 .
U07683 mRNA. Translation: AAA50212.1 .
PIRi A48801.
RefSeqi NP_062149.1. NM_019276.3.
UniGenei Rn.9744.

3D structure databases

ProteinModelPortali Q09426.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000012676.

Protein family/group databases

CAZyi GT1. Glycosyltransferase Family 1.

Proteomic databases

PaxDbi Q09426.
PRIDEi Q09426.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000012676 ; ENSRNOP00000012676 ; ENSRNOG00000009345 .
GeneIDi 50555.
KEGGi rno:50555.
UCSCi RGD:3938. rat.

Organism-specific databases

CTDi 7368.
RGDi 3938. Ugt8.

Phylogenomic databases

eggNOGi COG1819.
GeneTreei ENSGT00760000118949.
HOGENOMi HOG000220831.
HOVERGENi HBG098341.
InParanoidi Q09426.
KOi K04628.
OMAi SNHYSLQ.
OrthoDBi EOG7GBFWS.
PhylomeDBi Q09426.
TreeFami TF315472.

Enzyme and pathway databases

UniPathwayi UPA00787 .

Miscellaneous databases

NextBioi 610356.
PROi Q09426.

Gene expression databases

Genevestigatori Q09426.

Family and domain databases

InterProi IPR002213. UDP_glucos_trans.
[Graphical view ]
PANTHERi PTHR11926. PTHR11926. 1 hit.
Pfami PF00201. UDPGT. 1 hit.
[Graphical view ]
PROSITEi PS00375. UDPGT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Ceramide UDPgalactosyltransferase from myelinating rat brain: purification, cloning, and expression."
    Schulte S., Stoffel W.
    Proc. Natl. Acad. Sci. U.S.A. 90:10265-10269(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Brain.
  2. "Isolation, characterization, and expression of cDNA clones that encode rat UDP-galactose: ceramide galactosyltransferase."
    Stahl N., Jurevics H., Morell P., Suzuki K., Popko B.
    J. Neurosci. Res. 38:234-242(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiCGT_RAT
AccessioniPrimary (citable) accession number: Q09426
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3