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Q09426

- CGT_RAT

UniProt

Q09426 - CGT_RAT

Protein

2-hydroxyacylsphingosine 1-beta-galactosyltransferase

Gene

Ugt8

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system.

    Catalytic activityi

    UDP-alpha-D-galactose + 2-(2-hydroxyacyl)sphingosine = UDP + 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine.

    Pathwayi

    GO - Molecular functioni

    1. 2-hydroxyacylsphingosine 1-beta-galactosyltransferase activity Source: UniProtKB-EC
    2. UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity Source: RGD

    GO - Biological processi

    1. axon cargo transport Source: Ensembl
    2. cytoskeleton organization Source: Ensembl
    3. galactosylceramide biosynthetic process Source: UniProtKB-UniPathway
    4. glycosphingolipid biosynthetic process Source: RGD
    5. myelination Source: RGD
    6. neuron projection morphogenesis Source: Ensembl
    7. paranodal junction assembly Source: Ensembl
    8. protein localization to paranode region of axon Source: Ensembl

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Lipid metabolism, Sphingolipid metabolism

    Enzyme and pathway databases

    UniPathwayiUPA00787.

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-hydroxyacylsphingosine 1-beta-galactosyltransferase (EC:2.4.1.45)
    Alternative name(s):
    Ceramide UDP-galactosyltransferase
    Cerebroside synthase
    UDP-galactose-ceramide galactosyltransferase
    Gene namesi
    Name:Ugt8
    Synonyms:Cgt, Ugt4
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi3938. Ugt8.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 5415212-hydroxyacylsphingosine 1-beta-galactosyltransferasePRO_0000036066Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi78 – 781N-linked (GlcNAc...)1 PublicationSequence Analysis
    Glycosylationi333 – 3331N-linked (GlcNAc...)1 PublicationSequence Analysis
    Glycosylationi442 – 4421N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ09426.
    PRIDEiQ09426.

    Expressioni

    Tissue specificityi

    Brain, restricted to the oligodendrocyte-containing cell layers of cerebrum and cerebellum.

    Gene expression databases

    GenevestigatoriQ09426.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000012676.

    Structurei

    3D structure databases

    ProteinModelPortaliQ09426.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei472 – 49221HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the UDP-glycosyltransferase family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1819.
    GeneTreeiENSGT00560000076760.
    HOGENOMiHOG000220831.
    HOVERGENiHBG098341.
    InParanoidiQ09426.
    KOiK04628.
    OMAiSNHYSLQ.
    OrthoDBiEOG7GBFWS.
    PhylomeDBiQ09426.
    TreeFamiTF315472.

    Family and domain databases

    InterProiIPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 1 hit.
    PfamiPF00201. UDPGT. 1 hit.
    [Graphical view]
    PROSITEiPS00375. UDPGT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q09426-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSYTPYFML LWSAVGIARA AKIIIVPPIM FESHLYIFKT LASALHERGH    50
    HTVFLLSEGR DIDPSNHYSL QRYPGIFNST TSDAFLQSKM RNIFSGRLTA 100
    VELVDILDHY TKNCDMMVGN QALIQGLKKE KFDLLLVDPN DMCGFVIAHL 150
    LGVKYAVFST GLWYPAEVGA PAPLAYVPEF NSLLTDRMNF LERMKNTGVY 200
    LISRMGVSFL VLPKYERIMQ KYNLLPAKSM YDLVHGSSLW MLCTDVALEF 250
    PRPTLPNVVY VGGILTKPAS PLPEDLQRWV DGAQEHGFVL VSFGAGVKYL 300
    SEDIANKLAG ALGRLPQKVI WRFSGTKPKN LGNNTKLIEW LPQNDLLGHS 350
    NIRAFLSHGG LNSIFETMYH GVPVVGIPLF GDHYDTMTRV QAKGMGILLE 400
    WNTVTEGELY DALVKVINNP SYRQRAQKLS EIHKDQPGHP VNRTTYWIDY 450
    ILRHDGAHHL RSAVHQISFC QYFLLDIAFV LLLGAVALYF IVSYVTKFIY 500
    RKVKSLCSRS THSTVNGHYQ NGILNGRYKG NGHIKHEKKV K 541
    Length:541
    Mass (Da):61,126
    Last modified:November 1, 1995 - v1
    Checksum:i260D7603170151BB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L21698 mRNA. Translation: AAA16108.1.
    U07683 mRNA. Translation: AAA50212.1.
    PIRiA48801.
    RefSeqiNP_062149.1. NM_019276.3.
    UniGeneiRn.9744.

    Genome annotation databases

    EnsembliENSRNOT00000012676; ENSRNOP00000012676; ENSRNOG00000009345.
    GeneIDi50555.
    KEGGirno:50555.
    UCSCiRGD:3938. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L21698 mRNA. Translation: AAA16108.1 .
    U07683 mRNA. Translation: AAA50212.1 .
    PIRi A48801.
    RefSeqi NP_062149.1. NM_019276.3.
    UniGenei Rn.9744.

    3D structure databases

    ProteinModelPortali Q09426.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000012676.

    Protein family/group databases

    CAZyi GT1. Glycosyltransferase Family 1.

    Proteomic databases

    PaxDbi Q09426.
    PRIDEi Q09426.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000012676 ; ENSRNOP00000012676 ; ENSRNOG00000009345 .
    GeneIDi 50555.
    KEGGi rno:50555.
    UCSCi RGD:3938. rat.

    Organism-specific databases

    CTDi 7368.
    RGDi 3938. Ugt8.

    Phylogenomic databases

    eggNOGi COG1819.
    GeneTreei ENSGT00560000076760.
    HOGENOMi HOG000220831.
    HOVERGENi HBG098341.
    InParanoidi Q09426.
    KOi K04628.
    OMAi SNHYSLQ.
    OrthoDBi EOG7GBFWS.
    PhylomeDBi Q09426.
    TreeFami TF315472.

    Enzyme and pathway databases

    UniPathwayi UPA00787 .

    Miscellaneous databases

    NextBioi 610356.
    PROi Q09426.

    Gene expression databases

    Genevestigatori Q09426.

    Family and domain databases

    InterProi IPR002213. UDP_glucos_trans.
    [Graphical view ]
    PANTHERi PTHR11926. PTHR11926. 1 hit.
    Pfami PF00201. UDPGT. 1 hit.
    [Graphical view ]
    PROSITEi PS00375. UDPGT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Ceramide UDPgalactosyltransferase from myelinating rat brain: purification, cloning, and expression."
      Schulte S., Stoffel W.
      Proc. Natl. Acad. Sci. U.S.A. 90:10265-10269(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Brain.
    2. "Isolation, characterization, and expression of cDNA clones that encode rat UDP-galactose: ceramide galactosyltransferase."
      Stahl N., Jurevics H., Morell P., Suzuki K., Popko B.
      J. Neurosci. Res. 38:234-242(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Brain.

    Entry informationi

    Entry nameiCGT_RAT
    AccessioniPrimary (citable) accession number: Q09426
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3