2-hydroxyacylsphingosine 1-beta-galactosyltransferase precursor

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Q09426 (CGT_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
2-hydroxyacylsphingosine 1-beta-galactosyltransferase
EC=2.4.1.45

Alternative name(s):
Ceramide UDP-galactosyltransferase
Cerebroside synthase
UDP-galactose-ceramide galactosyltransferase

Gene names

Name:	Ugt8
Synonyms:	Cgt, Ugt4

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	541 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central nervous system and peripheral nervous system.
Catalytic activity	UDP-alpha-D-galactose + 2-(2-hydroxyacyl)sphingosine = UDP + 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine.
Pathway	Sphingolipid metabolism; galactosylceramide biosynthesis.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Tissue specificity	Brain, restricted to the oligodendrocyte-containing cell layers of cerebrum and cerebellum.
Sequence similarities	Belongs to the UDP-glycosyltransferase family.

Ontologies

Keywords
Biological process	Lipid metabolism Sphingolipid metabolism
Cellular component	Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axon cargo transport Inferred from electronic annotation. Source: Compara cytoskeleton organization Inferred from electronic annotation. Source: Compara galactosylceramide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glycosphingolipid biosynthetic process Traceable author statement Ref.2. Source: RGD myelination Traceable author statement Ref.2. Source: RGD neuron projection morphogenesis Inferred from electronic annotation. Source: Compara paranodal junction assembly Inferred from electronic annotation. Source: Compara protein localization to paranode region of axon Inferred from electronic annotation. Source: Compara
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	2-hydroxyacylsphingosine 1-beta-galactosyltransferase activity Inferred from electronic annotation. Source: EC UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity Inferred from mutant phenotype Ref.2. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 20

Potential

Chain

21 – 541

521

2-hydroxyacylsphingosine 1-beta-galactosyltransferase

PRO_0000036066

Regions

Transmembrane

472 – 492

Helical; Potential

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential Ref.1

Glycosylation

333

N-linked (GlcNAc...) Potential Ref.1

Glycosylation

442

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q09426 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 260D7603170151BB
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FASTA

541

61,126

        10         20         30         40         50         60 
MKSYTPYFML LWSAVGIARA AKIIIVPPIM FESHLYIFKT LASALHERGH HTVFLLSEGR 

        70         80         90        100        110        120 
DIDPSNHYSL QRYPGIFNST TSDAFLQSKM RNIFSGRLTA VELVDILDHY TKNCDMMVGN 

       130        140        150        160        170        180 
QALIQGLKKE KFDLLLVDPN DMCGFVIAHL LGVKYAVFST GLWYPAEVGA PAPLAYVPEF 

       190        200        210        220        230        240 
NSLLTDRMNF LERMKNTGVY LISRMGVSFL VLPKYERIMQ KYNLLPAKSM YDLVHGSSLW 

       250        260        270        280        290        300 
MLCTDVALEF PRPTLPNVVY VGGILTKPAS PLPEDLQRWV DGAQEHGFVL VSFGAGVKYL 

       310        320        330        340        350        360 
SEDIANKLAG ALGRLPQKVI WRFSGTKPKN LGNNTKLIEW LPQNDLLGHS NIRAFLSHGG 

       370        380        390        400        410        420 
LNSIFETMYH GVPVVGIPLF GDHYDTMTRV QAKGMGILLE WNTVTEGELY DALVKVINNP 

       430        440        450        460        470        480 
SYRQRAQKLS EIHKDQPGHP VNRTTYWIDY ILRHDGAHHL RSAVHQISFC QYFLLDIAFV 

       490        500        510        520        530        540 
LLLGAVALYF IVSYVTKFIY RKVKSLCSRS THSTVNGHYQ NGILNGRYKG NGHIKHEKKV 


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References

[1]	"Ceramide UDPgalactosyltransferase from myelinating rat brain: purification, cloning, and expression." Schulte S., Stoffel W. Proc. Natl. Acad. Sci. U.S.A. 90:10265-10269(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Brain.
[2]	"Isolation, characterization, and expression of cDNA clones that encode rat UDP-galactose: ceramide galactosyltransferase." Stahl N., Jurevics H., Morell P., Suzuki K., Popko B. J. Neurosci. Res. 38:234-242(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L21698 mRNA. Translation: AAA16108.1. U07683 mRNA. Translation: AAA50212.1.
IPI	IPI00204426.
PIR	A48801.
RefSeq	NP_062149.1. NM_019276.3.
UniGene	Rn.9744.
3D structure databases
ProteinModelPortal	Q09426.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000012676.
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Proteomic databases
PaxDb	Q09426.
PRIDE	Q09426.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000012676; ENSRNOP00000012676; ENSRNOG00000009345.
GeneID	50555.
KEGG	rno:50555.
UCSC	RGD:3938. rat.
Organism-specific databases
CTD	7368.
RGD	3938. Ugt8.
Phylogenomic databases
eggNOG	COG1819.
GeneTree	ENSGT00560000076760.
HOGENOM	HOG000220831.
HOVERGEN	HBG098341.
InParanoid	Q09426.
KO	K04628.
OMA	NHYSLQR.
OrthoDB	EOG4KKZ2Q.
Enzyme and pathway databases
UniPathway	UPA00787.
Gene expression databases
Genevestigator	Q09426.
GermOnline	ENSRNOG00000009345. Rattus norvegicus.
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
PANTHER	PTHR11926. PTHR11926. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	610356.

Entry information

Entry name

CGT_RAT

Accession

Primary (citable) accession number: Q09426

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents