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Protein

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase sqv-8

Gene

sqv-8

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glycosyltransferase required for the biosynthesis of the tetrasaccharide (GlcA-Gal-Gal-Xyl-)Ser core linker of heparan sulfate and chondroitin sulfate (PubMed:11005858). May be involved in the biosynthesis of the HNK-1 carbohydrate epitope on glycoproteins (PubMed:11005858). Required for embryonic development (PubMed:9927677). Involved in the elongation of the pharyngeal isthmus during the later stages of embryonic development (PubMed:16828468). Involved in vulval epithelium invagination (PubMed:9927677).3 Publications

Catalytic activityi

UDP-alpha-D-glucuronate + [protein]-3-O-(beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl)-L-serine = UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi208ManganeseBy similarity1
Active sitei294Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: GO_Central
  • chondroitin sulfate biosynthetic process Source: WormBase
  • embryo development ending in birth or egg hatching Source: WormBase
  • morphogenesis of an epithelium Source: WormBase
  • oviposition Source: WormBase
  • pharynx development Source: WormBase
  • reproduction Source: WormBase
  • spermatid development Source: WormBase
  • vulval development Source: WormBase

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-CEL-1971475. A tetrasaccharide linker sequence is required for GAG synthesis.

Protein family/group databases

CAZyiGT43. Glycosyltransferase Family 43.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase sqv-81 Publication (EC:2.4.1.1351 Publication)
Alternative name(s):
Glucuronyl transferase I1 Publication
Short name:
GlcAT-I1 Publication
Squashed vulva protein 8
Vulval invagination protein sqv-8
Gene namesi
Name:sqv-8
ORF Names:ZK1307.5
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiZK1307.5; CE01694; WBGene00005026; sqv-8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicSequence analysis9
Transmembranei10 – 30Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini31 – 356LumenalSequence analysisAdd BLAST326

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: WormBase

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

F1 adults accumulate eggs in the uterus. Embryos are predominantly arrested at the bean/comma embryonic stage.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi200G → D in n2825; embryos are arrested at the bean/comma or 2/3-fold embryonic stages. Pharyngeal isthmus is shorter and thicker. 2 Publications1
Mutagenesisi205G → E in n2847; embryos are predominantly arrested at the bean/comma embryonic stage. 2 Publications1
Mutagenesisi226G → E in n2843; embryos are predominantly arrested at the bean/comma embryonic stage. 2 Publications1
Mutagenesisi265D → N in n2851; embryos are predominantly arrested at the bean/comma embryonic stage. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001951781 – 356Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase sqv-8Add BLAST356

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi93N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi173N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi246N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi272N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ09363.
PaxDbiQ09363.
PeptideAtlasiQ09363.
PRIDEiQ09363.

PTM databases

iPTMnetiQ09363.

Expressioni

Gene expression databases

BgeeiWBGene00005026.

Interactioni

Protein-protein interaction databases

STRINGi6239.ZK1307.5.

Structurei

3D structure databases

ProteinModelPortaliQ09363.
SMRiQ09363.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 43 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1476. Eukaryota.
ENOG410XP79. LUCA.
GeneTreeiENSGT00390000017640.
HOGENOMiHOG000261693.
InParanoidiQ09363.
KOiK10158.
OMAiFHTAWEP.
OrthoDBiEOG091G0G8P.
PhylomeDBiQ09363.

Family and domain databases

CDDicd00218. GlcAT-I. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiView protein in InterPro
IPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
PANTHERiPTHR10896. PTHR10896. 1 hit.
PfamiView protein in Pfam
PF03360. Glyco_transf_43. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q09363-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFPSRLLEKW WLRAFIALVI FFVWQLFYAI NRVQSLEEER ATLQATIEVL
60 70 80 90 100
TRKSDGLRTQ IFEKERNLVR LNGKVEEIDT QIRDHLSLLP RVNRSTPFIY
110 120 130 140 150
FITPTHFRAA QRADLTRLSY TLSHVPNLHW IVVEDSDELT PSIAGILKRS
160 170 180 190 200
KIPNTHLNAR TPSDQKMRYD DPNWTLPRGV EQRNRALLWI QNQLSGVKEG
210 220 230 240 250
VVYFGDDDNT YDLKIFGEMR KVKNAGVWPV GIVGGMFVET PILEKNGSIS
260 270 280 290 300
HFNAVWKPER PFPIDMAAFA VNISLVLSNA NALFSFDVPR GYQESTFLEN
310 320 330 340 350
LGIHRYNMEP LAEMCTKVYV WHTRTEKPKL SKESIDRLTK KTGFNSLEAH

ALGVDN
Length:356
Mass (Da):41,089
Last modified:November 1, 1997 - v1
Checksum:iB1342554590251A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005864 mRNA. Translation: CAA06741.1.
Z47358 Genomic DNA. Translation: CAA87436.1.
PIRiT27733.
RefSeqiNP_496076.1. NM_063675.4.
UniGeneiCel.18243.

Genome annotation databases

EnsemblMetazoaiZK1307.5; ZK1307.5; WBGene00005026.
GeneIDi174517.
KEGGicel:CELE_ZK1307.5.
UCSCiZK1307.5. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005864 mRNA. Translation: CAA06741.1.
Z47358 Genomic DNA. Translation: CAA87436.1.
PIRiT27733.
RefSeqiNP_496076.1. NM_063675.4.
UniGeneiCel.18243.

3D structure databases

ProteinModelPortaliQ09363.
SMRiQ09363.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.ZK1307.5.

Protein family/group databases

CAZyiGT43. Glycosyltransferase Family 43.

PTM databases

iPTMnetiQ09363.

Proteomic databases

EPDiQ09363.
PaxDbiQ09363.
PeptideAtlasiQ09363.
PRIDEiQ09363.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiZK1307.5; ZK1307.5; WBGene00005026.
GeneIDi174517.
KEGGicel:CELE_ZK1307.5.
UCSCiZK1307.5. c. elegans.

Organism-specific databases

CTDi174517.
WormBaseiZK1307.5; CE01694; WBGene00005026; sqv-8.

Phylogenomic databases

eggNOGiKOG1476. Eukaryota.
ENOG410XP79. LUCA.
GeneTreeiENSGT00390000017640.
HOGENOMiHOG000261693.
InParanoidiQ09363.
KOiK10158.
OMAiFHTAWEP.
OrthoDBiEOG091G0G8P.
PhylomeDBiQ09363.

Enzyme and pathway databases

ReactomeiR-CEL-1971475. A tetrasaccharide linker sequence is required for GAG synthesis.

Miscellaneous databases

PROiPR:Q09363.

Gene expression databases

BgeeiWBGene00005026.

Family and domain databases

CDDicd00218. GlcAT-I. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiView protein in InterPro
IPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
PANTHERiPTHR10896. PTHR10896. 1 hit.
PfamiView protein in Pfam
PF03360. Glyco_transf_43. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSQV8_CAEEL
AccessioniPrimary (citable) accession number: Q09363
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 7, 2017
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.