Reviewed, UniProtKB/Swiss-Prot Q09363 (SQV8_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable glucuronosyltransferase sqv-8
    EC=2.4.1.135
Alternative name(s):
    Vulval invagination protein sqv-8
    Squashed vulva protein 8

Gene names

Name:	sqv-8
ORF Names:	ZK1307.5

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in the biosynthesis of the HNK-1 carbohydrate epitope on glycoproteins. Also involved in wild-type vulval invagination.
Catalytic activity	UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.
Subcellular location	Membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 43 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal-anchor Transmembrane
Ligand	Manganese Metal-binding
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	growth Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Probable glucuronosyltransferase sqv-8		PRO_0000195178
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 30	21	Signal-anchor for type II membrane protein Potential
	Topological domain	31 – 356	326	Lumenal Potential
Sites
	Active site	294	1	Proton acceptor By similarity
	Metal binding	208	1	Manganese By similarity
Amino acid modifications
	Glycosylation	93	1	N-linked (GlcNAc...) Potential
	Glycosylation	173	1	N-linked (GlcNAc...) Potential
	Glycosylation	246	1	N-linked (GlcNAc...) Potential
	Glycosylation	272	1	N-linked (GlcNAc...) Potential
Experimental info
	Mutagenesis	200	1	G → D in N2825. Ref.1
	Mutagenesis	205	1	G → E in N2847. Ref.1
	Mutagenesis	226	1	G → E in N2843. Ref.1
	Mutagenesis	246	1	N → I in N2822. Ref.1
	Mutagenesis	265	1	D → N in N2851. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q09363-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: B1342554590251A9	FASTA	356	41,089
	10 20 30 40 50 60 MFPSRLLEKW WLRAFIALVI FFVWQLFYAI NRVQSLEEER ATLQATIEVL TRKSDGLRTQ 70 80 90 100 110 120 IFEKERNLVR LNGKVEEIDT QIRDHLSLLP RVNRSTPFIY FITPTHFRAA QRADLTRLSY 130 140 150 160 170 180 TLSHVPNLHW IVVEDSDELT PSIAGILKRS KIPNTHLNAR TPSDQKMRYD DPNWTLPRGV 190 200 210 220 230 240 EQRNRALLWI QNQLSGVKEG VVYFGDDDNT YDLKIFGEMR KVKNAGVWPV GIVGGMFVET 250 260 270 280 290 300 PILEKNGSIS HFNAVWKPER PFPIDMAAFA VNISLVLSNA NALFSFDVPR GYQESTFLEN 310 320 330 340 350 LGIHRYNMEP LAEMCTKVYV WHTRTEKPKL SKESIDRLTK KTGFNSLEAH ALGVDN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Three proteins involved in Caenorhabditis elegans vulval invagination are similar to components of a glycosylation pathway." Herman T., Horvitz H.R. Proc. Natl. Acad. Sci. U.S.A. 96:974-979(1999) [PubMed: 9927678] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF GLY-200; GLY-205; GLY-226; ASN-246 AND ASP-265. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ005864 mRNA. Translation: CAA06741.1. Z47358 Genomic DNA. Translation: CAA87436.1.
PIR	T27733.
RefSeq	NP_496076.1.
UniGene	Cel.18243
3D structure databases
HSSP	HSSP built from PDB template 1KWS based on UniProtKB O94766.
ModBase	Search...
Protein family/group databases
CAZy	GT43. Glycosyltransferase Family 43.
Genome annotation databases
Ensembl	ZK1307.5. Caenorhabditis elegans. [Contig view]
GeneID	174517.
KEGG	cel:ZK1307.5.
NMPDR	fig|6239.3.peg.6984.
Organism-specific databases
WormBase	WBGene00005026. sqv-8.
WormPep	ZK1307.5. CE01694. [WorfDB]
Phylogenomic databases
OMA	Q09363. REGEPGW.
Enzyme and pathway databases
BRENDA	2.4.1.135. 672.
Gene expression databases
ArrayExpress	Q09363.
Family and domain databases
InterPro	IPR005027. Glyco_trans_43. [Graphical view]
PANTHER	PTHR10896. Glyco_trans_43. 1 hit.
Pfam	PF03360. Glyco_transf_43. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	884364.

Entry information

Entry name

SQV8_CAEEL

Accession

Primary (citable) accession number: Q09363

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project