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Q09363 (SQV8_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable glucuronosyltransferase sqv-8
EC=2.4.1.135
Alternative name(s):
Squashed vulva protein 8
Vulval invagination protein sqv-8
Gene names
Name:sqv-8
ORF Names:ZK1307.5
OrganismCaenorhabditis elegans
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in the biosynthesis of the HNK-1 carbohydrate epitope on glycoproteins. Also involved in wild-type vulval invagination.

Catalytic activity

UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.

Subcellular location

Membrane; Single-pass type II membrane protein Potential.

Sequence similarities

Belongs to the glycosyltransferase 43 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 356356Probable glucuronosyltransferase sqv-8
PRO_0000195178

Regions

Topological domain1 – 99Cytoplasmic Potential
Transmembrane10 – 3021Helical; Signal-anchor for type II membrane protein; Potential
Topological domain31 – 356326Lumenal Potential

Sites

Active site2941Proton acceptor By similarity
Metal binding2081Manganese By similarity

Amino acid modifications

Glycosylation931N-linked (GlcNAc...) Potential
Glycosylation1731N-linked (GlcNAc...) Potential
Glycosylation2461N-linked (GlcNAc...) Potential
Glycosylation2721N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis2001G → D in N2825. Ref.1
Mutagenesis2051G → E in N2847. Ref.1
Mutagenesis2261G → E in N2843. Ref.1
Mutagenesis2461N → I in N2822. Ref.1
Mutagenesis2651D → N in N2851. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q09363 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: B1342554590251A9

FASTA35641,089
        10         20         30         40         50         60 
MFPSRLLEKW WLRAFIALVI FFVWQLFYAI NRVQSLEEER ATLQATIEVL TRKSDGLRTQ 

        70         80         90        100        110        120 
IFEKERNLVR LNGKVEEIDT QIRDHLSLLP RVNRSTPFIY FITPTHFRAA QRADLTRLSY 

       130        140        150        160        170        180 
TLSHVPNLHW IVVEDSDELT PSIAGILKRS KIPNTHLNAR TPSDQKMRYD DPNWTLPRGV 

       190        200        210        220        230        240 
EQRNRALLWI QNQLSGVKEG VVYFGDDDNT YDLKIFGEMR KVKNAGVWPV GIVGGMFVET 

       250        260        270        280        290        300 
PILEKNGSIS HFNAVWKPER PFPIDMAAFA VNISLVLSNA NALFSFDVPR GYQESTFLEN 

       310        320        330        340        350 
LGIHRYNMEP LAEMCTKVYV WHTRTEKPKL SKESIDRLTK KTGFNSLEAH ALGVDN

« Hide

References

« Hide 'large scale' references
[1]"Three proteins involved in Caenorhabditis elegans vulval invagination are similar to components of a glycosylation pathway."
Herman T., Horvitz H.R.
Proc. Natl. Acad. Sci. U.S.A. 96:974-979(1999) [PubMed: 9927678] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF GLY-200; GLY-205; GLY-226; ASN-246 AND ASP-265.
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ005864 mRNA. Translation: CAA06741.1.
Z47358 Genomic DNA. Translation: CAA87436.1.
PIRT27733.
RefSeqNP_496076.1. NM_063675.3.
UniGeneCel.18243.

3D structure databases

ProteinModelPortalQ09363.
SMRQ09363. Positions 96-348.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ09363.

Protein family/group databases

CAZyGT43. Glycosyltransferase Family 43.

Proteomic databases

PRIDEQ09363.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaZK1307.5; ZK1307.5; ZK1307.5.
GeneID174517.
KEGGcel:ZK1307.5.
NMPDRfig|6239.3.peg.6984.
UCSCZK1307.5. c. elegans.

Organism-specific databases

CTD174517.
WormBaseZK1307.5; CE01694; WBGene00005026; sqv-8.

Phylogenomic databases

eggNOGmeNOG08148.
GeneTreeEMGT00050000013702.
HOGENOMHBG443614.
InParanoidQ09363.
OMAADESEME.
PhylomeDBQ09363.

Gene expression databases

ArrayExpressQ09363.

Family and domain databases

InterProIPR005027. Glyco_trans_43.
[Graphical view]
KOK10158.
PANTHERPTHR10896. Glyco_trans_43. 1 hit.
PfamPF03360. Glyco_transf_43. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio884364.

Entry information

Entry nameSQV8_CAEEL
AccessionPrimary (citable) accession number: Q09363
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 16, 2011
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormPep

SIMILARITY comments

Index of protein domains and families

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