Reviewed, UniProtKB/Swiss-Prot Q09332 (UGGG_DROME)

Names and origin

Protein names

Recommended name:
    UDP-glucose:glycoprotein glucosyltransferase
    EC=2.4.1.-
Alternative name(s):
    UDP--Glc:glycoprotein glucosyltransferase
    dUGT

Gene names

Name:	Ugt
Synonyms:	UGGG
ORF Names:	CG6850

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recognizes glycoproteins with minor folding defects. Reglucosylates single N-glycans near the misfolded part of the protein, thus providing quality control for protein folding in the endoplasmic reticulum. Reglucosylated proteins are recognized by calreticulin for recycling to the endoplasmic reticulum and refolding or degradation.
Cofactor	Calcium or manganese.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Monomer.
Subcellular location	Endoplasmic reticulum lumen.
Developmental stage	Is present at low but detectable levels in the earliest embryos, increasing at 6-8 hours with a maximum at 10-12 hours. Levels decrease thereafter and are not detected in 18-20 hours embryos and first instar larvae but is detected again at second instar to pupation.
Sequence similarities	Belongs to the glycosyltransferase 8 family.
Sequence caution	The sequence AAL13582.1 differs from that shown. Reason: Frameshift at position 1060.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell nuclear envelope Inferred from direct assay. Source: FlyBase rough endoplasmic reticulum Inferred from direct assay. Source: FlyBase
Molecular function	UDP-glucose:glycoprotein glucosyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Ref.1
	Chain	23 – 1548	1526	UDP-glucose:glycoprotein glucosyltransferase		PRO_0000012270
Regions
	Region	1227 – 1548	322	Glucosyltransferase By similarity
	Motif	1545 – 1548	4	Prevents secretion from ER Potential
Amino acid modifications
	Glycosylation	181	1	N-linked (GlcNAc...) Potential
	Glycosylation	266	1	N-linked (GlcNAc...) Potential
	Glycosylation	864	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	166	1	S → P in AAA85850. Ref.1
	Sequence conflict	622	1	G → E in AAA85850. Ref.1
	Sequence conflict	748	1	A → T in AAA85850. Ref.1
	Sequence conflict	963	1	N → K in AAA85850. Ref.1
	Sequence conflict	1233	1	T → S in AAA85850. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q09332-1 [UniParc]. Last modified June 7, 2005. Version 2. Checksum: 87D782E7B27E0EE9	FASTA	1,548	174,354
	10 20 30 40 50 60 MLRAVALCVS VVLIALYTPT SGESSQSYPI TTLINAKWTQ TPLYLEIAEY LADEQAGLFW 70 80 90 100 110 120 DYVSGVTKLD TVLNEYDTES QQYNAALELV KSHVSSPQLP LLRLVVSMHS LTPRIQTHFQ 130 140 150 160 170 180 LAEELRSSGS CQSFTFAQVG SELACSFNEL QKKLEVPLAK DSLDASVVTY SFDHIFPGSE 190 200 210 220 230 240 NNTRTVVLYG DLGSSQFRTY HKLLEKEANA GRIRYILRHQ LAKKDKRPVR LSGYGVELHL 250 260 270 280 290 300 KSTEYKSQDD APKPEAGSTS DEDLANESDV QGFDFKVLKQ KHPTLKRALD QLRQRLLQGN 310 320 330 340 350 360 DEIAQLKAWE FQDLGLQAAA AIAEIQGDET LQILQYTAHN FPMLARTLLA HKVTDGLRAE 370 380 390 400 410 420 VKHNTEAFGR SLNVAPPDGA LFINGLFFDA DTMDLYSLIE TLRSEMRVLE SLHSNNVRGS 430 440 450 460 470 480 LASSLLALDL TASSKKEFAI DIRDTAVQWV NDIENDVQYR RWPSSVMDLL RPTFPGMLRN 490 500 510 520 530 540 IRKNVFNLVL VVDALQPTAR SVIKLSESFV IHQAPIRLGL VFDARDANED NLADYVAITC 550 560 570 580 590 600 AYNYVSQKKD ARAALSFLTD IYAAVGETKV VTKKDIVKQL TKEFTSLSFA KAEEFLEEDS 610 620 630 640 650 660 TYDYGRELAA EFIQRLGFGD KGQPQALLNG VPMPSNVVTA DSDFEEAIFT EIMTHTSNLQ 670 680 690 700 710 720 KAVYKGELTD NDVAIDYLMN QPHVMPRLNQ RILSQEDVKY LDINGVAYKN LGNVGVLNRL 730 740 750 760 770 780 SNRDMTATLM DNLKYFGGKK STELIGRASL QFLTIWVFAD LETDQGRDLL THALDYVQSG 790 800 810 820 830 840 ESVRVAFIPN TESSSASSRR NLNRLVWAAM QSLPPTQATE QVLKWLKKPK EKIEIPTQLE 850 860 870 880 890 900 DILGSTELHL KMLRVYSQRV LGLNKSQRLV IGNGRLYGPL SSDESFDSAD FALLARFSSL 910 920 930 940 950 960 QYSDKVRQVL KESAQDVNEE FNSDTLLKLY ASLLPRQTKT RFKLPTDLKT DHSVVKLPPK 970 980 990 1000 1010 1020 QENLPHFDVA AVLDPASRAA QKLTPILILL RQVLNCQLNL YLIPVPQHSD MPVKNFYRYV 1030 1040 1050 1060 1070 1080 VEPEVQFEAN GGRSDGPLAK FSGLPANPLL TQQLQVPENW LVEAVRAVYD LDNIKLTDIG 1090 1100 1110 1120 1130 1140 GPVHSEFDLE YLLLEGHCFD AASGAPPRGL QLVLGTQSQP TLVDTIVMAN LGYFQLKANP 1150 1160 1170 1180 1190 1200 GAWSLRLREG KSADIYAISH IEGTNTHHSA GSSEVQVLIT SLRSHVVKLR VSKKPGMQQA 1210 1220 1230 1240 1250 1260 ELLSDDNEQA AQSGMWNSIA SSFGGGSANQ AATDEDTETI NIFSVASGHL YERLLRIMMV 1270 1280 1290 1300 1310 1320 SLLKHTKSPV KFWFLKNYLS PQFTDFLPHM ASEYNFQYEL VQYKWPRWLH QQTEKQRTIW 1330 1340 1350 1360 1370 1380 GYKILFLDVL FPLNVRKIIF VDADAIVRTD IKELYDMDLG GAPYAYTPFC DSRKEMEGFR 1390 1400 1410 1420 1430 1440 FWKQGYWRSH LMGRRYHISA LYVVDLKRFR KIAAGDRLRG QYQALSQDPN SLSNLDQDLP 1450 1460 1470 1480 1490 1500 NNMIHQVAIK SLPDDWLWCQ TWCSDSNFKT AKVIDLCNNP QTKEAKLTAA QRIVPEWKDY 1510 1520 1530 1540 DAELKTLMSR IEDHENSHSR DSAVDDSVDD SVEVTTVTPS HEPKHGEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Drosophila UDP-glucose:glycoprotein glucosyltransferase: sequence and characterization of an enzyme that distinguishes between denatured and native proteins." Parker C.G., Fessler L.I., Nelson R.E., Fessler J.H. EMBO J. 14:1294-1303(1995) [PubMed: 7729408] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 23-37. Tissue: Embryo.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Head.
[5]	Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U20554 mRNA. Translation: AAA85850.1. AE014296 Genomic DNA. Translation: AAF49220.1. AY058353 mRNA. Translation: AAL13582.1. Frameshift. BT044158 mRNA. Translation: ACH92223.1.
PIR	S54723.
RefSeq	NP_524151.2.
UniGene	Dm.7749
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q09332. 3 interactions.
Protein family/group databases
CAZy	GT24. Glycosyltransferase Family 24.
Genome annotation databases
Ensembl	FBgn0014075. Drosophila melanogaster. [Contig view]
GeneID	40055.
KEGG	dme:Dmel_CG6850.
NMPDR	fig|7227.3.peg.10469.
Organism-specific databases
FlyBase	FBgn0014075. Ugt.
Phylogenomic databases
HOGENOM	Q09332.
OMA	Q09332. GDHRYPS.
Gene expression databases
ArrayExpress	Q09332.
GermOnline	CG6850. Drosophila melanogaster.
Family and domain databases
InterPro	IPR009448. UDP-g_GGtrans. [Graphical view]
PANTHER	PTHR11226. UDP-g_GGtrans. 1 hit.
Pfam	PF06427. UDP-g_GGTase. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	816774.

Entry information

Entry name

UGGG_DROME

Accession

Primary (citable) accession number: Q09332
Secondary accession number(s): B5RIN4, Q95U28, Q9VVT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 7, 2005
Last modified:	June 16, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project