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Reviewed, UniProtKB/Swiss-Prot Q09328 (MGT5A_HUMAN)

Last modified June 16, 2009. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
    EC=2.4.1.155
Alternative name(s):
    Mannoside acetylglucosaminyltransferase 5
    Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase
    N-acetylglucosaminyl-transferase V
    GlcNAc-T V
      Short name=GNT-V
Gene names
Name: MGAT5
Synonyms: GGNT5
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length741 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.

Catalytic activity

UDP-N-acetyl-D-glucosamine + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein.

Sequence similarities

Belongs to the glycosyltransferase 18 family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 741741Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
PRO_0000080522

Regions

Topological domain1 – 1313Cytoplasmic Potential
Transmembrane14 – 3017Signal-anchor for type II membrane protein Potential
Topological domain31 – 741711Lumenal Potential

Amino acid modifications

Glycosylation1101N-linked (GlcNAc...) Potential
Glycosylation1151N-linked (GlcNAc...) Potential
Glycosylation1181N-linked (GlcNAc...) Potential
Glycosylation3341N-linked (GlcNAc...) Potential
Glycosylation4331N-linked (GlcNAc...) Potential
Glycosylation4471N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
Q09328-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 1073F8E67AD0E23B

FASTA74184,543
        10         20         30         40         50         60 
MALFTPWKLS SQKLGFFLVT FGFIWGMMLL HFTIQQRTQP ESSSMLREQI LDLSKRYIKA 

        70         80         90        100        110        120 
LAEENRNVVD GPYAGVMTAY DLKKTLAVLL DNILQRIGKL ESKVDNLVVN GTGTNSTNST 

       130        140        150        160        170        180 
TAVPSLVALE KINVADIING AQEKCVLPPM DGYPHCEGKI KWMKDMWRSD PCYADYGVDG 

       190        200        210        220        230        240 
STCSFFIYLS EVENWCPHLP WRAKNPYEEA DHNSLAEIRT DFNILYSMMK KHEEFRWMRL 

       250        260        270        280        290        300 
RIRRMADAWI QAIKSLAEKQ NLEKRKRKKV LVHLGLLTKE SGFKIAETAF SGGPLGELVQ 

       310        320        330        340        350        360 
WSDLITSLYL LGHDIRISAS LAELKEIMKK VVGNRSGCPT VGDRIVELIY IDIVGLAQFK 

       370        380        390        400        410        420 
KTLGPSWVHY QCMLRVLDSF GTEPEFNHAN YAQSKGHKTP WGKWNLNPQQ FYTMFPHTPD 

       430        440        450        460        470        480 
NSFLGFVVEQ HLNSSDIHHI NEIKRQNQSL VYGKVDSFWK NKKIYLDIIH TYMEVHATVY 

       490        500        510        520        530        540 
GSSTKNIPSY VKNHGILSGR DLQFLLRETK LFVGLGFPYE GPAPLEAIAN GCAFLNPKFN 

       550        560        570        580        590        600 
PPKSSKNTDF FIGKPTLREL TSQHPYAEVF IGRPHVWTVD LNNQEEVEDA VKAILNQKIE 

       610        620        630        640        650        660 
PYMPYEFTCE GMLQRINAFI EKQDFCHGQV MWPPLSALQV KLAEPGQSCK QVCQESQLIC 

       670        680        690        700        710        720 
EPSFFQHLNK DKDMLKYKVT CQSSELAKDI LVPSFDPKNK HCVFQGDLLL FSCAGAHPRH 

       730        740 
QRVCPCRDFI KGQVALCKDC L

« Hide

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References

[1]"cDNA cloning and chromosomal mapping of human N-acetylglucosaminyltransferase V+."
Nishikawa A., Saito H., Gu J., Ihara Y., Soejima H., Wada Y., Sekiya C., Kangawa K., Niikawa N., Taniguchi N.
Biochem. Biophys. Res. Commun. 198:318-327(1994) [PubMed: 8292036] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[2]"Characterization of UDP-N-acetylglucosamine: alpha-6-d-mannoside beta-1,6-N-acetylglucosaminyltransferase V from a human hepatoma cell line Hep3B."
Park C., Jin U.H., Lee Y.C., Cho T.J., Kim C.H.
Arch. Biochem. Biophys. 367:281-288(1999) [PubMed: 10395745] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

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Web resources

GGDB

GlycoGene database

Functional Glycomics Gateway - GTase

Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferaseV

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Cross-references

Sequence databases

D17716 mRNA. Translation: BAA04570.1.
AF113921 mRNA. Translation: AAD22449.1.
IPIIPI00020407.
PIRJC2074.
RefSeqNP_002401.1.
UniGeneHs.651869

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGT18. Glycosyltransferase Family 18.

PTM databases

PhosphoSiteQ09328.

Proteomic databases

PeptideAtlasQ09328.
PRIDEQ09328.

Genome annotation databases

EnsemblENSG00000152127. Homo sapiens. [Contig view]
GeneID4249.
KEGGhsa:4249.

Organism-specific databases

GeneCardsGC02P134594.
H-InvDBHIX0006523.
HIX0029814.
HGNCHGNC:7049. MGAT5.
MIM601774. gene.
PharmGKBPA30784.
GenAtlasSearch...

Phylogenomic databases

HOGENOMQ09328.
HOVERGENQ09328.
OMAQ09328. ENQLICE.

Enzyme and pathway databases

BRENDA2.4.1.155. 247.

Gene expression databases

ArrayExpressQ09328.
BgeeQ09328.
CleanExHS_MGAT5.
GermOnlineENSG00000152127. Homo sapiens.

Family and domain databases

ProtoNetSearch...

Other Resources

NextBio16751.
SOURCESearch...

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Entry information

Entry nameMGT5A_HUMAN
AccessionPrimary (citable) accession number: Q09328
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 16, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 2

Human chromosome 2: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents