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Protein

Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A

Gene

MGAT5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:HS07793-MONOMER.
ZFISH:HS07793-MONOMER.
BRENDAi2.4.1.155. 2681.
ReactomeiR-HSA-975577. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT18. Glycosyltransferase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (EC:2.4.1.155)
Alternative name(s):
Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase
GlcNAc-T V
Short name:
GNT-V
Mannoside acetylglucosaminyltransferase 5
N-acetylglucosaminyl-transferase V
Gene namesi
Name:MGAT5
Synonyms:GGNT5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:7049. MGAT5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 13CytoplasmicSequence analysisAdd BLAST13
Transmembranei14 – 30Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini31 – 741LumenalSequence analysisAdd BLAST711

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • Golgi membrane Source: Reactome
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi4249.
OpenTargetsiENSG00000152127.
PharmGKBiPA30784.

Chemistry databases

ChEMBLiCHEMBL1795131.

Polymorphism and mutation databases

BioMutaiMGAT5.
DMDMi1169980.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805221 – 741Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase AAdd BLAST741

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi110N-linked (GlcNAc...)Sequence analysis1
Glycosylationi115N-linked (GlcNAc...)Sequence analysis1
Glycosylationi118N-linked (GlcNAc...)Sequence analysis1
Glycosylationi334N-linked (GlcNAc...)Sequence analysis1
Glycosylationi433N-linked (GlcNAc...)Sequence analysis1
Glycosylationi447N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ09328.
MaxQBiQ09328.
PaxDbiQ09328.
PeptideAtlasiQ09328.
PRIDEiQ09328.

PTM databases

iPTMnetiQ09328.
PhosphoSitePlusiQ09328.

Expressioni

Gene expression databases

BgeeiENSG00000152127.
CleanExiHS_MGAT5.
GenevisibleiQ09328. HS.

Organism-specific databases

HPAiHPA010919.

Interactioni

Protein-protein interaction databases

BioGridi110405. 16 interactors.
IntActiQ09328. 1 interactor.
STRINGi9606.ENSP00000281923.

Structurei

3D structure databases

ProteinModelPortaliQ09328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 18 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IDYS. Eukaryota.
ENOG410XTV7. LUCA.
GeneTreeiENSGT00390000012263.
HOGENOMiHOG000006557.
HOVERGENiHBG052469.
InParanoidiQ09328.
KOiK00744.
OMAiGKVDNFW.
OrthoDBiEOG091G044F.
PhylomeDBiQ09328.
TreeFamiTF313714.

Family and domain databases

InterProiIPR027833. DUF4525.
IPR026116. GlyclTrfase_18.
[Graphical view]
PANTHERiPTHR15075. PTHR15075. 1 hit.
PfamiPF15027. DUF4525. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09328-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALFTPWKLS SQKLGFFLVT FGFIWGMMLL HFTIQQRTQP ESSSMLREQI
60 70 80 90 100
LDLSKRYIKA LAEENRNVVD GPYAGVMTAY DLKKTLAVLL DNILQRIGKL
110 120 130 140 150
ESKVDNLVVN GTGTNSTNST TAVPSLVALE KINVADIING AQEKCVLPPM
160 170 180 190 200
DGYPHCEGKI KWMKDMWRSD PCYADYGVDG STCSFFIYLS EVENWCPHLP
210 220 230 240 250
WRAKNPYEEA DHNSLAEIRT DFNILYSMMK KHEEFRWMRL RIRRMADAWI
260 270 280 290 300
QAIKSLAEKQ NLEKRKRKKV LVHLGLLTKE SGFKIAETAF SGGPLGELVQ
310 320 330 340 350
WSDLITSLYL LGHDIRISAS LAELKEIMKK VVGNRSGCPT VGDRIVELIY
360 370 380 390 400
IDIVGLAQFK KTLGPSWVHY QCMLRVLDSF GTEPEFNHAN YAQSKGHKTP
410 420 430 440 450
WGKWNLNPQQ FYTMFPHTPD NSFLGFVVEQ HLNSSDIHHI NEIKRQNQSL
460 470 480 490 500
VYGKVDSFWK NKKIYLDIIH TYMEVHATVY GSSTKNIPSY VKNHGILSGR
510 520 530 540 550
DLQFLLRETK LFVGLGFPYE GPAPLEAIAN GCAFLNPKFN PPKSSKNTDF
560 570 580 590 600
FIGKPTLREL TSQHPYAEVF IGRPHVWTVD LNNQEEVEDA VKAILNQKIE
610 620 630 640 650
PYMPYEFTCE GMLQRINAFI EKQDFCHGQV MWPPLSALQV KLAEPGQSCK
660 670 680 690 700
QVCQESQLIC EPSFFQHLNK DKDMLKYKVT CQSSELAKDI LVPSFDPKNK
710 720 730 740
HCVFQGDLLL FSCAGAHPRH QRVCPCRDFI KGQVALCKDC L
Length:741
Mass (Da):84,543
Last modified:November 1, 1995 - v1
Checksum:i1073F8E67AD0E23B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17716 mRNA. Translation: BAA04570.1.
AF113921 mRNA. Translation: AAD22449.1.
CH471058 Genomic DNA. Translation: EAX11657.1.
CH471058 Genomic DNA. Translation: EAX11658.1.
CCDSiCCDS2171.1.
PIRiJC2074.
RefSeqiNP_002401.1. NM_002410.4.
XP_005263725.1. XM_005263668.4.
XP_005263726.1. XM_005263669.4.
XP_005263727.1. XM_005263670.3.
XP_006712597.1. XM_006712534.3.
XP_011509501.1. XM_011511199.2.
XP_011509502.1. XM_011511200.1.
XP_011509503.1. XM_011511201.2.
XP_011509504.1. XM_011511202.1.
XP_016859636.1. XM_017004147.1.
XP_016859637.1. XM_017004148.1.
UniGeneiHs.4988.

Genome annotation databases

EnsembliENST00000281923; ENSP00000281923; ENSG00000152127.
ENST00000409645; ENSP00000386377; ENSG00000152127.
GeneIDi4249.
KEGGihsa:4249.
UCSCiuc002ttw.5. human.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferaseV

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17716 mRNA. Translation: BAA04570.1.
AF113921 mRNA. Translation: AAD22449.1.
CH471058 Genomic DNA. Translation: EAX11657.1.
CH471058 Genomic DNA. Translation: EAX11658.1.
CCDSiCCDS2171.1.
PIRiJC2074.
RefSeqiNP_002401.1. NM_002410.4.
XP_005263725.1. XM_005263668.4.
XP_005263726.1. XM_005263669.4.
XP_005263727.1. XM_005263670.3.
XP_006712597.1. XM_006712534.3.
XP_011509501.1. XM_011511199.2.
XP_011509502.1. XM_011511200.1.
XP_011509503.1. XM_011511201.2.
XP_011509504.1. XM_011511202.1.
XP_016859636.1. XM_017004147.1.
XP_016859637.1. XM_017004148.1.
UniGeneiHs.4988.

3D structure databases

ProteinModelPortaliQ09328.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110405. 16 interactors.
IntActiQ09328. 1 interactor.
STRINGi9606.ENSP00000281923.

Chemistry databases

ChEMBLiCHEMBL1795131.

Protein family/group databases

CAZyiGT18. Glycosyltransferase Family 18.

PTM databases

iPTMnetiQ09328.
PhosphoSitePlusiQ09328.

Polymorphism and mutation databases

BioMutaiMGAT5.
DMDMi1169980.

Proteomic databases

EPDiQ09328.
MaxQBiQ09328.
PaxDbiQ09328.
PeptideAtlasiQ09328.
PRIDEiQ09328.

Protocols and materials databases

DNASUi4249.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000281923; ENSP00000281923; ENSG00000152127.
ENST00000409645; ENSP00000386377; ENSG00000152127.
GeneIDi4249.
KEGGihsa:4249.
UCSCiuc002ttw.5. human.

Organism-specific databases

CTDi4249.
DisGeNETi4249.
GeneCardsiMGAT5.
HGNCiHGNC:7049. MGAT5.
HPAiHPA010919.
MIMi601774. gene.
neXtProtiNX_Q09328.
OpenTargetsiENSG00000152127.
PharmGKBiPA30784.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IDYS. Eukaryota.
ENOG410XTV7. LUCA.
GeneTreeiENSGT00390000012263.
HOGENOMiHOG000006557.
HOVERGENiHBG052469.
InParanoidiQ09328.
KOiK00744.
OMAiGKVDNFW.
OrthoDBiEOG091G044F.
PhylomeDBiQ09328.
TreeFamiTF313714.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciMetaCyc:HS07793-MONOMER.
ZFISH:HS07793-MONOMER.
BRENDAi2.4.1.155. 2681.
ReactomeiR-HSA-975577. N-Glycan antennae elongation.

Miscellaneous databases

ChiTaRSiMGAT5. human.
GeneWikiiMGAT5.
GenomeRNAii4249.
PROiQ09328.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000152127.
CleanExiHS_MGAT5.
GenevisibleiQ09328. HS.

Family and domain databases

InterProiIPR027833. DUF4525.
IPR026116. GlyclTrfase_18.
[Graphical view]
PANTHERiPTHR15075. PTHR15075. 1 hit.
PfamiPF15027. DUF4525. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMGT5A_HUMAN
AccessioniPrimary (citable) accession number: Q09328
Secondary accession number(s): D3DP70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.