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Protein

Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

Gene

MGAT3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

It is involved in the regulation of the biosynthesis and biological function of glycoprotein oligosaccharides. Catalyzes the addition of N-acetylglucosamine in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-linked sugar chains. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-(beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6))-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-(beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6))-(beta-D-GlcNAc-(1->4))-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein].

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:HS05168-MONOMER
ReactomeiR-HSA-975574 Reactions specific to the hybrid N-glycan synthesis pathway
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT17 Glycosyltransferase Family 17

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase (EC:2.4.1.144)
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III
Short name:
GNT-III
Short name:
GlcNAc-T III
Short name:
N-acetylglucosaminyltransferase III
Gene namesi
Name:MGAT3
Synonyms:GGNT3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000128268.11
HGNCiHGNC:7046 MGAT3
MIMi604621 gene
neXtProtiNX_Q09327

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 23Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST16
Topological domaini24 – 533LumenalSequence analysisAdd BLAST510

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi4248
OpenTargetsiENSG00000128268
PharmGKBiPA30781

Chemistry databases

ChEMBLiCHEMBL2375206

Polymorphism and mutation databases

BioMutaiMGAT3
DMDMi61252497

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001888421 – 533Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferaseAdd BLAST533

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi141N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi241N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi259N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi397N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ09327
PeptideAtlasiQ09327
PRIDEiQ09327

PTM databases

iPTMnetiQ09327
PhosphoSitePlusiQ09327

Expressioni

Gene expression databases

BgeeiENSG00000128268
CleanExiHS_MGAT3
ExpressionAtlasiQ09327 baseline and differential
GenevisibleiQ09327 HS

Organism-specific databases

HPAiHPA017598

Interactioni

Subunit structurei

Interacts with MGAT4D.By similarity

Protein-protein interaction databases

BioGridi110404, 1 interactor
STRINGi9606.ENSP00000345270

Chemistry databases

BindingDBiQ09327

Structurei

3D structure databases

ProteinModelPortaliQ09327
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi35 – 86Pro-richAdd BLAST52

Sequence similaritiesi

Belongs to the glycosyltransferase 17 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEQJ Eukaryota
ENOG410YQKP LUCA
GeneTreeiENSGT00390000008221
HOGENOMiHOG000113579
HOVERGENiHBG052468
InParanoidiQ09327
KOiK00737
OMAiMKMRRHK
OrthoDBiEOG091G0CDF
PhylomeDBiQ09327
TreeFamiTF323781

Family and domain databases

InterProiView protein in InterPro
IPR006813 Glyco_trans_17
PANTHERiPTHR12224 PTHR12224, 1 hit
PfamiView protein in Pfam
PF04724 Glyco_transf_17, 1 hit

Sequencei

Sequence statusi: Complete.

Q09327-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMRRYKLFL MFCMAGLCLI SFLHFFKTLS YVTFPRELAS LSPNLVSSFF
60 70 80 90 100
WNNAPVTPQA SPEPGGPDLL RTPLYSHSPL LQPLPPSKAA EELHRVDLVL
110 120 130 140 150
PEDTTEYFVR TKAGGVCFKP GTKMLERPPP GRPEEKPEGA NGSSARRPPR
160 170 180 190 200
YLLSARERTG GRGARRKWVE CVCLPGWHGP SCGVPTVVQY SNLPTKERLV
210 220 230 240 250
PREVPRRVIN AINVNHEFDL LDVRFHELGD VVDAFVVCES NFTAYGEPRP
260 270 280 290 300
LKFREMLTNG TFEYIRHKVL YVFLDHFPPG GRQDGWIADD YLRTFLTQDG
310 320 330 340 350
VSRLRNLRPD DVFIIDDADE IPARDGVLFL KLYDGWTEPF AFHMRKSLYG
360 370 380 390 400
FFWKQPGTLE VVSGCTVDML QAVYGLDGIR LRRRQYYTMP NFRQYENRTG
410 420 430 440 450
HILVQWSLGS PLHFAGWHCS WCFTPEGIYF KLVSAQNGDF PRWGDYEDKR
460 470 480 490 500
DLNYIRGLIR TGGWFDGTQQ EYPPADPSEH MYAPKYLLKN YDRFHYLLDN
510 520 530
PYQEPRSTAA GGWRHRGPEG RPPARGKLDE AEV
Length:533
Mass (Da):61,313
Last modified:March 15, 2005 - v3
Checksum:i11F56C44A6B173AB
GO

Sequence cautioni

The sequence BAA02937 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti346K → T in BAA02937 (PubMed:8370666).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13789 mRNA Translation: BAA02937.1 Different initiation.
CR456519 mRNA Translation: CAG30405.1
AL022312 Genomic DNA No translation available.
BC075025 mRNA Translation: AAH75025.1
BC075026 mRNA Translation: AAH75026.1
BC113383 mRNA Translation: AAI13384.1
BC113718 mRNA Translation: AAI13719.1
CCDSiCCDS13994.2
PIRiJN0586
RefSeqiNP_001091740.1, NM_001098270.1
NP_002400.3, NM_002409.4
XP_005261666.1, XM_005261609.2
XP_011528486.1, XM_011530184.2
XP_011528487.1, XM_011530185.2
XP_011528488.1, XM_011530186.2
XP_011528489.1, XM_011530187.2
XP_011528490.1, XM_011530188.2
XP_011528491.1, XM_011530189.2
XP_011528492.1, XM_011530190.2
XP_011528493.1, XM_011530191.2
XP_011528494.1, XM_011530192.2
XP_011528496.1, XM_011530194.2
XP_016884291.1, XM_017028802.1
UniGeneiHs.276808

Genome annotation databases

EnsembliENST00000341184; ENSP00000345270; ENSG00000128268
GeneIDi4248
KEGGihsa:4248
UCSCiuc003axv.6 human

Similar proteinsi

Entry informationi

Entry nameiMGAT3_HUMAN
AccessioniPrimary (citable) accession number: Q09327
Secondary accession number(s): A6NGD0
, Q14CK5, Q6IC49, Q9UH32
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: March 15, 2005
Last modified: March 28, 2018
This is version 150 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health