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Q09326

- MGAT2_RAT

UniProt

Q09326 - MGAT2_RAT

Protein

Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

Gene

Mgat2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalyzes an essential step in the conversion of oligo-mannose and hybrid to complex N-glycans.

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + 6-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

    Pathwayi

    GO - Molecular functioni

    1. alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Source: RGD
    2. carbohydrate binding Source: RGD

    GO - Biological processi

    1. oligosaccharide biosynthetic process Source: InterPro
    2. protein N-linked glycosylation Source: RGD
    3. protein N-linked glycosylation via asparagine Source: Ensembl

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.1.143. 5301.
    ReactomeiREACT_199013. Reactions specific to the complex N-glycan synthesis pathway.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT16. Glycosyltransferase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC:2.4.1.143)
    Alternative name(s):
    Beta-1,2-N-acetylglucosaminyltransferase II
    GlcNAc-T II
    Short name:
    GNT-II
    Mannoside acetylglucosaminyltransferase 2
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II
    Gene namesi
    Name:Mgat2
    Synonyms:Gnt2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 6

    Organism-specific databases

    RGDi620098. Mgat2.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. Golgi stack Source: InterPro
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 442442Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferasePRO_0000080520Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi64 – 641N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi81 – 811N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ09326.
    PRIDEiQ09326.

    Expressioni

    Gene expression databases

    GenevestigatoriQ09326.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4565815.
    STRINGi10116.ENSRNOP00000005608.

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 99CytoplasmicSequence Analysis
    Topological domaini30 – 442413LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei10 – 2920Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG239633.
    GeneTreeiENSGT00390000007341.
    HOGENOMiHOG000261661.
    HOVERGENiHBG052467.
    InParanoidiQ09326.
    KOiK00736.
    OMAiHAGDCGM.
    OrthoDBiEOG7TF79W.
    PhylomeDBiQ09326.
    TreeFamiTF314772.

    Family and domain databases

    Gene3Di3.90.550.10. 2 hits.
    InterProiIPR007754. GlcNAc_II.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR12871. PTHR12871. 1 hit.
    PfamiPF05060. MGAT2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q09326-1 [UniParc]FASTAAdd to Basket

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    MRFRIYKRKV LILTLVVAAC GFVLWSSNGR QRKNDALAPP LLDSEPLRGA    50
    GHFAASVGIR RVSNDSAAPL VPAVPRPEVD NLTLRYRSLV YQLNFDQMLR 100
    NVDKDGTWSP GELVLVVQVH NRPEYLRLLI DSLRKAQGIR EVLVIFSHDF 150
    WSAEINSLIS SVDFCPVLQV FFPFSIQLYP SEFPGSDPRD CPRDLKKNAA 200
    LKLGCINAEY PDSFGHYREA KFSQTKHHWW WKLHFVWERV KVLQDYTGLI 250
    LFLEEDHYLA PDFYHVFKKM WKLKQQECPG CDVLSLGTYT TIRSFYGIAD 300
    KVDVKTWKST EHNMGLALTR DAYQKLIECT DTFCTYDDYN WDWTLQYLTL 350
    ACLPKVWKVL VPQAPRIFHA GDCGMHHKKT CRPSTQSAQI ESLLNNNKQY 400
    LFPETLVIGE KFPMAAISPP RKNGGWGDIR DHELCKSYRR LQ 442
    Length:442
    Mass (Da):51,110
    Last modified:November 1, 1995 - v1
    Checksum:i86A9C8210AA8B7C4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U21662 mRNA. Translation: AAA86721.1.
    BC081754 mRNA. Translation: AAH81754.1.
    PIRiA57044.
    RefSeqiNP_446056.1. NM_053604.2.
    UniGeneiRn.2342.

    Genome annotation databases

    EnsembliENSRNOT00000005608; ENSRNOP00000005608; ENSRNOG00000004234.
    GeneIDi94273.
    KEGGirno:94273.
    UCSCiRGD:620098. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U21662 mRNA. Translation: AAA86721.1 .
    BC081754 mRNA. Translation: AAH81754.1 .
    PIRi A57044.
    RefSeqi NP_446056.1. NM_053604.2.
    UniGenei Rn.2342.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4565815.
    STRINGi 10116.ENSRNOP00000005608.

    Protein family/group databases

    CAZyi GT16. Glycosyltransferase Family 16.

    Proteomic databases

    PaxDbi Q09326.
    PRIDEi Q09326.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000005608 ; ENSRNOP00000005608 ; ENSRNOG00000004234 .
    GeneIDi 94273.
    KEGGi rno:94273.
    UCSCi RGD:620098. rat.

    Organism-specific databases

    CTDi 4247.
    RGDi 620098. Mgat2.

    Phylogenomic databases

    eggNOGi NOG239633.
    GeneTreei ENSGT00390000007341.
    HOGENOMi HOG000261661.
    HOVERGENi HBG052467.
    InParanoidi Q09326.
    KOi K00736.
    OMAi HAGDCGM.
    OrthoDBi EOG7TF79W.
    PhylomeDBi Q09326.
    TreeFami TF314772.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BRENDAi 2.4.1.143. 5301.
    Reactomei REACT_199013. Reactions specific to the complex N-glycan synthesis pathway.

    Miscellaneous databases

    NextBioi 617917.
    PROi Q09326.

    Gene expression databases

    Genevestigatori Q09326.

    Family and domain databases

    Gene3Di 3.90.550.10. 2 hits.
    InterProi IPR007754. GlcNAc_II.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR12871. PTHR12871. 1 hit.
    Pfami PF05060. MGAT2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of cDNA encoding the rat UDP-N-acetylglucosamine:alpha-6-D-mannoside beta-1,2-N-acetylglucosaminyltransferase II."
      D'Agostaro G.A.F., Zingoni A., Moritz R.L., Simpson R.J., Schachter H., Bendiak B.
      J. Biol. Chem. 270:15211-15221(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Strain: Sprague-Dawley.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiMGAT2_RAT
    AccessioniPrimary (citable) accession number: Q09326
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways

    External Data

    Dasty 3