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Protein

Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

Gene

Mgat2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes an essential step in the conversion of oligo-mannose and hybrid to complex N-glycans.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 6-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Pathway: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Source: RGD
  • carbohydrate binding Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.143. 5301.
ReactomeiREACT_343829. Reactions specific to the complex N-glycan synthesis pathway.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT16. Glycosyltransferase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC:2.4.1.143)
Alternative name(s):
Beta-1,2-N-acetylglucosaminyltransferase II
GlcNAc-T II
Short name:
GNT-II
Mannoside acetylglucosaminyltransferase 2
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II
Gene namesi
Name:Mgat2
Synonyms:Gnt2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi620098. Mgat2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicSequence Analysis
Transmembranei10 – 2920Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini30 – 442413LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 442442Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferasePRO_0000080520Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi64 – 641N-linked (GlcNAc...)Sequence Analysis
Glycosylationi81 – 811N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ09326.
PRIDEiQ09326.

Expressioni

Gene expression databases

GenevisibleiQ09326. RN.

Interactioni

Protein-protein interaction databases

MINTiMINT-4565815.
STRINGi10116.ENSRNOP00000005608.

Family & Domainsi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG239633.
GeneTreeiENSGT00390000007341.
HOGENOMiHOG000261661.
HOVERGENiHBG052467.
InParanoidiQ09326.
KOiK00736.
OMAiASVDFCQ.
OrthoDBiEOG7TF79W.
PhylomeDBiQ09326.
TreeFamiTF314772.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
InterProiIPR007754. GlcNAc_II.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR12871. PTHR12871. 1 hit.
PfamiPF05060. MGAT2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

Q09326-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFRIYKRKV LILTLVVAAC GFVLWSSNGR QRKNDALAPP LLDSEPLRGA
60 70 80 90 100
GHFAASVGIR RVSNDSAAPL VPAVPRPEVD NLTLRYRSLV YQLNFDQMLR
110 120 130 140 150
NVDKDGTWSP GELVLVVQVH NRPEYLRLLI DSLRKAQGIR EVLVIFSHDF
160 170 180 190 200
WSAEINSLIS SVDFCPVLQV FFPFSIQLYP SEFPGSDPRD CPRDLKKNAA
210 220 230 240 250
LKLGCINAEY PDSFGHYREA KFSQTKHHWW WKLHFVWERV KVLQDYTGLI
260 270 280 290 300
LFLEEDHYLA PDFYHVFKKM WKLKQQECPG CDVLSLGTYT TIRSFYGIAD
310 320 330 340 350
KVDVKTWKST EHNMGLALTR DAYQKLIECT DTFCTYDDYN WDWTLQYLTL
360 370 380 390 400
ACLPKVWKVL VPQAPRIFHA GDCGMHHKKT CRPSTQSAQI ESLLNNNKQY
410 420 430 440
LFPETLVIGE KFPMAAISPP RKNGGWGDIR DHELCKSYRR LQ
Length:442
Mass (Da):51,110
Last modified:November 1, 1995 - v1
Checksum:i86A9C8210AA8B7C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21662 mRNA. Translation: AAA86721.1.
BC081754 mRNA. Translation: AAH81754.1.
PIRiA57044.
RefSeqiNP_446056.1. NM_053604.2.
UniGeneiRn.2342.

Genome annotation databases

EnsembliENSRNOT00000005608; ENSRNOP00000005608; ENSRNOG00000004234.
GeneIDi94273.
KEGGirno:94273.
UCSCiRGD:620098. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U21662 mRNA. Translation: AAA86721.1.
BC081754 mRNA. Translation: AAH81754.1.
PIRiA57044.
RefSeqiNP_446056.1. NM_053604.2.
UniGeneiRn.2342.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4565815.
STRINGi10116.ENSRNOP00000005608.

Protein family/group databases

CAZyiGT16. Glycosyltransferase Family 16.

Proteomic databases

PaxDbiQ09326.
PRIDEiQ09326.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000005608; ENSRNOP00000005608; ENSRNOG00000004234.
GeneIDi94273.
KEGGirno:94273.
UCSCiRGD:620098. rat.

Organism-specific databases

CTDi4247.
RGDi620098. Mgat2.

Phylogenomic databases

eggNOGiNOG239633.
GeneTreeiENSGT00390000007341.
HOGENOMiHOG000261661.
HOVERGENiHBG052467.
InParanoidiQ09326.
KOiK00736.
OMAiASVDFCQ.
OrthoDBiEOG7TF79W.
PhylomeDBiQ09326.
TreeFamiTF314772.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.1.143. 5301.
ReactomeiREACT_343829. Reactions specific to the complex N-glycan synthesis pathway.

Miscellaneous databases

NextBioi617917.
PROiQ09326.

Gene expression databases

GenevisibleiQ09326. RN.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
InterProiIPR007754. GlcNAc_II.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR12871. PTHR12871. 1 hit.
PfamiPF05060. MGAT2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of cDNA encoding the rat UDP-N-acetylglucosamine:alpha-6-D-mannoside beta-1,2-N-acetylglucosaminyltransferase II."
    D'Agostaro G.A.F., Zingoni A., Moritz R.L., Simpson R.J., Schachter H., Bendiak B.
    J. Biol. Chem. 270:15211-15221(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiMGAT2_RAT
AccessioniPrimary (citable) accession number: Q09326
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 24, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.