Reviewed, UniProtKB/Swiss-Prot Q09326 (MGAT2_RAT)

Names and origin

Protein names

Recommended name:
    Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
    EC=2.4.1.143
Alternative name(s):
    Mannoside acetylglucosaminyltransferase 2
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II
    Beta-1,2-N-acetylglucosaminyltransferase II
    GlcNAc-T II
      Short name=GNT-II

Gene names

Name:	Mgat2
Synonyms:	Gnt2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes an essential step in the conversion of oligo-mannose and hybrid to complex N-glycans.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 6-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oligosaccharide biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi stack Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Inferred from direct assay. Source: RGD sugar binding Inferred from physical interaction. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase		PRO_0000080520
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 29	20	Signal-anchor for type II membrane protein Potential
	Topological domain	30 – 442	413	Lumenal Potential
Amino acid modifications
	Glycosylation	64	1	N-linked (GlcNAc...) Potential
	Glycosylation	81	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q09326-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 86A9C8210AA8B7C4	FASTA	442	51,110
	10 20 30 40 50 60 MRFRIYKRKV LILTLVVAAC GFVLWSSNGR QRKNDALAPP LLDSEPLRGA GHFAASVGIR 70 80 90 100 110 120 RVSNDSAAPL VPAVPRPEVD NLTLRYRSLV YQLNFDQMLR NVDKDGTWSP GELVLVVQVH 130 140 150 160 170 180 NRPEYLRLLI DSLRKAQGIR EVLVIFSHDF WSAEINSLIS SVDFCPVLQV FFPFSIQLYP 190 200 210 220 230 240 SEFPGSDPRD CPRDLKKNAA LKLGCINAEY PDSFGHYREA KFSQTKHHWW WKLHFVWERV 250 260 270 280 290 300 KVLQDYTGLI LFLEEDHYLA PDFYHVFKKM WKLKQQECPG CDVLSLGTYT TIRSFYGIAD 310 320 330 340 350 360 KVDVKTWKST EHNMGLALTR DAYQKLIECT DTFCTYDDYN WDWTLQYLTL ACLPKVWKVL 370 380 390 400 410 420 VPQAPRIFHA GDCGMHHKKT CRPSTQSAQI ESLLNNNKQY LFPETLVIGE KFPMAAISPP 430 440 RKNGGWGDIR DHELCKSYRR LQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of cDNA encoding the rat UDP-N-acetylglucosamine:alpha-6-D-mannoside beta-1,2-N-acetylglucosaminyltransferase II." D'Agostaro G.A.F., Zingoni A., Moritz R.L., Simpson R.J., Schachter H., Bendiak B. J. Biol. Chem. 270:15211-15221(1995) [PubMed: 7797505] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U21662 mRNA. Translation: AAA86721.1. BC081754 mRNA. Translation: AAH81754.1.
IPI	IPI00203419.
PIR	A57044.
RefSeq	NP_446056.1.
UniGene	Rn.2342
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q09326.
Protein family/group databases
CAZy	GT16. Glycosyltransferase Family 16.
Proteomic databases
PRIDE	Q09326.
Genome annotation databases
Ensembl	ENSRNOT00000005608; ENSRNOP00000005608; ENSRNOG00000004234; Rattus norvegicus. [Genome view]
GeneID	94273.
KEGG	rno:94273.
UCSC	NM_053604. rat.
Organism-specific databases
CTD	94273.
RGD	620098. Mgat2.
Phylogenomic databases
eggNOG	roNOG13800.
HOVERGEN	Q09326.
InParanoid	Q09326.
OMA	KVDVKTW.
OrthoDB	EOG9QVFXZ.
PhylomeDB	Q09326.
Enzyme and pathway databases
BRENDA	2.4.1.143. 248.
Gene expression databases
ArrayExpress	Q09326.
Genevestigator	Q09326.
GermOnline	ENSRNOG00000004234. Rattus norvegicus.
Family and domain databases
InterPro	IPR007754. GlcNAc_II. [Graphical view]
PANTHER	PTHR12871. MGAT2. 1 hit.
Pfam	PF05060. MGAT2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	617917.

Entry information

Entry name

MGAT2_RAT

Accession

Primary (citable) accession number: Q09326

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 19, 2010
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)