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Q09326 (MGAT2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

EC=2.4.1.143
Alternative name(s):
Beta-1,2-N-acetylglucosaminyltransferase II
GlcNAc-T II
Short name=GNT-II
Mannoside acetylglucosaminyltransferase 2
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II
Gene names
Name:Mgat2
Synonyms:Gnt2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length442 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes an essential step in the conversion of oligo-mannose and hybrid to complex N-glycans.

Catalytic activity

UDP-N-acetyl-D-glucosamine + 6-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 442442Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
PRO_0000080520

Regions

Topological domain1 – 99Cytoplasmic Potential
Transmembrane10 – 2920Helical; Signal-anchor for type II membrane protein; Potential
Topological domain30 – 442413Lumenal Potential

Amino acid modifications

Glycosylation641N-linked (GlcNAc...) Potential
Glycosylation811N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q09326 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 86A9C8210AA8B7C4

FASTA44251,110
        10         20         30         40         50         60 
MRFRIYKRKV LILTLVVAAC GFVLWSSNGR QRKNDALAPP LLDSEPLRGA GHFAASVGIR 

        70         80         90        100        110        120 
RVSNDSAAPL VPAVPRPEVD NLTLRYRSLV YQLNFDQMLR NVDKDGTWSP GELVLVVQVH 

       130        140        150        160        170        180 
NRPEYLRLLI DSLRKAQGIR EVLVIFSHDF WSAEINSLIS SVDFCPVLQV FFPFSIQLYP 

       190        200        210        220        230        240 
SEFPGSDPRD CPRDLKKNAA LKLGCINAEY PDSFGHYREA KFSQTKHHWW WKLHFVWERV 

       250        260        270        280        290        300 
KVLQDYTGLI LFLEEDHYLA PDFYHVFKKM WKLKQQECPG CDVLSLGTYT TIRSFYGIAD 

       310        320        330        340        350        360 
KVDVKTWKST EHNMGLALTR DAYQKLIECT DTFCTYDDYN WDWTLQYLTL ACLPKVWKVL 

       370        380        390        400        410        420 
VPQAPRIFHA GDCGMHHKKT CRPSTQSAQI ESLLNNNKQY LFPETLVIGE KFPMAAISPP 

       430        440 
RKNGGWGDIR DHELCKSYRR LQ 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and expression of cDNA encoding the rat UDP-N-acetylglucosamine:alpha-6-D-mannoside beta-1,2-N-acetylglucosaminyltransferase II."
D'Agostaro G.A.F., Zingoni A., Moritz R.L., Simpson R.J., Schachter H., Bendiak B.
J. Biol. Chem. 270:15211-15221(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: Sprague-Dawley.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U21662 mRNA. Translation: AAA86721.1.
BC081754 mRNA. Translation: AAH81754.1.
PIRA57044.
RefSeqNP_446056.1. NM_053604.2.
UniGeneRn.2342.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4565815.
STRING10116.ENSRNOP00000005608.

Protein family/group databases

CAZyGT16. Glycosyltransferase Family 16.

Proteomic databases

PaxDbQ09326.
PRIDEQ09326.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000005608; ENSRNOP00000005608; ENSRNOG00000004234.
GeneID94273.
KEGGrno:94273.
UCSCRGD:620098. rat.

Organism-specific databases

CTD4247.
RGD620098. Mgat2.

Phylogenomic databases

eggNOGNOG239633.
GeneTreeENSGT00390000007341.
HOGENOMHOG000261661.
HOVERGENHBG052467.
InParanoidQ09326.
KOK00736.
OMAHAGDCGM.
OrthoDBEOG7TF79W.
PhylomeDBQ09326.
TreeFamTF314772.

Enzyme and pathway databases

BRENDA2.4.1.143. 5301.
UniPathwayUPA00378.

Gene expression databases

GenevestigatorQ09326.

Family and domain databases

Gene3D3.90.550.10. 2 hits.
InterProIPR007754. GlcNAc_II.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERPTHR12871. PTHR12871. 1 hit.
PfamPF05060. MGAT2. 1 hit.
[Graphical view]
SUPFAMSSF53448. SSF53448. 2 hits.
ProtoNetSearch...

Other

NextBio617917.
PROQ09326.

Entry information

Entry nameMGAT2_RAT
AccessionPrimary (citable) accession number: Q09326
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways