Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

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Q09326 (MGAT2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 98. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
EC=2.4.1.143

Alternative name(s):
Beta-1,2-N-acetylglucosaminyltransferase II
GlcNAc-T II
Short name=GNT-II
Mannoside acetylglucosaminyltransferase 2
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II

Gene names

Name:	Mgat2
Synonyms:	Gnt2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes an essential step in the conversion of oligo-mannose and hybrid to complex N-glycans.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 6-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	oligosaccharide biosynthetic process Inferred from electronic annotation. Source: InterPro protein N-linked glycosylation Inferred from direct assay PubMed 2952645. Source: RGD protein N-linked glycosylation via asparagine Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi stack Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Inferred from direct assay PubMed 2952645. Source: RGD carbohydrate binding Inferred from physical interaction PubMed 2952645. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 442

442

Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

PRO_0000080520

Regions

Topological domain

1 – 9

Cytoplasmic Potential

Transmembrane

10 – 29

Helical; Signal-anchor for type II membrane protein; Potential

Topological domain

30 – 442

413

Lumenal Potential

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Glycosylation

N-linked (GlcNAc...) Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q09326 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 86A9C8210AA8B7C4
Show »

FASTA

442

51,110

        10         20         30         40         50         60 
MRFRIYKRKV LILTLVVAAC GFVLWSSNGR QRKNDALAPP LLDSEPLRGA GHFAASVGIR 

        70         80         90        100        110        120 
RVSNDSAAPL VPAVPRPEVD NLTLRYRSLV YQLNFDQMLR NVDKDGTWSP GELVLVVQVH 

       130        140        150        160        170        180 
NRPEYLRLLI DSLRKAQGIR EVLVIFSHDF WSAEINSLIS SVDFCPVLQV FFPFSIQLYP 

       190        200        210        220        230        240 
SEFPGSDPRD CPRDLKKNAA LKLGCINAEY PDSFGHYREA KFSQTKHHWW WKLHFVWERV 

       250        260        270        280        290        300 
KVLQDYTGLI LFLEEDHYLA PDFYHVFKKM WKLKQQECPG CDVLSLGTYT TIRSFYGIAD 

       310        320        330        340        350        360 
KVDVKTWKST EHNMGLALTR DAYQKLIECT DTFCTYDDYN WDWTLQYLTL ACLPKVWKVL 

       370        380        390        400        410        420 
VPQAPRIFHA GDCGMHHKKT CRPSTQSAQI ESLLNNNKQY LFPETLVIGE KFPMAAISPP 

       430        440 
RKNGGWGDIR DHELCKSYRR LQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of cDNA encoding the rat UDP-N-acetylglucosamine:alpha-6-D-mannoside beta-1,2-N-acetylglucosaminyltransferase II." D'Agostaro G.A.F., Zingoni A., Moritz R.L., Simpson R.J., Schachter H., Bendiak B. J. Biol. Chem. 270:15211-15221(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U21662 mRNA. Translation: AAA86721.1. BC081754 mRNA. Translation: AAH81754.1.
IPI	IPI00203419.
PIR	A57044.
RefSeq	NP_446056.1. NM_053604.2.
UniGene	Rn.2342.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4565815.
STRING	10116.ENSRNOP00000005608.
Protein family/group databases
CAZy	GT16. Glycosyltransferase Family 16.
Proteomic databases
PaxDb	Q09326.
PRIDE	Q09326.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000005608; ENSRNOP00000005608; ENSRNOG00000004234.
GeneID	94273.
KEGG	rno:94273.
UCSC	RGD:620098. rat.
Organism-specific databases
CTD	4247.
RGD	620098. Mgat2.
Phylogenomic databases
eggNOG	NOG239633.
GeneTree	ENSGT00390000007341.
HOGENOM	HOG000261661.
HOVERGEN	HBG052467.
InParanoid	Q09326.
KO	K00736.
OMA	HAGDCGM.
OrthoDB	EOG4VX255.
Enzyme and pathway databases
BRENDA	2.4.1.143. 5301.
UniPathway	UPA00378.
Gene expression databases
Genevestigator	Q09326.
GermOnline	ENSRNOG00000004234. Rattus norvegicus.
Family and domain databases
InterPro	IPR007754. GlcNAc_II. [Graphical view]
PANTHER	PTHR12871. PTHR12871. 1 hit.
Pfam	PF05060. MGAT2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	617917.

Entry information

Entry name

MGAT2_RAT

Accession

Primary (citable) accession number: Q09326

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 29, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents