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Q09326

- MGAT2_RAT

UniProt

Q09326 - MGAT2_RAT

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Protein

Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

Gene

Mgat2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes an essential step in the conversion of oligo-mannose and hybrid to complex N-glycans.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 6-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Pathwayi

GO - Molecular functioni

  1. alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Source: RGD
  2. carbohydrate binding Source: RGD

GO - Biological processi

  1. oligosaccharide biosynthetic process Source: InterPro
  2. protein N-linked glycosylation Source: RGD
  3. protein N-linked glycosylation via asparagine Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.143. 5301.
ReactomeiREACT_199013. Reactions specific to the complex N-glycan synthesis pathway.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT16. Glycosyltransferase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC:2.4.1.143)
Alternative name(s):
Beta-1,2-N-acetylglucosaminyltransferase II
GlcNAc-T II
Short name:
GNT-II
Mannoside acetylglucosaminyltransferase 2
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II
Gene namesi
Name:Mgat2
Synonyms:Gnt2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 6

Organism-specific databases

RGDi620098. Mgat2.

Subcellular locationi

GO - Cellular componenti

  1. Golgi stack Source: InterPro
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 442442Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferasePRO_0000080520Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi64 – 641N-linked (GlcNAc...)Sequence Analysis
Glycosylationi81 – 811N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ09326.
PRIDEiQ09326.

Expressioni

Gene expression databases

GenevestigatoriQ09326.

Interactioni

Protein-protein interaction databases

MINTiMINT-4565815.
STRINGi10116.ENSRNOP00000005608.

Structurei

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicSequence Analysis
Topological domaini30 – 442413LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei10 – 2920Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG239633.
GeneTreeiENSGT00390000007341.
HOGENOMiHOG000261661.
HOVERGENiHBG052467.
InParanoidiQ09326.
KOiK00736.
OMAiHAGDCGM.
OrthoDBiEOG7TF79W.
PhylomeDBiQ09326.
TreeFamiTF314772.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
InterProiIPR007754. GlcNAc_II.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR12871. PTHR12871. 1 hit.
PfamiPF05060. MGAT2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

Q09326-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRFRIYKRKV LILTLVVAAC GFVLWSSNGR QRKNDALAPP LLDSEPLRGA
60 70 80 90 100
GHFAASVGIR RVSNDSAAPL VPAVPRPEVD NLTLRYRSLV YQLNFDQMLR
110 120 130 140 150
NVDKDGTWSP GELVLVVQVH NRPEYLRLLI DSLRKAQGIR EVLVIFSHDF
160 170 180 190 200
WSAEINSLIS SVDFCPVLQV FFPFSIQLYP SEFPGSDPRD CPRDLKKNAA
210 220 230 240 250
LKLGCINAEY PDSFGHYREA KFSQTKHHWW WKLHFVWERV KVLQDYTGLI
260 270 280 290 300
LFLEEDHYLA PDFYHVFKKM WKLKQQECPG CDVLSLGTYT TIRSFYGIAD
310 320 330 340 350
KVDVKTWKST EHNMGLALTR DAYQKLIECT DTFCTYDDYN WDWTLQYLTL
360 370 380 390 400
ACLPKVWKVL VPQAPRIFHA GDCGMHHKKT CRPSTQSAQI ESLLNNNKQY
410 420 430 440
LFPETLVIGE KFPMAAISPP RKNGGWGDIR DHELCKSYRR LQ
Length:442
Mass (Da):51,110
Last modified:November 1, 1995 - v1
Checksum:i86A9C8210AA8B7C4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U21662 mRNA. Translation: AAA86721.1.
BC081754 mRNA. Translation: AAH81754.1.
PIRiA57044.
RefSeqiNP_446056.1. NM_053604.2.
UniGeneiRn.2342.

Genome annotation databases

EnsembliENSRNOT00000005608; ENSRNOP00000005608; ENSRNOG00000004234.
GeneIDi94273.
KEGGirno:94273.
UCSCiRGD:620098. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U21662 mRNA. Translation: AAA86721.1 .
BC081754 mRNA. Translation: AAH81754.1 .
PIRi A57044.
RefSeqi NP_446056.1. NM_053604.2.
UniGenei Rn.2342.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4565815.
STRINGi 10116.ENSRNOP00000005608.

Protein family/group databases

CAZyi GT16. Glycosyltransferase Family 16.

Proteomic databases

PaxDbi Q09326.
PRIDEi Q09326.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000005608 ; ENSRNOP00000005608 ; ENSRNOG00000004234 .
GeneIDi 94273.
KEGGi rno:94273.
UCSCi RGD:620098. rat.

Organism-specific databases

CTDi 4247.
RGDi 620098. Mgat2.

Phylogenomic databases

eggNOGi NOG239633.
GeneTreei ENSGT00390000007341.
HOGENOMi HOG000261661.
HOVERGENi HBG052467.
InParanoidi Q09326.
KOi K00736.
OMAi HAGDCGM.
OrthoDBi EOG7TF79W.
PhylomeDBi Q09326.
TreeFami TF314772.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BRENDAi 2.4.1.143. 5301.
Reactomei REACT_199013. Reactions specific to the complex N-glycan synthesis pathway.

Miscellaneous databases

NextBioi 617917.
PROi Q09326.

Gene expression databases

Genevestigatori Q09326.

Family and domain databases

Gene3Di 3.90.550.10. 2 hits.
InterProi IPR007754. GlcNAc_II.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR12871. PTHR12871. 1 hit.
Pfami PF05060. MGAT2. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of cDNA encoding the rat UDP-N-acetylglucosamine:alpha-6-D-mannoside beta-1,2-N-acetylglucosaminyltransferase II."
    D'Agostaro G.A.F., Zingoni A., Moritz R.L., Simpson R.J., Schachter H., Bendiak B.
    J. Biol. Chem. 270:15211-15221(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiMGAT2_RAT
AccessioniPrimary (citable) accession number: Q09326
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways

External Data

Dasty 3