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Q09325 (MGAT1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

EC=2.4.1.101
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
Short name=GNT-I
Short name=GlcNAc-T I
Gene names
Name:Mgat1
Synonyms:Gnt1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length447 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.

Catalytic activity

UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Cofactor

Manganese. The cofactor is mostly bound to the substrate By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein.

Tissue specificity

Appears to be present in all tissues.

Sequence similarities

Belongs to the glycosyltransferase 13 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 447447Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
PRO_0000191386

Regions

Topological domain1 – 66Cytoplasmic Potential
Transmembrane7 – 2923Helical; Signal-anchor for type II membrane protein; Potential
Topological domain30 – 447418Lumenal Potential

Sites

Active site2911Proton acceptor Potential
Metal binding2131Manganese By similarity
Binding site1171Substrate By similarity
Binding site1441Substrate By similarity
Binding site1901Substrate By similarity
Binding site2121Substrate By similarity
Binding site3221Substrate By similarity

Amino acid modifications

Disulfide bond115 ↔ 145 By similarity
Disulfide bond239 ↔ 305 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q09325 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 1AB54107072C53A2

FASTA44751,586
        10         20         30         40         50         60 
MLKKQSAGLV LWGAIIFVGW NALLLLFFWT RPAPGRLPSD SALGDDPASL TREVIHLAED 

        70         80         90        100        110        120 
AEAELERQRG LLQQIKEHYS LWRQRWRVPT VAPPAWPRVP GTPSPAVIPI LVIACDRSTV 

       130        140        150        160        170        180 
RRCLDKLLHY RPSAEHFPII VSQDCGHEET AQVIASYGTA VTHIRQPDLS NIAVQPDHRK 

       190        200        210        220        230        240 
FQGYYKIARH YRWALGQIFN KFKFPAAVVV EDDLEVAPDF FEYFQATYPL LKADPSLWCV 

       250        260        270        280        290        300 
SAWNDNGKEQ MVDSSKPELL YRTDFFPGLG WLLLADLWAE LEPKWPKAFW DDWMRRPEQR 

       310        320        330        340        350        360 
KGRACIRPEI SRTMTFGRKG VSHGQFFDQH LKFIKLNQQF VPFTQLDLSY LQREAYDRDF 

       370        380        390        400        410        420 
LAQVYGAPQL QVEKVRTNDR KELGEVRVQY TSRDSFKAFA KALGVMDDLK SGVPRAGYRG 

       430        440 
IVTFQFRGRR VHLAPPETWN GYDPSWN 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of a cDNA encoding N-acetylglucosaminyltransferase I from rat liver and analysis of its expression in rat tissues."
Fukada T., Iida K., Kioka N., Sakai H., Komano T.
Biosci. Biotechnol. Biochem. 58:200-201(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Liver.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D16302 mRNA. Translation: BAA03807.1.
BC074010 mRNA. Translation: AAH74010.1.
PIRJC2076.
RefSeqNP_110488.1. NM_030861.1.
XP_006246244.1. XM_006246182.1.
XP_006246245.1. XM_006246183.1.
XP_006246246.1. XM_006246184.1.
XP_006246247.1. XM_006246185.1.
XP_006246248.1. XM_006246186.1.
UniGeneRn.2712.

3D structure databases

ProteinModelPortalQ09325.
SMRQ09325. Positions 106-446.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4565797.
STRING10116.ENSRNOP00000044560.

Protein family/group databases

CAZyGT13. Glycosyltransferase Family 13.

PTM databases

PhosphoSiteQ09325.

Proteomic databases

PaxDbQ09325.
PRIDEQ09325.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000052360; ENSRNOP00000044560; ENSRNOG00000031208.
GeneID81519.
KEGGrno:81519.
UCSCRGD:620097. rat.

Organism-specific databases

CTD4245.
RGD620097. Mgat1.

Phylogenomic databases

eggNOGNOG148227.
GeneTreeENSGT00530000063632.
HOGENOMHOG000247055.
HOVERGENHBG052466.
InParanoidQ09325.
KOK00726.
OMAHEETAQV.
OrthoDBEOG7R56SN.
PhylomeDBQ09325.
TreeFamTF320555.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

GenevestigatorQ09325.

Family and domain databases

InterProIPR004139. Glyco_trans_13.
[Graphical view]
PANTHERPTHR10468. PTHR10468. 1 hit.
PfamPF03071. GNT-I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio615029.
PROQ09325.

Entry information

Entry nameMGAT1_RAT
AccessionPrimary (citable) accession number: Q09325
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways