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Q09325

- MGAT1_RAT

UniProt

Q09325 - MGAT1_RAT

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Protein
Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
Gene
Mgat1, Gnt1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Cofactori

Manganese. The cofactor is mostly bound to the substrate By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei117 – 1171Substrate By similarity
Binding sitei144 – 1441Substrate By similarity
Binding sitei190 – 1901Substrate By similarity
Binding sitei212 – 2121Substrate By similarity
Metal bindingi213 – 2131Manganese By similarity
Active sitei291 – 2911Proton acceptor Reviewed prediction
Binding sitei322 – 3221Substrate By similarity

GO - Molecular functioni

  1. alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. UDP-N-acetylglucosamine catabolic process Source: Ensembl
  2. in utero embryonic development Source: Ensembl
  3. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_199031. N-glycan trimming and elongation in the cis-Golgi.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT13. Glycosyltransferase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC:2.4.1.101)
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
Short name:
GNT-I
Short name:
GlcNAc-T I
Gene namesi
Name:Mgat1
Synonyms:Gnt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi620097. Mgat1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66Cytoplasmic Reviewed prediction
Transmembranei7 – 2923Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini30 – 447418Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
PRO_0000191386Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi115 ↔ 145 By similarity
Disulfide bondi239 ↔ 305 By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ09325.
PRIDEiQ09325.

PTM databases

PhosphoSiteiQ09325.

Expressioni

Tissue specificityi

Appears to be present in all tissues.

Gene expression databases

GenevestigatoriQ09325.

Interactioni

Protein-protein interaction databases

MINTiMINT-4565797.
STRINGi10116.ENSRNOP00000044560.

Structurei

3D structure databases

ProteinModelPortaliQ09325.
SMRiQ09325. Positions 106-446.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG148227.
GeneTreeiENSGT00530000063632.
HOGENOMiHOG000247055.
HOVERGENiHBG052466.
InParanoidiQ09325.
KOiK00726.
OMAiHEETAQV.
OrthoDBiEOG7R56SN.
PhylomeDBiQ09325.
TreeFamiTF320555.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR004139. Glyco_trans_13.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10468. PTHR10468. 1 hit.
PfamiPF03071. GNT-I. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q09325-1 [UniParc]FASTAAdd to Basket

« Hide

MLKKQSAGLV LWGAIIFVGW NALLLLFFWT RPAPGRLPSD SALGDDPASL    50
TREVIHLAED AEAELERQRG LLQQIKEHYS LWRQRWRVPT VAPPAWPRVP 100
GTPSPAVIPI LVIACDRSTV RRCLDKLLHY RPSAEHFPII VSQDCGHEET 150
AQVIASYGTA VTHIRQPDLS NIAVQPDHRK FQGYYKIARH YRWALGQIFN 200
KFKFPAAVVV EDDLEVAPDF FEYFQATYPL LKADPSLWCV SAWNDNGKEQ 250
MVDSSKPELL YRTDFFPGLG WLLLADLWAE LEPKWPKAFW DDWMRRPEQR 300
KGRACIRPEI SRTMTFGRKG VSHGQFFDQH LKFIKLNQQF VPFTQLDLSY 350
LQREAYDRDF LAQVYGAPQL QVEKVRTNDR KELGEVRVQY TSRDSFKAFA 400
KALGVMDDLK SGVPRAGYRG IVTFQFRGRR VHLAPPETWN GYDPSWN 447
Length:447
Mass (Da):51,586
Last modified:November 1, 1995 - v1
Checksum:i1AB54107072C53A2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16302 mRNA. Translation: BAA03807.1.
BC074010 mRNA. Translation: AAH74010.1.
PIRiJC2076.
RefSeqiNP_110488.1. NM_030861.1.
XP_006246244.1. XM_006246182.1.
XP_006246245.1. XM_006246183.1.
XP_006246246.1. XM_006246184.1.
XP_006246247.1. XM_006246185.1.
XP_006246248.1. XM_006246186.1.
UniGeneiRn.2712.

Genome annotation databases

EnsembliENSRNOT00000052360; ENSRNOP00000044560; ENSRNOG00000031208.
GeneIDi81519.
KEGGirno:81519.
UCSCiRGD:620097. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16302 mRNA. Translation: BAA03807.1 .
BC074010 mRNA. Translation: AAH74010.1 .
PIRi JC2076.
RefSeqi NP_110488.1. NM_030861.1.
XP_006246244.1. XM_006246182.1.
XP_006246245.1. XM_006246183.1.
XP_006246246.1. XM_006246184.1.
XP_006246247.1. XM_006246185.1.
XP_006246248.1. XM_006246186.1.
UniGenei Rn.2712.

3D structure databases

ProteinModelPortali Q09325.
SMRi Q09325. Positions 106-446.
ModBasei Search...

Protein-protein interaction databases

MINTi MINT-4565797.
STRINGi 10116.ENSRNOP00000044560.

Protein family/group databases

CAZyi GT13. Glycosyltransferase Family 13.

PTM databases

PhosphoSitei Q09325.

Proteomic databases

PaxDbi Q09325.
PRIDEi Q09325.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000052360 ; ENSRNOP00000044560 ; ENSRNOG00000031208 .
GeneIDi 81519.
KEGGi rno:81519.
UCSCi RGD:620097. rat.

Organism-specific databases

CTDi 4245.
RGDi 620097. Mgat1.

Phylogenomic databases

eggNOGi NOG148227.
GeneTreei ENSGT00530000063632.
HOGENOMi HOG000247055.
HOVERGENi HBG052466.
InParanoidi Q09325.
KOi K00726.
OMAi HEETAQV.
OrthoDBi EOG7R56SN.
PhylomeDBi Q09325.
TreeFami TF320555.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_199031. N-glycan trimming and elongation in the cis-Golgi.

Miscellaneous databases

NextBioi 615029.
PROi Q09325.

Gene expression databases

Genevestigatori Q09325.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR004139. Glyco_trans_13.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR10468. PTHR10468. 1 hit.
Pfami PF03071. GNT-I. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a cDNA encoding N-acetylglucosaminyltransferase I from rat liver and analysis of its expression in rat tissues."
    Fukada T., Iida K., Kioka N., Sakai H., Komano T.
    Biosci. Biotechnol. Biochem. 58:200-201(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiMGAT1_RAT
AccessioniPrimary (citable) accession number: Q09325
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 3, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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