Reviewed, UniProtKB/Swiss-Prot Q09325 (MGAT1_RAT)

Names and origin

Protein names

Recommended name:
    Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
    EC=2.4.1.101
Alternative name(s):
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
      Short name=GlcNAc-T I
      Short name=GNT-I

Gene names

Name:	Mgat1
Synonyms:	Gnt1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Tissue specificity	Appears to be present in all tissues.
Sequence similarities	Belongs to the glycosyltransferase 13 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase		PRO_0000191386
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 29	23	Signal-anchor for type II membrane protein Potential
	Topological domain	30 – 447	418	Lumenal Potential

Sequences

	Sequence		Length	Mass (Da)
	Q09325-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 1AB54107072C53A2	FASTA	447	51,586
	10 20 30 40 50 60 MLKKQSAGLV LWGAIIFVGW NALLLLFFWT RPAPGRLPSD SALGDDPASL TREVIHLAED 70 80 90 100 110 120 AEAELERQRG LLQQIKEHYS LWRQRWRVPT VAPPAWPRVP GTPSPAVIPI LVIACDRSTV 130 140 150 160 170 180 RRCLDKLLHY RPSAEHFPII VSQDCGHEET AQVIASYGTA VTHIRQPDLS NIAVQPDHRK 190 200 210 220 230 240 FQGYYKIARH YRWALGQIFN KFKFPAAVVV EDDLEVAPDF FEYFQATYPL LKADPSLWCV 250 260 270 280 290 300 SAWNDNGKEQ MVDSSKPELL YRTDFFPGLG WLLLADLWAE LEPKWPKAFW DDWMRRPEQR 310 320 330 340 350 360 KGRACIRPEI SRTMTFGRKG VSHGQFFDQH LKFIKLNQQF VPFTQLDLSY LQREAYDRDF 370 380 390 400 410 420 LAQVYGAPQL QVEKVRTNDR KELGEVRVQY TSRDSFKAFA KALGVMDDLK SGVPRAGYRG 430 440 IVTFQFRGRR VHLAPPETWN GYDPSWN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a cDNA encoding N-acetylglucosaminyltransferase I from rat liver and analysis of its expression in rat tissues." Fukada T., Iida K., Kioka N., Sakai H., Komano T. Biosci. Biotechnol. Biochem. 58:200-201(1994) [PubMed: 7764514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D16302 mRNA. Translation: BAA03807.1. BC074010 mRNA. Translation: AAH74010.1.
IPI	IPI00203418.
PIR	JC2076.
RefSeq	NP_110488.1.
UniGene	Rn.2712
3D structure databases
HSSP	HSSP built from PDB template 1FOA based on UniProtKB P27115.
SMR	Q09325. Positions 106-446.
ModBase	Search...
Protein family/group databases
CAZy	GT13. Glycosyltransferase Family 13.
PTM databases
PhosphoSite	Q09325.
Proteomic databases
PRIDE	Q09325.
Genome annotation databases
Ensembl	ENSRNOG00000031208. Rattus norvegicus. [Contig view]
GeneID	81519.
KEGG	rno:81519.
Organism-specific databases
RGD	620097. Mgat1.
Phylogenomic databases
HOVERGEN	Q09325.
OMA	Q09325. PPDHRKF.
Enzyme and pathway databases
BRENDA	2.4.1.101. 248.
Gene expression databases
ArrayExpress	Q09325.
GermOnline	ENSRNOG00000031208. Rattus norvegicus.
Family and domain databases
InterPro	IPR004139. Glyco_trans_13. [Graphical view]
PANTHER	PTHR10468. Glyco_trans_13. 1 hit.
Pfam	PF03071. GNT-I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	615029.

Entry information

Entry name

MGAT1_RAT

Accession

Primary (citable) accession number: Q09325

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)