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Q09325

- MGAT1_RAT

UniProt

Q09325 - MGAT1_RAT

Protein

Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

Gene

Mgat1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

    Cofactori

    Manganese. The cofactor is mostly bound to the substrate By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei117 – 1171SubstrateBy similarity
    Binding sitei144 – 1441SubstrateBy similarity
    Binding sitei190 – 1901SubstrateBy similarity
    Binding sitei212 – 2121SubstrateBy similarity
    Metal bindingi213 – 2131ManganeseBy similarity
    Active sitei291 – 2911Proton acceptorSequence Analysis
    Binding sitei322 – 3221SubstrateBy similarity

    GO - Molecular functioni

    1. alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. in utero embryonic development Source: Ensembl
    2. protein glycosylation Source: UniProtKB-UniPathway
    3. UDP-N-acetylglucosamine catabolic process Source: Ensembl

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_199031. N-glycan trimming and elongation in the cis-Golgi.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT13. Glycosyltransferase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC:2.4.1.101)
    Alternative name(s):
    N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
    Short name:
    GNT-I
    Short name:
    GlcNAc-T I
    Gene namesi
    Name:Mgat1
    Synonyms:Gnt1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 10

    Organism-specific databases

    RGDi620097. Mgat1.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 447447Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferasePRO_0000191386Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi115 ↔ 145By similarity
    Disulfide bondi239 ↔ 305By similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiQ09325.
    PRIDEiQ09325.

    PTM databases

    PhosphoSiteiQ09325.

    Expressioni

    Tissue specificityi

    Appears to be present in all tissues.

    Gene expression databases

    GenevestigatoriQ09325.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4565797.
    STRINGi10116.ENSRNOP00000044560.

    Structurei

    3D structure databases

    ProteinModelPortaliQ09325.
    SMRiQ09325. Positions 106-446.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66CytoplasmicSequence Analysis
    Topological domaini30 – 447418LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2923Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 13 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG148227.
    GeneTreeiENSGT00530000063632.
    HOGENOMiHOG000247055.
    HOVERGENiHBG052466.
    InParanoidiQ09325.
    KOiK00726.
    OMAiHEETAQV.
    OrthoDBiEOG7R56SN.
    PhylomeDBiQ09325.
    TreeFamiTF320555.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR004139. Glyco_trans_13.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR10468. PTHR10468. 1 hit.
    PfamiPF03071. GNT-I. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q09325-1 [UniParc]FASTAAdd to Basket

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    MLKKQSAGLV LWGAIIFVGW NALLLLFFWT RPAPGRLPSD SALGDDPASL    50
    TREVIHLAED AEAELERQRG LLQQIKEHYS LWRQRWRVPT VAPPAWPRVP 100
    GTPSPAVIPI LVIACDRSTV RRCLDKLLHY RPSAEHFPII VSQDCGHEET 150
    AQVIASYGTA VTHIRQPDLS NIAVQPDHRK FQGYYKIARH YRWALGQIFN 200
    KFKFPAAVVV EDDLEVAPDF FEYFQATYPL LKADPSLWCV SAWNDNGKEQ 250
    MVDSSKPELL YRTDFFPGLG WLLLADLWAE LEPKWPKAFW DDWMRRPEQR 300
    KGRACIRPEI SRTMTFGRKG VSHGQFFDQH LKFIKLNQQF VPFTQLDLSY 350
    LQREAYDRDF LAQVYGAPQL QVEKVRTNDR KELGEVRVQY TSRDSFKAFA 400
    KALGVMDDLK SGVPRAGYRG IVTFQFRGRR VHLAPPETWN GYDPSWN 447
    Length:447
    Mass (Da):51,586
    Last modified:November 1, 1995 - v1
    Checksum:i1AB54107072C53A2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16302 mRNA. Translation: BAA03807.1.
    BC074010 mRNA. Translation: AAH74010.1.
    PIRiJC2076.
    RefSeqiNP_110488.1. NM_030861.1.
    XP_006246244.1. XM_006246182.1.
    XP_006246245.1. XM_006246183.1.
    XP_006246246.1. XM_006246184.1.
    XP_006246247.1. XM_006246185.1.
    XP_006246248.1. XM_006246186.1.
    UniGeneiRn.2712.

    Genome annotation databases

    EnsembliENSRNOT00000052360; ENSRNOP00000044560; ENSRNOG00000031208.
    GeneIDi81519.
    KEGGirno:81519.
    UCSCiRGD:620097. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16302 mRNA. Translation: BAA03807.1 .
    BC074010 mRNA. Translation: AAH74010.1 .
    PIRi JC2076.
    RefSeqi NP_110488.1. NM_030861.1.
    XP_006246244.1. XM_006246182.1.
    XP_006246245.1. XM_006246183.1.
    XP_006246246.1. XM_006246184.1.
    XP_006246247.1. XM_006246185.1.
    XP_006246248.1. XM_006246186.1.
    UniGenei Rn.2712.

    3D structure databases

    ProteinModelPortali Q09325.
    SMRi Q09325. Positions 106-446.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4565797.
    STRINGi 10116.ENSRNOP00000044560.

    Protein family/group databases

    CAZyi GT13. Glycosyltransferase Family 13.

    PTM databases

    PhosphoSitei Q09325.

    Proteomic databases

    PaxDbi Q09325.
    PRIDEi Q09325.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000052360 ; ENSRNOP00000044560 ; ENSRNOG00000031208 .
    GeneIDi 81519.
    KEGGi rno:81519.
    UCSCi RGD:620097. rat.

    Organism-specific databases

    CTDi 4245.
    RGDi 620097. Mgat1.

    Phylogenomic databases

    eggNOGi NOG148227.
    GeneTreei ENSGT00530000063632.
    HOGENOMi HOG000247055.
    HOVERGENi HBG052466.
    InParanoidi Q09325.
    KOi K00726.
    OMAi HEETAQV.
    OrthoDBi EOG7R56SN.
    PhylomeDBi Q09325.
    TreeFami TF320555.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_199031. N-glycan trimming and elongation in the cis-Golgi.

    Miscellaneous databases

    NextBioi 615029.
    PROi Q09325.

    Gene expression databases

    Genevestigatori Q09325.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR004139. Glyco_trans_13.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR10468. PTHR10468. 1 hit.
    Pfami PF03071. GNT-I. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a cDNA encoding N-acetylglucosaminyltransferase I from rat liver and analysis of its expression in rat tissues."
      Fukada T., Iida K., Kioka N., Sakai H., Komano T.
      Biosci. Biotechnol. Biochem. 58:200-201(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.

    Entry informationi

    Entry nameiMGAT1_RAT
    AccessioniPrimary (citable) accession number: Q09325
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3