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Protein

Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

Gene

Mgat1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-(alpha-D-Man-(1->6))-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-(alpha-D-Man-(1->6))-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-N-Asn-[protein].

Cofactori

Mn2+By similarityNote: The cofactor is mostly bound to the substrate.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei117SubstrateBy similarity1
Binding sitei144SubstrateBy similarity1
Binding sitei190SubstrateBy similarity1
Binding sitei212SubstrateBy similarity1
Metal bindingi213ManganeseBy similarity1
Active sitei291Proton acceptorSequence analysis1
Binding sitei322SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-964739 N-glycan trimming and elongation in the cis-Golgi
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT13 Glycosyltransferase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC:2.4.1.101)
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
Short name:
GNT-I
Short name:
GlcNAc-T I
Gene namesi
Name:Mgat1
Synonyms:Gnt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi620097 Mgat1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 29Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST23
Topological domaini30 – 447LumenalSequence analysisAdd BLAST418

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001913861 – 447Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferaseAdd BLAST447

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi115 ↔ 145By similarity
Disulfide bondi239 ↔ 305By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ09325
PRIDEiQ09325

PTM databases

iPTMnetiQ09325
PhosphoSitePlusiQ09325

Expressioni

Tissue specificityi

Appears to be present in all tissues.

Gene expression databases

BgeeiENSRNOG00000031208
GenevisibleiQ09325 RN

Interactioni

Subunit structurei

Interacts with MGAT4D.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000044560

Structurei

3D structure databases

ProteinModelPortaliQ09325
SMRiQ09325
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 13 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1413 Eukaryota
ENOG410XQD8 LUCA
GeneTreeiENSGT00530000063632
HOGENOMiHOG000247055
HOVERGENiHBG052466
InParanoidiQ09325
KOiK00726
OMAiQDPSLWC
PhylomeDBiQ09325
TreeFamiTF320555

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR004139 Glyco_trans_13
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR10468 PTHR10468, 1 hit
PfamiView protein in Pfam
PF03071 GNT-I, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

Q09325-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKKQSAGLV LWGAIIFVGW NALLLLFFWT RPAPGRLPSD SALGDDPASL
60 70 80 90 100
TREVIHLAED AEAELERQRG LLQQIKEHYS LWRQRWRVPT VAPPAWPRVP
110 120 130 140 150
GTPSPAVIPI LVIACDRSTV RRCLDKLLHY RPSAEHFPII VSQDCGHEET
160 170 180 190 200
AQVIASYGTA VTHIRQPDLS NIAVQPDHRK FQGYYKIARH YRWALGQIFN
210 220 230 240 250
KFKFPAAVVV EDDLEVAPDF FEYFQATYPL LKADPSLWCV SAWNDNGKEQ
260 270 280 290 300
MVDSSKPELL YRTDFFPGLG WLLLADLWAE LEPKWPKAFW DDWMRRPEQR
310 320 330 340 350
KGRACIRPEI SRTMTFGRKG VSHGQFFDQH LKFIKLNQQF VPFTQLDLSY
360 370 380 390 400
LQREAYDRDF LAQVYGAPQL QVEKVRTNDR KELGEVRVQY TSRDSFKAFA
410 420 430 440
KALGVMDDLK SGVPRAGYRG IVTFQFRGRR VHLAPPETWN GYDPSWN
Length:447
Mass (Da):51,586
Last modified:November 1, 1995 - v1
Checksum:i1AB54107072C53A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16302 mRNA Translation: BAA03807.1
BC074010 mRNA Translation: AAH74010.1
PIRiJC2076
RefSeqiNP_110488.1, NM_030861.1
XP_006246244.1, XM_006246182.3
XP_006246245.1, XM_006246183.3
XP_006246246.1, XM_006246184.3
XP_006246247.1, XM_006246185.3
XP_008765937.1, XM_008767715.2
UniGeneiRn.2712

Genome annotation databases

EnsembliENSRNOT00000052360; ENSRNOP00000044560; ENSRNOG00000031208
ENSRNOT00000085832; ENSRNOP00000068827; ENSRNOG00000031208
GeneIDi81519
KEGGirno:81519
UCSCiRGD:620097 rat

Similar proteinsi

Entry informationi

Entry nameiMGAT1_RAT
AccessioniPrimary (citable) accession number: Q09325
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health