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Q09325

- MGAT1_RAT

UniProt

Q09325 - MGAT1_RAT

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Protein

Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

Gene

Mgat1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Cofactori

Manganese. The cofactor is mostly bound to the substrate (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei117 – 1171SubstrateBy similarity
Binding sitei144 – 1441SubstrateBy similarity
Binding sitei190 – 1901SubstrateBy similarity
Binding sitei212 – 2121SubstrateBy similarity
Metal bindingi213 – 2131ManganeseBy similarity
Active sitei291 – 2911Proton acceptorSequence Analysis
Binding sitei322 – 3221SubstrateBy similarity

GO - Molecular functioni

  1. alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. in utero embryonic development Source: Ensembl
  2. protein glycosylation Source: UniProtKB-UniPathway
  3. UDP-N-acetylglucosamine catabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_199031. N-glycan trimming and elongation in the cis-Golgi.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT13. Glycosyltransferase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC:2.4.1.101)
Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
Short name:
GNT-I
Short name:
GlcNAc-T I
Gene namesi
Name:Mgat1
Synonyms:Gnt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi620097. Mgat1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. Golgi apparatus Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferasePRO_0000191386Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi115 ↔ 145By similarity
Disulfide bondi239 ↔ 305By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ09325.
PRIDEiQ09325.

PTM databases

PhosphoSiteiQ09325.

Expressioni

Tissue specificityi

Appears to be present in all tissues.

Gene expression databases

GenevestigatoriQ09325.

Interactioni

Protein-protein interaction databases

MINTiMINT-4565797.
STRINGi10116.ENSRNOP00000044560.

Structurei

3D structure databases

ProteinModelPortaliQ09325.
SMRiQ09325. Positions 106-446.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence Analysis
Topological domaini30 – 447418LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2923Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 13 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG148227.
GeneTreeiENSGT00530000063632.
HOGENOMiHOG000247055.
HOVERGENiHBG052466.
InParanoidiQ09325.
KOiK00726.
OMAiHEETAQV.
OrthoDBiEOG7R56SN.
PhylomeDBiQ09325.
TreeFamiTF320555.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR004139. Glyco_trans_13.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10468. PTHR10468. 1 hit.
PfamiPF03071. GNT-I. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q09325-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLKKQSAGLV LWGAIIFVGW NALLLLFFWT RPAPGRLPSD SALGDDPASL
60 70 80 90 100
TREVIHLAED AEAELERQRG LLQQIKEHYS LWRQRWRVPT VAPPAWPRVP
110 120 130 140 150
GTPSPAVIPI LVIACDRSTV RRCLDKLLHY RPSAEHFPII VSQDCGHEET
160 170 180 190 200
AQVIASYGTA VTHIRQPDLS NIAVQPDHRK FQGYYKIARH YRWALGQIFN
210 220 230 240 250
KFKFPAAVVV EDDLEVAPDF FEYFQATYPL LKADPSLWCV SAWNDNGKEQ
260 270 280 290 300
MVDSSKPELL YRTDFFPGLG WLLLADLWAE LEPKWPKAFW DDWMRRPEQR
310 320 330 340 350
KGRACIRPEI SRTMTFGRKG VSHGQFFDQH LKFIKLNQQF VPFTQLDLSY
360 370 380 390 400
LQREAYDRDF LAQVYGAPQL QVEKVRTNDR KELGEVRVQY TSRDSFKAFA
410 420 430 440
KALGVMDDLK SGVPRAGYRG IVTFQFRGRR VHLAPPETWN GYDPSWN
Length:447
Mass (Da):51,586
Last modified:November 1, 1995 - v1
Checksum:i1AB54107072C53A2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16302 mRNA. Translation: BAA03807.1.
BC074010 mRNA. Translation: AAH74010.1.
PIRiJC2076.
RefSeqiNP_110488.1. NM_030861.1.
XP_006246244.1. XM_006246182.2.
XP_006246245.1. XM_006246183.2.
XP_006246246.1. XM_006246184.2.
XP_006246247.1. XM_006246185.2.
UniGeneiRn.2712.

Genome annotation databases

EnsembliENSRNOT00000052360; ENSRNOP00000044560; ENSRNOG00000031208.
GeneIDi81519.
KEGGirno:81519.
UCSCiRGD:620097. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16302 mRNA. Translation: BAA03807.1 .
BC074010 mRNA. Translation: AAH74010.1 .
PIRi JC2076.
RefSeqi NP_110488.1. NM_030861.1.
XP_006246244.1. XM_006246182.2.
XP_006246245.1. XM_006246183.2.
XP_006246246.1. XM_006246184.2.
XP_006246247.1. XM_006246185.2.
UniGenei Rn.2712.

3D structure databases

ProteinModelPortali Q09325.
SMRi Q09325. Positions 106-446.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4565797.
STRINGi 10116.ENSRNOP00000044560.

Protein family/group databases

CAZyi GT13. Glycosyltransferase Family 13.

PTM databases

PhosphoSitei Q09325.

Proteomic databases

PaxDbi Q09325.
PRIDEi Q09325.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000052360 ; ENSRNOP00000044560 ; ENSRNOG00000031208 .
GeneIDi 81519.
KEGGi rno:81519.
UCSCi RGD:620097. rat.

Organism-specific databases

CTDi 4245.
RGDi 620097. Mgat1.

Phylogenomic databases

eggNOGi NOG148227.
GeneTreei ENSGT00530000063632.
HOGENOMi HOG000247055.
HOVERGENi HBG052466.
InParanoidi Q09325.
KOi K00726.
OMAi HEETAQV.
OrthoDBi EOG7R56SN.
PhylomeDBi Q09325.
TreeFami TF320555.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_199031. N-glycan trimming and elongation in the cis-Golgi.

Miscellaneous databases

NextBioi 615029.
PROi Q09325.

Gene expression databases

Genevestigatori Q09325.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR004139. Glyco_trans_13.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR10468. PTHR10468. 1 hit.
Pfami PF03071. GNT-I. 1 hit.
[Graphical view ]
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of a cDNA encoding N-acetylglucosaminyltransferase I from rat liver and analysis of its expression in rat tissues."
    Fukada T., Iida K., Kioka N., Sakai H., Komano T.
    Biosci. Biotechnol. Biochem. 58:200-201(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiMGAT1_RAT
AccessioniPrimary (citable) accession number: Q09325
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3