Q09325 (MGAT1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
EC=2.4.1.101

Alternative name(s):
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I
Short name=GNT-I
Short name=GlcNAc-T I

Gene names

Name:	Mgat1
Synonyms:	Gnt1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 3-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Cofactor	Manganese. The cofactor is mostly bound to the substrate By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Tissue specificity	Appears to be present in all tissues.
Sequence similarities	Belongs to the glycosyltransferase 13 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein N-linked glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase		PRO_0000191386
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 29	23	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	30 – 447	418	Lumenal Potential
Sites
	Active site	291	1	Proton acceptor Potential
	Metal binding	213	1	Manganese By similarity
	Binding site	117	1	Substrate By similarity
	Binding site	144	1	Substrate By similarity
	Binding site	190	1	Substrate By similarity
	Binding site	212	1	Substrate By similarity
	Binding site	322	1	Substrate By similarity
Amino acid modifications
	Disulfide bond	115 ↔ 145		By similarity
	Disulfide bond	239 ↔ 305		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q09325 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 1AB54107072C53A2	FASTA	447	51,586
	10 20 30 40 50 60 MLKKQSAGLV LWGAIIFVGW NALLLLFFWT RPAPGRLPSD SALGDDPASL TREVIHLAED 70 80 90 100 110 120 AEAELERQRG LLQQIKEHYS LWRQRWRVPT VAPPAWPRVP GTPSPAVIPI LVIACDRSTV 130 140 150 160 170 180 RRCLDKLLHY RPSAEHFPII VSQDCGHEET AQVIASYGTA VTHIRQPDLS NIAVQPDHRK 190 200 210 220 230 240 FQGYYKIARH YRWALGQIFN KFKFPAAVVV EDDLEVAPDF FEYFQATYPL LKADPSLWCV 250 260 270 280 290 300 SAWNDNGKEQ MVDSSKPELL YRTDFFPGLG WLLLADLWAE LEPKWPKAFW DDWMRRPEQR 310 320 330 340 350 360 KGRACIRPEI SRTMTFGRKG VSHGQFFDQH LKFIKLNQQF VPFTQLDLSY LQREAYDRDF 370 380 390 400 410 420 LAQVYGAPQL QVEKVRTNDR KELGEVRVQY TSRDSFKAFA KALGVMDDLK SGVPRAGYRG 430 440 IVTFQFRGRR VHLAPPETWN GYDPSWN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a cDNA encoding N-acetylglucosaminyltransferase I from rat liver and analysis of its expression in rat tissues." Fukada T., Iida K., Kioka N., Sakai H., Komano T. Biosci. Biotechnol. Biochem. 58:200-201(1994) [PubMed: 7764514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D16302 mRNA. Translation: BAA03807.1. BC074010 mRNA. Translation: AAH74010.1.
IPI	IPI00203418.
PIR	JC2076.
RefSeq	NP_110488.1. NM_030861.1.
UniGene	Rn.2712.
3D structure databases
ProteinModelPortal	Q09325.
SMR	Q09325. Positions 106-446.
ModBase	Search...
Protein-protein interaction databases
STRING	Q09325.
Protein family/group databases
CAZy	GT13. Glycosyltransferase Family 13.
PTM databases
PhosphoSite	Q09325.
Proteomic databases
PRIDE	Q09325.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000052360; ENSRNOP00000044560; ENSRNOG00000031208.
GeneID	81519.
KEGG	rno:81519.
UCSC	NM_030861. rat.
Organism-specific databases
CTD	4245.
RGD	620097. Mgat1.
Phylogenomic databases
eggNOG	NOG148227.
GeneTree	ENSGT00530000063632.
HOGENOM	HOG000247055.
HOVERGEN	HBG052466.
InParanoid	Q09325.
KO	K00726.
OMA	PPDHRKF.
OrthoDB	EOG4PNXGX.
Gene expression databases
ArrayExpress	Q09325.
Genevestigator	Q09325.
GermOnline	ENSRNOG00000031208. Rattus norvegicus.
Family and domain databases
InterPro	IPR004139. Glyco_trans_13. [Graphical view]
PANTHER	PTHR10468. Glyco_trans_13. 1 hit.
Pfam	PF03071. GNT-I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	615029.

Entry information

Entry name

MGAT1_RAT

Accession

Primary (citable) accession number: Q09325

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 16, 2012
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program