Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase

Gene

Gcnt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Glycosyltransferase that catalyzes the transfer of an N-acetylglucosamine moiety onto mucin-type core 1 O-glycan to form the branched mucin-type core 2 O-glycan. Mucin-type core 2 O-glycans play an important role in leukocyte extravasation as they serve as scaffolds for the display of the selectin ligand sialyl Lewis X by leukocytes.1 Publication

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R = UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3201 Publication1

GO - Molecular functioni

GO - Biological processi

  • cell adhesion molecule production Source: UniProtKB
  • glycoprotein biosynthetic process Source: UniProtKB
  • kidney morphogenesis Source: MGI
  • leukocyte tethering or rolling Source: UniProtKB
  • protein glycosylation Source: UniProtKB-UniPathway
  • response to insulin Source: Ensembl
  • tissue morphogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.102. 3474.
ReactomeiR-MMU-913709. O-linked glycosylation of mucins.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT14. Glycosyltransferase Family 14.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase (EC:2.4.1.102)
Alternative name(s):
Core 2-branching enzyme
Core2-GlcNAc-transferase
Short name:
C2GNT
Gene namesi
Name:Gcnt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:95676. Gcnt1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicSequence analysis9
Transmembranei10 – 32Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST23
Topological domaini33 – 428LumenalSequence analysisAdd BLAST396

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001913961 – 428Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferaseAdd BLAST428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi58N-linked (GlcNAc...)1 Publication1
Disulfide bondi59 ↔ 413
Glycosylationi95N-linked (GlcNAc...)1 Publication1
Disulfide bondi100 ↔ 172
Disulfide bondi151 ↔ 199
Disulfide bondi372 ↔ 381

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ09324.
PaxDbiQ09324.
PRIDEiQ09324.

PTM databases

iPTMnetiQ09324.
PhosphoSitePlusiQ09324.

Expressioni

Tissue specificityi

Expressed in kidney, liver, stomach, spleen, lung and brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000038843.
CleanExiMM_GCNT1.
GenevisibleiQ09324. MM.

Interactioni

Subunit structurei

Interacts with GOLPH3; may control GCNT1 retention in the Golgi.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000069023.

Structurei

Secondary structure

1428
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi59 – 63Combined sources5
Helixi67 – 78Combined sources12
Helixi80 – 83Combined sources4
Helixi90 – 96Combined sources7
Helixi100 – 107Combined sources8
Helixi116 – 119Combined sources4
Beta strandi123 – 129Combined sources7
Helixi133 – 143Combined sources11
Beta strandi148 – 154Combined sources7
Helixi160 – 171Combined sources12
Beta strandi176 – 178Combined sources3
Helixi190 – 206Combined sources17
Beta strandi212 – 217Combined sources6
Beta strandi221 – 224Combined sources4
Helixi226 – 235Combined sources10
Turni236 – 238Combined sources3
Beta strandi244 – 246Combined sources3
Helixi249 – 251Combined sources3
Helixi253 – 256Combined sources4
Beta strandi257 – 262Combined sources6
Beta strandi265 – 272Combined sources8
Beta strandi290 – 292Combined sources3
Helixi293 – 301Combined sources9
Helixi303 – 312Combined sources10
Beta strandi315 – 317Combined sources3
Helixi318 – 320Combined sources3
Helixi322 – 326Combined sources5
Helixi340 – 342Combined sources3
Turni346 – 348Combined sources3
Beta strandi349 – 354Combined sources6
Turni357 – 359Combined sources3
Helixi363 – 365Combined sources3
Beta strandi373 – 377Combined sources5
Beta strandi380 – 382Combined sources3
Helixi385 – 387Combined sources3
Helixi388 – 391Combined sources4
Beta strandi397 – 400Combined sources4
Turni404 – 406Combined sources3
Helixi408 – 423Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GAKX-ray2.00A/B38-428[»]
2GAMX-ray2.70A/B/C/D38-428[»]
3OTKX-ray2.30A/B/C/D38-428[»]
ProteinModelPortaliQ09324.
SMRiQ09324.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ09324.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni5 – 9Mediates interaction with GOLPH3 and is necessary and sufficient for localization to the GolgiBy similarity5
Regioni33 – 121Stem regionAdd BLAST89
Regioni122 – 428CatalyticAdd BLAST307

Sequence similaritiesi

Belongs to the glycosyltransferase 14 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0799. Eukaryota.
ENOG410XQ7M. LUCA.
GeneTreeiENSGT00760000119183.
HOGENOMiHOG000293251.
HOVERGENiHBG051711.
InParanoidiQ09324.
KOiK00727.
OMAiFIKMRKY.
OrthoDBiEOG091G0HLZ.

Family and domain databases

InterProiIPR003406. Glyco_trans_14.
[Graphical view]
PfamiPF02485. Branch. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09324-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRNLFRRRL FSCPTKYYFM LLVLSLITFS VLRIHQKPEF FSVRHLELAG
60 70 80 90 100
DDPYSNVNCT KILQGDPEEI QKVKLEILTV QFKKRPRRTP HDYINMTRDC
110 120 130 140 150
ASFIRTRKYI VEPLTKEEVG FPIAYSIVVH HKIEMLDRLL RAIYMPQNFY
160 170 180 190 200
CIHVDRKAEE SFLAAVQGIA SCFDNVFVAS QLESVVYASW SRVKADLNCM
210 220 230 240 250
KDLYRMNANW KYLINLCGMD FPIKTNLEIV RKLKCSTGEN NLETEKMPPN
260 270 280 290 300
KEERWKKRYT VVDGKLTNTG IVKAPPPLKT PLFSGSAYFV VTREYVGYVL
310 320 330 340 350
ENENIQKLME WAQDTYSPDE FLWATIQRIP EVPGSFPSSN KYDLSDMNAI
360 370 380 390 400
ARFVKWQYFE GHVSNGAPYP PCSGVHVRSV CVFGAGDLSW MLRQHHLFAN
410 420
KFDMDVDPFA IQCLDEHLRH KALENLEH
Length:428
Mass (Da):49,839
Last modified:July 27, 2011 - v2
Checksum:i57E54F07AFFE640B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti88R → W in AAA60948 (Ref. 1) Curated1
Sequence conflicti88R → W in BAA22998 (PubMed:9341170).Curated1
Sequence conflicti88R → W in BAA22999 (PubMed:9341170).Curated1
Sequence conflicti191S → T in AAA60948 (Ref. 1) Curated1
Sequence conflicti238G → A in BAA22998 (PubMed:9341170).Curated1
Sequence conflicti238G → A in BAA22999 (PubMed:9341170).Curated1
Sequence conflicti260T → A in AAA60948 (Ref. 1) Curated1
Sequence conflicti260T → A in BAA22998 (PubMed:9341170).Curated1
Sequence conflicti260T → A in BAA22999 (PubMed:9341170).Curated1
Sequence conflicti362H → D in AAA60948 (Ref. 1) Curated1
Sequence conflicti362H → D in BAA22998 (PubMed:9341170).Curated1
Sequence conflicti362H → D in BAA22999 (PubMed:9341170).Curated1
Sequence conflicti404M → I in BAA22998 (PubMed:9341170).Curated1
Sequence conflicti404M → I in BAA22999 (PubMed:9341170).Curated1
Sequence conflicti420H → R in AAA60948 (Ref. 1) Curated1
Sequence conflicti420H → R in BAA22998 (PubMed:9341170).Curated1
Sequence conflicti420H → R in BAA22999 (PubMed:9341170).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19265 mRNA. Translation: AAA60948.1.
D87332 mRNA. Translation: BAA22998.1.
D87333 mRNA. Translation: BAA22999.1.
AK045216 mRNA. Translation: BAC32265.1.
CCDSiCCDS29687.1.
RefSeqiNP_001129956.3. NM_001136484.3.
NP_034395.4. NM_010265.5.
NP_775618.3. NM_173442.5.
UniGeneiMm.244825.

Genome annotation databases

EnsembliENSMUST00000169897; ENSMUSP00000127835; ENSMUSG00000038843.
ENSMUST00000174236; ENSMUSP00000133935; ENSMUSG00000038843.
GeneIDi14537.
KEGGimmu:14537.
UCSCiuc008gxm.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

core 2 beta 6 GlcNAc T1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19265 mRNA. Translation: AAA60948.1.
D87332 mRNA. Translation: BAA22998.1.
D87333 mRNA. Translation: BAA22999.1.
AK045216 mRNA. Translation: BAC32265.1.
CCDSiCCDS29687.1.
RefSeqiNP_001129956.3. NM_001136484.3.
NP_034395.4. NM_010265.5.
NP_775618.3. NM_173442.5.
UniGeneiMm.244825.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GAKX-ray2.00A/B38-428[»]
2GAMX-ray2.70A/B/C/D38-428[»]
3OTKX-ray2.30A/B/C/D38-428[»]
ProteinModelPortaliQ09324.
SMRiQ09324.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000069023.

Protein family/group databases

CAZyiGT14. Glycosyltransferase Family 14.

PTM databases

iPTMnetiQ09324.
PhosphoSitePlusiQ09324.

Proteomic databases

MaxQBiQ09324.
PaxDbiQ09324.
PRIDEiQ09324.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000169897; ENSMUSP00000127835; ENSMUSG00000038843.
ENSMUST00000174236; ENSMUSP00000133935; ENSMUSG00000038843.
GeneIDi14537.
KEGGimmu:14537.
UCSCiuc008gxm.2. mouse.

Organism-specific databases

CTDi2650.
MGIiMGI:95676. Gcnt1.

Phylogenomic databases

eggNOGiKOG0799. Eukaryota.
ENOG410XQ7M. LUCA.
GeneTreeiENSGT00760000119183.
HOGENOMiHOG000293251.
HOVERGENiHBG051711.
InParanoidiQ09324.
KOiK00727.
OMAiFIKMRKY.
OrthoDBiEOG091G0HLZ.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.1.102. 3474.
ReactomeiR-MMU-913709. O-linked glycosylation of mucins.

Miscellaneous databases

ChiTaRSiGcnt1. mouse.
EvolutionaryTraceiQ09324.
PROiQ09324.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038843.
CleanExiMM_GCNT1.
GenevisibleiQ09324. MM.

Family and domain databases

InterProiIPR003406. Glyco_trans_14.
[Graphical view]
PfamiPF02485. Branch. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGCNT1_MOUSE
AccessioniPrimary (citable) accession number: Q09324
Secondary accession number(s): O35981, Q8BRB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.