ID   5NT3_CAEEL              Reviewed;         376 AA.
AC   Q09315; Q95QJ1;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2002, sequence version 2.
DT   16-JUN-2009, entry version 49.
DE   RecName: Full=Putative cytosolic 5'-nucleotidase 3;
DE            EC=3.1.3.5;
DE   AltName: Full=Putative pyrimidine 5'-nucleotidase;
GN   ORFNames=F25B5.3;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Nematoda; Chromadorea; Rhabditida; Rhabditoidea;
OC   Rhabditidae; Peloderinae; Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE
RP   SPLICING.
RC   STRAIN=Bristol N2;
RX   MEDLINE=99069613; PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for
RT   investigating biology.";
RL   Science 282:2012-2018(1998).
CC   -!- CATALYTIC ACTIVITY: A 5'-ribonucleotide + H(2)O = a ribonucleoside
CC       + phosphate.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC         Comment=Experimental confirmation may be lacking for some
CC         isoforms;
CC       Name=b;
CC         IsoId=Q09315-1; Sequence=Displayed;
CC       Name=a;
CC         IsoId=Q09315-2; Sequence=VSP_002447;
CC       Name=c;
CC         IsoId=Q09315-3; Sequence=VSP_002446;
CC   -!- SIMILARITY: Belongs to the pyrimidine 5'-nucleotidase family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; U23172; AAK67227.1; -; Genomic_DNA.
DR   EMBL; U23172; AAK67228.1; -; Genomic_DNA.
DR   EMBL; U23172; AAL27245.1; -; Genomic_DNA.
DR   RefSeq; NP_498293.1; -.
DR   UniGene; Cel.17714; -.
DR   IntAct; Q09315; 1.
DR   Ensembl; F25B5.3; Caenorhabditis elegans.
DR   GeneID; 175843; -.
DR   KEGG; cel:F25B5.3; -.
DR   WormBase; WBGene00017775; F25B5.3.
DR   WormPep; F25B5.3a; CE26890.
DR   WormPep; F25B5.3b; CE28001.
DR   WormPep; F25B5.3c; CE29776.
DR   OMA; Q09315; EKIPHME.
DR   BRENDA; 3.1.3.5; 672.
DR   NextBio; 889908; -.
DR   ArrayExpress; Q09315; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008253; F:5'-nucleotidase activity; IEA:EC.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0009117; P:nucleotide metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR006434; HAD-SF_hydro_IE.
DR   InterPro; IPR018012; HAD-SF_hydro_IE_pln.
DR   PANTHER; PTHR13045; HAD-SF_hydro_IE; 1.
DR   Pfam; PF05822; UMPH-1; 1.
DR   TIGRFAMs; TIGR01544; HAD-SF-IE; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Complete proteome; Cytoplasm; Hydrolase;
KW   Magnesium; Metal-binding; Nucleotide metabolism; Nucleotide-binding.
FT   CHAIN         1    376       Putative cytosolic 5'-nucleotidase 3.
FT                                /FTId=PRO_0000065315.
FT   REGION      236    237       Substrate binding (By similarity).
FT   ACT_SITE    119    119       Nucleophile (By similarity).
FT   ACT_SITE    121    121       Proton donor (By similarity).
FT   METAL       119    119       Magnesium (By similarity).
FT   METAL       121    121       Magnesium; via carbonyl oxygen (By
FT                                similarity).
FT   METAL       312    312       Magnesium (By similarity).
FT   BINDING     286    286       Substrate (By similarity).
FT   VAR_SEQ       1     49       Missing (in isoform c).
FT                                /FTId=VSP_002446.
FT   VAR_SEQ       1     30       MSNKVARRLGKCLFVSGRRFESRQSILQLR -> MGHCFSV
FT                                FSSKETINC (in isoform a).
FT                                /FTId=VSP_002447.
SQ   SEQUENCE   376 AA;  42146 MW;  C5077AB7A8C8D979 CRC64;
     MSNKVARRLG KCLFVSGRRF ESRQSILQLR TETLTDTPLS ATLDQSQFSM FKAAEIVNAA
     AACAEAECIE QLKKTDVVPL LMNYLLGEEQ ILVADPTAVA AKLRKMVVGG AGKTVVISDF
     DYTLSRFANE QGERLSTTHG VFDDNVMRLK PELGQKFVDL KNKYYPIEFS PNLTMEEKIP
     HMEKWWGTSH SLIVNEKFSK NTIEDFVRQS RIVFKDGAED FIEALDAHNI PLVIFSAGIG
     NIIEYFLQQK LGAIPRNTHF ISNMILFDED DNACAFSEPL IHTFCKNSSV IQKETSFFHD
     IAGRVNVILL GDSMGDIHMD VGVERDGPTL KVGYYNGSLD DTAALQHYEE VYDIVLIHDP
     TLNVAQKIVD IINSSH
//