Reviewed, UniProtKB/Swiss-Prot Q09315 (5NT3_CAEEL)

Names and origin

Protein names

Recommended name:
    Putative cytosolic 5'-nucleotidase 3
    EC=3.1.3.5
Alternative name(s):
    Putative pyrimidine 5'-nucleotidase

Gene names

ORF Names:

F25B5.3

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the pyrimidine 5'-nucleotidase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	5'-nucleotidase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.

Isoform b (identifier: Q09315-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform a (identifier: Q09315-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-30: MSNKVARRLGKCLFVSGRRFESRQSILQLR → MGHCFSVFSSKETINC

Isoform c (identifier: Q09315-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Putative cytosolic 5'-nucleotidase 3		PRO_0000065315
Regions
	Region	236 – 237	2	Substrate binding By similarity
Sites
	Active site	119	1	Nucleophile By similarity
	Active site	121	1	Proton donor By similarity
	Metal binding	119	1	Magnesium By similarity
	Metal binding	121	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	312	1	Magnesium By similarity
	Binding site	286	1	Substrate By similarity
Natural variations
	Alternative sequence	1 – 49	49	Missing in isoform c.		VSP_002446
	Alternative sequence	1 – 30	30	MSNKV…ILQLR → MGHCFSVFSSKETINC in isoform a.		VSP_002447

Sequences

	Sequence		Length	Mass (Da)
	Isoform b [UniParc]. Last modified August 2, 2002. Version 2. Checksum: C5077AB7A8C8D979	FASTA	376	42,146
	10 20 30 40 50 60 MSNKVARRLG KCLFVSGRRF ESRQSILQLR TETLTDTPLS ATLDQSQFSM FKAAEIVNAA 70 80 90 100 110 120 AACAEAECIE QLKKTDVVPL LMNYLLGEEQ ILVADPTAVA AKLRKMVVGG AGKTVVISDF 130 140 150 160 170 180 DYTLSRFANE QGERLSTTHG VFDDNVMRLK PELGQKFVDL KNKYYPIEFS PNLTMEEKIP 190 200 210 220 230 240 HMEKWWGTSH SLIVNEKFSK NTIEDFVRQS RIVFKDGAED FIEALDAHNI PLVIFSAGIG 250 260 270 280 290 300 NIIEYFLQQK LGAIPRNTHF ISNMILFDED DNACAFSEPL IHTFCKNSSV IQKETSFFHD 310 320 330 340 350 360 IAGRVNVILL GDSMGDIHMD VGVERDGPTL KVGYYNGSLD DTAALQHYEE VYDIVLIHDP 370 TLNVAQKIVD IINSSH
	Isoform a. Checksum: 5A3D9B64BB1D7AB1	FASTA	362	40,399
	10 20 30 40 50 60 MGHCFSVFSS KETINCTETL TDTPLSATLD QSQFSMFKAA EIVNAAAACA EAECIEQLKK 70 80 90 100 110 120 TDVVPLLMNY LLGEEQILVA DPTAVAAKLR KMVVGGAGKT VVISDFDYTL SRFANEQGER 130 140 150 160 170 180 LSTTHGVFDD NVMRLKPELG QKFVDLKNKY YPIEFSPNLT MEEKIPHMEK WWGTSHSLIV 190 200 210 220 230 240 NEKFSKNTIE DFVRQSRIVF KDGAEDFIEA LDAHNIPLVI FSAGIGNIIE YFLQQKLGAI 250 260 270 280 290 300 PRNTHFISNM ILFDEDDNAC AFSEPLIHTF CKNSSVIQKE TSFFHDIAGR VNVILLGDSM 310 320 330 340 350 360 GDIHMDVGVE RDGPTLKVGY YNGSLDDTAA LQHYEEVYDI VLIHDPTLNV AQKIVDIINS SH
	Isoform c. Checksum: 106750F79A6EDA98	FASTA	327	36,590
	10 20 30 40 50 60 MFKAAEIVNA AAACAEAECI EQLKKTDVVP LLMNYLLGEE QILVADPTAV AAKLRKMVVG 70 80 90 100 110 120 GAGKTVVISD FDYTLSRFAN EQGERLSTTH GVFDDNVMRL KPELGQKFVD LKNKYYPIEF 130 140 150 160 170 180 SPNLTMEEKI PHMEKWWGTS HSLIVNEKFS KNTIEDFVRQ SRIVFKDGAE DFIEALDAHN 190 200 210 220 230 240 IPLVIFSAGI GNIIEYFLQQ KLGAIPRNTH FISNMILFDE DDNACAFSEP LIHTFCKNSS 250 260 270 280 290 300 VIQKETSFFH DIAGRVNVIL LGDSMGDIHM DVGVERDGPT LKVGYYNGSL DDTAALQHYE 310 320 EVYDIVLIHD PTLNVAQKIV DIINSSH

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U23172 Genomic DNA. Translation: AAK67227.1. U23172 Genomic DNA. Translation: AAK67228.1. U23172 Genomic DNA. Translation: AAL27245.1.
RefSeq	NP_498293.1.
UniGene	Cel.17714
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q09315. 1 interaction.
Genome annotation databases
Ensembl	F25B5.3. Caenorhabditis elegans. [Contig view]
GeneID	175843.
KEGG	cel:F25B5.3.
Organism-specific databases
WormBase	WBGene00017775. F25B5.3.
WormPep	F25B5.3a. CE26890. [WorfDB] F25B5.3b. CE28001. [WorfDB] F25B5.3c. CE29776. [WorfDB]
Phylogenomic databases
OMA	Q09315. EKIPHME.
Enzyme and pathway databases
BRENDA	3.1.3.5. 672.
Gene expression databases
ArrayExpress	Q09315.
Family and domain databases
InterPro	IPR006434. HAD-SF_hydro_IE. IPR018012. HAD-SF_hydro_IE_pln. [Graphical view]
PANTHER	PTHR13045. HAD-SF_hydro_IE. 1 hit.
Pfam	PF05822. UMPH-1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01544. HAD-SF-IE. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	889908.

Entry information

Entry name

5NT3_CAEEL

Accession

Primary (citable) accession number: Q09315
Secondary accession number(s): Q95QJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	August 2, 2002
Last modified:	June 16, 2009
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project