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Q09260

- LAG3_CAEEL

UniProt

Q09260 - LAG3_CAEEL

Protein

Protein lag-3

Gene

sel-8

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 2 (06 Jun 2002)
      Previous versions | rss
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    Functioni

    Glp-1 and lin-12 promote signalling by recruiting lag-3 to target promoters, where it functions as a transcriptional activator.

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Enzyme and pathway databases

    SignaLinkiQ09260.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein lag-3
    Alternative name(s):
    Abnormal cell lineage protein 3
    Abnormal germline proliferation protein 3
    Gene namesi
    Name:sel-8
    Synonyms:lag-3
    ORF Names:C32A3.1
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome III

    Organism-specific databases

    WormBaseiC32A3.1a; CE27810; WBGene00004765; sel-8.
    C32A3.1b; CE01505; WBGene00004765; sel-8.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: WormBase

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 490490Protein lag-3PRO_0000084353Add
    BLAST

    Proteomic databases

    PaxDbiQ09260.
    PRIDEiQ09260.

    Interactioni

    Subunit structurei

    Associates with lag-1.

    Protein-protein interaction databases

    BioGridi40704. 11 interactions.
    IntActiQ09260. 3 interactions.
    MINTiMINT-1076351.
    STRINGi6239.C32A3.1a.

    Structurei

    Secondary structure

    1
    490
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi60 – 8324
    Helixi85 – 9814
    Helixi100 – 11213

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FO1X-ray3.12D49-132[»]
    ProteinModelPortaliQ09260.
    SMRiQ09260. Positions 52-114.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ09260.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi162 – 467306Gln-richAdd
    BLAST
    Compositional biasi185 – 1884Poly-Gln
    Compositional biasi341 – 3466Poly-Gln
    Compositional biasi353 – 3575Poly-Gln
    Compositional biasi372 – 3809Poly-Gln
    Compositional biasi390 – 40011Poly-GlnAdd
    BLAST
    Compositional biasi433 – 44210Poly-Gln

    Phylogenomic databases

    eggNOGiNOG250594.
    HOGENOMiHOG000019032.
    InParanoidiQ09260.
    OMAiGEWFDKL.
    OrthoDBiEOG7GTT8G.

    Family and domain databases

    InterProiIPR021587. Transcription_activator_LAG-3.
    [Graphical view]
    PfamiPF11498. Activator_LAG-3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform a (identifier: Q09260-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDDLSEFFVI EEMFISEPSV AGMKPSTSKT THSPPPEEPT APFVNDNLPN    50
    PEDEPTIGDL NAFHSGEELH RQRSELARAN YEKARPEMIA NQRAVTAHLF 100
    NRYTEDEERK RVEQQKNKEA MNASTSAPTS SRNGGQSVEN RKRRNDVVVA 150
    PPTSEEEWKR AQQQHWMGQQ QPQMQFQMQQ QYHSQQQQYI MMQQQHHHMT 200
    GMQQIHHQMP STSSADSIRS VPTPASSMHQ PSPAEMRNGC GMSRNATMDM 250
    TCSPMSGGQP IVDENNLAVP EGEWFDKLAL AVAEQYNVDT ILGPDTYDTF 300
    LAELDFSSSE SPTKQSPMEM NGDRMPSTAP PPAQNPQHIA QLQQQQNKMR 350
    LMQQQQQEMQ RIEQQRRQQI MQQQQQQQQQ EHQRQQMLLQ QQQQQQQMQQ 400
    HHQMNGGGQF ATQAHQQAAY MQQMQRMEQI RHQQQQAQQH QQAQQQHQQQ 450
    AQHHQMGYGI PNGYPQQMHM HPPAYGAHHM PQPTAFANIN 490
    Length:490
    Mass (Da):56,482
    Last modified:June 6, 2002 - v2
    Checksum:i396A6C194C382A07
    GO
    Isoform b (identifier: Q09260-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-22: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:468
    Mass (Da):54,008
    Checksum:iB50E623F5AB4D7DB
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2222Missing in isoform b. 1 PublicationVSP_003908Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF241847 mRNA. Translation: AAF71523.1.
    AF241846 mRNA. Translation: AAF71522.1.
    Z48241 Genomic DNA. Translation: CAA88284.1.
    Z48241 Genomic DNA. Translation: CAC42265.1.
    PIRiT19628.
    RefSeqiNP_001021194.1. NM_001026023.2. [Q09260-1]
    NP_001021195.1. NM_001026024.2. [Q09260-2]
    UniGeneiCel.19503.

    Genome annotation databases

    EnsemblMetazoaiC32A3.1a; C32A3.1a; WBGene00004765. [Q09260-1]
    GeneIDi175464.
    KEGGicel:CELE_C32A3.1.
    UCSCiC32A3.1b.2. c. elegans. [Q09260-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF241847 mRNA. Translation: AAF71523.1 .
    AF241846 mRNA. Translation: AAF71522.1 .
    Z48241 Genomic DNA. Translation: CAA88284.1 .
    Z48241 Genomic DNA. Translation: CAC42265.1 .
    PIRi T19628.
    RefSeqi NP_001021194.1. NM_001026023.2. [Q09260-1 ]
    NP_001021195.1. NM_001026024.2. [Q09260-2 ]
    UniGenei Cel.19503.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2FO1 X-ray 3.12 D 49-132 [» ]
    ProteinModelPortali Q09260.
    SMRi Q09260. Positions 52-114.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 40704. 11 interactions.
    IntActi Q09260. 3 interactions.
    MINTi MINT-1076351.
    STRINGi 6239.C32A3.1a.

    Proteomic databases

    PaxDbi Q09260.
    PRIDEi Q09260.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai C32A3.1a ; C32A3.1a ; WBGene00004765 . [Q09260-1 ]
    GeneIDi 175464.
    KEGGi cel:CELE_C32A3.1.
    UCSCi C32A3.1b.2. c. elegans. [Q09260-1 ]

    Organism-specific databases

    CTDi 175464.
    WormBasei C32A3.1a ; CE27810 ; WBGene00004765 ; sel-8.
    C32A3.1b ; CE01505 ; WBGene00004765 ; sel-8.

    Phylogenomic databases

    eggNOGi NOG250594.
    HOGENOMi HOG000019032.
    InParanoidi Q09260.
    OMAi GEWFDKL.
    OrthoDBi EOG7GTT8G.

    Enzyme and pathway databases

    SignaLinki Q09260.

    Miscellaneous databases

    EvolutionaryTracei Q09260.
    NextBioi 888270.

    Family and domain databases

    InterProi IPR021587. Transcription_activator_LAG-3.
    [Graphical view ]
    Pfami PF11498. Activator_LAG-3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "LAG-3 is a putative transcriptional activator in the C. elegans Notch pathway."
      Petcherski A.G., Kimble J.
      Nature 405:364-368(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B).
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.

    Entry informationi

    Entry nameiLAG3_CAEEL
    AccessioniPrimary (citable) accession number: Q09260
    Secondary accession number(s): Q9NGS2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: June 6, 2002
    Last modified: October 1, 2014
    This is version 100 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3