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Q09260 (LAG3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein lag-3
Alternative name(s):
Abnormal cell lineage protein 3
Abnormal germline proliferation protein 3
Gene names
Name:sel-8
Synonyms:lag-3
ORF Names:C32A3.1
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length490 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Glp-1 and lin-12 promote signalling by recruiting lag-3 to target promoters, where it functions as a transcriptional activator.

Subunit structure

Associates with lag-1.

Subcellular location

Nucleus.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   Molecular functionActivator
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processregulation of transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from direct assay Ref.1PubMed 10884418. Source: WormBase

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: Q09260-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform b (identifier: Q09260-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 490490Protein lag-3
PRO_0000084353

Regions

Compositional bias162 – 467306Gln-rich
Compositional bias185 – 1884Poly-Gln
Compositional bias341 – 3466Poly-Gln
Compositional bias353 – 3575Poly-Gln
Compositional bias372 – 3809Poly-Gln
Compositional bias390 – 40011Poly-Gln
Compositional bias433 – 44210Poly-Gln

Natural variations

Alternative sequence1 – 2222Missing in isoform b.
VSP_003908

Secondary structure

....... 490
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified June 6, 2002. Version 2.
Checksum: 396A6C194C382A07

FASTA49056,482
        10         20         30         40         50         60 
MDDLSEFFVI EEMFISEPSV AGMKPSTSKT THSPPPEEPT APFVNDNLPN PEDEPTIGDL 

        70         80         90        100        110        120 
NAFHSGEELH RQRSELARAN YEKARPEMIA NQRAVTAHLF NRYTEDEERK RVEQQKNKEA 

       130        140        150        160        170        180 
MNASTSAPTS SRNGGQSVEN RKRRNDVVVA PPTSEEEWKR AQQQHWMGQQ QPQMQFQMQQ 

       190        200        210        220        230        240 
QYHSQQQQYI MMQQQHHHMT GMQQIHHQMP STSSADSIRS VPTPASSMHQ PSPAEMRNGC 

       250        260        270        280        290        300 
GMSRNATMDM TCSPMSGGQP IVDENNLAVP EGEWFDKLAL AVAEQYNVDT ILGPDTYDTF 

       310        320        330        340        350        360 
LAELDFSSSE SPTKQSPMEM NGDRMPSTAP PPAQNPQHIA QLQQQQNKMR LMQQQQQEMQ 

       370        380        390        400        410        420 
RIEQQRRQQI MQQQQQQQQQ EHQRQQMLLQ QQQQQQQMQQ HHQMNGGGQF ATQAHQQAAY 

       430        440        450        460        470        480 
MQQMQRMEQI RHQQQQAQQH QQAQQQHQQQ AQHHQMGYGI PNGYPQQMHM HPPAYGAHHM 

       490 
PQPTAFANIN 

« Hide

Isoform b [UniParc].

Checksum: B50E623F5AB4D7DB
Show »

FASTA46854,008

References

« Hide 'large scale' references
[1]"LAG-3 is a putative transcriptional activator in the C. elegans Notch pathway."
Petcherski A.G., Kimble J.
Nature 405:364-368(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B).
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF241847 mRNA. Translation: AAF71523.1.
AF241846 mRNA. Translation: AAF71522.1.
Z48241 Genomic DNA. Translation: CAA88284.1.
Z48241 Genomic DNA. Translation: CAC42265.1.
PIRT19628.
RefSeqNP_001021194.1. NM_001026023.2. [Q09260-1]
NP_001021195.1. NM_001026024.2. [Q09260-2]
UniGeneCel.19503.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2FO1X-ray3.12D49-132[»]
ProteinModelPortalQ09260.
SMRQ09260. Positions 52-114.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid40704. 11 interactions.
IntActQ09260. 3 interactions.
MINTMINT-1076351.
STRING6239.C32A3.1a.

Proteomic databases

PaxDbQ09260.
PRIDEQ09260.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaC32A3.1a; C32A3.1a; WBGene00004765. [Q09260-1]
GeneID175464.
KEGGcel:CELE_C32A3.1.
UCSCC32A3.1b.2. c. elegans. [Q09260-1]

Organism-specific databases

CTD175464.
WormBaseC32A3.1a; CE27810; WBGene00004765; sel-8.
C32A3.1b; CE01505; WBGene00004765; sel-8.

Phylogenomic databases

eggNOGNOG250594.
HOGENOMHOG000019032.
InParanoidQ09260.
OMAGEWFDKL.
OrthoDBEOG7GTT8G.

Enzyme and pathway databases

SignaLinkQ09260.

Family and domain databases

InterProIPR021587. Transcription_activator_LAG-3.
[Graphical view]
PfamPF11498. Activator_LAG-3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ09260.
NextBio888270.

Entry information

Entry nameLAG3_CAEEL
AccessionPrimary (citable) accession number: Q09260
Secondary accession number(s): Q9NGS2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 6, 2002
Last modified: July 9, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase