Reviewed, UniProtKB/Swiss-Prot Q09200 (B4GN1_MOUSE)

Names and origin

Protein names

Recommended name:
    Beta-1,4 N-acetylgalactosaminyltransferase 1
    EC=2.4.1.92
Alternative name(s):
    GalNAc-T
    (N-acetylneuraminyl)-galactosylglucosylceramide
    GM2/GD2 synthase

Gene names

Name:	B4galnt1
Synonyms:	Galgt, Galgt1, Ggm2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the biosynthesis of gangliosides GM2, GD2 and GA2.
Catalytic activity	UDP-N-acetyl-D-galactosamine + 1-O-(O-(N-acetyl-alpha-neuraminosyl)-(2->3)-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl)-ceramide = UDP + 1-O-(O-2-(acetylamino)-2-deoxy-beta-D-galactopyranosyl-(1->4)-O-(N-acetyl-alpha-neuraminosyl-(2->3))-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl)-ceramide.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Homodimer; disulfide-linked By similarity.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Tissue specificity	Most abundant in brain, liver, lung, spleen and testis.
Developmental stage	Highest at day 7 of embryonic development after which it declines to its lowest levels at day 11 before increasing again.
Sequence similarities	Belongs to the glycosyltransferase 2 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	ganglioside biosynthetic process Inferred from genetic interaction. Source: MGI lipid glycosylation Inferred from electronic annotation. Source: InterPro lipid storage Inferred from genetic interaction. Source: MGI spermatogenesis Inferred from mutant phenotype. Source: MGI
Cellular component	integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular function	(N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 533	533	Beta-1,4 N-acetylgalactosaminyltransferase 1		PRO_0000059101
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 25	18	Signal-anchor for type II membrane protein Potential
	Topological domain	26 – 533	508	Lumenal Potential
Amino acid modifications
	Glycosylation	79	1	N-linked (GlcNAc...) Potential
	Glycosylation	179	1	N-linked (GlcNAc...) Potential
	Glycosylation	274	1	N-linked (GlcNAc...) Potential
	Disulfide bond	80		Interchain (with C-412) By similarity
	Disulfide bond	82		Interchain (with C-529) By similarity
	Disulfide bond	412		Interchain (with C-80) By similarity
	Disulfide bond	429 ↔ 476		By similarity
	Disulfide bond	529		Interchain (with C-82) By similarity
Experimental info
	Sequence conflict	73	1	G → E in AAA85496. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q09200-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 6A15D81D2A68A28F	FASTA	533	59,212
	10 20 30 40 50 60 MRLDRRALYA LVLLLACASL GLLYSSTRNA PSLPNPLALW SPPQGPPRLD LLDLAPEPRY 70 80 90 100 110 120 AHIPVRIKEQ VVGLLAQNNC SCESKGGSLP LPFLRQVRAV DLTKAFDAEE LRAVSVAREQ 130 140 150 160 170 180 EYQAFLARSR SLADQLLIAP ANSPLQYPLQ GVEVQPLRSI LVPGLSLQEA SVQEIYQVNL 190 200 210 220 230 240 SASLGTWDVA GEVTGVTLTG EGQPDLTLAS PVLDKLNRQL QLVTYSSRSY QANTADTVRF 250 260 270 280 290 300 STKGHEVAFT ILVRHPPNPR LYPPSSLPQG AEYNISALVT IATKTFLRYD RLRTLIASIR 310 320 330 340 350 360 RFYPTVTIVI ADDSDKPERI SDPHVEHYFM PFGKGWFAGR NLAVSQVTTK YVLWVDDDFV 370 380 390 400 410 420 FTARTRLEKL VDVLEKTPLD LVGGAVREIS GYATTYRQLL SVEPGAPGLG NCFRQKQGFH 430 440 450 460 470 480 HELVGFPSCV VTDGVVNFFL ARTDKVRQVG FDPRLNRVAH LEFFLDGLGF LRVGSCSDVV 490 500 510 520 530 VDHASKVKLP WTAKDPGAET YARYRYPGSL DQSQVAKHRL LFFKHRLQCM TAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"T cell receptor-mediated stimulation of mouse thymocytes induces up-regulation of the GM2/GD2 synthase gene." Takamiya K., Yamamoto A., Yamashiro S., Furukawa K., Haraguchi M., Okada M., Ikeda T., Shiku H., Furukawa K. FEBS Lett. 358:79-83(1995) [PubMed: 7821435] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Beta-1,4-N-acetylgalactosaminyltransferase involved in ganglioside synthesis: cDNA sequence, expression, and chromosome mapping of the mouse gene." Sango K., Johnson O.N., Kozak C.A., Proia R.L. Genomics 27:362-365(1995) [PubMed: 7558008] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Neonatal brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: 129. Tissue: Mammary gland.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

Beta-1,4 N-acetylgalactosaminyltransferase 1

Cross-references

Sequence databases
EMBLGenBankDDBJ	L25885 mRNA. Translation: AAA65027.1. U18975 mRNA. Translation: AAA85496.1. BC036996 mRNA. Translation: AAH36996.1. BC057199 mRNA. Translation: AAH57199.1.
IPI	IPI00122592.
PIR	S78529.
RefSeq	NP_032106.1. NP_082015.1.
UniGene	Mm.386762
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT12. Glycosyltransferase Family 12.
Proteomic databases
PRIDE	Q09200.
Genome annotation databases
Ensembl	ENSMUSG00000006731. Mus musculus. [Contig view]
GeneID	14421.
KEGG	mmu:14421.
Organism-specific databases
MGI	MGI:1342057. B4galnt1.
Phylogenomic databases
HOGENOM	Q09200.
HOVERGEN	Q09200.
OMA	Q09200. APGLGNC.
Enzyme and pathway databases
BRENDA	2.4.1.92. 244.
Gene expression databases
Bgee	Q09200.
CleanEx	MM_B4GALNT1.
GermOnline	ENSMUSG00000006731. Mus musculus.
Family and domain databases
InterPro	IPR001173. Glyco_trans_2. IPR011143. GM2_synthase. [Graphical view]
Pfam	PF00535. Glycos_transf_2. 1 hit. [Graphical view]
PIRSF	PIRSF000474. GM2_GD2_synthase. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	286013.
SOURCE	Search...

Entry information

Entry name

B4GN1_MOUSE

Accession

Primary (citable) accession number: Q09200

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 5, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)