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Q09200

- B4GN1_MOUSE

UniProt

Q09200 - B4GN1_MOUSE

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Protein

Beta-1,4 N-acetylgalactosaminyltransferase 1

Gene
B4galnt1, Galgt, Galgt1, Ggm2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in the biosynthesis of gangliosides GM2, GD2 and GA2.

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + O-(N-acetyl-alpha-neuraminyl)-(2->3)-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->1)-ceramide = UDP + O-2-(acetylamino)-2-deoxy-beta-D-galactopyranosyl-(1->4)-O-(N-acetyl-alpha-neuraminyl-(2->3))-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->1)-ceramide.

Pathwayi

GO - Molecular functioni

  1. (N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. ganglioside biosynthetic process Source: MGI
  2. glycosphingolipid metabolic process Source: MGI
  3. lipid glycosylation Source: InterPro
  4. lipid storage Source: MGI
  5. protein glycosylation Source: UniProtKB-UniPathway
  6. spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT12. Glycosyltransferase Family 12.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4 N-acetylgalactosaminyltransferase 1 (EC:2.4.1.92)
Alternative name(s):
(N-acetylneuraminyl)-galactosylglucosylceramide
GM2/GD2 synthase
GalNAc-T
Gene namesi
Name:B4galnt1
Synonyms:Galgt, Galgt1, Ggm2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:1342057. B4galnt1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77Cytoplasmic Reviewed prediction
Transmembranei8 – 2518Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST
Topological domaini26 – 533508Lumenal Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of Golgi membrane Source: InterPro
  2. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 533533Beta-1,4 N-acetylgalactosaminyltransferase 1PRO_0000059101Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi79 – 791N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi80 – 80Interchain (with C-412) By similarity
Disulfide bondi82 – 82Interchain (with C-529) By similarity
Glycosylationi179 – 1791N-linked (GlcNAc...) Reviewed prediction
Glycosylationi274 – 2741N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi412 – 412Interchain (with C-80) By similarity
Disulfide bondi429 ↔ 476 By similarity
Disulfide bondi529 – 529Interchain (with C-82) By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ09200.
PaxDbiQ09200.
PRIDEiQ09200.

PTM databases

PhosphoSiteiQ09200.

Expressioni

Tissue specificityi

Most abundant in brain, liver, lung, spleen and testis.

Developmental stagei

Highest at day 7 of embryonic development after which it declines to its lowest levels at day 11 before increasing again.

Gene expression databases

ArrayExpressiQ09200.
BgeeiQ09200.
CleanExiMM_B4GALNT1.
GenevestigatoriQ09200.

Interactioni

Subunit structurei

Homodimer; disulfide-linked By similarity.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000006914.

Structurei

3D structure databases

ProteinModelPortaliQ09200.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG40821.
HOGENOMiHOG000220853.
HOVERGENiHBG004812.
InParanoidiQ09200.
KOiK00725.
OMAiIEHYLMP.
OrthoDBiEOG7R2BJS.
PhylomeDBiQ09200.
TreeFamiTF332297.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR011143. GM2_synthase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000474. GM2_GD2_synthase. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q09200-1 [UniParc]FASTAAdd to Basket

« Hide

MRLDRRALYA LVLLLACASL GLLYSSTRNA PSLPNPLALW SPPQGPPRLD    50
LLDLAPEPRY AHIPVRIKEQ VVGLLAQNNC SCESKGGSLP LPFLRQVRAV 100
DLTKAFDAEE LRAVSVAREQ EYQAFLARSR SLADQLLIAP ANSPLQYPLQ 150
GVEVQPLRSI LVPGLSLQEA SVQEIYQVNL SASLGTWDVA GEVTGVTLTG 200
EGQPDLTLAS PVLDKLNRQL QLVTYSSRSY QANTADTVRF STKGHEVAFT 250
ILVRHPPNPR LYPPSSLPQG AEYNISALVT IATKTFLRYD RLRTLIASIR 300
RFYPTVTIVI ADDSDKPERI SDPHVEHYFM PFGKGWFAGR NLAVSQVTTK 350
YVLWVDDDFV FTARTRLEKL VDVLEKTPLD LVGGAVREIS GYATTYRQLL 400
SVEPGAPGLG NCFRQKQGFH HELVGFPSCV VTDGVVNFFL ARTDKVRQVG 450
FDPRLNRVAH LEFFLDGLGF LRVGSCSDVV VDHASKVKLP WTAKDPGAET 500
YARYRYPGSL DQSQVAKHRL LFFKHRLQCM TAE 533
Length:533
Mass (Da):59,212
Last modified:November 1, 1995 - v1
Checksum:i6A15D81D2A68A28F
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731G → E in AAA85496. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L25885 mRNA. Translation: AAA65027.1.
U18975 mRNA. Translation: AAA85496.1.
BC036996 mRNA. Translation: AAH36996.1.
BC057199 mRNA. Translation: AAH57199.1.
CCDSiCCDS24230.1.
PIRiS78529.
RefSeqiNP_032106.1. NM_008080.5.
NP_082015.2. NM_027739.2.
XP_006513282.1. XM_006513219.1.
UniGeneiMm.386762.

Genome annotation databases

EnsembliENSMUST00000006914; ENSMUSP00000006914; ENSMUSG00000006731.
GeneIDi14421.
KEGGimmu:14421.
UCSCiuc007hic.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

Beta-1,4 N-acetylgalactosaminyltransferase 1

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L25885 mRNA. Translation: AAA65027.1 .
U18975 mRNA. Translation: AAA85496.1 .
BC036996 mRNA. Translation: AAH36996.1 .
BC057199 mRNA. Translation: AAH57199.1 .
CCDSi CCDS24230.1.
PIRi S78529.
RefSeqi NP_032106.1. NM_008080.5.
NP_082015.2. NM_027739.2.
XP_006513282.1. XM_006513219.1.
UniGenei Mm.386762.

3D structure databases

ProteinModelPortali Q09200.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000006914.

Protein family/group databases

CAZyi GT12. Glycosyltransferase Family 12.

PTM databases

PhosphoSitei Q09200.

Proteomic databases

MaxQBi Q09200.
PaxDbi Q09200.
PRIDEi Q09200.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000006914 ; ENSMUSP00000006914 ; ENSMUSG00000006731 .
GeneIDi 14421.
KEGGi mmu:14421.
UCSCi uc007hic.2. mouse.

Organism-specific databases

CTDi 2583.
MGIi MGI:1342057. B4galnt1.

Phylogenomic databases

eggNOGi NOG40821.
HOGENOMi HOG000220853.
HOVERGENi HBG004812.
InParanoidi Q09200.
KOi K00725.
OMAi IEHYLMP.
OrthoDBi EOG7R2BJS.
PhylomeDBi Q09200.
TreeFami TF332297.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Miscellaneous databases

NextBioi 286013.
PROi Q09200.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q09200.
Bgeei Q09200.
CleanExi MM_B4GALNT1.
Genevestigatori Q09200.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR001173. Glyco_trans_2-like.
IPR011143. GM2_synthase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
Pfami PF00535. Glycos_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000474. GM2_GD2_synthase. 1 hit.
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "T cell receptor-mediated stimulation of mouse thymocytes induces up-regulation of the GM2/GD2 synthase gene."
    Takamiya K., Yamamoto A., Yamashiro S., Furukawa K., Haraguchi M., Okada M., Ikeda T., Shiku H., Furukawa K.
    FEBS Lett. 358:79-83(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Beta-1,4-N-acetylgalactosaminyltransferase involved in ganglioside synthesis: cDNA sequence, expression, and chromosome mapping of the mouse gene."
    Sango K., Johnson O.N., Kozak C.A., Proia R.L.
    Genomics 27:362-365(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Neonatal brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: 129.
    Tissue: Mammary gland.

Entry informationi

Entry nameiB4GN1_MOUSE
AccessioniPrimary (citable) accession number: Q09200
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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