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Q09200 (B4GN1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 127. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-1,4 N-acetylgalactosaminyltransferase 1

EC=2.4.1.92
Alternative name(s):
(N-acetylneuraminyl)-galactosylglucosylceramide
GM2/GD2 synthase
GalNAc-T
Gene names
Name:B4galnt1
Synonyms:Galgt, Galgt1, Ggm2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length533 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the biosynthesis of gangliosides GM2, GD2 and GA2.

Catalytic activity

UDP-N-acetyl-alpha-D-galactosamine + O-(N-acetyl-alpha-neuraminyl)-(2->3)-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->1)-ceramide = UDP + O-2-(acetylamino)-2-deoxy-beta-D-galactopyranosyl-(1->4)-O-(N-acetyl-alpha-neuraminyl-(2->3))-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->1)-ceramide.

Pathway

Protein modification; protein glycosylation.

Subunit structure

Homodimer; disulfide-linked By similarity.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein.

Tissue specificity

Most abundant in brain, liver, lung, spleen and testis.

Developmental stage

Highest at day 7 of embryonic development after which it declines to its lowest levels at day 11 before increasing again.

Sequence similarities

Belongs to the glycosyltransferase 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 533533Beta-1,4 N-acetylgalactosaminyltransferase 1
PRO_0000059101

Regions

Topological domain1 – 77Cytoplasmic Potential
Transmembrane8 – 2518Helical; Signal-anchor for type II membrane protein; Potential
Topological domain26 – 533508Lumenal Potential

Amino acid modifications

Glycosylation791N-linked (GlcNAc...) Potential
Glycosylation1791N-linked (GlcNAc...) Potential
Glycosylation2741N-linked (GlcNAc...) Potential
Disulfide bond80Interchain (with C-412) By similarity
Disulfide bond82Interchain (with C-529) By similarity
Disulfide bond412Interchain (with C-80) By similarity
Disulfide bond429 ↔ 476 By similarity
Disulfide bond529Interchain (with C-82) By similarity

Experimental info

Sequence conflict731G → E in AAA85496. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q09200 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 6A15D81D2A68A28F

FASTA53359,212
        10         20         30         40         50         60 
MRLDRRALYA LVLLLACASL GLLYSSTRNA PSLPNPLALW SPPQGPPRLD LLDLAPEPRY 

        70         80         90        100        110        120 
AHIPVRIKEQ VVGLLAQNNC SCESKGGSLP LPFLRQVRAV DLTKAFDAEE LRAVSVAREQ 

       130        140        150        160        170        180 
EYQAFLARSR SLADQLLIAP ANSPLQYPLQ GVEVQPLRSI LVPGLSLQEA SVQEIYQVNL 

       190        200        210        220        230        240 
SASLGTWDVA GEVTGVTLTG EGQPDLTLAS PVLDKLNRQL QLVTYSSRSY QANTADTVRF 

       250        260        270        280        290        300 
STKGHEVAFT ILVRHPPNPR LYPPSSLPQG AEYNISALVT IATKTFLRYD RLRTLIASIR 

       310        320        330        340        350        360 
RFYPTVTIVI ADDSDKPERI SDPHVEHYFM PFGKGWFAGR NLAVSQVTTK YVLWVDDDFV 

       370        380        390        400        410        420 
FTARTRLEKL VDVLEKTPLD LVGGAVREIS GYATTYRQLL SVEPGAPGLG NCFRQKQGFH 

       430        440        450        460        470        480 
HELVGFPSCV VTDGVVNFFL ARTDKVRQVG FDPRLNRVAH LEFFLDGLGF LRVGSCSDVV 

       490        500        510        520        530 
VDHASKVKLP WTAKDPGAET YARYRYPGSL DQSQVAKHRL LFFKHRLQCM TAE 

« Hide

References

« Hide 'large scale' references
[1]"T cell receptor-mediated stimulation of mouse thymocytes induces up-regulation of the GM2/GD2 synthase gene."
Takamiya K., Yamamoto A., Yamashiro S., Furukawa K., Haraguchi M., Okada M., Ikeda T., Shiku H., Furukawa K.
FEBS Lett. 358:79-83(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Beta-1,4-N-acetylgalactosaminyltransferase involved in ganglioside synthesis: cDNA sequence, expression, and chromosome mapping of the mouse gene."
Sango K., Johnson O.N., Kozak C.A., Proia R.L.
Genomics 27:362-365(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Neonatal brain.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: 129.
Tissue: Mammary gland.
+Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

Beta-1,4 N-acetylgalactosaminyltransferase 1

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L25885 mRNA. Translation: AAA65027.1.
U18975 mRNA. Translation: AAA85496.1.
BC036996 mRNA. Translation: AAH36996.1.
BC057199 mRNA. Translation: AAH57199.1.
PIRS78529.
RefSeqNP_032106.1. NM_008080.5.
NP_082015.2. NM_027739.2.
XP_006513282.1. XM_006513219.1.
UniGeneMm.386762.

3D structure databases

ProteinModelPortalQ09200.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000006914.

Protein family/group databases

CAZyGT12. Glycosyltransferase Family 12.

PTM databases

PhosphoSiteQ09200.

Proteomic databases

PaxDbQ09200.
PRIDEQ09200.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000006914; ENSMUSP00000006914; ENSMUSG00000006731.
GeneID14421.
KEGGmmu:14421.
UCSCuc007hic.2. mouse.

Organism-specific databases

CTD2583.
MGIMGI:1342057. B4galnt1.

Phylogenomic databases

eggNOGNOG40821.
HOGENOMHOG000220853.
HOVERGENHBG004812.
InParanoidQ09200.
KOK00725.
OMAIEHYLMP.
OrthoDBEOG7R2BJS.
PhylomeDBQ09200.
TreeFamTF332297.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

ArrayExpressQ09200.
BgeeQ09200.
CleanExMM_B4GALNT1.
GenevestigatorQ09200.

Family and domain databases

InterProIPR001173. Glyco_trans_2-like.
IPR011143. GM2_synthase.
[Graphical view]
PfamPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
PIRSFPIRSF000474. GM2_GD2_synthase. 1 hit.
ProtoNetSearch...

Other

NextBio286013.
PROQ09200.
SOURCESearch...

Entry information

Entry nameB4GN1_MOUSE
AccessionPrimary (citable) accession number: Q09200
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot