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Protein

Beta-1,4 N-acetylgalactosaminyltransferase 1

Gene

B4galnt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the biosynthesis of gangliosides GM2, GD2 and GA2.

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + O-(N-acetyl-alpha-neuraminyl)-(2->3)-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->1)-ceramide = UDP + O-2-(acetylamino)-2-deoxy-beta-D-galactopyranosyl-(1->4)-O-(N-acetyl-alpha-neuraminyl-(2->3))-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->1)-ceramide.

Pathwayi

GO - Molecular functioni

  1. (N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. ganglioside biosynthetic process Source: MGI
  2. glycosphingolipid metabolic process Source: MGI
  3. lipid glycosylation Source: InterPro
  4. lipid storage Source: MGI
  5. protein glycosylation Source: UniProtKB-UniPathway
  6. spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT12. Glycosyltransferase Family 12.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4 N-acetylgalactosaminyltransferase 1 (EC:2.4.1.92)
Alternative name(s):
(N-acetylneuraminyl)-galactosylglucosylceramide
GM2/GD2 synthase
GalNAc-T
Gene namesi
Name:B4galnt1
Synonyms:Galgt, Galgt1, Ggm2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:1342057. B4galnt1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence Analysis
Transmembranei8 – 2518Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini26 – 533508LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of Golgi membrane Source: InterPro
  2. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 533533Beta-1,4 N-acetylgalactosaminyltransferase 1PRO_0000059101Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi80 – 80Interchain (with C-412)By similarity
Disulfide bondi82 – 82Interchain (with C-529)By similarity
Glycosylationi179 – 1791N-linked (GlcNAc...)Sequence Analysis
Glycosylationi274 – 2741N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi412 – 412Interchain (with C-80)By similarity
Disulfide bondi429 ↔ 476By similarity
Disulfide bondi529 – 529Interchain (with C-82)By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ09200.
PaxDbiQ09200.
PRIDEiQ09200.

PTM databases

PhosphoSiteiQ09200.

Expressioni

Tissue specificityi

Most abundant in brain, liver, lung, spleen and testis.

Developmental stagei

Highest at day 7 of embryonic development after which it declines to its lowest levels at day 11 before increasing again.

Gene expression databases

BgeeiQ09200.
CleanExiMM_B4GALNT1.
ExpressionAtlasiQ09200. baseline and differential.
GenevestigatoriQ09200.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000006914.

Structurei

3D structure databases

ProteinModelPortaliQ09200.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG40821.
HOGENOMiHOG000220853.
HOVERGENiHBG004812.
InParanoidiQ09200.
KOiK00725.
OMAiIEHYLMP.
OrthoDBiEOG7R2BJS.
PhylomeDBiQ09200.
TreeFamiTF332297.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR011143. GM2_synthase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000474. GM2_GD2_synthase. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q09200-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRLDRRALYA LVLLLACASL GLLYSSTRNA PSLPNPLALW SPPQGPPRLD
60 70 80 90 100
LLDLAPEPRY AHIPVRIKEQ VVGLLAQNNC SCESKGGSLP LPFLRQVRAV
110 120 130 140 150
DLTKAFDAEE LRAVSVAREQ EYQAFLARSR SLADQLLIAP ANSPLQYPLQ
160 170 180 190 200
GVEVQPLRSI LVPGLSLQEA SVQEIYQVNL SASLGTWDVA GEVTGVTLTG
210 220 230 240 250
EGQPDLTLAS PVLDKLNRQL QLVTYSSRSY QANTADTVRF STKGHEVAFT
260 270 280 290 300
ILVRHPPNPR LYPPSSLPQG AEYNISALVT IATKTFLRYD RLRTLIASIR
310 320 330 340 350
RFYPTVTIVI ADDSDKPERI SDPHVEHYFM PFGKGWFAGR NLAVSQVTTK
360 370 380 390 400
YVLWVDDDFV FTARTRLEKL VDVLEKTPLD LVGGAVREIS GYATTYRQLL
410 420 430 440 450
SVEPGAPGLG NCFRQKQGFH HELVGFPSCV VTDGVVNFFL ARTDKVRQVG
460 470 480 490 500
FDPRLNRVAH LEFFLDGLGF LRVGSCSDVV VDHASKVKLP WTAKDPGAET
510 520 530
YARYRYPGSL DQSQVAKHRL LFFKHRLQCM TAE
Length:533
Mass (Da):59,212
Last modified:November 1, 1995 - v1
Checksum:i6A15D81D2A68A28F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731G → E in AAA85496. (PubMed:7558008)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25885 mRNA. Translation: AAA65027.1.
U18975 mRNA. Translation: AAA85496.1.
BC036996 mRNA. Translation: AAH36996.1.
BC057199 mRNA. Translation: AAH57199.1.
CCDSiCCDS24230.1.
PIRiS78529.
RefSeqiNP_032106.1. NM_008080.5.
NP_082015.2. NM_027739.2.
XP_006513282.1. XM_006513219.1.
UniGeneiMm.386762.

Genome annotation databases

EnsembliENSMUST00000006914; ENSMUSP00000006914; ENSMUSG00000006731.
GeneIDi14421.
KEGGimmu:14421.
UCSCiuc007hic.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

Beta-1,4 N-acetylgalactosaminyltransferase 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25885 mRNA. Translation: AAA65027.1.
U18975 mRNA. Translation: AAA85496.1.
BC036996 mRNA. Translation: AAH36996.1.
BC057199 mRNA. Translation: AAH57199.1.
CCDSiCCDS24230.1.
PIRiS78529.
RefSeqiNP_032106.1. NM_008080.5.
NP_082015.2. NM_027739.2.
XP_006513282.1. XM_006513219.1.
UniGeneiMm.386762.

3D structure databases

ProteinModelPortaliQ09200.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000006914.

Protein family/group databases

CAZyiGT12. Glycosyltransferase Family 12.

PTM databases

PhosphoSiteiQ09200.

Proteomic databases

MaxQBiQ09200.
PaxDbiQ09200.
PRIDEiQ09200.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006914; ENSMUSP00000006914; ENSMUSG00000006731.
GeneIDi14421.
KEGGimmu:14421.
UCSCiuc007hic.2. mouse.

Organism-specific databases

CTDi2583.
MGIiMGI:1342057. B4galnt1.

Phylogenomic databases

eggNOGiNOG40821.
HOGENOMiHOG000220853.
HOVERGENiHBG004812.
InParanoidiQ09200.
KOiK00725.
OMAiIEHYLMP.
OrthoDBiEOG7R2BJS.
PhylomeDBiQ09200.
TreeFamiTF332297.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

NextBioi286013.
PROiQ09200.
SOURCEiSearch...

Gene expression databases

BgeeiQ09200.
CleanExiMM_B4GALNT1.
ExpressionAtlasiQ09200. baseline and differential.
GenevestigatoriQ09200.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR011143. GM2_synthase.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000474. GM2_GD2_synthase. 1 hit.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "T cell receptor-mediated stimulation of mouse thymocytes induces up-regulation of the GM2/GD2 synthase gene."
    Takamiya K., Yamamoto A., Yamashiro S., Furukawa K., Haraguchi M., Okada M., Ikeda T., Shiku H., Furukawa K.
    FEBS Lett. 358:79-83(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Beta-1,4-N-acetylgalactosaminyltransferase involved in ganglioside synthesis: cDNA sequence, expression, and chromosome mapping of the mouse gene."
    Sango K., Johnson O.N., Kozak C.A., Proia R.L.
    Genomics 27:362-365(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Neonatal brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: 129.
    Tissue: Mammary gland.

Entry informationi

Entry nameiB4GN1_MOUSE
AccessioniPrimary (citable) accession number: Q09200
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.