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Q09200

- B4GN1_MOUSE

UniProt

Q09200 - B4GN1_MOUSE

Protein

Beta-1,4 N-acetylgalactosaminyltransferase 1

Gene

B4galnt1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Involved in the biosynthesis of gangliosides GM2, GD2 and GA2.

    Catalytic activityi

    UDP-N-acetyl-alpha-D-galactosamine + O-(N-acetyl-alpha-neuraminyl)-(2->3)-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->1)-ceramide = UDP + O-2-(acetylamino)-2-deoxy-beta-D-galactopyranosyl-(1->4)-O-(N-acetyl-alpha-neuraminyl-(2->3))-O-beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1<->1)-ceramide.

    Pathwayi

    GO - Molecular functioni

    1. (N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. ganglioside biosynthetic process Source: MGI
    2. glycosphingolipid metabolic process Source: MGI
    3. lipid glycosylation Source: InterPro
    4. lipid storage Source: MGI
    5. protein glycosylation Source: UniProtKB-UniPathway
    6. spermatogenesis Source: MGI

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT12. Glycosyltransferase Family 12.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,4 N-acetylgalactosaminyltransferase 1 (EC:2.4.1.92)
    Alternative name(s):
    (N-acetylneuraminyl)-galactosylglucosylceramide
    GM2/GD2 synthase
    GalNAc-T
    Gene namesi
    Name:B4galnt1
    Synonyms:Galgt, Galgt1, Ggm2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:1342057. B4galnt1.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of Golgi membrane Source: InterPro
    2. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 533533Beta-1,4 N-acetylgalactosaminyltransferase 1PRO_0000059101Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi80 – 80Interchain (with C-412)By similarity
    Disulfide bondi82 – 82Interchain (with C-529)By similarity
    Glycosylationi179 – 1791N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi274 – 2741N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi412 – 412Interchain (with C-80)By similarity
    Disulfide bondi429 ↔ 476By similarity
    Disulfide bondi529 – 529Interchain (with C-82)By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiQ09200.
    PaxDbiQ09200.
    PRIDEiQ09200.

    PTM databases

    PhosphoSiteiQ09200.

    Expressioni

    Tissue specificityi

    Most abundant in brain, liver, lung, spleen and testis.

    Developmental stagei

    Highest at day 7 of embryonic development after which it declines to its lowest levels at day 11 before increasing again.

    Gene expression databases

    ArrayExpressiQ09200.
    BgeeiQ09200.
    CleanExiMM_B4GALNT1.
    GenevestigatoriQ09200.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.By similarity

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000006914.

    Structurei

    3D structure databases

    ProteinModelPortaliQ09200.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 77CytoplasmicSequence Analysis
    Topological domaini26 – 533508LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei8 – 2518Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 2 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG40821.
    HOGENOMiHOG000220853.
    HOVERGENiHBG004812.
    InParanoidiQ09200.
    KOiK00725.
    OMAiIEHYLMP.
    OrthoDBiEOG7R2BJS.
    PhylomeDBiQ09200.
    TreeFamiTF332297.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR001173. Glyco_trans_2-like.
    IPR011143. GM2_synthase.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PfamiPF00535. Glycos_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000474. GM2_GD2_synthase. 1 hit.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q09200-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLDRRALYA LVLLLACASL GLLYSSTRNA PSLPNPLALW SPPQGPPRLD    50
    LLDLAPEPRY AHIPVRIKEQ VVGLLAQNNC SCESKGGSLP LPFLRQVRAV 100
    DLTKAFDAEE LRAVSVAREQ EYQAFLARSR SLADQLLIAP ANSPLQYPLQ 150
    GVEVQPLRSI LVPGLSLQEA SVQEIYQVNL SASLGTWDVA GEVTGVTLTG 200
    EGQPDLTLAS PVLDKLNRQL QLVTYSSRSY QANTADTVRF STKGHEVAFT 250
    ILVRHPPNPR LYPPSSLPQG AEYNISALVT IATKTFLRYD RLRTLIASIR 300
    RFYPTVTIVI ADDSDKPERI SDPHVEHYFM PFGKGWFAGR NLAVSQVTTK 350
    YVLWVDDDFV FTARTRLEKL VDVLEKTPLD LVGGAVREIS GYATTYRQLL 400
    SVEPGAPGLG NCFRQKQGFH HELVGFPSCV VTDGVVNFFL ARTDKVRQVG 450
    FDPRLNRVAH LEFFLDGLGF LRVGSCSDVV VDHASKVKLP WTAKDPGAET 500
    YARYRYPGSL DQSQVAKHRL LFFKHRLQCM TAE 533
    Length:533
    Mass (Da):59,212
    Last modified:November 1, 1995 - v1
    Checksum:i6A15D81D2A68A28F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti73 – 731G → E in AAA85496. (PubMed:7558008)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L25885 mRNA. Translation: AAA65027.1.
    U18975 mRNA. Translation: AAA85496.1.
    BC036996 mRNA. Translation: AAH36996.1.
    BC057199 mRNA. Translation: AAH57199.1.
    CCDSiCCDS24230.1.
    PIRiS78529.
    RefSeqiNP_032106.1. NM_008080.5.
    NP_082015.2. NM_027739.2.
    XP_006513282.1. XM_006513219.1.
    UniGeneiMm.386762.

    Genome annotation databases

    EnsembliENSMUST00000006914; ENSMUSP00000006914; ENSMUSG00000006731.
    GeneIDi14421.
    KEGGimmu:14421.
    UCSCiuc007hic.2. mouse.

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    Beta-1,4 N-acetylgalactosaminyltransferase 1

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L25885 mRNA. Translation: AAA65027.1 .
    U18975 mRNA. Translation: AAA85496.1 .
    BC036996 mRNA. Translation: AAH36996.1 .
    BC057199 mRNA. Translation: AAH57199.1 .
    CCDSi CCDS24230.1.
    PIRi S78529.
    RefSeqi NP_032106.1. NM_008080.5.
    NP_082015.2. NM_027739.2.
    XP_006513282.1. XM_006513219.1.
    UniGenei Mm.386762.

    3D structure databases

    ProteinModelPortali Q09200.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000006914.

    Protein family/group databases

    CAZyi GT12. Glycosyltransferase Family 12.

    PTM databases

    PhosphoSitei Q09200.

    Proteomic databases

    MaxQBi Q09200.
    PaxDbi Q09200.
    PRIDEi Q09200.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000006914 ; ENSMUSP00000006914 ; ENSMUSG00000006731 .
    GeneIDi 14421.
    KEGGi mmu:14421.
    UCSCi uc007hic.2. mouse.

    Organism-specific databases

    CTDi 2583.
    MGIi MGI:1342057. B4galnt1.

    Phylogenomic databases

    eggNOGi NOG40821.
    HOGENOMi HOG000220853.
    HOVERGENi HBG004812.
    InParanoidi Q09200.
    KOi K00725.
    OMAi IEHYLMP.
    OrthoDBi EOG7R2BJS.
    PhylomeDBi Q09200.
    TreeFami TF332297.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    NextBioi 286013.
    PROi Q09200.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q09200.
    Bgeei Q09200.
    CleanExi MM_B4GALNT1.
    Genevestigatori Q09200.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR001173. Glyco_trans_2-like.
    IPR011143. GM2_synthase.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    Pfami PF00535. Glycos_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000474. GM2_GD2_synthase. 1 hit.
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "T cell receptor-mediated stimulation of mouse thymocytes induces up-regulation of the GM2/GD2 synthase gene."
      Takamiya K., Yamamoto A., Yamashiro S., Furukawa K., Haraguchi M., Okada M., Ikeda T., Shiku H., Furukawa K.
      FEBS Lett. 358:79-83(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Beta-1,4-N-acetylgalactosaminyltransferase involved in ganglioside synthesis: cDNA sequence, expression, and chromosome mapping of the mouse gene."
      Sango K., Johnson O.N., Kozak C.A., Proia R.L.
      Genomics 27:362-365(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Neonatal brain.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: 129.
      Tissue: Mammary gland.

    Entry informationi

    Entry nameiB4GN1_MOUSE
    AccessioniPrimary (citable) accession number: Q09200
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3