Q09199 (B4GN2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-1,4 N-acetylgalactosaminyltransferase 2
EC=2.4.1.-

Gene names

Name:	B4galnt2
Synonyms:	Galgt2, Ggm3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Responsible for synthesis of murine T-lymphocyte CT antigen. Can add to the low molecular weight acceptor 3'-sialyl-N-acetyllactosamine, to form a non-reducing terminal tetrasaccharide structure.
Catalytic activity	UDP-N-acetyl-D-galactosamine + (N-acetylneuraminyl)-D-galactosyl-D-R = UDP + N-acetyl-D-galactosaminyl-(N-acetylneuraminyl)-D-galactosyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the glycosyltransferase 2 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	UDP-N-acetylgalactosamine metabolic process Inferred from sequence or structural similarity. Source: UniProtKB UDP-N-acetylglucosamine metabolic process Inferred from mutant phenotype. Source: MGI lipid glycosylation Inferred from electronic annotation. Source: InterPro negative regulation of cell-cell adhesion Inferred from sequence or structural similarity. Source: UniProtKB protein glycosylation Inferred from mutant phenotype. Source: MGI
Cellular component	integral to Golgi membrane Inferred from electronic annotation. Source: InterPro microsome Inferred from direct assay Ref.1. Source: MGI
Molecular function	acetylgalactosaminyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 510	510	Beta-1,4 N-acetylgalactosaminyltransferase 2		PRO_0000059104
Regions
	Topological domain	1 – 15	15	Cytoplasmic Potential
	Transmembrane	16 – 32	17	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	33 – 510	478	Lumenal Potential
Amino acid modifications
	Glycosylation	390	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q09199 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 013783C80ADEC3CB	FASTA	510	58,317
	10 20 30 40 50 60 MTSSVSFASF RFPWLLKTFV LMVGLATVAF MVRKVSLTTD FSTFKPKFPE PARVDPVLKL 70 80 90 100 110 120 LPEEHLRKLF TYSDIWLFPK NQCDCNSGKL RMKYKFQDAY NQKDLPAVNA RRQAEFEHFQ 130 140 150 160 170 180 RREGLPRPPP LLAPPNLPFG YPVHGVEVMP LHTILIPGLQ YEGPDAPVYE VILKASLGTL 190 200 210 220 230 240 NTLADVPDDE VQGRGQRQLT ISTRHRKVLN FILQHVTYTS TEYYLHKVDT VSMEYESSVA 250 260 270 280 290 300 KFPVTIKQQT VPKLYDPGPE RKIRNLVTIA TKTFLRPHKL KILLQSIRKY YPDITVIVAD 310 320 330 340 350 360 DSKEPLEIND DYVEYYTMPF GKGWFAGRNL AISQVTTKYV LWVDDDFLFS DKTKIEVLVD 370 380 390 400 410 420 VLEKTELDVV GGSVQGNTYQ FRLLYEQTKN GSCLHQRWGS FQALDGFPGC TLTSGVVNFF 430 440 450 460 470 480 LAHTEQLRRV GFDPILQRVA HGEFFIDGLG RLLVGSCPGV IINHQVRTPP KDPKLAALEK 490 500 510 TYDKYRANTN SVIQFKVALQ YFKNHLYCST

References

[1]	"Molecular cloning of a murine N-acetylgalactosamine transferase cDNA that determines expression of the T lymphocyte-specific CT oligosaccharide differentiation antigen." Smith P.L., Lowe J.B. J. Biol. Chem. 269:15162-15171(1994) [PubMed: 7515051] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L30104 mRNA. Translation: AAA39802.1.
IPI	IPI00121825.
PIR	A53802.
RefSeq	NP_032107.1. NM_008081.3.
UniGene	Mm.340702.
3D structure databases
ProteinModelPortal	Q09199.
ModBase	Search...
Protein-protein interaction databases
STRING	Q09199.
Protein family/group databases
CAZy	GT12. Glycosyltransferase Family 12.
PTM databases
PhosphoSite	Q09199.
Proteomic databases
PRIDE	Q09199.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000038343; ENSMUSP00000037239; ENSMUSG00000013418.
GeneID	14422.
KEGG	mmu:14422.
Organism-specific databases
CTD	124872.
MGI	MGI:1342058. B4galnt2.
Phylogenomic databases
HOGENOM	HBG445842.
HOVERGEN	HBG004812.
InParanoid	Q09199.
OrthoDB	EOG4SQWWF.
PhylomeDB	Q09199.
Gene expression databases
ArrayExpress	Q09199.
Bgee	Q09199.
CleanEx	MM_B4GALNT2.
Genevestigator	Q09199.
GermOnline	ENSMUSG00000013418. Mus musculus.
Family and domain databases
InterPro	IPR001173. Glyco_trans_2. IPR011143. GM2_synthase. [Graphical view]
KO	K09655.
Pfam	PF00535. Glycos_transf_2. 1 hit. [Graphical view]
PIRSF	PIRSF000474. GM2_GD2_synthase. 1 hit.
ProtoNet	Search...
Other
NextBio	286017.
SOURCE	Search...

Entry information

Entry name

B4GN2_MOUSE

Accession

Primary (citable) accession number: Q09199

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 16, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program