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Protein

Myosin regulatory light chain cdc4

Gene

cdc4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in cytokinesis. Required for the formation and function of the contractile ring.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi87 – 9812PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • barrier septum assembly Source: UniProtKB-KW
  • mitotic actomyosin contractile ring assembly Source: PomBase
  • mitotic actomyosin contractile ring assembly actin filament organization Source: PomBase
  • protein localization to medial cortex Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Biological processi

Cell cycle, Cell division, Septation

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-SPO-5627123. RHO GTPases activate PAKs.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin regulatory light chain cdc4
Gene namesi
Name:cdc4
ORF Names:SPAP8A3.08
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAP8A3.08.
PomBaseiSPAP8A3.08. cdc4.

Subcellular locationi

GO - Cellular componenti

  • actomyosin contractile ring Source: PomBase
  • cell division site Source: PomBase
  • cytosol Source: PomBase
  • medial cortical node Source: PomBase
  • myosin II complex Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 141141Myosin regulatory light chain cdc4PRO_0000198763Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21Phosphoserine1 Publication
Modified residuei6 – 61Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated on either Ser-2 or Ser-6 but not both. Phosphorylation is not essential for the function of the protein.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ09196.

PTM databases

iPTMnetiQ09196.

Interactioni

Subunit structurei

Binds to myosin II chains myo2 and myo3. Interacts with vps27 and a PI 4-kinase pik1.1 Publication

Protein-protein interaction databases

BioGridi278195. 31 interactions.
MINTiMINT-151117.

Structurei

Secondary structure

1
141
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni6 – 83Combined sources
Helixi9 – 146Combined sources
Beta strandi17 – 237Combined sources
Helixi25 – 3410Combined sources
Helixi41 – 488Combined sources
Beta strandi53 – 564Combined sources
Helixi57 – 648Combined sources
Beta strandi68 – 736Combined sources
Helixi76 – 849Combined sources
Helixi96 – 10712Combined sources
Helixi112 – 12110Combined sources
Turni130 – 1323Combined sources
Helixi133 – 1397Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GGWNMR-A2-141[»]
ProteinModelPortaliQ09196.
SMRiQ09196. Positions 2-141.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ09196.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 3836EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini74 – 10936EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini109 – 14133EF-hand 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000233018.
InParanoidiQ09196.
OMAiYEGTITH.
OrthoDBiEOG7PK99H.
PhylomeDBiQ09196.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09196-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTDDSPYKQ AFSLFDRHGT GRIPKTSIGD LLRACGQNPT LAEITEIEST
60 70 80 90 100
LPAEVDMEQF LQVLNRPNGF DMPGDPEEFV KGFQVFDKDA TGMIGVGELR
110 120 130 140
YVLTSLGEKL SNEEMDELLK GVPVKDGMVN YHDFVQMILA N
Length:141
Mass (Da):15,661
Last modified:November 1, 1996 - v1
Checksum:i691731DD7C6A68A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42454 Genomic DNA. Translation: AAA67467.1.
CU329670 Genomic DNA. Translation: CAB55175.1.
PIRiT39245.
RefSeqiNP_594947.1. NM_001020378.2.

Genome annotation databases

EnsemblFungiiSPAP8A3.08.1; SPAP8A3.08.1:pep; SPAP8A3.08.
GeneIDi2541699.
KEGGispo:SPAP8A3.08.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42454 Genomic DNA. Translation: AAA67467.1.
CU329670 Genomic DNA. Translation: CAB55175.1.
PIRiT39245.
RefSeqiNP_594947.1. NM_001020378.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GGWNMR-A2-141[»]
ProteinModelPortaliQ09196.
SMRiQ09196. Positions 2-141.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278195. 31 interactions.
MINTiMINT-151117.

PTM databases

iPTMnetiQ09196.

Proteomic databases

MaxQBiQ09196.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAP8A3.08.1; SPAP8A3.08.1:pep; SPAP8A3.08.
GeneIDi2541699.
KEGGispo:SPAP8A3.08.

Organism-specific databases

EuPathDBiFungiDB:SPAP8A3.08.
PomBaseiSPAP8A3.08. cdc4.

Phylogenomic databases

HOGENOMiHOG000233018.
InParanoidiQ09196.
OMAiYEGTITH.
OrthoDBiEOG7PK99H.
PhylomeDBiQ09196.

Enzyme and pathway databases

ReactomeiR-SPO-5627123. RHO GTPases activate PAKs.

Miscellaneous databases

EvolutionaryTraceiQ09196.
NextBioi20802792.
PROiQ09196.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Schizosaccharomyces pombe cdc4+ gene encodes a novel EF-hand protein essential for cytokinesis."
    McCollum D., Balasubramanian M.K., Pelcher L.E., Hemmingsen S.M., Gould K.L.
    J. Cell Biol. 130:651-660(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Phosphorylation of the myosin-II light chain does not regulate the timing of cytokinesis in fission yeast."
    McCollum D., Feoktistova A., Gould K.L.
    J. Biol. Chem. 274:17691-17695(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.
  4. "Cdc4p, a contractile ring protein essential for cytokinesis in Schizosaccharomyces pombe, interacts with a phosphatidylinositol 4-kinase."
    Desautels M., Den Haese J.P., Slupsky C.M., McIntosh L.P., Hemmingsen S.M.
    J. Biol. Chem. 276:5932-5942(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION.
  5. "Structure of Cdc4p, a contractile ring protein essential for cytokinesis in Schizosaccharomyces pombe."
    Slupsky C.M., Desautels M., Huebert T., Zhao R., Hemmingsen S.M., McIntosh L.P.
    J. Biol. Chem. 276:5943-5951(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR.

Entry informationi

Entry nameiMLR4_SCHPO
AccessioniPrimary (citable) accession number: Q09196
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In vitro, this chain does not seem to bind calcium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.