Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Myosin regulatory light chain cdc4

Gene

cdc4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in cytokinesis. Required for the formation and function of the contractile ring.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi87 – 98PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • barrier septum assembly Source: UniProtKB-KW
  • mitotic actomyosin contractile ring assembly Source: PomBase
  • mitotic actomyosin contractile ring assembly actin filament organization Source: PomBase
  • protein localization to medial cortex Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Myosin

Keywords - Biological processi

Cell cycle, Cell division, Septation

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-SPO-5627123. RHO GTPases activate PAKs.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin regulatory light chain cdc4
Gene namesi
Name:cdc4
ORF Names:SPAP8A3.08
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAP8A3.08.
PomBaseiSPAP8A3.08. cdc4.

Subcellular locationi

GO - Cellular componenti

  • actomyosin contractile ring Source: PomBase
  • cell division site Source: PomBase
  • cytosol Source: PomBase
  • medial cortical node Source: PomBase
  • myosin II complex Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001987631 – 141Myosin regulatory light chain cdc4Add BLAST141

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2Phosphoserine1 Publication1
Modified residuei6Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated on either Ser-2 or Ser-6 but not both. Phosphorylation is not essential for the function of the protein.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ09196.
PRIDEiQ09196.

PTM databases

iPTMnetiQ09196.

Interactioni

Subunit structurei

Binds to myosin II chains myo2 and myo3. Interacts with vps27 and a PI 4-kinase pik1.1 Publication

Protein-protein interaction databases

BioGridi278195. 31 interactors.
MINTiMINT-151117.

Structurei

Secondary structure

1141
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni6 – 8Combined sources3
Helixi9 – 14Combined sources6
Beta strandi17 – 23Combined sources7
Helixi25 – 34Combined sources10
Helixi41 – 48Combined sources8
Beta strandi53 – 56Combined sources4
Helixi57 – 64Combined sources8
Beta strandi68 – 73Combined sources6
Helixi76 – 84Combined sources9
Helixi96 – 107Combined sources12
Helixi112 – 121Combined sources10
Turni130 – 132Combined sources3
Helixi133 – 139Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GGWNMR-A2-141[»]
ProteinModelPortaliQ09196.
SMRiQ09196.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ09196.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 38EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini74 – 109EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini109 – 141EF-hand 3PROSITE-ProRule annotationAdd BLAST33

Sequence similaritiesi

Contains 3 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000233018.
InParanoidiQ09196.
OMAiQEDANGH.
OrthoDBiEOG092C4XFN.
PhylomeDBiQ09196.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09196-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTDDSPYKQ AFSLFDRHGT GRIPKTSIGD LLRACGQNPT LAEITEIEST
60 70 80 90 100
LPAEVDMEQF LQVLNRPNGF DMPGDPEEFV KGFQVFDKDA TGMIGVGELR
110 120 130 140
YVLTSLGEKL SNEEMDELLK GVPVKDGMVN YHDFVQMILA N
Length:141
Mass (Da):15,661
Last modified:November 1, 1996 - v1
Checksum:i691731DD7C6A68A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42454 Genomic DNA. Translation: AAA67467.1.
CU329670 Genomic DNA. Translation: CAB55175.1.
PIRiT39245.
RefSeqiNP_594947.1. NM_001020378.2.

Genome annotation databases

EnsemblFungiiSPAP8A3.08.1; SPAP8A3.08.1:pep; SPAP8A3.08.
GeneIDi2541699.
KEGGispo:SPAP8A3.08.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42454 Genomic DNA. Translation: AAA67467.1.
CU329670 Genomic DNA. Translation: CAB55175.1.
PIRiT39245.
RefSeqiNP_594947.1. NM_001020378.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GGWNMR-A2-141[»]
ProteinModelPortaliQ09196.
SMRiQ09196.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278195. 31 interactors.
MINTiMINT-151117.

PTM databases

iPTMnetiQ09196.

Proteomic databases

MaxQBiQ09196.
PRIDEiQ09196.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAP8A3.08.1; SPAP8A3.08.1:pep; SPAP8A3.08.
GeneIDi2541699.
KEGGispo:SPAP8A3.08.

Organism-specific databases

EuPathDBiFungiDB:SPAP8A3.08.
PomBaseiSPAP8A3.08. cdc4.

Phylogenomic databases

HOGENOMiHOG000233018.
InParanoidiQ09196.
OMAiQEDANGH.
OrthoDBiEOG092C4XFN.
PhylomeDBiQ09196.

Enzyme and pathway databases

ReactomeiR-SPO-5627123. RHO GTPases activate PAKs.

Miscellaneous databases

EvolutionaryTraceiQ09196.
PROiQ09196.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMLR4_SCHPO
AccessioniPrimary (citable) accession number: Q09196
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

In vitro, this chain does not seem to bind calcium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.