ID   ERG24_SCHPO             Reviewed;         424 AA.
AC   Q09195;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 64.
DE   RecName: Full=Delta(14)-sterol reductase;
DE            EC=1.3.1.70;
DE   AltName: Full=C-14 sterol reductase;
DE   AltName: Full=Sterol C14-reductase;
GN   Name=erg24; ORFNames=SPBC16G5.18;
OS   Schizosaccharomyces pombe (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales;
OC   Schizosaccharomycetaceae; Schizosaccharomyces.
OX   NCBI_TaxID=4896;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=SP66;
RX   MEDLINE=95212923; PubMed=7698661; DOI=10.1016/0378-1119(94)00902-5;
RA   Smith S.;
RT   "Cloning and sequence analysis of an ERG24 homolog from
RT   Schizosaccharomyces pombe.";
RL   Gene 155:139-140(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 38366 / 972;
RX   MEDLINE=21848401; PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M.,
RA   Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A.,
RA   Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G.,
RA   Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K.,
RA   James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J.,
RA   Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C.,
RA   Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E.,
RA   Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S.,
RA   Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K.,
RA   Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S.,
RA   Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B.,
RA   Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D.,
RA   Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R.,
RA   Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B.,
RA   Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S.,
RA   Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M.,
RA   Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G.,
RA   Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J.,
RA   Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L.,
RA   Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J.,
RA   Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- FUNCTION: Reduces the C14=C15 double bond of 4,4-dimethyl-
CC       cholesta-8,14,24-trienol to produce 4,4-dimethyl-cholesta-8,24-
CC       dienol (By similarity).
CC   -!- CATALYTIC ACTIVITY: 4,4-dimethyl-5-alpha-cholesta-8,24-dien-3-
CC       beta-ol + NADP(+) = 4,4-dimethyl-5-alpha-cholesta-8,14,24-trien-3-
CC       beta-ol + NADPH.
CC   -!- PATHWAY: Steroid biosynthesis; zymosterol biosynthesis; zymosterol
CC       from lanosterol: step 2/6.
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein
CC       (Probable).
CC   -!- SIMILARITY: Belongs to the ERG4/ERG24 family.
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DR   EMBL; L36039; AAA74121.1; -; mRNA.
DR   EMBL; CU329671; CAA19037.1; -; Genomic_DNA.
DR   PIR; JC4057; JC4057.
DR   RefSeq; NP_596767.1; -.
DR   GeneID; 2540113; -.
DR   KEGG; spo:SPBC16G5.18; -.
DR   NMPDR; fig|4896.1.peg.2633; -.
DR   GeneDB_Spombe; SPBC16G5.18; -.
DR   OMA; Q09195; TMFHLLL.
DR   BioCyc; SPOM-XXX-01:SPOM-XXX-01-004750-MON; -.
DR   BRENDA; 1.3.1.70; 653.
DR   ArrayExpress; Q09195; -.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:GeneDB_SPombe.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0050613; F:delta14-sterol reductase activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0016126; P:sterol biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001171; Ergosterol_biosynth_ERG4_ERG24.
DR   InterPro; IPR018083; Sterol_reductase_CS.
DR   Pfam; PF01222; ERG4_ERG24; 1.
DR   PROSITE; PS01017; STEROL_REDUCT_1; 1.
DR   PROSITE; PS01018; STEROL_REDUCT_2; 1.
PE   2: Evidence at transcript level;
KW   Complete proteome; Lipid synthesis; Membrane; NADP; Oxidoreductase;
KW   Steroid biosynthesis; Sterol biosynthesis; Transmembrane.
FT   CHAIN         1    424       Delta(14)-sterol reductase.
FT                                /FTId=PRO_0000207493.
FT   TRANSMEM     19     39       Potential.
FT   TRANSMEM    112    132       Potential.
FT   TRANSMEM    370    390       Potential.
SQ   SEQUENCE   424 AA;  48560 MW;  1D3CE704F1E5EF5B CRC64;
     MAKGAVKKEK FEYEFFGPIG ALGVTVLTTV VSFGSFYICN EEGCPAKFSK ISHIFKKTPL
     FDQKSLILYL LWFSTLTLLW KCTNGKWAKG TPIDDKGTRL LYKINGFNSA CLILGVVCTS
     IYLLGASCME FIWDNFLQLM FAAYVFSVVL CTFCYVQSFF GKQQLAKGGT SGNILFDWFI
     GRSLNPRIGN FDIKCFCELR PGLILWVVFD IAFACHQYLV LGGRITDSMV LVIIFHTWYV
     LDSLINESAV LTTMDITTDG FGYMLSFGDL VWVPFLYSLQ ARYLAFHPVD LGLVKTLAIL
     CLQFLGYYIF RGANGQKNRF RSNPNDPKLK HLKFIQTKRG TKLLTSGWWG MARHINYFGD
     WIMAWAWCLP AGFGSPIPYF YVAYFGVLLV HRNARDDHKC RVKYGEDWEK YCKAVKYRII
     PYVY
//