Q09195 (ERG24_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Delta(14)-sterol reductase
EC=1.3.1.70

Alternative name(s):
C-14 sterol reductase
Sterol C14-reductase

Gene names

Name:	erg24
ORF Names:	SPBC16G5.18

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Reduces the C14=C15 double bond of 4,4-dimethyl-cholesta-8,14,24-trienol to produce 4,4-dimethyl-cholesta-8,24-dienol By similarity.
Catalytic activity	4,4-dimethyl-5-alpha-cholesta-8,24-dien-3-beta-ol + NADP+ = 4,4-dimethyl-5-alpha-cholesta-8,14,24-trien-3-beta-ol + NADPH.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 2/6.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the ERG4/ERG24 family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Steroid biosynthesis Steroid metabolism Sterol biosynthesis Sterol metabolism
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ergosterol biosynthetic process Inferred from sequence or structural similarity. Source: PomBase
Cellular_component	endoplasmic reticulum membrane Inferred from sequence or structural similarity. Source: PomBase integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	delta14-sterol reductase activity Inferred from sequence or structural similarity. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 424	424	Delta(14)-sterol reductase		PRO_0000207493
Regions
	Transmembrane	19 – 39	21	Helical; Potential
	Transmembrane	112 – 132	21	Helical; Potential
	Transmembrane	370 – 390	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q09195 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1D3CE704F1E5EF5B	FASTA	424	48,560
	10 20 30 40 50 60 MAKGAVKKEK FEYEFFGPIG ALGVTVLTTV VSFGSFYICN EEGCPAKFSK ISHIFKKTPL 70 80 90 100 110 120 FDQKSLILYL LWFSTLTLLW KCTNGKWAKG TPIDDKGTRL LYKINGFNSA CLILGVVCTS 130 140 150 160 170 180 IYLLGASCME FIWDNFLQLM FAAYVFSVVL CTFCYVQSFF GKQQLAKGGT SGNILFDWFI 190 200 210 220 230 240 GRSLNPRIGN FDIKCFCELR PGLILWVVFD IAFACHQYLV LGGRITDSMV LVIIFHTWYV 250 260 270 280 290 300 LDSLINESAV LTTMDITTDG FGYMLSFGDL VWVPFLYSLQ ARYLAFHPVD LGLVKTLAIL 310 320 330 340 350 360 CLQFLGYYIF RGANGQKNRF RSNPNDPKLK HLKFIQTKRG TKLLTSGWWG MARHINYFGD 370 380 390 400 410 420 WIMAWAWCLP AGFGSPIPYF YVAYFGVLLV HRNARDDHKC RVKYGEDWEK YCKAVKYRII PYVY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequence analysis of an ERG24 homolog from Schizosaccharomyces pombe." Smith S. Gene 155:139-140(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: SP66.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L36039 mRNA. Translation: AAA74121.1. CU329671 Genomic DNA. Translation: CAA19037.1.
PIR	JC4057.
RefSeq	NP_596767.1. NM_001023787.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPBC16G5.18-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC16G5.18.1; SPBC16G5.18.1:pep; SPBC16G5.18.
GeneID	2540113.
KEGG	spo:SPBC16G5.18.
Organism-specific databases
PomBase	SPBC16G5.18.
Phylogenomic databases
eggNOG	NOG72042.
HOGENOM	HOG000193296.
KO	K00222.
OMA	RPGMLLW.
OrthoDB	EOG4R273N.
Enzyme and pathway databases
UniPathway	UPA00770; UER00755.
Family and domain databases
InterPro	IPR001171. Ergosterol_biosynth_ERG4_ERG24. IPR018083. Sterol_reductase_CS. [Graphical view]
Pfam	PF01222. ERG4_ERG24. 1 hit. [Graphical view]
PROSITE	PS01017. STEROL_REDUCT_1. 1 hit. PS01018. STEROL_REDUCT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20801249.

Entry information

Entry name

ERG24_SCHPO

Accession

Primary (citable) accession number: Q09195

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	November 1, 1996
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program