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Protein

Delta(14)-sterol reductase

Gene

erg24

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Reduces the C14=C15 double bond of 4,4-dimethyl-cholesta-8,14,24-trienol to produce 4,4-dimethyl-cholesta-8,24-dienol.By similarity

Catalytic activityi

4,4-dimethyl-5-alpha-cholesta-8,24-dien-3-beta-ol + NADP+ = 4,4-dimethyl-5-alpha-cholesta-8,14,24-trien-3-beta-ol + NADPH.

Pathwayi

GO - Molecular functioni

  1. delta14-sterol reductase activity Source: PomBase

GO - Biological processi

  1. ergosterol biosynthetic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiREACT_272874. Cholesterol biosynthesis.
REACT_297664. Activation of gene expression by SREBF (SREBP).
UniPathwayiUPA00770; UER00755.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta(14)-sterol reductase (EC:1.3.1.70)
Alternative name(s):
C-14 sterol reductase
Sterol C14-reductase
Gene namesi
Name:erg24
ORF Names:SPBC16G5.18
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC16G5.18.
PomBaseiSPBC16G5.18.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei19 – 3921HelicalSequence AnalysisAdd
BLAST
Transmembranei112 – 13221HelicalSequence AnalysisAdd
BLAST
Transmembranei370 – 39021HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: PomBase
  2. endoplasmic reticulum membrane Source: PomBase
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 424424Delta(14)-sterol reductasePRO_0000207493Add
BLAST

Proteomic databases

MaxQBiQ09195.

Interactioni

Protein-protein interaction databases

MINTiMINT-4693879.
STRINGi4896.SPBC16G5.18-1.

Family & Domainsi

Sequence similaritiesi

Belongs to the ERG4/ERG24 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG72042.
HOGENOMiHOG000193296.
InParanoidiQ09195.
KOiK00222.
OMAiTELACKG.
OrthoDBiEOG7HB5MF.
PhylomeDBiQ09195.

Family and domain databases

InterProiIPR001171. Ergosterol_biosynth_ERG4_ERG24.
IPR018083. Sterol_reductase_CS.
[Graphical view]
PfamiPF01222. ERG4_ERG24. 1 hit.
[Graphical view]
PROSITEiPS01017. STEROL_REDUCT_1. 1 hit.
PS01018. STEROL_REDUCT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09195-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKGAVKKEK FEYEFFGPIG ALGVTVLTTV VSFGSFYICN EEGCPAKFSK
60 70 80 90 100
ISHIFKKTPL FDQKSLILYL LWFSTLTLLW KCTNGKWAKG TPIDDKGTRL
110 120 130 140 150
LYKINGFNSA CLILGVVCTS IYLLGASCME FIWDNFLQLM FAAYVFSVVL
160 170 180 190 200
CTFCYVQSFF GKQQLAKGGT SGNILFDWFI GRSLNPRIGN FDIKCFCELR
210 220 230 240 250
PGLILWVVFD IAFACHQYLV LGGRITDSMV LVIIFHTWYV LDSLINESAV
260 270 280 290 300
LTTMDITTDG FGYMLSFGDL VWVPFLYSLQ ARYLAFHPVD LGLVKTLAIL
310 320 330 340 350
CLQFLGYYIF RGANGQKNRF RSNPNDPKLK HLKFIQTKRG TKLLTSGWWG
360 370 380 390 400
MARHINYFGD WIMAWAWCLP AGFGSPIPYF YVAYFGVLLV HRNARDDHKC
410 420
RVKYGEDWEK YCKAVKYRII PYVY
Length:424
Mass (Da):48,560
Last modified:November 1, 1996 - v1
Checksum:i1D3CE704F1E5EF5B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36039 mRNA. Translation: AAA74121.1.
CU329671 Genomic DNA. Translation: CAA19037.1.
PIRiJC4057.
RefSeqiNP_596767.1. NM_001023787.2.

Genome annotation databases

EnsemblFungiiSPBC16G5.18.1; SPBC16G5.18.1:pep; SPBC16G5.18.
GeneIDi2540113.
KEGGispo:SPBC16G5.18.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36039 mRNA. Translation: AAA74121.1.
CU329671 Genomic DNA. Translation: CAA19037.1.
PIRiJC4057.
RefSeqiNP_596767.1. NM_001023787.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4693879.
STRINGi4896.SPBC16G5.18-1.

Proteomic databases

MaxQBiQ09195.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC16G5.18.1; SPBC16G5.18.1:pep; SPBC16G5.18.
GeneIDi2540113.
KEGGispo:SPBC16G5.18.

Organism-specific databases

EuPathDBiFungiDB:SPBC16G5.18.
PomBaseiSPBC16G5.18.

Phylogenomic databases

eggNOGiNOG72042.
HOGENOMiHOG000193296.
InParanoidiQ09195.
KOiK00222.
OMAiTELACKG.
OrthoDBiEOG7HB5MF.
PhylomeDBiQ09195.

Enzyme and pathway databases

UniPathwayiUPA00770; UER00755.
ReactomeiREACT_272874. Cholesterol biosynthesis.
REACT_297664. Activation of gene expression by SREBF (SREBP).

Miscellaneous databases

NextBioi20801249.
PROiQ09195.

Family and domain databases

InterProiIPR001171. Ergosterol_biosynth_ERG4_ERG24.
IPR018083. Sterol_reductase_CS.
[Graphical view]
PfamiPF01222. ERG4_ERG24. 1 hit.
[Graphical view]
PROSITEiPS01017. STEROL_REDUCT_1. 1 hit.
PS01018. STEROL_REDUCT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequence analysis of an ERG24 homolog from Schizosaccharomyces pombe."
    Smith S.
    Gene 155:139-140(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: SP66.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiERG24_SCHPO
AccessioniPrimary (citable) accession number: Q09195
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1996
Last modified: April 29, 2015
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.