Reviewed, UniProtKB/Swiss-Prot Q09178 (JAK1_CYPCA)

Names and origin

Protein names

Recommended name:
    Tyrosine-protein kinase Jak1
    EC=2.7.10.2
Alternative name(s):
    Janus kinase 1
      Short name=Jak-1

Gene names

Name:

jak1

Organism

Cyprinus carpio (Common carp)

Taxonomic identifier

7962 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Cyprinus

Protein attributes

Sequence length	1156 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Tyrosine kinase of the non-receptor type By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subcellular location	Endomembrane system; Peripheral membrane protein By similarity. Note: Wholly intracellular, possibly membrane associated By similarity.
Domain	Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain. Contains 1 SH2 domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat SH2 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoskeleton Inferred from electronic annotation. Source: InterPro extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW Janus kinase activity Inferred from electronic annotation. Source: InterPro non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1156	1156	Tyrosine-protein kinase Jak1		PRO_0000088111
Regions
	Domain	32 – 419	388	FERM
	Domain	438 – 543	106	SH2; atypical
	Domain	583 – 857	275	Protein kinase 1
	Domain	875 – 1153	279	Protein kinase 2
	Nucleotide binding	881 – 889	9	ATP By similarity
	Compositional bias	337 – 359	23	Lys-rich (basic)
Sites
	Active site	1003	1	Proton acceptor By similarity
	Binding site	908	1	ATP By similarity
Amino acid modifications
	Modified residue	1034	1	Phosphotyrosine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q09178-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: FF54BDDD2F9666F5	FASTA	1,156	132,246
	10 20 30 40 50 60 MPELAVMELG RQLCGKMKKQ RKAEMTVLTV MKGLEIHFYL PDTHQLEYFK DCHTAEDLCV 70 80 90 100 110 120 EGCQEDATSH LCADNLFALS EESQDLWYAP NHAFKITEET SIKLHYRMRF YFTNWHAPVR 130 140 150 160 170 180 TESPVWRHSL FKHKGVSVSP KGPEGTPLLD AASLEYLFAQ GQYDFLRGLA PVRAPQNEAE 190 200 210 220 230 240 KHEIENECLG MAVLAITHHA KSNDLPLSGV GAETSYKRFI PDSLNRTIKQ RNFSHVYVYN 250 260 270 280 290 300 NVFKNFLNEF NSKTIQDSNI TLYDLKVKYL STLETLTQGL GRETIEPKIL KVSGESDGSP 310 320 330 340 350 360 ALTLPSGDDG LGYEVQVSGT TGISWRRKPV PNILIVKDKT KSKKNKADKQ SKKEMTKRKT 370 380 390 400 410 420 VMTIFSDFFE ITHIVIKESC ATIYSQDNKT MELDLFYRDA ALSFAALVDG YFRLTVDAHH 430 440 450 460 470 480 YLCTEVAPSS VVQNLENGCH GPICTEYAIH KLRQEGNEEG TYVLRWSCTD YNYIIMTVVC 490 500 510 520 530 540 IEMDLCESRP VPQYKNFQIE TSPQGYRLYG TDTFRPTLKE LLEHLQGQNL RTENLRFQPV 550 560 570 580 590 600 LVGLGQPRKI SNLLVMTRDR EPDSQRQPQV SQLSFHRILK EEIVQGEHLG RGTRTNIYSG 610 620 630 640 650 660 VLKLKSEDDD DMGGYSQEVK VILKVLGSGH RDISLAFFET ASMMRQISHK HIALLYGVCV 670 680 690 700 710 720 RHQENIMVEE FVQYGPLDLF MRRQSIPLST AWKFQVAKQL AGALSYLEDK KLVHGNVCSK 730 740 750 760 770 780 NILVARDGLD GEGGPFIKLS DPGIPITVLS REECVDRLPW IAPECVQDTA NLSIAADKWG 790 800 810 820 830 840 FGTTLWEICY NGEIPLKDKK LTEKERFYAA QCQLASPDCE ELAKLMTHCM TYDPRQRLFF 850 860 870 880 890 900 RAIVRDIDMV EKQNPSIQPV PMLEVDPTVF EKRFLKKIRD LGEGHFGKVE LCRYDPRGDR 910 920 930 940 950 960 TGELVAVKSL KPENREEQSS NLWREIHILR ELYHENIVKY KGIWHEEGGR SIKLIMEFLP 970 980 990 1000 1010 1020 AGSLKEYLPR NKAHIDLKTL LNYAVQICQG MDLLASRNYI HRDLAARNVL VENENTVKIG 1030 1040 1050 1060 1070 1080 DFGLTKSIKD NEGYYTVKDD LDSPVFWYAP ECLIHCKFYR ASDVWSFGVT MYELLTYCDI 1090 1100 1110 1120 1130 1140 SCSPMSVFLT MIGPTHGQMT VTRLVKVLEE GKRLPKPDGC SDRLYCLMRR CWEATPEKRI 1150 DFKGLIANFQ QMIDNQ

References

[1]	"Expression, characterization, and genomic structure of carp JAK1 kinase gene." Chang M.S., Chang G.D., Leu J.H., Huang F.L., Chou C.K., Huang C.J., Lo T.B. DNA Cell Biol. 15:827-844(1996) [PubMed: 8892755] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L24895 mRNA. Translation: AAB38157.1. U53694 , U53686, U53687, U53688, U53689, U53690, U53691, U53692, U53693 Genomic DNA. Translation: AAB38256.1.
3D structure databases
HSSP	HSSP built from PDB template 1FGK based on UniProtKB P11362.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q09178.
Enzyme and pathway databases
BRENDA	2.7.10.2. 3298.
Family and domain databases
InterPro	IPR019749. Band_41_domain. IPR019747. FERM_CS. IPR000299. FERM_domain. IPR009127. JAK. IPR009128. JAK1. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR000980. SH2. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. IPR016251. TyrPK_Jak. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 2 hits. [Graphical view]
PIRSF	PIRSF000636. TyrPK_Jak. 1 hit.
PRINTS	PR01823. JANUSKINASE. PR01824. JANUSKINASE1. PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 2 hits. PD000093. SH2. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00295. B41. 1 hit. SM00252. SH2. 1 hit. SM00219. TyrKc. 2 hits. [Graphical view]
PROSITE	PS00660. FERM_1. False negative. PS00661. FERM_2. False negative. PS50057. FERM_3. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 2 hits. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

JAK1_CYPCA

Accession

Primary (citable) accession number: Q09178

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	October 13, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)