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Protein

Splicing factor U2AF 23 kDa subunit

Gene

SPAP8A3.06

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for the splicing of pre-mRNA. The SF1-U2AF59-U2AF23 complex has a role in the recognition of the branch site (5'-UACUAAC-3'), the pyrimidine tract and the 3'-splice site at the 3'-end of introns.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri12 – 40C3H1-type 1PROSITE-ProRule annotationAdd BLAST29
Zinc fingeri143 – 170C3H1-type 2PROSITE-ProRule annotationAdd BLAST28

GO - Molecular functioni

GO - Biological processi

  • mRNA cis splicing, via spliceosome Source: PomBase
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Splicing factor U2AF 23 kDa subunit
Alternative name(s):
U2 auxiliary factor 23 kDa subunit
Short name:
U2AF23
U2 snRNP auxiliary factor small subunit
Gene namesi
ORF Names:SPAP8A3.06
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAP8A3.06.
PomBaseiSPAP8A3.06.

Subcellular locationi

GO - Cellular componenti

  • commitment complex Source: PomBase
  • cytosol Source: PomBase
  • nucleus Source: PomBase
  • U2AF Source: PomBase
  • U2-type prespliceosome Source: PomBase
  • U2-type spliceosomal complex Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000819981 – 216Splicing factor U2AF 23 kDa subunitAdd BLAST216

Proteomic databases

MaxQBiQ09176.
PRIDEiQ09176.

Interactioni

Subunit structurei

Forms a heterodimer with the U2AF large subunit. Can also form a homodimer. U2AF large subunit (U2AF59), U2AF small subunit (U2AF23) and SF1 (bpb1) interact to form a complex required for complex A formation. Interacts with cwf13.2 Publications

Protein-protein interaction databases

BioGridi278942. 10 interactors.
IntActiQ09176. 1 interactor.
MINTiMINT-4693778.

Structurei

Secondary structure

1216
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi19 – 24Combined sources6
Helixi30 – 32Combined sources3
Beta strandi34 – 36Combined sources3
Beta strandi44 – 48Combined sources5
Helixi55 – 57Combined sources3
Helixi61 – 63Combined sources3
Helixi66 – 84Combined sources19
Helixi85 – 87Combined sources3
Beta strandi90 – 96Combined sources7
Turni101 – 105Combined sources5
Beta strandi107 – 113Combined sources7
Helixi114 – 124Combined sources11
Beta strandi137 – 139Combined sources3
Helixi144 – 147Combined sources4
Helixi150 – 154Combined sources5
Helixi160 – 162Combined sources3
Helixi173 – 191Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4YH8X-ray1.70A1-216[»]
ProteinModelPortaliQ09176.
SMRiQ09176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 141RRMPROSITE-ProRule annotationAdd BLAST98

Sequence similaritiesi

Contains 2 C3H1-type zinc fingers.PROSITE-ProRule annotation
Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri12 – 40C3H1-type 1PROSITE-ProRule annotationAdd BLAST29
Zinc fingeri143 – 170C3H1-type 2PROSITE-ProRule annotationAdd BLAST28

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOGENOMiHOG000178619.
InParanoidiQ09176.
KOiK12836.
OMAiHETSECQ.
OrthoDBiEOG092C30A1.
PhylomeDBiQ09176.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
IPR009145. U2AF_small.
IPR000571. Znf_CCCH.
[Graphical view]
PANTHERiPTHR12620. PTHR12620. 1 hit.
PfamiPF00076. RRM_1. 1 hit.
PF00642. zf-CCCH. 2 hits.
[Graphical view]
PRINTSiPR01848. U2AUXFACTOR.
SMARTiSM00361. RRM_1. 1 hit.
SM00356. ZnF_C3H1. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
PS50103. ZF_C3H1. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09176-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASHLASIYG TEQDKVNCSF YYKIGACRHG ERCSRKHVKP NFSQTILCPN
60 70 80 90 100
MYKNPIHEPN GKKFTQRELA EQFDAFYEDM FCEFSKYGEV EQLVVCDNVG
110 120 130 140 150
DHLVGNVYVR FKYEESAQNA IDDLNSRWYS QRPVYAELSP VTDFREACCR
160 170 180 190 200
QHETSECQRG GLCNFMHAKK PSPQLLRDLV LAQRKYLALN AAEEMKKEPN
210
SDSTNRWVSV TAERKN
Length:216
Mass (Da):25,030
Last modified:December 15, 1998 - v2
Checksum:iAAEDD3DE2DF3507D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48234 Genomic DNA. Translation: AAC49805.1.
CU329670 Genomic DNA. Translation: CAB55173.1.
PIRiT39243.
RefSeqiNP_594945.1. NM_001020376.2.

Genome annotation databases

EnsemblFungiiSPAP8A3.06.1; SPAP8A3.06.1:pep; SPAP8A3.06.
GeneIDi2542482.
KEGGispo:SPAP8A3.06.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48234 Genomic DNA. Translation: AAC49805.1.
CU329670 Genomic DNA. Translation: CAB55173.1.
PIRiT39243.
RefSeqiNP_594945.1. NM_001020376.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4YH8X-ray1.70A1-216[»]
ProteinModelPortaliQ09176.
SMRiQ09176.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278942. 10 interactors.
IntActiQ09176. 1 interactor.
MINTiMINT-4693778.

Proteomic databases

MaxQBiQ09176.
PRIDEiQ09176.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAP8A3.06.1; SPAP8A3.06.1:pep; SPAP8A3.06.
GeneIDi2542482.
KEGGispo:SPAP8A3.06.

Organism-specific databases

EuPathDBiFungiDB:SPAP8A3.06.
PomBaseiSPAP8A3.06.

Phylogenomic databases

HOGENOMiHOG000178619.
InParanoidiQ09176.
KOiK12836.
OMAiHETSECQ.
OrthoDBiEOG092C30A1.
PhylomeDBiQ09176.

Miscellaneous databases

PROiQ09176.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
IPR009145. U2AF_small.
IPR000571. Znf_CCCH.
[Graphical view]
PANTHERiPTHR12620. PTHR12620. 1 hit.
PfamiPF00076. RRM_1. 1 hit.
PF00642. zf-CCCH. 2 hits.
[Graphical view]
PRINTSiPR01848. U2AUXFACTOR.
SMARTiSM00361. RRM_1. 1 hit.
SM00356. ZnF_C3H1. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
PS50103. ZF_C3H1. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiU2AF1_SCHPO
AccessioniPrimary (citable) accession number: Q09176
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: November 2, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.