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Protein

Dibasic-processing endoprotease

Gene

krp1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Membrane-bound, subtilisin-like serine protease that processes the P-factor precursor and other precursor proteins. Essential for cell viability. Cleaves substrate on the C-terminal side of dibasic residues.1 Publication

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei162Charge relay systemBy similarity1
Active sitei200Charge relay systemBy similarity1
Active sitei371Charge relay systemBy similarity1

GO - Molecular functioni

  • endopeptidase activity Source: PomBase
  • peptidase activity, acting on L-amino acid peptides Source: PomBase
  • serine-type endopeptidase activity Source: PomBase
  • serine-type endopeptidase inhibitor activity Source: PomBase

GO - Biological processi

  • peptide mating pheromone maturation involved in posititve regulation of conjugation with cellular fusion Source: PomBase
  • protein autoprocessing Source: PomBase
  • proteolysis Source: PomBase

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
LigandCalcium

Protein family/group databases

MEROPSiS08.A55

Names & Taxonomyi

Protein namesi
Recommended name:
Dibasic-processing endoprotease (EC:3.4.21.-)
Alternative name(s):
KEX2-related protease
Gene namesi
Name:krp1
Synonyms:krp
ORF Names:SPAC22E12.09c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC22E12.09c
PomBaseiSPAC22E12.09c krp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini103 – 668LumenalSequence analysisAdd BLAST566
Transmembranei669 – 693HelicalSequence analysisAdd BLAST25
Topological domaini694 – 709CytoplasmicSequence analysisAdd BLAST16

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi425F → S in JY965; temperature-sensitive. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
PropeptideiPRO_000002704623 – 82Sequence analysisAdd BLAST60
PropeptideiPRO_000002704783 – 102Sequence analysisAdd BLAST20
ChainiPRO_0000027048103 – 709Dibasic-processing endoproteaseAdd BLAST607

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi155N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi216 ↔ 363By similarity
Disulfide bondi308 ↔ 338By similarity
Glycosylationi463N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi471N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi620N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiQ09175
PaxDbiQ09175
PRIDEiQ09175

PTM databases

iPTMnetiQ09175

Miscellaneous databases

PMAP-CutDBQ09175

Interactioni

Protein-protein interaction databases

BioGridi279032, 5 interactors
STRINGi4896.SPAC22E12.09c.1

Structurei

3D structure databases

ProteinModelPortaliQ09175
SMRiQ09175
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini157 – 440Peptidase S8Add BLAST284
Domaini449 – 588P/Homo BPROSITE-ProRule annotationAdd BLAST140

Sequence similaritiesi

Belongs to the peptidase S8 family. Furin subfamily.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000192536
InParanoidiQ09175
KOiK01341
OMAiPQRGVLK
OrthoDBiEOG092C0JIG
PhylomeDBiQ09175

Family and domain databases

CDDicd04059 Peptidases_S8_Protein_converta, 1 hit
Gene3Di2.60.120.260, 1 hit
3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR008979 Galactose-bd-like_sf
IPR034182 Kexin/furin
IPR002884 P_dom
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR023827 Peptidase_S8_Asp-AS
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
PfamiView protein in Pfam
PF01483 P_proprotein, 1 hit
PF00082 Peptidase_S8, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF49785 SSF49785, 1 hit
SSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS51829 P_HOMO_B, 1 hit
PS00136 SUBTILASE_ASP, 1 hit
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q09175-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPALLCGPI LAIFLQFLVS SCSPLENDDL FLVQVEPEVD PVVAAEAIGA
60 70 80 90 100
KYVRPLLNLK YHHLIKLHKG SDDSVQSSIR KRGIDAGILE LERQTPRWRY
110 120 130 140 150
KRDASESDEL LNEFSNHFGI SDPLFYGQWH IFNSNNPGHD LNLREVWDAG
160 170 180 190 200
YFGENVTVAF VDDGIDFKHP DLQAAYTSLG SWDFNDNIAD PLPKLSDDQH
210 220 230 240 250
GTRCAGEVAA AWNDVCGVGI APRAKVAGLR ILSAPITDAV ESEALNYGFQ
260 270 280 290 300
TNHIYSCSWG PADDGRAMDA PNTATRRALM NGVLNGRNGL GSIFVFASGN
310 320 330 340 350
GGHYHDNCNF DGYTNSIFSA TIGAVDAEHK IPFYSEVCAA QLVSAYSSGS
360 370 380 390 400
HLSILTTNPE GTCTRSHGGT SAAAPLASAV YALALSIRPD LSWRDIQHIT
410 420 430 440 450
VYSASPFDSP SQNAEWQKTP AGFQFSHHFG FGKLDASKFV EVAKDWQVVN
460 470 480 490 500
PQTWLIAPEI NVNKSFGSVN NETITEMVSE FTVTKDMIEK SNFKRLEHVT
510 520 530 540 550
VRVCIPFNRR GALEILLESP SGIRSILASE RPYDENSKGF LDWTFMTVQH
560 570 580 590 600
WAEPPEGVWK LLVNDRSGGK HEGTFENWQL ALWGESENPS NTAPLPYDTL
610 620 630 640 650
ELPKEMVLGI YSEPNSDLTN SSTLLSPTST SFTSYTVSAT ATPTSTSHIP
660 670 680 690 700
IPTVLPPTQP VLEPSYREIV AFITFFLLFA FIFVAVIWTW ISAFWKAKAP

PPLSQQEIA
Length:709
Mass (Da):78,127
Last modified:November 1, 1995 - v1
Checksum:i414FE9B89CBE0840
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82435 Genomic DNA Translation: CAA57818.1
CU329670 Genomic DNA Translation: CAA93896.1
PIRiS51793
RefSeqiNP_594835.1, NM_001020264.2

Genome annotation databases

EnsemblFungiiSPAC22E12.09c.1; SPAC22E12.09c.1:pep; SPAC22E12.09c
GeneIDi2542576
KEGGispo:SPAC22E12.09c

Similar proteinsi

Entry informationi

Entry nameiKRP1_SCHPO
AccessioniPrimary (citable) accession number: Q09175
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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