Q09174 (GMA12_SCHPO) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 29, 2013.
Version 98.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Alpha-1,2-galactosyltransferase EC=2.4.1.- | ||||
| Gene names |
| ||||
| Organism | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome] | ||||
| Taxonomic identifier | 284812 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › ![]() |
Protein attributes
| Sequence length | 375 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Involved in the O- and N-linked oligosaccharide modification of proteins transported through the Golgi stack. This occurs in cis Golgi where the enzyme transfers galactose from UDP-galactose to a variety of mannose based acceptors. Ref.1 Ref.3 |
| Subcellular location | Golgi apparatus membrane; Single-pass type II membrane protein Ref.1. |
| Post-translational modification | O-glycosylated. |
| Sequence similarities | Belongs to the glycosyltransferase 34 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Golgi apparatus Membrane |
| Domain | Signal-anchor Transmembrane Transmembrane helix |
| Molecular function | Glycosyltransferase Transferase |
| PTM | Glycoprotein |
| Technical term | Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | fungal-type cell wall biogenesis Inferred from mutant phenotype Ref.3. Source: PomBase protein N-linked glycosylationInferred from mutant phenotype Ref.3. Source: PomBase protein O-linked glycosylationInferred from mutant phenotype Ref.3. Source: PomBase protein galactosylationInferred from mutant phenotype Ref.3. Source: PomBase |
| Cellular_component | Golgi membrane Inferred from direct assay Ref.1. Source: PomBase integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular_function | alpha-1,2-galactosyltransferase activity Inferred from mutant phenotype Ref.1. Source: PomBase |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 375 | 375 | Alpha-1,2-galactosyltransferase | PRO_0000215163 | |||||
Regions | |||||||||
| Topological domain | 1 – 2 | 2 | Cytoplasmic Potential | ||||||
| Transmembrane | 3 – 23 | 21 | Helical; Signal-anchor for type II membrane protein; Potential | ||||||
| Topological domain | 24 – 375 | 352 | Lumenal Potential | ||||||
| Compositional bias | 43 – 95 | 53 | Ser/Thr/Val-rich | ||||||
Experimental info | |||||||||
| Sequence conflict | 161 | 1 | W → R AA sequence Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Localization of an alpha 1,2 galactosyltransferase activity to the Golgi apparatus of Schizosaccharomyces pombe." Chappell T.G., Hajibagheri M.A.N., Ayscough K., Pierce M., Warren G. Mol. Biol. Cell 5:519-528(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 35-42; 160-164; 174-178 AND 361-373, FUNCTION, SUBCELLULAR LOCATION. |
| [2] | "The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. Nurse P.Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843. |
| [3] | "Differences in in vivo acceptor specificity of two galactosyltransferases, the gmh3+ and gma12+ gene products from Schizosaccharomyces pombe." Yoko-o T., Roy S.K., Jigami Y. Eur. J. Biochem. 257:630-637(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Z30917 Genomic DNA. Translation: CAA83200.1. CU329672 Genomic DNA. Translation: CAA19268.1. |
| PIR | T41561. |
| RefSeq | NP_587775.1. NM_001022768.2. |
3D structure databases | |
| ProteinModelPortal | Q09174. |
| ModBase | Search... |
Protein-protein interaction databases | |
| MINT | MINT-4693747. |
| STRING | 4896.SPCC736.04c-1. |
Protein family/group databases | |
| CAZy | GT34. Glycosyltransferase Family 34. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | SPCC736.04c.1; SPCC736.04c.1:pep; SPCC736.04c. |
| GeneID | 2539101. |
| KEGG | spo:SPCC736.04c. |
Organism-specific databases | |
| PomBase | SPCC736.04c. |
Phylogenomic databases | |
| eggNOG | NOG293649. |
| HOGENOM | HOG000112744. |
| KO | K10971. |
| OMA | RIDEHRR. |
| OrthoDB | EOG49PF7F. |
Family and domain databases | |
| InterPro | IPR008630. Glyco_trans_34. [Graphical view] |
| Pfam | PF05637. Glyco_transf_34. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 20800273. |
Entry information
| Entry name | GMA12_SCHPO | ||||||||
| Accession | Primary (citable) accession number: Q09174 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Schizosaccharomyces pombe Schizosaccharomyces pombe: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with
