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Reviewed, UniProtKB/Swiss-Prot Q09174 (GMA12_SCHPO)

Last modified June 16, 2009. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Alpha-1,2-galactosyltransferase
    EC=2.4.1.-
Gene names
Name: gma12
ORF Names: SPCC736.04c
OrganismSchizosaccharomyces pombe (Fission yeast) [Complete proteome]
Taxonomic identifier4896 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length375 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Involved in the O- and N-linked oligosaccharide modification of proteins transported through the Golgi stack. This occurs in cis Golgi where the enzyme transfers galactose from UDP-galactose to a variety of mannose based acceptors. Ref.1 Ref.3

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein. Ref.1

Post-translational modification

O-glycosylated.

Sequence similarities

Belongs to the glycosyltransferase 34 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 375375Alpha-1,2-galactosyltransferase
PRO_0000215163

Regions

Topological domain1 – 22Cytoplasmic Potential
Transmembrane3 – 2321Signal-anchor for type II membrane protein Potential
Topological domain24 – 375352Lumenal Potential
Compositional bias43 – 9553Ser/Thr/Val-rich

Experimental info

Sequence conflict1611W → R AA sequence Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q09174-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 05ADA38C352B3B3C

FASTA37542,621
        10         20         30         40         50         60 
MRFAPYLISA VVITTIILGG AWWTSAMDTK LQTKMKEIID QHTSTWTPVV SSVTSTQTDT 

        70         80         90        100        110        120 
LRVTISEVVS VTATLTETFT ATPTVTSVVH ALATTDPHPD NSKIVILMGS NFQNDANSPL 

       130        140        150        160        170        180 
HPFAQSIIKN RREYAERHGY KFEFLDADAY ASRVTGHLMP WVKVPMLQDT MKKYPDAEWI 

       190        200        210        220        230        240 
WWLDHDALVM NKDLNVVDHV LKHDRLNTIL TREAEYKSGA GIPADGFRTP KDQDAKDVHF 

       250        260        270        280        290        300 
IISQDFNGIN AGSLFIRNSE VGRWIVDLWF EPLYLDHIQG YAEQQAFSHM VFYHPQVYKH 

       310        320        330        340        350        360 
VGVVPLKAIN AYDFDDNIWG YDDGDLCIHF AGCNYFKNCP EKFLKYAQIL SSKQGSDWMS 

       370 
AQEKDHIQNL LKPSS 

« Hide

References

« Hide 'large scale' references
[1]"Localization of an alpha 1,2 galactosyltransferase activity to the Golgi apparatus of Schizosaccharomyces pombe."
Chappell T.G., Hajibagheri M.A.N., Ayscough K., Pierce M., Warren G.
Mol. Biol. Cell 5:519-528(1994) [PubMed: 7522655] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 35-42; 160-164; 174-178 AND 361-373, FUNCTION, SUBCELLULAR LOCATION.
[2]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed: 11859360] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 38366 / 972.
[3]"Differences in in vivo acceptor specificity of two galactosyltransferases, the gmh3+ and gma12+ gene products from Schizosaccharomyces pombe."
Yoko-o T., Roy S.K., Jigami Y.
Eur. J. Biochem. 257:630-637(1998) [PubMed: 9839953] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

Z30917 Genomic DNA. Translation: CAA83200.1.
CU329672 Genomic DNA. Translation: CAA19268.1.
PIRT41561.
RefSeqNP_587775.1.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGT34. Glycosyltransferase Family 34.

Genome annotation databases

GeneID2539101.
KEGGspo:SPCC736.04c.
NMPDRfig|4896.1.peg.113.

Organism-specific databases

GeneDB_SpombeSPCC736.04c.

Phylogenomic databases

OMAQ09174. RIDEHRR.

Enzyme and pathway databases

BioCycSPOM-XXX-01:SPOM-XXX-01-002132-MON.

Gene expression databases

ArrayExpressQ09174.

Family and domain databases

InterProIPR008630. Glyco_trans_34.
[Graphical view]
PfamPF05637. Glyco_transf_34. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGMA12_SCHPO
AccessionPrimary (citable) accession number: Q09174
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents