Reviewed, UniProtKB/Swiss-Prot Q09174 (GMA12_SCHPO)

Names and origin

Protein names

Recommended name:
    Alpha-1,2-galactosyltransferase
    EC=2.4.1.-

Gene names

Name:	gma12
ORF Names:	SPCC736.04c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the O- and N-linked oligosaccharide modification of proteins transported through the Golgi stack. This occurs in cis Golgi where the enzyme transfers galactose from UDP-galactose to a variety of mannose based acceptors. Ref.1 Ref.3
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein. Ref.1
Post-translational modification	O-glycosylated.
Sequence similarities	Belongs to the glycosyltransferase 34 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	fungal-type cell wall biogenesis Ref.3 Inferred from mutant phenotype. Source: GeneDB_SPombe protein amino acid N-linked glycosylation Ref.3 Inferred from mutant phenotype. Source: GeneDB_SPombe protein amino acid O-linked glycosylation Ref.3 Inferred from mutant phenotype. Source: GeneDB_SPombe protein amino acid galactosylation Ref.3 Inferred from mutant phenotype. Source: GeneDB_SPombe
Cellular component	Golgi membrane Inferred from direct assay. Source: GeneDB_SPombe integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,2-galactosyltransferase activity Inferred from mutant phenotype. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Alpha-1,2-galactosyltransferase		PRO_0000215163
Regions
	Topological domain	1 – 2	2	Cytoplasmic Potential
	Transmembrane	3 – 23	21	Signal-anchor for type II membrane protein Potential
	Topological domain	24 – 375	352	Lumenal Potential
	Compositional bias	43 – 95	53	Ser/Thr/Val-rich
Experimental info
	Sequence conflict	161	1	W → R AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q09174-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 05ADA38C352B3B3C	FASTA	375	42,621
	10 20 30 40 50 60 MRFAPYLISA VVITTIILGG AWWTSAMDTK LQTKMKEIID QHTSTWTPVV SSVTSTQTDT 70 80 90 100 110 120 LRVTISEVVS VTATLTETFT ATPTVTSVVH ALATTDPHPD NSKIVILMGS NFQNDANSPL 130 140 150 160 170 180 HPFAQSIIKN RREYAERHGY KFEFLDADAY ASRVTGHLMP WVKVPMLQDT MKKYPDAEWI 190 200 210 220 230 240 WWLDHDALVM NKDLNVVDHV LKHDRLNTIL TREAEYKSGA GIPADGFRTP KDQDAKDVHF 250 260 270 280 290 300 IISQDFNGIN AGSLFIRNSE VGRWIVDLWF EPLYLDHIQG YAEQQAFSHM VFYHPQVYKH 310 320 330 340 350 360 VGVVPLKAIN AYDFDDNIWG YDDGDLCIHF AGCNYFKNCP EKFLKYAQIL SSKQGSDWMS 370 AQEKDHIQNL LKPSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Localization of an alpha 1,2 galactosyltransferase activity to the Golgi apparatus of Schizosaccharomyces pombe." Chappell T.G., Hajibagheri M.A.N., Ayscough K., Pierce M., Warren G. Mol. Biol. Cell 5:519-528(1994) [PubMed: 7522655] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 35-42; 160-164; 174-178 AND 361-373, FUNCTION, SUBCELLULAR LOCATION.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[3]	"Differences in in vivo acceptor specificity of two galactosyltransferases, the gmh3+ and gma12+ gene products from Schizosaccharomyces pombe." Yoko-o T., Roy S.K., Jigami Y. Eur. J. Biochem. 257:630-637(1998) [PubMed: 9839953] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z30917 Genomic DNA. Translation: CAA83200.1. CU329672 Genomic DNA. Translation: CAA19268.1.
PIR	T41561.
RefSeq	NP_587775.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT34. Glycosyltransferase Family 34.
Genome annotation databases
GeneID	2539101.
KEGG	spo:SPCC736.04c.
NMPDR	fig|4896.1.peg.113.
Organism-specific databases
GeneDB_Spombe	SPCC736.04c.
Phylogenomic databases
OMA	Q09174. RIDEHRR.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-002132-MON.
Gene expression databases
ArrayExpress	Q09174.
Family and domain databases
InterPro	IPR008630. Glyco_trans_34. [Graphical view]
Pfam	PF05637. Glyco_transf_34. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GMA12_SCHPO

Accession

Primary (citable) accession number: Q09174

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)