Q09173 (PP2C3_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 2C homolog 3
Short name=PP2C-3
EC=3.1.3.16

Gene names

Name:	ptc3
ORF Names:	SPAC2G11.07c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Has an important role in osmotic stability and cell shape control. It may negatively regulate the osmosensing signal transmitted through wis1 map kinase.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subunit structure	Monomer.
Sequence similarities	Belongs to the PP2C family.

Ontologies

Keywords
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	inactivation of MAPK activity involved in osmosensory signaling pathway Inferred from mutant phenotype Ref.1. Source: PomBase protein dephosphorylation Inferred from direct assay PubMed 8196617. Source: PomBase
Cellular_component	cytosol Inferred from direct assay PubMed 16823372. Source: PomBase nucleus Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from direct assay PubMed 8196617. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 414	414	Protein phosphatase 2C homolog 3		PRO_0000057772
Sites
	Metal binding	62	1	Manganese 1 By similarity
	Metal binding	62	1	Manganese 2 By similarity
	Metal binding	63	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	230	1	Manganese 2 By similarity
	Metal binding	279	1	Manganese 2 By similarity
Experimental info
	Sequence conflict	196	1	K → T in AAA67321. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q09173 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: EFF3A416625A2B11	FASTA	414	44,857
	10 20 30 40 50 60 MGQTLSEPVT EKHSVNGSNE FVLYGLSSMQ GWRISMEDAH SAILSMECSA VKDPVDFFAV 70 80 90 100 110 120 YDGHGGDKVA KWCGSNLPQI LEKNPDFQKG DFVNALKSSF LNADKAILDD DQFHTDPSGC 130 140 150 160 170 180 TATVVLRVGN KLYCANAGDS RTVLGSKGIA KPLSADHKPS NEAEKARICA AGGFVDFGRV 190 200 210 220 230 240 NGNLALSRAI GDFEFKNSNL EPEKQIVTAL PDVVVHEITD DDEFVVLACD GIWDCKTSQQ 250 260 270 280 290 300 VIEFVRRGIV AGTSLEKIAE NLMDNCIASD TETTGLGCDN MTVCIVALLQ ENDKSAWYKK 310 320 330 340 350 360 IADRVAANDG PCAPPEYAEN HGPGWRSGDN NKKVIVPPNF HQVKLNGSDG YDKDANENSK 370 380 390 400 410 EDDSTNGSLA AGFRWKEHFF PHKAEEENSS SETDIVNSNK DVADDHKEAV SAAD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Counteractive roles of protein phosphatase 2C (PP2C) and a MAP kinase kinase homolog in the osmoregulation of fission yeast." Shiozaki K., Russell P. EMBO J. 14:492-502(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L34882 Genomic DNA. Translation: AAA67321.1. CU329670 Genomic DNA. Translation: CAA91172.1.
PIR	S62462. T38573.
RefSeq	NP_593087.1. NM_001018485.2.
3D structure databases
ProteinModelPortal	Q09173.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPAC2G11.07c-1.
Proteomic databases
PaxDb	Q09173.
PRIDE	Q09173.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPAC2G11.07c.1; SPAC2G11.07c.1:pep; SPAC2G11.07c.
GeneID	2542008.
KEGG	spo:SPAC2G11.07c.
Organism-specific databases
PomBase	SPAC2G11.07c.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000233896.
KO	K14803.
OMA	HAGRING.
OrthoDB	EOG4RFQ28.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20803088.

Entry information

Entry name

PP2C3_SCHPO

Accession

Primary (citable) accession number: Q09173

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program