Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein kinase cds1

Gene

cds1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a role in the DNA replication-monitoring S/G2 checkpoint system. It is responsible for blocking mitosis in the S phase. It monitors DNA synthesis by interacting with DNA polymerase alpha and sends a signal to block the onset of mitosis while DNA synthesis is in progress. Phosphorylates rad60 and dna2.4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei196ATPPROSITE-ProRule annotation1
Active sitei294Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi173 – 181ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: PomBase
  • protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  • intra-S DNA damage checkpoint Source: UniProtKB
  • meiotic G2/MI DNA replication checkpoint Source: PomBase
  • mitotic DNA replication checkpoint Source: PomBase
  • negative regulation of protein export from nucleus Source: PomBase
  • peptidyl-serine phosphorylation Source: GO_Central
  • peptidyl-threonine phosphorylation Source: GO_Central
  • regulation of apoptotic process Source: GO_Central
  • regulation of mitotic DNA replication initiation Source: PomBase
  • signal transduction Source: PomBase
  • signal transduction in response to DNA damage Source: GO_Central

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1 5613
ReactomeiR-SPO-5693565 Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks
R-SPO-69473 G2/M DNA damage checkpoint
R-SPO-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-SPO-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase cds1 (EC:2.7.11.1)
Alternative name(s):
Checkpoint kinase cds1
Gene namesi
Name:cds1
ORF Names:SPCC18B5.11c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:SPCC18B5.11c
PomBaseiSPCC18B5.11c cds1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000858461 – 460Serine/threonine-protein kinase cds1Add BLAST460

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei443Phosphothreonine1 Publication1

Post-translational modificationi

Autophosphorylated.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ09170
PaxDbiQ09170
PRIDEiQ09170

PTM databases

iPTMnetiQ09170

Interactioni

Subunit structurei

Interacts with rad26.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
nrm1O429132EBI-3650524,EBI-15720278

Protein-protein interaction databases

BioGridi275533106 interactors.
DIPiDIP-46174N
IntActiQ09170 2 interactors.
STRINGi4896.SPCC18B5.11c.1

Structurei

3D structure databases

ProteinModelPortaliQ09170
SMRiQ09170
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 116FHAPROSITE-ProRule annotationAdd BLAST57
Domaini167 – 433Protein kinasePROSITE-ProRule annotationAdd BLAST267

Sequence similaritiesi

Phylogenomic databases

InParanoidiQ09170
KOiK02831
OMAiYKMLMEN
OrthoDBiEOG092C2FDZ
PhylomeDBiQ09170

Family and domain databases

CDDicd00060 FHA, 1 hit
InterProiView protein in InterPro
IPR000253 FHA_dom
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
IPR008984 SMAD_FHA_dom_sf
PfamiView protein in Pfam
PF00498 FHA, 1 hit
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00240 FHA, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF49879 SSF49879, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50006 FHA_DOMAIN, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Q09170-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEPEEATQA TQEAPLHVSQ NIAKQVVNNE NVFMKLVMTR MLDGKTEVIP
60 70 80 90 100
LTTDVHNGFW RFGRHKSCEV VLNGPRVSNF HFEIYQGHRN DSDESENVVF
110 120 130 140 150
LHDHSSNGTF LNFERLAKNS RTILSNGDEI RIGLGVPKDE ISFLCQVPVK
160 170 180 190 200
HSRDSQKNMI KSENSHYEII RTLGSGTFAV VKLAVEVNSG KWYAIKIINK
210 220 230 240 250
RKILLTSSEK RATEMFQREI DILKSLHHPG VVQCHEIFEN DDELFIVMEY
260 270 280 290 300
VEGGDLMDFL IANGSIDEQD CKPLLKQLLE TLLHLHKQGV THRDIKPENI
310 320 330 340 350
LITNDFHLKI SDFGLAKVIH GTGTFLETFC GTMGYLAPEV LKSKNVNLDG
360 370 380 390 400
GYDDKVDIWS LGCVLYVMLT ASIPFASSSQ AKCIELISKG AYPIEPLLEN
410 420 430 440 450
EISEEGIDLI NRMLEINPEK RISESEALQH PWFYTVSTHE HRTPPSSSEH
460
EATEQLNSSS
Length:460
Mass (Da):52,014
Last modified:January 11, 2001 - v2
Checksum:i4CEB963D3376DB54
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61R → G in CAA59410 (PubMed:7723827).Curated1
Sequence conflicti238F → C in CAA59410 (PubMed:7723827).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85040 mRNA Translation: CAA59410.1
AJ222869 Genomic DNA Translation: CAA11019.1
CU329672 Genomic DNA Translation: CAB52158.1
PIRiS58882
RefSeqiNP_587941.1, NM_001022932.2

Genome annotation databases

EnsemblFungiiSPCC18B5.11c.1; SPCC18B5.11c.1:pep; SPCC18B5.11c
GeneIDi2538959
KEGGispo:SPCC18B5.11c

Similar proteinsi

Entry informationi

Entry nameiCDS1_SCHPO
AccessioniPrimary (citable) accession number: Q09170
Secondary accession number(s): O42642
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 11, 2001
Last modified: March 28, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome