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Protein

Serine/arginine-rich splicing factor 5

Gene

Srsf5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be required for progression through G1 and entry into S phase of cell growth. May play a regulatory role in pre-mRNA splicing. Autoregulates its own expression. Plays a role in constitutive splicing and can modulate the selection of alternative splice sites (By similarity). Could play an important role in development and differentiation in the spleen and thymus.By similarity

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: Ensembl
  • protein kinase B binding Source: RGD
  • RNA binding Source: RGD

GO - Biological processi

  • cellular response to insulin stimulus Source: RGD
  • liver development Source: RGD
  • liver regeneration Source: RGD
  • mRNA processing Source: UniProtKB-KW
  • positive regulation of RNA splicing Source: RGD
  • regulation of cell cycle Source: RGD
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • response to insulin Source: RGD
  • response to wounding Source: Ensembl
  • RNA splicing Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA splicing, Transcription, Transcription regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-RNO-109688. Cleavage of Growing Transcript in the Termination Region.
R-RNO-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-RNO-72163. mRNA Splicing - Major Pathway.
R-RNO-72187. mRNA 3'-end processing.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich splicing factor 5
Alternative name(s):
Delayed-early protein HRS
Insulin-induced growth response protein CL-4
Pre-mRNA-splicing factor SRP40
Splicing factor, arginine/serine-rich 5
Gene namesi
Name:Srsf5
Synonyms:Cl-4, Hrs, Sfrs5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi3664. Srsf5.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: Ensembl
  • nuclear speck Source: UniProtKB
  • nucleolus Source: Ensembl
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000819291 – 269Serine/arginine-rich splicing factor 5Add BLAST269

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei86PhosphoserineBy similarity1
Modified residuei167N6-acetyllysineBy similarity1
Modified residuei224PhosphoserineBy similarity1
Modified residuei226PhosphoserineBy similarity1
Modified residuei230PhosphoserineBy similarity1
Modified residuei247PhosphoserineBy similarity1
Modified residuei250PhosphoserineCombined sources1

Post-translational modificationi

Extensively phosphorylated on serine residues in the RS domain.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ09167.
PRIDEiQ09167.

PTM databases

iPTMnetiQ09167.
PhosphoSitePlusiQ09167.

Expressioni

Tissue specificityi

Highly expressed in spleen and thymus.

Inductioni

By insulin and hepatectomy.

Gene expression databases

BgeeiENSRNOG00000005513.

Interactioni

Subunit structurei

Found in a pre-mRNA splicing complex with SRSF4/SFRS4, SRSF5/SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Interacts (via RS domain) with PHF5A (via N-terminus) (By similarity).By similarity

GO - Molecular functioni

  • protein kinase B binding Source: RGD

Protein-protein interaction databases

BioGridi248288. 1 interactor.
STRINGi10116.ENSRNOP00000007583.

Structurei

3D structure databases

ProteinModelPortaliQ09167.
SMRiQ09167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 74RRM 1PROSITE-ProRule annotationAdd BLAST71
Domaini108 – 181RRM 2PROSITE-ProRule annotationAdd BLAST74

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi75 – 107Arg/Gly-rich (hinge region)Add BLAST33
Compositional biasi184 – 264Arg/Ser-rich (RS domain)Add BLAST81

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated
Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0106. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00700000104103.
HOVERGENiHBG002295.
InParanoidiQ09167.
KOiK12893.
OMAiSHSPKSH.
OrthoDBiEOG091G0R9W.
PhylomeDBiQ09167.
TreeFamiTF351335.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q09167-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGCRVFIGR LNPAAREKDV ERFFKGYGRI RDIDLKRGFG FVEFEDPRDA
60 70 80 90 100
DDAVYELDGK ELCSERVTIE HARARSRGGR GRGRYSDRFS SRRPRNDRRN
110 120 130 140 150
APPVRTENRL IVENLSSRVS WQDLKDFMRQ AGEVTFADAH RPKLNEGVVE
160 170 180 190 200
FASYGDLKNA IEKLSGKEIN GRKIKLIEGS KRHRSRSRSR SRTRSSSRSR
210 220 230 240 250
SRSRSRRSKS YSRSRSRSRS RSKSRSGSRS PVPEKSQKRG SSSRSKSPAS
260
VDRQRSRSRS RSRSVDSGN
Length:269
Mass (Da):30,891
Last modified:November 1, 1995 - v1
Checksum:i0FC90628B64DE845
GO
Isoform 2 (identifier: Q09167-2) [UniParc]FASTAAdd to basket
Also known as: HRR-LF

The sequence of this isoform differs from the canonical sequence as follows:
     123-124: DL → VC
     125-269: Missing.

Show »
Length:124
Mass (Da):14,437
Checksum:iA4134DBC1C6133E2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti69I → M in AAB71864 (PubMed:9434190).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005867123 – 124DL → VC in isoform 2. 1 Publication2
Alternative sequenceiVSP_005868125 – 269Missing in isoform 2. 1 PublicationAdd BLAST145

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13635 mRNA. Translation: AAA62266.1.
AF020683 mRNA. Translation: AAB71864.1.
BC058479 mRNA. Translation: AAH58479.1.
L33267 mRNA. Translation: AAA42316.1.
PIRiB47112.
RefSeqiNP_001182434.1. NM_001195505.1. [Q09167-1]
NP_001182435.1. NM_001195506.1. [Q09167-1]
NP_062130.2. NM_019257.3. [Q09167-1]
UniGeneiRn.54448.

Genome annotation databases

EnsembliENSRNOT00000007583; ENSRNOP00000007583; ENSRNOG00000005513. [Q09167-1]
GeneIDi29667.
KEGGirno:29667.
UCSCiRGD:3664. rat. [Q09167-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13635 mRNA. Translation: AAA62266.1.
AF020683 mRNA. Translation: AAB71864.1.
BC058479 mRNA. Translation: AAH58479.1.
L33267 mRNA. Translation: AAA42316.1.
PIRiB47112.
RefSeqiNP_001182434.1. NM_001195505.1. [Q09167-1]
NP_001182435.1. NM_001195506.1. [Q09167-1]
NP_062130.2. NM_019257.3. [Q09167-1]
UniGeneiRn.54448.

3D structure databases

ProteinModelPortaliQ09167.
SMRiQ09167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248288. 1 interactor.
STRINGi10116.ENSRNOP00000007583.

PTM databases

iPTMnetiQ09167.
PhosphoSitePlusiQ09167.

Proteomic databases

PaxDbiQ09167.
PRIDEiQ09167.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007583; ENSRNOP00000007583; ENSRNOG00000005513. [Q09167-1]
GeneIDi29667.
KEGGirno:29667.
UCSCiRGD:3664. rat. [Q09167-1]

Organism-specific databases

CTDi6430.
RGDi3664. Srsf5.

Phylogenomic databases

eggNOGiKOG0106. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00700000104103.
HOVERGENiHBG002295.
InParanoidiQ09167.
KOiK12893.
OMAiSHSPKSH.
OrthoDBiEOG091G0R9W.
PhylomeDBiQ09167.
TreeFamiTF351335.

Enzyme and pathway databases

ReactomeiR-RNO-109688. Cleavage of Growing Transcript in the Termination Region.
R-RNO-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-RNO-72163. mRNA Splicing - Major Pathway.
R-RNO-72187. mRNA 3'-end processing.

Miscellaneous databases

PROiQ09167.

Gene expression databases

BgeeiENSRNOG00000005513.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRSF5_RAT
AccessioniPrimary (citable) accession number: Q09167
Secondary accession number(s): O35335
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.