Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q09163

- DLK1_MOUSE

UniProt

Q09163 - DLK1_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Protein delta homolog 1

Gene

Dlk1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation.2 Publications

GO - Molecular functioni

  1. calcium ion binding Source: InterPro

GO - Biological processi

  1. embryonic skeletal system development Source: MGI
  2. negative regulation of Notch signaling pathway Source: MGI
  3. post-embryonic development Source: MGI
  4. regulation of gene expression Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_199384. Activated NOTCH1 Transmits Signal to the Nucleus.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein delta homolog 1
Short name:
DLK-1
Alternative name(s):
Adipocyte differentiation inhibitor protein
Preadipocyte factor 1
Short name:
Pref-1
Cleaved into the following chain:
Fetal antigen 1
Short name:
FA1
Gene namesi
Name:Dlk1
Synonyms:Dlk, Pref1, Scp-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:94900. Dlk1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 305282ExtracellularSequence AnalysisAdd
BLAST
Transmembranei306 – 32924HelicalSequence AnalysisAdd
BLAST
Topological domaini330 – 38556CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. external side of plasma membrane Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323By similarityAdd
BLAST
Chaini24 – 385362Protein delta homolog 1PRO_0000007520Add
BLAST
Chaini24 – 305282Fetal antigen 1PRO_0000007521Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi26 ↔ 37PROSITE-ProRule annotation
Disulfide bondi30 ↔ 43PROSITE-ProRule annotation
Disulfide bondi45 ↔ 54PROSITE-ProRule annotation
Disulfide bondi57 ↔ 68PROSITE-ProRule annotation
Disulfide bondi63 ↔ 74PROSITE-ProRule annotation
Disulfide bondi76 ↔ 85PROSITE-ProRule annotation
Disulfide bondi92 ↔ 103PROSITE-ProRule annotation
Glycosylationi94 – 941O-linked (GalNAc...)1 PublicationCAR_000160
Disulfide bondi97 ↔ 113PROSITE-ProRule annotation
Glycosylationi100 – 1001N-linked (GlcNAc...)1 PublicationCAR_000183
Disulfide bondi115 ↔ 124PROSITE-ProRule annotation
Disulfide bondi131 ↔ 144PROSITE-ProRule annotation
Disulfide bondi138 ↔ 156PROSITE-ProRule annotation
Disulfide bondi158 ↔ 167PROSITE-ProRule annotation
Glycosylationi165 – 1651N-linked (GlcNAc...); partial; atypical1 Publication
Glycosylationi174 – 1741N-linked (GlcNAc...); atypical1 Publication
Disulfide bondi176 ↔ 187PROSITE-ProRule annotation
Disulfide bondi181 ↔ 196PROSITE-ProRule annotation
Disulfide bondi198 ↔ 207PROSITE-ProRule annotation
Disulfide bondi214 ↔ 225PROSITE-ProRule annotation
Glycosylationi216 – 2161O-linked (GalNAc...)1 PublicationCAR_000161
Disulfide bondi219 ↔ 235PROSITE-ProRule annotation
Glycosylationi224 – 2241O-linked (GalNAc...)1 PublicationCAR_000162
Disulfide bondi237 ↔ 246PROSITE-ProRule annotation
Glycosylationi258 – 2581O-linked (GalNAc...)1 Publication
Glycosylationi267 – 2671O-linked (GalNAc...); partial1 Publication
Glycosylationi271 – 2711O-linked (GalNAc...)1 Publication
Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N- and O-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ09163.
PRIDEiQ09163.

PTM databases

PhosphoSiteiQ09163.
UniCarbKBiQ09163.

Expressioni

Tissue specificityi

Highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary and, to a lesser degree, in adult kidney, muscle, thymus and heart.1 Publication

Developmental stagei

Expression is elevated in liver after birth but starts to decline around postnatal day 16.1 Publication

Gene expression databases

CleanExiMM_DLK1.
GenevestigatoriQ09163.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

DIPiDIP-6010N.
IntActiQ09163. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ09163.
SMRiQ09163. Positions 22-246.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 5532EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini53 – 8634EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini88 – 12538EGF-like 3PROSITE-ProRule annotationAdd
BLAST
Domaini127 – 16842EGF-like 4PROSITE-ProRule annotationAdd
BLAST
Domaini172 – 20837EGF-like 5PROSITE-ProRule annotationAdd
BLAST
Domaini210 – 24738EGF-like 6PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 6 EGF-like domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG306005.
HOGENOMiHOG000072581.
HOVERGENiHBG007065.
InParanoidiQ09163.
PhylomeDBiQ09163.

Family and domain databases

InterProiIPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
[Graphical view]
PfamiPF00008. EGF. 4 hits.
[Graphical view]
SMARTiSM00181. EGF. 5 hits.
SM00179. EGF_CA. 1 hit.
[Graphical view]
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS00022. EGF_1. 5 hits.
PS01186. EGF_2. 6 hits.
PS50026. EGF_3. 6 hits.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform A (identifier: Q09163-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIATGALLRV LLLLLAFGHS TYGAECDPPC DPQYGFCEAD NVCRCHVGWE
60 70 80 90 100
GPLCDKCVTA PGCVNGVCKE PWQCICKDGW DGKFCEIDVR ACTSTPCANN
110 120 130 140 150
GTCVDLEKGQ YECSCTPGFS GKDCQHKAGP CVINGSPCQH GGACVDDEGQ
160 170 180 190 200
ASHASCLCPP GFSGNFCEIV AATNSCTPNP CENDGVCTDI GGDFRCRCPA
210 220 230 240 250
GFVDKTCSRP VSNCASGPCQ NGGTCLQHTQ VSFECLCKPP FMGPTCAKKR
260 270 280 290 300
GASPVQVTHL PSGYGLTYRL TPGVHELPVQ QPEQHILKVS MKELNKSTPL
310 320 330 340 350
LTEGQAICFT ILGVLTSLVV LGTVAIVFLN KCETWVSNLR YNHTFRKKKN
360 370 380
LLLQYNSGEE LAVNIIFPEK IDMTTFNKEA GDEEI
Length:385
Mass (Da):41,320
Last modified:November 1, 1995 - v1
Checksum:iE79864FEA5AF4FF1
GO
Isoform B (identifier: Q09163-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     231-281: Missing.

Show »
Length:334
Mass (Da):35,811
Checksum:iCAEDBE46F3361527
GO
Isoform C (identifier: Q09163-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     231-303: Missing.

Show »
Length:312
Mass (Da):33,293
Checksum:i9201205F68663CC4
GO
Isoform C2 (identifier: Q09163-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     231-305: Missing.

Show »
Length:310
Mass (Da):33,108
Checksum:i0BDD15CDC082E4AD
GO
Isoform D (identifier: Q09163-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     211-303: Missing.

Show »
Length:292
Mass (Da):31,306
Checksum:i25CC1D603930C4EC
GO
Isoform D2 (identifier: Q09163-6) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     211-305: Missing.

Show »
Length:290
Mass (Da):31,121
Checksum:iE6E2449B38AA267E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti250 – 2501R → P in AAA37175. (PubMed:8500166)Curated
Sequence conflicti320 – 38566VLGTV…GDEEI → CWAPWPSSFSTSAKPGCPTC ATTTCFARRRTSCCSITAAR SWRSISSSPRRLT in AAA37175. (PubMed:8500166)CuratedAdd
BLAST
Sequence conflicti344 – 3452TF → ML in BAA04121. 1 PublicationCurated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti347 – 3471Missing.1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei211 – 30595Missing in isoform D2. CuratedVSP_001379Add
BLAST
Alternative sequencei211 – 30393Missing in isoform D. CuratedVSP_001378Add
BLAST
Alternative sequencei231 – 30575Missing in isoform C2. CuratedVSP_001382Add
BLAST
Alternative sequencei231 – 30373Missing in isoform C. CuratedVSP_001381Add
BLAST
Alternative sequencei231 – 28151Missing in isoform B. CuratedVSP_001380Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12171 mRNA. Translation: CAA78162.1.
U15980 mRNA. Translation: AAB60495.1.
L12721 mRNA. Translation: AAA37175.1.
S71340 Genomic DNA. No translation available.
D16847 mRNA. Translation: BAA04121.1.
CCDSiCCDS26168.1. [Q09163-1]
CCDS49173.1. [Q09163-6]
CCDS49174.1. [Q09163-2]
CCDS56863.1. [Q09163-3]
PIRiA54785.
S53718.
RefSeqiNP_034182.2. NM_010052.5.
UniGeneiMm.157069.

Genome annotation databases

GeneIDi13386.
KEGGimmu:13386.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12171 mRNA. Translation: CAA78162.1 .
U15980 mRNA. Translation: AAB60495.1 .
L12721 mRNA. Translation: AAA37175.1 .
S71340 Genomic DNA. No translation available.
D16847 mRNA. Translation: BAA04121.1 .
CCDSi CCDS26168.1. [Q09163-1 ]
CCDS49173.1. [Q09163-6 ]
CCDS49174.1. [Q09163-2 ]
CCDS56863.1. [Q09163-3 ]
PIRi A54785.
S53718.
RefSeqi NP_034182.2. NM_010052.5.
UniGenei Mm.157069.

3D structure databases

ProteinModelPortali Q09163.
SMRi Q09163. Positions 22-246.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-6010N.
IntActi Q09163. 1 interaction.

PTM databases

PhosphoSitei Q09163.
UniCarbKBi Q09163.

Proteomic databases

PaxDbi Q09163.
PRIDEi Q09163.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 13386.
KEGGi mmu:13386.

Organism-specific databases

CTDi 8788.
MGIi MGI:94900. Dlk1.

Phylogenomic databases

eggNOGi NOG306005.
HOGENOMi HOG000072581.
HOVERGENi HBG007065.
InParanoidi Q09163.
PhylomeDBi Q09163.

Enzyme and pathway databases

Reactomei REACT_199384. Activated NOTCH1 Transmits Signal to the Nucleus.

Miscellaneous databases

NextBioi 283744.
PROi Q09163.
SOURCEi Search...

Gene expression databases

CleanExi MM_DLK1.
Genevestigatori Q09163.

Family and domain databases

InterProi IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
[Graphical view ]
Pfami PF00008. EGF. 4 hits.
[Graphical view ]
SMARTi SM00181. EGF. 5 hits.
SM00179. EGF_CA. 1 hit.
[Graphical view ]
PROSITEi PS00010. ASX_HYDROXYL. 1 hit.
PS00022. EGF_1. 5 hits.
PS01186. EGF_2. 6 hits.
PS50026. EGF_3. 6 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "dlk, a putative mammalian homeotic gene differentially expressed in small cell lung carcinoma and neuroendocrine tumor cell line."
    Laborda J., Sausville E.A., Hoffman T., Notario V.
    J. Biol. Chem. 268:3817-3820(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: Swiss.
    Tissue: Fibroblast.
  2. "Pref-1, a protein containing EGF-like repeats, inhibits adipocyte differentiation."
    Smas C.M., Sul H.S.
    Cell 73:725-734(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  3. "dlk, pG2 and Pref-1 mRNAs encode similar proteins belonging to the EGF-like superfamily. Identification of polymorphic variants of this RNA."
    Lee Y.L., Helman L., Hoffman T., Laborda J.
    Biochim. Biophys. Acta 1261:223-232(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT LYS-347 DEL.
    Tissue: Adrenal gland and Placenta.
  4. Maruyama K., Nishijima S., Kuromitsu S., Ichikawa A., Masuda E., Takemoto T., Kodama H., Kawashima H.
    Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "Structural characterization and alternate splicing of the gene encoding the preadipocyte EGF-like protein pref-1."
    Smas C.M., Green D., Sul H.S.
    Biochemistry 33:9257-9265(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-8, ALTERNATIVE SPLICING.
  6. "Glycosylation analysis and protein structure determination of murine fetal antigen 1 (mFA1) -- the circulating gene product of the delta-like protein (dlk), preadipocyte factor 1 (Pref-1) and stromal-cell-derived protein 1 (SCP-1) cDNAs."
    Krogh T.N., Bachmann E., Teisner B., Skjoedt K., Hoejrup P.
    Eur. J. Biochem. 244:334-342(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT SER-94; ASN-100; ASN-165; ASN-174; SER-216; THR-224; THR-258; THR-267 AND THR-271, STRUCTURE OF CARBOHYDRATE.
  7. Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiDLK1_MOUSE
AccessioniPrimary (citable) accession number: Q09163
Secondary accession number(s): Q07645, Q62208
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 26, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3