Protein delta homolog 1 precursor - Mus musculus (Mouse)

Names and origin

Protein names

Recommended name:
    Protein delta homolog 1
      Short name=DLK-1
Alternative name(s):
    Preadipocyte factor 1
      Short name=Pref-1
    Adipocyte differentiation inhibitor protein
Cleaved into the following chain:
    1- Recommended name:
            Fetal antigen 1
                Short name=FA1

Gene names

Name:	Dlk1
Synonyms:	Dlk, Pref1, Scp-1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	May have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation.
Subunit structure	Monomer.
Subcellular location	Membrane; Single-pass type I membrane protein.
Post-translational modification	N- and O-glycosylated. Ref.6
Sequence similarities	Contains 6 EGF-like domains.

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Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	EGF-like domain Repeat Signal Transmembrane
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	embryonic skeletal system development Inferred from direct assay. Source: MGI post-embryonic development Inferred from direct assay. Source: MGI
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
Irak1	Q62406-1	2	EBI-646470,EBI-488313

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Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select] Note: Additional isoforms seem to exist.

Isoform A (identifier: Q09163-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: Q09163-2) The sequence of this isoform differs from the canonical sequence as follows: 231-281: Missing.

Isoform C (identifier: Q09163-3) The sequence of this isoform differs from the canonical sequence as follows: 231-303: Missing.

Isoform C2 (identifier: Q09163-4) The sequence of this isoform differs from the canonical sequence as follows: 231-305: Missing.

Isoform D (identifier: Q09163-5) The sequence of this isoform differs from the canonical sequence as follows: 211-303: Missing.

Isoform D2 (identifier: Q09163-6) The sequence of this isoform differs from the canonical sequence as follows: 211-305: Missing.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 23

23

By similarity

Chain

24 – 385

362

Protein delta homolog 1

PRO_0000007520

Chain

24 – 305

282

Fetal antigen 1

PRO_0000007521

Regions

Topological domain

24 – 305

282

Extracellular Potential

Transmembrane

306 – 329

24

Potential

Topological domain

330 – 385

56

Cytoplasmic Potential

Domain

24 – 55

32

EGF-like 1

Domain

53 – 86

34

EGF-like 2

Domain

88 – 125

38

EGF-like 3

Domain

127 – 168

42

EGF-like 4

Domain

172 – 208

37

EGF-like 5

Domain

210 – 247

38

EGF-like 6

Amino acid modifications

Glycosylation

94

1

O-linked (GalNAc...) Ref.6

CAR_000160

Glycosylation

100

1

N-linked (GlcNAc...) Ref.6

CAR_000183

Glycosylation

165

1

N-linked (GlcNAc...); partial; atypical Ref.6

Glycosylation

174

1

N-linked (GlcNAc...); atypical Ref.6

Glycosylation

216

1

O-linked (GalNAc...) Ref.6

CAR_000161

Glycosylation

224

1

O-linked (GalNAc...) Ref.6

CAR_000162

Glycosylation

258

1

O-linked (GalNAc...) Ref.6

Glycosylation

267

1

O-linked (GalNAc...); partial Ref.6

Glycosylation

271

1

O-linked (GalNAc...) Ref.6

Glycosylation

295

1

N-linked (GlcNAc...) Potential

Disulfide bond

26 ↔ 37

By similarity

Disulfide bond

30 ↔ 43

By similarity

Disulfide bond

45 ↔ 54

By similarity

Disulfide bond

57 ↔ 68

By similarity

Disulfide bond

63 ↔ 74

By similarity

Disulfide bond

76 ↔ 85

By similarity

Disulfide bond

92 ↔ 103

By similarity

Disulfide bond

97 ↔ 113

By similarity

Disulfide bond

115 ↔ 124

By similarity

Disulfide bond

131 ↔ 144

By similarity

Disulfide bond

138 ↔ 156

By similarity

Disulfide bond

158 ↔ 167

By similarity

Disulfide bond

176 ↔ 187

By similarity

Disulfide bond

181 ↔ 196

By similarity

Disulfide bond

198 ↔ 207

By similarity

Disulfide bond

214 ↔ 225

By similarity

Disulfide bond

219 ↔ 235

By similarity

Disulfide bond

237 ↔ 246

By similarity

Natural variations

Alternative sequence

211 – 305

95

Missing in isoform D2.

VSP_001379

Alternative sequence

211 – 303

93

Missing in isoform D.

VSP_001378

Alternative sequence

231 – 305

75

Missing in isoform C2.

VSP_001382

Alternative sequence

231 – 303

73

Missing in isoform C.

VSP_001381

Alternative sequence

231 – 281

51

Missing in isoform B.

VSP_001380

Natural variant

347

1

Missing Ref.3

Experimental info

Sequence conflict

250

1

R → P in AAA37175. Ref.2

Sequence conflict

320 – 385

66

VLGTV…GDEEI → CWAPWPSSFSTSAKPGCPTC ATTTCFARRRTSCCSITAAR SWRSISSSPRRLT in AAA37175. Ref.2

Sequence conflict

344 – 345

2

TF → ML in BAA04121. Ref.4

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Sequences

Sequence		Length	Mass (Da)
Isoform A [UniParc]. Last modified November 1, 1995. Version 1. Checksum: E79864FEA5AF4FF1 Show »	FASTA	385	41,320
10 20 30 40 50 60 MIATGALLRV LLLLLAFGHS TYGAECDPPC DPQYGFCEAD NVCRCHVGWE GPLCDKCVTA 70 80 90 100 110 120 PGCVNGVCKE PWQCICKDGW DGKFCEIDVR ACTSTPCANN GTCVDLEKGQ YECSCTPGFS 130 140 150 160 170 180 GKDCQHKAGP CVINGSPCQH GGACVDDEGQ ASHASCLCPP GFSGNFCEIV AATNSCTPNP 190 200 210 220 230 240 CENDGVCTDI GGDFRCRCPA GFVDKTCSRP VSNCASGPCQ NGGTCLQHTQ VSFECLCKPP 250 260 270 280 290 300 FMGPTCAKKR GASPVQVTHL PSGYGLTYRL TPGVHELPVQ QPEQHILKVS MKELNKSTPL 310 320 330 340 350 360 LTEGQAICFT ILGVLTSLVV LGTVAIVFLN KCETWVSNLR YNHTFRKKKN LLLQYNSGEE 370 380 LAVNIIFPEK IDMTTFNKEA GDEEI « Hide
Isoform B. Checksum: CAEDBE46F3361527 Show »	FASTA	334	35,811
10 20 30 40 50 60 MIATGALLRV LLLLLAFGHS TYGAECDPPC DPQYGFCEAD NVCRCHVGWE GPLCDKCVTA 70 80 90 100 110 120 PGCVNGVCKE PWQCICKDGW DGKFCEIDVR ACTSTPCANN GTCVDLEKGQ YECSCTPGFS 130 140 150 160 170 180 GKDCQHKAGP CVINGSPCQH GGACVDDEGQ ASHASCLCPP GFSGNFCEIV AATNSCTPNP 190 200 210 220 230 240 CENDGVCTDI GGDFRCRCPA GFVDKTCSRP VSNCASGPCQ NGGTCLQHTQ PEQHILKVSM 250 260 270 280 290 300 KELNKSTPLL TEGQAICFTI LGVLTSLVVL GTVAIVFLNK CETWVSNLRY NHTFRKKKNL 310 320 330 LLQYNSGEEL AVNIIFPEKI DMTTFNKEAG DEEI « Hide
Isoform C. Checksum: 9201205F68663CC4 Show »	FASTA	312	33,293
10 20 30 40 50 60 MIATGALLRV LLLLLAFGHS TYGAECDPPC DPQYGFCEAD NVCRCHVGWE GPLCDKCVTA 70 80 90 100 110 120 PGCVNGVCKE PWQCICKDGW DGKFCEIDVR ACTSTPCANN GTCVDLEKGQ YECSCTPGFS 130 140 150 160 170 180 GKDCQHKAGP CVINGSPCQH GGACVDDEGQ ASHASCLCPP GFSGNFCEIV AATNSCTPNP 190 200 210 220 230 240 CENDGVCTDI GGDFRCRCPA GFVDKTCSRP VSNCASGPCQ NGGTCLQHTQ GQAICFTILG 250 260 270 280 290 300 VLTSLVVLGT VAIVFLNKCE TWVSNLRYNH TFRKKKNLLL QYNSGEELAV NIIFPEKIDM 310 TTFNKEAGDE EI « Hide
Isoform C2. Checksum: 0BDD15CDC082E4AD Show »	FASTA	310	33,108
10 20 30 40 50 60 MIATGALLRV LLLLLAFGHS TYGAECDPPC DPQYGFCEAD NVCRCHVGWE GPLCDKCVTA 70 80 90 100 110 120 PGCVNGVCKE PWQCICKDGW DGKFCEIDVR ACTSTPCANN GTCVDLEKGQ YECSCTPGFS 130 140 150 160 170 180 GKDCQHKAGP CVINGSPCQH GGACVDDEGQ ASHASCLCPP GFSGNFCEIV AATNSCTPNP 190 200 210 220 230 240 CENDGVCTDI GGDFRCRCPA GFVDKTCSRP VSNCASGPCQ NGGTCLQHTQ AICFTILGVL 250 260 270 280 290 300 TSLVVLGTVA IVFLNKCETW VSNLRYNHTF RKKKNLLLQY NSGEELAVNI IFPEKIDMTT 310 FNKEAGDEEI « Hide
Isoform D. Checksum: 25CC1D603930C4EC Show »	FASTA	292	31,306
10 20 30 40 50 60 MIATGALLRV LLLLLAFGHS TYGAECDPPC DPQYGFCEAD NVCRCHVGWE GPLCDKCVTA 70 80 90 100 110 120 PGCVNGVCKE PWQCICKDGW DGKFCEIDVR ACTSTPCANN GTCVDLEKGQ YECSCTPGFS 130 140 150 160 170 180 GKDCQHKAGP CVINGSPCQH GGACVDDEGQ ASHASCLCPP GFSGNFCEIV AATNSCTPNP 190 200 210 220 230 240 CENDGVCTDI GGDFRCRCPA GFVDKTCSRP GQAICFTILG VLTSLVVLGT VAIVFLNKCE 250 260 270 280 290 TWVSNLRYNH TFRKKKNLLL QYNSGEELAV NIIFPEKIDM TTFNKEAGDE EI « Hide
Isoform D2. Checksum: E6E2449B38AA267E Show »	FASTA	290	31,121
10 20 30 40 50 60 MIATGALLRV LLLLLAFGHS TYGAECDPPC DPQYGFCEAD NVCRCHVGWE GPLCDKCVTA 70 80 90 100 110 120 PGCVNGVCKE PWQCICKDGW DGKFCEIDVR ACTSTPCANN GTCVDLEKGQ YECSCTPGFS 130 140 150 160 170 180 GKDCQHKAGP CVINGSPCQH GGACVDDEGQ ASHASCLCPP GFSGNFCEIV AATNSCTPNP 190 200 210 220 230 240 CENDGVCTDI GGDFRCRCPA GFVDKTCSRP AICFTILGVL TSLVVLGTVA IVFLNKCETW 250 260 270 280 290 VSNLRYNHTF RKKKNLLLQY NSGEELAVNI IFPEKIDMTT FNKEAGDEEI « Hide

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References

[1]	"dlk, a putative mammalian homeotic gene differentially expressed in small cell lung carcinoma and neuroendocrine tumor cell line." Laborda J., Sausville E.A., Hoffman T., Notario V. J. Biol. Chem. 268:3817-3820(1993) [PubMed: 8095043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Swiss. Tissue: Fibroblast.
[2]	"Pref-1, a protein containing EGF-like repeats, inhibits adipocyte differentiation." Smas C.M., Sul H.S. Cell 73:725-734(1993) [PubMed: 8500166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"dlk, pG2 and Pref-1 mRNAs encode similar proteins belonging to the EGF-like superfamily. Identification of polymorphic variants of this RNA." Lee Y.L., Helman L., Hoffman T., Laborda J. Biochim. Biophys. Acta 1261:223-232(1995) [PubMed: 7711066] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT LYS-347 DEL. Tissue: Adrenal gland and Placenta.
[4]	Maruyama K., Nishijima S., Kuromitsu S., Ichikawa A., Masuda E., Takemoto T., Kodama H., Kawashima H. Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Structural characterization and alternate splicing of the gene encoding the preadipocyte EGF-like protein pref-1." Smas C.M., Green D., Sul H.S. Biochemistry 33:9257-9265(1994) [PubMed: 7519443] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-8, ALTERNATIVE SPLICING.
[6]	"Glycosylation analysis and protein structure determination of murine fetal antigen 1 (mFA1) -- the circulating gene product of the delta-like protein (dlk), preadipocyte factor 1 (Pref-1) and stromal-cell-derived protein 1 (SCP-1) cDNAs." Krogh T.N., Bachmann E., Teisner B., Skjoedt K., Hoejrup P. Eur. J. Biochem. 244:334-342(1997) [PubMed: 9118998] [Abstract] Cited for: GLYCOSYLATION AT SER-94; ASN-100; ASN-165; ASN-174; SER-216; THR-224; THR-258; THR-267 AND THR-271, STRUCTURE OF CARBOHYDRATE.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	Z12171 mRNA. Translation: CAA78162.1. U15980 mRNA. Translation: AAB60495.1. L12721 mRNA. Translation: AAA37175.1. S71340 Genomic DNA. No translation available. D16847 mRNA. Translation: BAA04121.1.
IPI	IPI00121716. IPI00226664. IPI00226665. IPI00321359. IPI00399832. IPI00918517.
PIR	A54785. S53718.
UniGene	Mm.157069
3D structure databases
HSSP	HSSP built from PDB template 1EDM based on UniProtKB P00740.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:6010N.
IntAct	Q09163. 1 interaction.
PTM databases
GlycoSuiteDB	Q09163.
Genome annotation databases
Ensembl	ENSMUSG00000040856. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:94900. Dlk1.
Phylogenomic databases
HOGENOM	Q09163.
HOVERGEN	Q09163.
Gene expression databases
ArrayExpress	Q09163.
Bgee	Q09163.
CleanEx	MM_DLK1.
GermOnline	ENSMUSG00000040856. Mus musculus.
Family and domain databases
InterPro	IPR006209. EGF. IPR006210. EGF-like. IPR013032. EGF-like_reg_CS. IPR000152. EGF-type_Asp/Asn_hydroxyl_CS. IPR001438. EGF_2. IPR000742. EGF_3. IPR001881. EGF_Ca_bd. [Graphical view]
Pfam	PF00008. EGF. 4 hits. [Graphical view]
PRINTS	PR00010. EGFBLOOD.
SMART	SM00181. EGF. 5 hits. SM00179. EGF_CA. 1 hit. [Graphical view]
PROSITE	PS00010. ASX_HYDROXYL. 1 hit. PS00022. EGF_1. 5 hits. PS01186. EGF_2. 6 hits. PS50026. EGF_3. 6 hits. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

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Entry information

Entry name

DLK1_MOUSE

Accession

Primary (citable) accession number: Q09163
Secondary accession number(s): Q07645, Q62208

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Alternative products

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents