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Q09163 (DLK1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein delta homolog 1

Short name=DLK-1
Alternative name(s):
Adipocyte differentiation inhibitor protein
Preadipocyte factor 1
Short name=Pref-1

Cleaved into the following chain:

  1. Fetal antigen 1
    Short name=FA1
Gene names
Name:Dlk1
Synonyms:Dlk, Pref1, Scp-1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length385 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation. Ref.1 Ref.2

Subunit structure

Monomer.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary and, to a lesser degree, in adult kidney, muscle, thymus and heart. Ref.7

Developmental stage

Expression is elevated in liver after birth but starts to decline around postnatal day 16. Ref.7

Post-translational modification

N- and O-glycosylated. Ref.6

Sequence similarities

Contains 6 EGF-like domains.

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform A (identifier: Q09163-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B (identifier: Q09163-2)

The sequence of this isoform differs from the canonical sequence as follows:
     231-281: Missing.
Isoform C (identifier: Q09163-3)

The sequence of this isoform differs from the canonical sequence as follows:
     231-303: Missing.
Isoform C2 (identifier: Q09163-4)

The sequence of this isoform differs from the canonical sequence as follows:
     231-305: Missing.
Isoform D (identifier: Q09163-5)

The sequence of this isoform differs from the canonical sequence as follows:
     211-303: Missing.
Isoform D2 (identifier: Q09163-6)

The sequence of this isoform differs from the canonical sequence as follows:
     211-305: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 By similarity
Chain24 – 385362Protein delta homolog 1
PRO_0000007520
Chain24 – 305282Fetal antigen 1
PRO_0000007521

Regions

Topological domain24 – 305282Extracellular Potential
Transmembrane306 – 32924Helical; Potential
Topological domain330 – 38556Cytoplasmic Potential
Domain24 – 5532EGF-like 1
Domain53 – 8634EGF-like 2
Domain88 – 12538EGF-like 3
Domain127 – 16842EGF-like 4
Domain172 – 20837EGF-like 5
Domain210 – 24738EGF-like 6

Amino acid modifications

Glycosylation941O-linked (GalNAc...) Ref.6
CAR_000160
Glycosylation1001N-linked (GlcNAc...) Ref.6
CAR_000183
Glycosylation1651N-linked (GlcNAc...); partial; atypical Ref.6
Glycosylation1741N-linked (GlcNAc...); atypical Ref.6
Glycosylation2161O-linked (GalNAc...) Ref.6
CAR_000161
Glycosylation2241O-linked (GalNAc...) Ref.6
CAR_000162
Glycosylation2581O-linked (GalNAc...) Ref.6
Glycosylation2671O-linked (GalNAc...); partial Ref.6
Glycosylation2711O-linked (GalNAc...) Ref.6
Glycosylation2951N-linked (GlcNAc...) Potential
Disulfide bond26 ↔ 37 By similarity
Disulfide bond30 ↔ 43 By similarity
Disulfide bond45 ↔ 54 By similarity
Disulfide bond57 ↔ 68 By similarity
Disulfide bond63 ↔ 74 By similarity
Disulfide bond76 ↔ 85 By similarity
Disulfide bond92 ↔ 103 By similarity
Disulfide bond97 ↔ 113 By similarity
Disulfide bond115 ↔ 124 By similarity
Disulfide bond131 ↔ 144 By similarity
Disulfide bond138 ↔ 156 By similarity
Disulfide bond158 ↔ 167 By similarity
Disulfide bond176 ↔ 187 By similarity
Disulfide bond181 ↔ 196 By similarity
Disulfide bond198 ↔ 207 By similarity
Disulfide bond214 ↔ 225 By similarity
Disulfide bond219 ↔ 235 By similarity
Disulfide bond237 ↔ 246 By similarity

Natural variations

Alternative sequence211 – 30595Missing in isoform D2.
VSP_001379
Alternative sequence211 – 30393Missing in isoform D.
VSP_001378
Alternative sequence231 – 30575Missing in isoform C2.
VSP_001382
Alternative sequence231 – 30373Missing in isoform C.
VSP_001381
Alternative sequence231 – 28151Missing in isoform B.
VSP_001380
Natural variant3471Missing. Ref.3

Experimental info

Sequence conflict2501R → P in AAA37175. Ref.2
Sequence conflict320 – 38566VLGTV…GDEEI → CWAPWPSSFSTSAKPGCPTC ATTTCFARRRTSCCSITAAR SWRSISSSPRRLT in AAA37175. Ref.2
Sequence conflict344 – 3452TF → ML in BAA04121. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: E79864FEA5AF4FF1

FASTA38541,320
        10         20         30         40         50         60 
MIATGALLRV LLLLLAFGHS TYGAECDPPC DPQYGFCEAD NVCRCHVGWE GPLCDKCVTA 

        70         80         90        100        110        120 
PGCVNGVCKE PWQCICKDGW DGKFCEIDVR ACTSTPCANN GTCVDLEKGQ YECSCTPGFS 

       130        140        150        160        170        180 
GKDCQHKAGP CVINGSPCQH GGACVDDEGQ ASHASCLCPP GFSGNFCEIV AATNSCTPNP 

       190        200        210        220        230        240 
CENDGVCTDI GGDFRCRCPA GFVDKTCSRP VSNCASGPCQ NGGTCLQHTQ VSFECLCKPP 

       250        260        270        280        290        300 
FMGPTCAKKR GASPVQVTHL PSGYGLTYRL TPGVHELPVQ QPEQHILKVS MKELNKSTPL 

       310        320        330        340        350        360 
LTEGQAICFT ILGVLTSLVV LGTVAIVFLN KCETWVSNLR YNHTFRKKKN LLLQYNSGEE 

       370        380 
LAVNIIFPEK IDMTTFNKEA GDEEI 

« Hide

Isoform B [UniParc].

Checksum: CAEDBE46F3361527
Show »

FASTA33435,811
Isoform C [UniParc].

Checksum: 9201205F68663CC4
Show »

FASTA31233,293
Isoform C2 [UniParc].

Checksum: 0BDD15CDC082E4AD
Show »

FASTA31033,108
Isoform D [UniParc].

Checksum: 25CC1D603930C4EC
Show »

FASTA29231,306
Isoform D2 [UniParc].

Checksum: E6E2449B38AA267E
Show »

FASTA29031,121

References

[1]"dlk, a putative mammalian homeotic gene differentially expressed in small cell lung carcinoma and neuroendocrine tumor cell line."
Laborda J., Sausville E.A., Hoffman T., Notario V.
J. Biol. Chem. 268:3817-3820(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
Strain: Swiss.
Tissue: Fibroblast.
[2]"Pref-1, a protein containing EGF-like repeats, inhibits adipocyte differentiation."
Smas C.M., Sul H.S.
Cell 73:725-734(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[3]"dlk, pG2 and Pref-1 mRNAs encode similar proteins belonging to the EGF-like superfamily. Identification of polymorphic variants of this RNA."
Lee Y.L., Helman L., Hoffman T., Laborda J.
Biochim. Biophys. Acta 1261:223-232(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT LYS-347 DEL.
Tissue: Adrenal gland and Placenta.
[4]Maruyama K., Nishijima S., Kuromitsu S., Ichikawa A., Masuda E., Takemoto T., Kodama H., Kawashima H.
Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]"Structural characterization and alternate splicing of the gene encoding the preadipocyte EGF-like protein pref-1."
Smas C.M., Green D., Sul H.S.
Biochemistry 33:9257-9265(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-8, ALTERNATIVE SPLICING.
[6]"Glycosylation analysis and protein structure determination of murine fetal antigen 1 (mFA1) -- the circulating gene product of the delta-like protein (dlk), preadipocyte factor 1 (Pref-1) and stromal-cell-derived protein 1 (SCP-1) cDNAs."
Krogh T.N., Bachmann E., Teisner B., Skjoedt K., Hoejrup P.
Eur. J. Biochem. 244:334-342(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT SER-94; ASN-100; ASN-165; ASN-174; SER-216; THR-224; THR-258; THR-267 AND THR-271, STRUCTURE OF CARBOHYDRATE.
[7]"The novel gene EGFL9/Dlk2, highly homologous to Dlk1, functions as a modulator of adipogenesis."
Nueda M.L., Baladron V., Garcia-Ramirez J.J., Sanchez-Solana B., Ruvira M.D., Rivero S., Ballesteros M.A., Monsalve E.M., Diaz-Guerra M.J., Ruiz-Hidalgo M.J., Laborda J.
J. Mol. Biol. 367:1270-1280(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z12171 mRNA. Translation: CAA78162.1.
U15980 mRNA. Translation: AAB60495.1.
L12721 mRNA. Translation: AAA37175.1.
S71340 Genomic DNA. No translation available.
D16847 mRNA. Translation: BAA04121.1.
CCDSCCDS26168.1. [Q09163-1]
CCDS49173.1. [Q09163-6]
CCDS49174.1. [Q09163-2]
CCDS56863.1. [Q09163-3]
PIRA54785.
S53718.
RefSeqNP_034182.2. NM_010052.5.
UniGeneMm.157069.

3D structure databases

ProteinModelPortalQ09163.
SMRQ09163. Positions 17-288.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-6010N.
IntActQ09163. 1 interaction.

PTM databases

PhosphoSiteQ09163.
UniCarbKBQ09163.

Proteomic databases

PaxDbQ09163.
PRIDEQ09163.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID13386.
KEGGmmu:13386.

Organism-specific databases

CTD8788.
MGIMGI:94900. Dlk1.

Phylogenomic databases

eggNOGNOG306005.
HOGENOMHOG000072581.
HOVERGENHBG007065.
InParanoidQ09163.
PhylomeDBQ09163.

Enzyme and pathway databases

ReactomeREACT_188257. Signal Transduction.
REACT_189085. Disease.

Gene expression databases

CleanExMM_DLK1.
GenevestigatorQ09163.

Family and domain databases

InterProIPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
[Graphical view]
PfamPF00008. EGF. 4 hits.
[Graphical view]
SMARTSM00181. EGF. 5 hits.
SM00179. EGF_CA. 1 hit.
[Graphical view]
PROSITEPS00010. ASX_HYDROXYL. 1 hit.
PS00022. EGF_1. 5 hits.
PS01186. EGF_2. 6 hits.
PS50026. EGF_3. 6 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSDLK1. mouse.
NextBio283744.
PROQ09163.
SOURCESearch...

Entry information

Entry nameDLK1_MOUSE
AccessionPrimary (citable) accession number: Q09163
Secondary accession number(s): Q07645, Q62208
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot