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Q09163

- DLK1_MOUSE

UniProt

Q09163 - DLK1_MOUSE

Protein

Protein delta homolog 1

Gene

Dlk1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    May have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation.2 Publications

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro

    GO - Biological processi

    1. embryonic skeletal system development Source: MGI
    2. negative regulation of Notch signaling pathway Source: MGI
    3. post-embryonic development Source: MGI
    4. regulation of gene expression Source: MGI

    Enzyme and pathway databases

    ReactomeiREACT_199384. Activated NOTCH1 Transmits Signal to the Nucleus.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein delta homolog 1
    Short name:
    DLK-1
    Alternative name(s):
    Adipocyte differentiation inhibitor protein
    Preadipocyte factor 1
    Short name:
    Pref-1
    Cleaved into the following chain:
    Fetal antigen 1
    Short name:
    FA1
    Gene namesi
    Name:Dlk1
    Synonyms:Dlk, Pref1, Scp-1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:94900. Dlk1.

    Subcellular locationi

    GO - Cellular componenti

    1. external side of plasma membrane Source: MGI
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323By similarityAdd
    BLAST
    Chaini24 – 385362Protein delta homolog 1PRO_0000007520Add
    BLAST
    Chaini24 – 305282Fetal antigen 1PRO_0000007521Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi26 ↔ 37PROSITE-ProRule annotation
    Disulfide bondi30 ↔ 43PROSITE-ProRule annotation
    Disulfide bondi45 ↔ 54PROSITE-ProRule annotation
    Disulfide bondi57 ↔ 68PROSITE-ProRule annotation
    Disulfide bondi63 ↔ 74PROSITE-ProRule annotation
    Disulfide bondi76 ↔ 85PROSITE-ProRule annotation
    Disulfide bondi92 ↔ 103PROSITE-ProRule annotation
    Glycosylationi94 – 941O-linked (GalNAc...)1 PublicationCAR_000160
    Disulfide bondi97 ↔ 113PROSITE-ProRule annotation
    Glycosylationi100 – 1001N-linked (GlcNAc...)1 PublicationCAR_000183
    Disulfide bondi115 ↔ 124PROSITE-ProRule annotation
    Disulfide bondi131 ↔ 144PROSITE-ProRule annotation
    Disulfide bondi138 ↔ 156PROSITE-ProRule annotation
    Disulfide bondi158 ↔ 167PROSITE-ProRule annotation
    Glycosylationi165 – 1651N-linked (GlcNAc...); partial; atypical1 Publication
    Glycosylationi174 – 1741N-linked (GlcNAc...); atypical1 Publication
    Disulfide bondi176 ↔ 187PROSITE-ProRule annotation
    Disulfide bondi181 ↔ 196PROSITE-ProRule annotation
    Disulfide bondi198 ↔ 207PROSITE-ProRule annotation
    Disulfide bondi214 ↔ 225PROSITE-ProRule annotation
    Glycosylationi216 – 2161O-linked (GalNAc...)1 PublicationCAR_000161
    Disulfide bondi219 ↔ 235PROSITE-ProRule annotation
    Glycosylationi224 – 2241O-linked (GalNAc...)1 PublicationCAR_000162
    Disulfide bondi237 ↔ 246PROSITE-ProRule annotation
    Glycosylationi258 – 2581O-linked (GalNAc...)1 Publication
    Glycosylationi267 – 2671O-linked (GalNAc...); partial1 Publication
    Glycosylationi271 – 2711O-linked (GalNAc...)1 Publication
    Glycosylationi295 – 2951N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N- and O-glycosylated.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ09163.
    PRIDEiQ09163.

    PTM databases

    PhosphoSiteiQ09163.
    UniCarbKBiQ09163.

    Expressioni

    Tissue specificityi

    Highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary and, to a lesser degree, in adult kidney, muscle, thymus and heart.1 Publication

    Developmental stagei

    Expression is elevated in liver after birth but starts to decline around postnatal day 16.1 Publication

    Gene expression databases

    CleanExiMM_DLK1.
    GenevestigatoriQ09163.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    DIPiDIP-6010N.
    IntActiQ09163. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ09163.
    SMRiQ09163. Positions 17-288.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini24 – 305282ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini330 – 38556CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei306 – 32924HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini24 – 5532EGF-like 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini53 – 8634EGF-like 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini88 – 12538EGF-like 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini127 – 16842EGF-like 4PROSITE-ProRule annotationAdd
    BLAST
    Domaini172 – 20837EGF-like 5PROSITE-ProRule annotationAdd
    BLAST
    Domaini210 – 24738EGF-like 6PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 6 EGF-like domains.PROSITE-ProRule annotation

    Keywords - Domaini

    EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG306005.
    HOGENOMiHOG000072581.
    HOVERGENiHBG007065.
    InParanoidiQ09163.
    PhylomeDBiQ09163.

    Family and domain databases

    InterProiIPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
    [Graphical view]
    PfamiPF00008. EGF. 4 hits.
    [Graphical view]
    SMARTiSM00181. EGF. 5 hits.
    SM00179. EGF_CA. 1 hit.
    [Graphical view]
    PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
    PS00022. EGF_1. 5 hits.
    PS01186. EGF_2. 6 hits.
    PS50026. EGF_3. 6 hits.
    [Graphical view]

    Sequences (6)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 6 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform A (identifier: Q09163-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MIATGALLRV LLLLLAFGHS TYGAECDPPC DPQYGFCEAD NVCRCHVGWE    50
    GPLCDKCVTA PGCVNGVCKE PWQCICKDGW DGKFCEIDVR ACTSTPCANN 100
    GTCVDLEKGQ YECSCTPGFS GKDCQHKAGP CVINGSPCQH GGACVDDEGQ 150
    ASHASCLCPP GFSGNFCEIV AATNSCTPNP CENDGVCTDI GGDFRCRCPA 200
    GFVDKTCSRP VSNCASGPCQ NGGTCLQHTQ VSFECLCKPP FMGPTCAKKR 250
    GASPVQVTHL PSGYGLTYRL TPGVHELPVQ QPEQHILKVS MKELNKSTPL 300
    LTEGQAICFT ILGVLTSLVV LGTVAIVFLN KCETWVSNLR YNHTFRKKKN 350
    LLLQYNSGEE LAVNIIFPEK IDMTTFNKEA GDEEI 385
    Length:385
    Mass (Da):41,320
    Last modified:November 1, 1995 - v1
    Checksum:iE79864FEA5AF4FF1
    GO
    Isoform B (identifier: Q09163-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         231-281: Missing.

    Show »
    Length:334
    Mass (Da):35,811
    Checksum:iCAEDBE46F3361527
    GO
    Isoform C (identifier: Q09163-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         231-303: Missing.

    Show »
    Length:312
    Mass (Da):33,293
    Checksum:i9201205F68663CC4
    GO
    Isoform C2 (identifier: Q09163-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         231-305: Missing.

    Show »
    Length:310
    Mass (Da):33,108
    Checksum:i0BDD15CDC082E4AD
    GO
    Isoform D (identifier: Q09163-5) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         211-303: Missing.

    Show »
    Length:292
    Mass (Da):31,306
    Checksum:i25CC1D603930C4EC
    GO
    Isoform D2 (identifier: Q09163-6) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         211-305: Missing.

    Show »
    Length:290
    Mass (Da):31,121
    Checksum:iE6E2449B38AA267E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti250 – 2501R → P in AAA37175. (PubMed:8500166)Curated
    Sequence conflicti320 – 38566VLGTV…GDEEI → CWAPWPSSFSTSAKPGCPTC ATTTCFARRRTSCCSITAAR SWRSISSSPRRLT in AAA37175. (PubMed:8500166)CuratedAdd
    BLAST
    Sequence conflicti344 – 3452TF → ML in BAA04121. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti347 – 3471Missing.1 Publication

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei211 – 30595Missing in isoform D2. CuratedVSP_001379Add
    BLAST
    Alternative sequencei211 – 30393Missing in isoform D. CuratedVSP_001378Add
    BLAST
    Alternative sequencei231 – 30575Missing in isoform C2. CuratedVSP_001382Add
    BLAST
    Alternative sequencei231 – 30373Missing in isoform C. CuratedVSP_001381Add
    BLAST
    Alternative sequencei231 – 28151Missing in isoform B. CuratedVSP_001380Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z12171 mRNA. Translation: CAA78162.1.
    U15980 mRNA. Translation: AAB60495.1.
    L12721 mRNA. Translation: AAA37175.1.
    S71340 Genomic DNA. No translation available.
    D16847 mRNA. Translation: BAA04121.1.
    CCDSiCCDS26168.1. [Q09163-1]
    CCDS49173.1. [Q09163-6]
    CCDS49174.1. [Q09163-2]
    CCDS56863.1. [Q09163-3]
    PIRiA54785.
    S53718.
    RefSeqiNP_034182.2. NM_010052.5.
    UniGeneiMm.157069.

    Genome annotation databases

    GeneIDi13386.
    KEGGimmu:13386.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z12171 mRNA. Translation: CAA78162.1 .
    U15980 mRNA. Translation: AAB60495.1 .
    L12721 mRNA. Translation: AAA37175.1 .
    S71340 Genomic DNA. No translation available.
    D16847 mRNA. Translation: BAA04121.1 .
    CCDSi CCDS26168.1. [Q09163-1 ]
    CCDS49173.1. [Q09163-6 ]
    CCDS49174.1. [Q09163-2 ]
    CCDS56863.1. [Q09163-3 ]
    PIRi A54785.
    S53718.
    RefSeqi NP_034182.2. NM_010052.5.
    UniGenei Mm.157069.

    3D structure databases

    ProteinModelPortali Q09163.
    SMRi Q09163. Positions 17-288.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-6010N.
    IntActi Q09163. 1 interaction.

    PTM databases

    PhosphoSitei Q09163.
    UniCarbKBi Q09163.

    Proteomic databases

    PaxDbi Q09163.
    PRIDEi Q09163.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 13386.
    KEGGi mmu:13386.

    Organism-specific databases

    CTDi 8788.
    MGIi MGI:94900. Dlk1.

    Phylogenomic databases

    eggNOGi NOG306005.
    HOGENOMi HOG000072581.
    HOVERGENi HBG007065.
    InParanoidi Q09163.
    PhylomeDBi Q09163.

    Enzyme and pathway databases

    Reactomei REACT_199384. Activated NOTCH1 Transmits Signal to the Nucleus.

    Miscellaneous databases

    ChiTaRSi DLK1. mouse.
    NextBioi 283744.
    PROi Q09163.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_DLK1.
    Genevestigatori Q09163.

    Family and domain databases

    InterProi IPR000742. EG-like_dom.
    IPR001881. EGF-like_Ca-bd_dom.
    IPR013032. EGF-like_CS.
    IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
    [Graphical view ]
    Pfami PF00008. EGF. 4 hits.
    [Graphical view ]
    SMARTi SM00181. EGF. 5 hits.
    SM00179. EGF_CA. 1 hit.
    [Graphical view ]
    PROSITEi PS00010. ASX_HYDROXYL. 1 hit.
    PS00022. EGF_1. 5 hits.
    PS01186. EGF_2. 6 hits.
    PS50026. EGF_3. 6 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "dlk, a putative mammalian homeotic gene differentially expressed in small cell lung carcinoma and neuroendocrine tumor cell line."
      Laborda J., Sausville E.A., Hoffman T., Notario V.
      J. Biol. Chem. 268:3817-3820(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
      Strain: Swiss.
      Tissue: Fibroblast.
    2. "Pref-1, a protein containing EGF-like repeats, inhibits adipocyte differentiation."
      Smas C.M., Sul H.S.
      Cell 73:725-734(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    3. "dlk, pG2 and Pref-1 mRNAs encode similar proteins belonging to the EGF-like superfamily. Identification of polymorphic variants of this RNA."
      Lee Y.L., Helman L., Hoffman T., Laborda J.
      Biochim. Biophys. Acta 1261:223-232(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT LYS-347 DEL.
      Tissue: Adrenal gland and Placenta.
    4. Maruyama K., Nishijima S., Kuromitsu S., Ichikawa A., Masuda E., Takemoto T., Kodama H., Kawashima H.
      Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    5. "Structural characterization and alternate splicing of the gene encoding the preadipocyte EGF-like protein pref-1."
      Smas C.M., Green D., Sul H.S.
      Biochemistry 33:9257-9265(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-8, ALTERNATIVE SPLICING.
    6. "Glycosylation analysis and protein structure determination of murine fetal antigen 1 (mFA1) -- the circulating gene product of the delta-like protein (dlk), preadipocyte factor 1 (Pref-1) and stromal-cell-derived protein 1 (SCP-1) cDNAs."
      Krogh T.N., Bachmann E., Teisner B., Skjoedt K., Hoejrup P.
      Eur. J. Biochem. 244:334-342(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION AT SER-94; ASN-100; ASN-165; ASN-174; SER-216; THR-224; THR-258; THR-267 AND THR-271, STRUCTURE OF CARBOHYDRATE.
    7. Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiDLK1_MOUSE
    AccessioniPrimary (citable) accession number: Q09163
    Secondary accession number(s): Q07645, Q62208
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3