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Protein

Protein delta homolog 1

Gene

Dlk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation.2 Publications

GO - Molecular functioni

GO - Biological processi

  • embryonic skeletal system development Source: MGI
  • negative regulation of fat cell differentiation Source: HGNC
  • negative regulation of Notch signaling pathway Source: MGI
  • Notch signaling pathway Source: Reactome
  • post-embryonic development Source: MGI
  • regulation of gene expression Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein delta homolog 1
Short name:
DLK-1
Alternative name(s):
Adipocyte differentiation inhibitor protein
Preadipocyte factor 1
Short name:
Pref-1
Cleaved into the following chain:
Fetal antigen 1
Short name:
FA1
Gene namesi
Name:Dlk1
Synonyms:Dlk, Pref1, Scp-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:94900. Dlk1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 305ExtracellularSequence analysisAdd BLAST282
Transmembranei306 – 329HelicalSequence analysisAdd BLAST24
Topological domaini330 – 385CytoplasmicSequence analysisAdd BLAST56

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23By similarityAdd BLAST23
ChainiPRO_000000752024 – 385Protein delta homolog 1Add BLAST362
ChainiPRO_000000752124 – 305Fetal antigen 1Add BLAST282

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 37PROSITE-ProRule annotation
Disulfide bondi30 ↔ 43PROSITE-ProRule annotation
Disulfide bondi45 ↔ 54PROSITE-ProRule annotation
Disulfide bondi57 ↔ 68PROSITE-ProRule annotation
Disulfide bondi63 ↔ 74PROSITE-ProRule annotation
Disulfide bondi76 ↔ 85PROSITE-ProRule annotation
Disulfide bondi92 ↔ 103PROSITE-ProRule annotation
GlycosylationiCAR_00016094O-linked (GalNAc...)1 Publication1
Disulfide bondi97 ↔ 113PROSITE-ProRule annotation
GlycosylationiCAR_000183100N-linked (GlcNAc...)1 Publication1
Disulfide bondi115 ↔ 124PROSITE-ProRule annotation
Disulfide bondi131 ↔ 144PROSITE-ProRule annotation
Disulfide bondi138 ↔ 156PROSITE-ProRule annotation
Disulfide bondi158 ↔ 167PROSITE-ProRule annotation
Glycosylationi165N-linked (GlcNAc...); atypical; partial1 Publication1
Glycosylationi174N-linked (GlcNAc...); atypical1 Publication1
Disulfide bondi176 ↔ 187PROSITE-ProRule annotation
Disulfide bondi181 ↔ 196PROSITE-ProRule annotation
Disulfide bondi198 ↔ 207PROSITE-ProRule annotation
Disulfide bondi214 ↔ 225PROSITE-ProRule annotation
GlycosylationiCAR_000161216O-linked (GalNAc...)1 Publication1
Disulfide bondi219 ↔ 235PROSITE-ProRule annotation
GlycosylationiCAR_000162224O-linked (GalNAc...)1 Publication1
Disulfide bondi237 ↔ 246PROSITE-ProRule annotation
Glycosylationi258O-linked (GalNAc...)1 Publication1
Glycosylationi267O-linked (GalNAc...); partial1 Publication1
Glycosylationi271O-linked (GalNAc...)1 Publication1
Glycosylationi295N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N- and O-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ09163.
PRIDEiQ09163.

PTM databases

iPTMnetiQ09163.
PhosphoSitePlusiQ09163.
UniCarbKBiQ09163.

Expressioni

Tissue specificityi

Highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary and, to a lesser degree, in adult kidney, muscle, thymus and heart.1 Publication

Developmental stagei

Expression is elevated in liver after birth but starts to decline around postnatal day 16.1 Publication

Gene expression databases

CleanExiMM_DLK1.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

DIPiDIP-6010N.
IntActiQ09163. 1 interactor.
STRINGi10090.ENSMUSP00000063104.

Structurei

3D structure databases

ProteinModelPortaliQ09163.
SMRiQ09163.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 55EGF-like 1PROSITE-ProRule annotationAdd BLAST32
Domaini53 – 86EGF-like 2PROSITE-ProRule annotationAdd BLAST34
Domaini88 – 125EGF-like 3PROSITE-ProRule annotationAdd BLAST38
Domaini127 – 168EGF-like 4PROSITE-ProRule annotationAdd BLAST42
Domaini172 – 208EGF-like 5PROSITE-ProRule annotationAdd BLAST37
Domaini210 – 247EGF-like 6PROSITE-ProRule annotationAdd BLAST38

Sequence similaritiesi

Contains 6 EGF-like domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1217. Eukaryota.
ENOG410XP6K. LUCA.
HOGENOMiHOG000072581.
HOVERGENiHBG007065.
InParanoidiQ09163.
PhylomeDBiQ09163.

Family and domain databases

InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
[Graphical view]
PfamiPF00008. EGF. 4 hits.
[Graphical view]
SMARTiSM00181. EGF. 6 hits.
SM00179. EGF_CA. 4 hits.
[Graphical view]
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS00022. EGF_1. 5 hits.
PS01186. EGF_2. 6 hits.
PS50026. EGF_3. 6 hits.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform A (identifier: Q09163-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIATGALLRV LLLLLAFGHS TYGAECDPPC DPQYGFCEAD NVCRCHVGWE
60 70 80 90 100
GPLCDKCVTA PGCVNGVCKE PWQCICKDGW DGKFCEIDVR ACTSTPCANN
110 120 130 140 150
GTCVDLEKGQ YECSCTPGFS GKDCQHKAGP CVINGSPCQH GGACVDDEGQ
160 170 180 190 200
ASHASCLCPP GFSGNFCEIV AATNSCTPNP CENDGVCTDI GGDFRCRCPA
210 220 230 240 250
GFVDKTCSRP VSNCASGPCQ NGGTCLQHTQ VSFECLCKPP FMGPTCAKKR
260 270 280 290 300
GASPVQVTHL PSGYGLTYRL TPGVHELPVQ QPEQHILKVS MKELNKSTPL
310 320 330 340 350
LTEGQAICFT ILGVLTSLVV LGTVAIVFLN KCETWVSNLR YNHTFRKKKN
360 370 380
LLLQYNSGEE LAVNIIFPEK IDMTTFNKEA GDEEI
Length:385
Mass (Da):41,320
Last modified:November 1, 1995 - v1
Checksum:iE79864FEA5AF4FF1
GO
Isoform B (identifier: Q09163-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     231-281: Missing.

Show »
Length:334
Mass (Da):35,811
Checksum:iCAEDBE46F3361527
GO
Isoform C (identifier: Q09163-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     231-303: Missing.

Show »
Length:312
Mass (Da):33,293
Checksum:i9201205F68663CC4
GO
Isoform C2 (identifier: Q09163-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     231-305: Missing.

Show »
Length:310
Mass (Da):33,108
Checksum:i0BDD15CDC082E4AD
GO
Isoform D (identifier: Q09163-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     211-303: Missing.

Show »
Length:292
Mass (Da):31,306
Checksum:i25CC1D603930C4EC
GO
Isoform D2 (identifier: Q09163-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     211-305: Missing.

Show »
Length:290
Mass (Da):31,121
Checksum:iE6E2449B38AA267E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti250R → P in AAA37175 (PubMed:8500166).Curated1
Sequence conflicti320 – 385VLGTV…GDEEI → CWAPWPSSFSTSAKPGCPTC ATTTCFARRRTSCCSITAAR SWRSISSSPRRLT in AAA37175 (PubMed:8500166).CuratedAdd BLAST66
Sequence conflicti344 – 345TF → ML in BAA04121 (Ref. 4) Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti347Missing .1 Publication1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001379211 – 305Missing in isoform D2. CuratedAdd BLAST95
Alternative sequenceiVSP_001378211 – 303Missing in isoform D. CuratedAdd BLAST93
Alternative sequenceiVSP_001382231 – 305Missing in isoform C2. CuratedAdd BLAST75
Alternative sequenceiVSP_001381231 – 303Missing in isoform C. CuratedAdd BLAST73
Alternative sequenceiVSP_001380231 – 281Missing in isoform B. CuratedAdd BLAST51

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12171 mRNA. Translation: CAA78162.1.
U15980 mRNA. Translation: AAB60495.1.
L12721 mRNA. Translation: AAA37175.1.
S71340 Genomic DNA. No translation available.
D16847 mRNA. Translation: BAA04121.1.
CCDSiCCDS26168.1. [Q09163-1]
CCDS49173.1. [Q09163-6]
CCDS49174.1. [Q09163-2]
CCDS56863.1. [Q09163-3]
PIRiA54785.
S53718.
RefSeqiNP_034182.2. NM_010052.5.
UniGeneiMm.157069.

Genome annotation databases

GeneIDi13386.
KEGGimmu:13386.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12171 mRNA. Translation: CAA78162.1.
U15980 mRNA. Translation: AAB60495.1.
L12721 mRNA. Translation: AAA37175.1.
S71340 Genomic DNA. No translation available.
D16847 mRNA. Translation: BAA04121.1.
CCDSiCCDS26168.1. [Q09163-1]
CCDS49173.1. [Q09163-6]
CCDS49174.1. [Q09163-2]
CCDS56863.1. [Q09163-3]
PIRiA54785.
S53718.
RefSeqiNP_034182.2. NM_010052.5.
UniGeneiMm.157069.

3D structure databases

ProteinModelPortaliQ09163.
SMRiQ09163.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-6010N.
IntActiQ09163. 1 interactor.
STRINGi10090.ENSMUSP00000063104.

PTM databases

iPTMnetiQ09163.
PhosphoSitePlusiQ09163.
UniCarbKBiQ09163.

Proteomic databases

PaxDbiQ09163.
PRIDEiQ09163.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi13386.
KEGGimmu:13386.

Organism-specific databases

CTDi8788.
MGIiMGI:94900. Dlk1.

Phylogenomic databases

eggNOGiKOG1217. Eukaryota.
ENOG410XP6K. LUCA.
HOGENOMiHOG000072581.
HOVERGENiHBG007065.
InParanoidiQ09163.
PhylomeDBiQ09163.

Enzyme and pathway databases

ReactomeiR-MMU-2122948. Activated NOTCH1 Transmits Signal to the Nucleus.

Miscellaneous databases

PROiQ09163.
SOURCEiSearch...

Gene expression databases

CleanExiMM_DLK1.

Family and domain databases

InterProiIPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
[Graphical view]
PfamiPF00008. EGF. 4 hits.
[Graphical view]
SMARTiSM00181. EGF. 6 hits.
SM00179. EGF_CA. 4 hits.
[Graphical view]
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS00022. EGF_1. 5 hits.
PS01186. EGF_2. 6 hits.
PS50026. EGF_3. 6 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDLK1_MOUSE
AccessioniPrimary (citable) accession number: Q09163
Secondary accession number(s): Q07645, Q62208
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.