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Q09163

- DLK1_MOUSE

UniProt

Q09163 - DLK1_MOUSE

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Protein
Protein delta homolog 1
Gene
Dlk1, Dlk, Pref1, Scp-1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation.2 Publications

GO - Molecular functioni

  1. calcium ion binding Source: InterPro

GO - Biological processi

  1. embryonic skeletal system development Source: MGI
  2. negative regulation of Notch signaling pathway Source: MGI
  3. post-embryonic development Source: MGI
  4. regulation of gene expression Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_199384. Activated NOTCH1 Transmits Signal to the Nucleus.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein delta homolog 1
Short name:
DLK-1
Alternative name(s):
Adipocyte differentiation inhibitor protein
Preadipocyte factor 1
Short name:
Pref-1
Cleaved into the following chain:
Fetal antigen 1
Short name:
FA1
Gene namesi
Name:Dlk1
Synonyms:Dlk, Pref1, Scp-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:94900. Dlk1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 305282Extracellular Reviewed prediction
Add
BLAST
Transmembranei306 – 32924Helical; Reviewed prediction
Add
BLAST
Topological domaini330 – 38556Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. external side of plasma membrane Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
  3. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 By similarity
Add
BLAST
Chaini24 – 385362Protein delta homolog 1
PRO_0000007520Add
BLAST
Chaini24 – 305282Fetal antigen 1
PRO_0000007521Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi26 ↔ 37 By similarity
Disulfide bondi30 ↔ 43 By similarity
Disulfide bondi45 ↔ 54 By similarity
Disulfide bondi57 ↔ 68 By similarity
Disulfide bondi63 ↔ 74 By similarity
Disulfide bondi76 ↔ 85 By similarity
Disulfide bondi92 ↔ 103 By similarity
Glycosylationi94 – 941O-linked (GalNAc...)1 Publication
CAR_000160
Disulfide bondi97 ↔ 113 By similarity
Glycosylationi100 – 1001N-linked (GlcNAc...)1 Publication
CAR_000183
Disulfide bondi115 ↔ 124 By similarity
Disulfide bondi131 ↔ 144 By similarity
Disulfide bondi138 ↔ 156 By similarity
Disulfide bondi158 ↔ 167 By similarity
Glycosylationi165 – 1651N-linked (GlcNAc...); partial; atypical1 Publication
Glycosylationi174 – 1741N-linked (GlcNAc...); atypical1 Publication
Disulfide bondi176 ↔ 187 By similarity
Disulfide bondi181 ↔ 196 By similarity
Disulfide bondi198 ↔ 207 By similarity
Disulfide bondi214 ↔ 225 By similarity
Glycosylationi216 – 2161O-linked (GalNAc...)1 Publication
CAR_000161
Disulfide bondi219 ↔ 235 By similarity
Glycosylationi224 – 2241O-linked (GalNAc...)1 Publication
CAR_000162
Disulfide bondi237 ↔ 246 By similarity
Glycosylationi258 – 2581O-linked (GalNAc...)1 Publication
Glycosylationi267 – 2671O-linked (GalNAc...); partial1 Publication
Glycosylationi271 – 2711O-linked (GalNAc...)1 Publication
Glycosylationi295 – 2951N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

N- and O-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ09163.
PRIDEiQ09163.

PTM databases

PhosphoSiteiQ09163.
UniCarbKBiQ09163.

Expressioni

Tissue specificityi

Highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary and, to a lesser degree, in adult kidney, muscle, thymus and heart.1 Publication

Developmental stagei

Expression is elevated in liver after birth but starts to decline around postnatal day 16.1 Publication

Gene expression databases

CleanExiMM_DLK1.
GenevestigatoriQ09163.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

DIPiDIP-6010N.
IntActiQ09163. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ09163.
SMRiQ09163. Positions 17-288.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 5532EGF-like 1
Add
BLAST
Domaini53 – 8634EGF-like 2
Add
BLAST
Domaini88 – 12538EGF-like 3
Add
BLAST
Domaini127 – 16842EGF-like 4
Add
BLAST
Domaini172 – 20837EGF-like 5
Add
BLAST
Domaini210 – 24738EGF-like 6
Add
BLAST

Sequence similaritiesi

Contains 6 EGF-like domains.

Keywords - Domaini

EGF-like domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG306005.
HOGENOMiHOG000072581.
HOVERGENiHBG007065.
InParanoidiQ09163.
PhylomeDBiQ09163.

Family and domain databases

InterProiIPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
[Graphical view]
PfamiPF00008. EGF. 4 hits.
[Graphical view]
SMARTiSM00181. EGF. 5 hits.
SM00179. EGF_CA. 1 hit.
[Graphical view]
PROSITEiPS00010. ASX_HYDROXYL. 1 hit.
PS00022. EGF_1. 5 hits.
PS01186. EGF_2. 6 hits.
PS50026. EGF_3. 6 hits.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 6 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform A (identifier: Q09163-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MIATGALLRV LLLLLAFGHS TYGAECDPPC DPQYGFCEAD NVCRCHVGWE    50
GPLCDKCVTA PGCVNGVCKE PWQCICKDGW DGKFCEIDVR ACTSTPCANN 100
GTCVDLEKGQ YECSCTPGFS GKDCQHKAGP CVINGSPCQH GGACVDDEGQ 150
ASHASCLCPP GFSGNFCEIV AATNSCTPNP CENDGVCTDI GGDFRCRCPA 200
GFVDKTCSRP VSNCASGPCQ NGGTCLQHTQ VSFECLCKPP FMGPTCAKKR 250
GASPVQVTHL PSGYGLTYRL TPGVHELPVQ QPEQHILKVS MKELNKSTPL 300
LTEGQAICFT ILGVLTSLVV LGTVAIVFLN KCETWVSNLR YNHTFRKKKN 350
LLLQYNSGEE LAVNIIFPEK IDMTTFNKEA GDEEI 385
Length:385
Mass (Da):41,320
Last modified:November 1, 1995 - v1
Checksum:iE79864FEA5AF4FF1
GO
Isoform B (identifier: Q09163-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     231-281: Missing.

Show »
Length:334
Mass (Da):35,811
Checksum:iCAEDBE46F3361527
GO
Isoform C (identifier: Q09163-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     231-303: Missing.

Show »
Length:312
Mass (Da):33,293
Checksum:i9201205F68663CC4
GO
Isoform C2 (identifier: Q09163-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     231-305: Missing.

Show »
Length:310
Mass (Da):33,108
Checksum:i0BDD15CDC082E4AD
GO
Isoform D (identifier: Q09163-5) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     211-303: Missing.

Show »
Length:292
Mass (Da):31,306
Checksum:i25CC1D603930C4EC
GO
Isoform D2 (identifier: Q09163-6) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     211-305: Missing.

Show »
Length:290
Mass (Da):31,121
Checksum:iE6E2449B38AA267E
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti347 – 3471Missing.1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei211 – 30595Missing in isoform D2.
VSP_001379Add
BLAST
Alternative sequencei211 – 30393Missing in isoform D.
VSP_001378Add
BLAST
Alternative sequencei231 – 30575Missing in isoform C2.
VSP_001382Add
BLAST
Alternative sequencei231 – 30373Missing in isoform C.
VSP_001381Add
BLAST
Alternative sequencei231 – 28151Missing in isoform B.
VSP_001380Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti250 – 2501R → P in AAA37175. 1 Publication
Sequence conflicti320 – 38566VLGTV…GDEEI → CWAPWPSSFSTSAKPGCPTC ATTTCFARRRTSCCSITAAR SWRSISSSPRRLT in AAA37175. 1 Publication
Add
BLAST
Sequence conflicti344 – 3452TF → ML in BAA04121. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z12171 mRNA. Translation: CAA78162.1.
U15980 mRNA. Translation: AAB60495.1.
L12721 mRNA. Translation: AAA37175.1.
S71340 Genomic DNA. No translation available.
D16847 mRNA. Translation: BAA04121.1.
CCDSiCCDS26168.1. [Q09163-1]
CCDS49173.1. [Q09163-6]
CCDS49174.1. [Q09163-2]
CCDS56863.1. [Q09163-3]
PIRiA54785.
S53718.
RefSeqiNP_034182.2. NM_010052.5.
UniGeneiMm.157069.

Genome annotation databases

GeneIDi13386.
KEGGimmu:13386.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z12171 mRNA. Translation: CAA78162.1 .
U15980 mRNA. Translation: AAB60495.1 .
L12721 mRNA. Translation: AAA37175.1 .
S71340 Genomic DNA. No translation available.
D16847 mRNA. Translation: BAA04121.1 .
CCDSi CCDS26168.1. [Q09163-1 ]
CCDS49173.1. [Q09163-6 ]
CCDS49174.1. [Q09163-2 ]
CCDS56863.1. [Q09163-3 ]
PIRi A54785.
S53718.
RefSeqi NP_034182.2. NM_010052.5.
UniGenei Mm.157069.

3D structure databases

ProteinModelPortali Q09163.
SMRi Q09163. Positions 17-288.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-6010N.
IntActi Q09163. 1 interaction.

PTM databases

PhosphoSitei Q09163.
UniCarbKBi Q09163.

Proteomic databases

PaxDbi Q09163.
PRIDEi Q09163.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 13386.
KEGGi mmu:13386.

Organism-specific databases

CTDi 8788.
MGIi MGI:94900. Dlk1.

Phylogenomic databases

eggNOGi NOG306005.
HOGENOMi HOG000072581.
HOVERGENi HBG007065.
InParanoidi Q09163.
PhylomeDBi Q09163.

Enzyme and pathway databases

Reactomei REACT_199384. Activated NOTCH1 Transmits Signal to the Nucleus.

Miscellaneous databases

ChiTaRSi DLK1. mouse.
NextBioi 283744.
PROi Q09163.
SOURCEi Search...

Gene expression databases

CleanExi MM_DLK1.
Genevestigatori Q09163.

Family and domain databases

InterProi IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
[Graphical view ]
Pfami PF00008. EGF. 4 hits.
[Graphical view ]
SMARTi SM00181. EGF. 5 hits.
SM00179. EGF_CA. 1 hit.
[Graphical view ]
PROSITEi PS00010. ASX_HYDROXYL. 1 hit.
PS00022. EGF_1. 5 hits.
PS01186. EGF_2. 6 hits.
PS50026. EGF_3. 6 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "dlk, a putative mammalian homeotic gene differentially expressed in small cell lung carcinoma and neuroendocrine tumor cell line."
    Laborda J., Sausville E.A., Hoffman T., Notario V.
    J. Biol. Chem. 268:3817-3820(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: Swiss.
    Tissue: Fibroblast.
  2. "Pref-1, a protein containing EGF-like repeats, inhibits adipocyte differentiation."
    Smas C.M., Sul H.S.
    Cell 73:725-734(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  3. "dlk, pG2 and Pref-1 mRNAs encode similar proteins belonging to the EGF-like superfamily. Identification of polymorphic variants of this RNA."
    Lee Y.L., Helman L., Hoffman T., Laborda J.
    Biochim. Biophys. Acta 1261:223-232(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT LYS-347 DEL.
    Tissue: Adrenal gland and Placenta.
  4. Maruyama K., Nishijima S., Kuromitsu S., Ichikawa A., Masuda E., Takemoto T., Kodama H., Kawashima H.
    Submitted (AUG-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "Structural characterization and alternate splicing of the gene encoding the preadipocyte EGF-like protein pref-1."
    Smas C.M., Green D., Sul H.S.
    Biochemistry 33:9257-9265(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-8, ALTERNATIVE SPLICING.
  6. "Glycosylation analysis and protein structure determination of murine fetal antigen 1 (mFA1) -- the circulating gene product of the delta-like protein (dlk), preadipocyte factor 1 (Pref-1) and stromal-cell-derived protein 1 (SCP-1) cDNAs."
    Krogh T.N., Bachmann E., Teisner B., Skjoedt K., Hoejrup P.
    Eur. J. Biochem. 244:334-342(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION AT SER-94; ASN-100; ASN-165; ASN-174; SER-216; THR-224; THR-258; THR-267 AND THR-271, STRUCTURE OF CARBOHYDRATE.
  7. Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiDLK1_MOUSE
AccessioniPrimary (citable) accession number: Q09163
Secondary accession number(s): Q07645, Q62208
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 3, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi