Q09140 (UGGG_SCHPO) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 93.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: UDP-glucose:glycoprotein glucosyltransferase EC=2.4.1.- Alternative name(s): UDP--Glc:glycoprotein glucosyltransferase Short name=UGT | ||||
| Gene names |
| ||||
| Organism | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) | ||||
| Taxonomic identifier | 284812 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces |
Protein attributes
| Sequence length | 1448 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Selectively reglucosylates unfolded glycoproteins, thus providing quality control for protein transport out of the ER. Unfolded, denatured glycoproteins are substantially better substrates for glucosylation by this enzyme than are the corresponding native proteins. This protein and transient glucosylation may be involved in monitoring and/or assisting the folding and assembly of newly made glycoproteins, in order to identify glycoproteins that need assistance in folding from chaperones. Ref.1 |
| Cofactor | Calcium or manganese. Ref.1 |
| Pathway | |
| Subunit structure | Monomer. Ref.1 |
| Subcellular location | |
| Post-translational modification | Glycosylated. Ref.1 |
| Sequence similarities | Belongs to the glycosyltransferase 8 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Endoplasmic reticulum |
| Domain | Signal |
| Molecular function | Glycosyltransferase Transferase |
| PTM | Glycoprotein |
| Technical term | Complete proteome Direct protein sequencing Reference proteome |
| Gene Ontology (GO) | |
| Biological process | (1->6)-beta-D-glucan biosynthetic process Inferred from sequence or structural similarity. Source: GeneDB_Spombe cellular response to stressInferred from mutant phenotype. Source: GeneDB_Spombe protein glycosylationInferred from direct assay. Source: GeneDB_Spombe |
| Cellular component | endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | UDP-glucose:glycoprotein glucosyltransferase activity Inferred from mutant phenotype. Source: GeneDB_Spombe |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 18 | 18 | Ref.1 Ref.3 | ||||||
| Chain | 19 – 1448 | 1430 | UDP-glucose:glycoprotein glucosyltransferase | PRO_0000012275 | |||||
Regions | |||||||||
| Region | 1149 – 1448 | 300 | Glucosyltransferase By similarity | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 66 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 749 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 880 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 1046 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 1441 | 1 | N-linked (GlcNAc...) Potential | ||||||
Experimental info | |||||||||
| Sequence conflict | 118 | 1 | S → T in AAB05993. Ref.1 | ||||||
| Sequence conflict | 291 | 1 | T → A in AAB05993. Ref.1 | ||||||
| Sequence conflict | 295 | 1 | T → S in AAB05993. Ref.1 | ||||||
| Sequence conflict | 320 | 1 | D → H in AAB05993. Ref.1 | ||||||
| Sequence conflict | 928 | 1 | F → L in AAB05993. Ref.1 | ||||||
| Sequence conflict | 1199 | 1 | S → C in AAB05993. Ref.1 | ||||||
| Sequence conflict | 1261 | 1 | Missing in AAB05993. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "A new stress protein: synthesis of Schizosaccharomyces pombe UDP--Glc:glycoprotein glucosyltransferase mRNA is induced by stress conditions but the enzyme is not essential for cell viability." Fernandez F., Jannatipour M., Hellman U., Rokeach L.A., Parodi A.J. EMBO J. 15:705-713(1996) [PubMed: 8631292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 19-31; 148-158; 401-412; 557-568; 723-730; 983-990 AND 1118-1130, FUNCTION, SUBCELLULAR LOCATION, COFACTOR, SUBUNIT, GLYCOSYLATION. |
| [2] | "The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. Nurse P.Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843. |
| [3] | "Purification to homogeneity of UDP-glucose:glycoprotein glucosyltransferase from Schizosaccharomyces pombe and apparent absence of the enzyme from Saccharomyces cerevisiae." Fernandez F.S., Trombetta S.E., Hellman U., Parodi A.J. J. Biol. Chem. 269:30701-30706(1994) [PubMed: 7982990] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-31, CHARACTERIZATION. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U38417 Genomic DNA. Translation: AAB05993.1. CU329671 Genomic DNA. Translation: CAC38351.1. |
| PIR | S63669. |
| RefSeq | NP_595281.1. NM_001021188.1. |
3D structure databases | |
| ProteinModelPortal | Q09140. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GT24. Glycosyltransferase Family 24. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblFungi | SPBPJ4664.06.1; SPBPJ4664.06.1:pep; SPBPJ4664.06. |
| GeneID | 2541413. |
| GenomeReviews | Gene locus gpt1 in contig CU329671_GR. |
| KEGG | spo:SPBPJ4664.06. |
| NMPDR | fig|4896.1.peg.1147. |
Organism-specific databases | |
| GeneDB_Spombe | SPBPJ4664.06. |
Phylogenomic databases | |
| eggNOG | fuNOG07258. |
| GeneTree | EFGT00050000006372. |
| HOGENOM | HBG396631. |
| OMA | IEGHARE. |
| OrthoDB | EOG479JG7. |
Enzyme and pathway databases | |
| BioCyc | SPOM-XXX-01:SPOM-XXX-01-003364-MONOMER. |
Gene expression databases | |
| ArrayExpress | Q09140. |
Family and domain databases | |
| InterPro | IPR002495. Glyco_trans_8. IPR009448. UDP-g_GGtrans. [Graphical view] |
| KO | K11718. |
| PANTHER | PTHR11226. UDP-g_GGtrans. 1 hit. |
| Pfam | PF01501. Glyco_transf_8. 1 hit. PF06427. UDP-g_GGTase. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | UGGG_SCHPO | ||||||||
| Accession | Primary (citable) accession number: Q09140 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Fungal Protein Annotation Program | ||||||||
Relevant documents
| Schizosaccharomyces pombe Schizosaccharomyces pombe: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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