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Protein

Purple acid phosphatase

Gene

PAP

Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.2 Publications

Cofactori

Protein has several cofactor binding sites:

Absorptioni

Abs(max)=550 nm1 Publication

Kineticsi

  1. KM=8 µM for p-NPP (at pH 5.5 and 25 degrees Celsius)1 Publication
  2. KM=5 µM for ATP (at pH 5.5 and 25 degrees Celsius)1 Publication
  3. KM=6 µM for ADP (at pH 5.5 and 25 degrees Celsius)1 Publication
  4. KM=9 µM for AMP (at pH 5.5 and 25 degrees Celsius)1 Publication
  5. KM=9 µM for pyrophosphate (at pH 5.5 and 25 degrees Celsius)1 Publication
  6. KM=30 µM for beta-glycerophosphate (at pH 5.5 and 25 degrees Celsius)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi162 – 1621IronBy similarity
Metal bindingi191 – 1911IronBy similarity
Metal bindingi191 – 1911ZincBy similarity
Metal bindingi194 – 1941IronBy similarity
Metal bindingi228 – 2281ZincBy similarity
Binding sitei228 – 2281SubstrateBy similarity
Metal bindingi313 – 3131ZincBy similarity
Active sitei323 – 3231Proton donorBy similarity
Metal bindingi350 – 3501ZincBy similarity
Metal bindingi352 – 3521IronBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB
  2. manganese ion binding Source: UniProtKB
  3. metal ion binding Source: UniProtKB

GO - Biological processi

  1. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15155.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase (EC:3.1.3.2)
Alternative name(s):
Zinc(II) purple acid phosphatase
Gene namesi
Name:PAPImported
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
ProteomesiUP000008827 Componenti: Unplaced

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 30301 PublicationAdd
BLAST
Chaini31 – 464434Purple acid phosphatase1 PublicationPRO_0000043380Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence Analysis
Glycosylationi136 – 1361N-linked (GlcNAc...)Sequence Analysis
Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence Analysis
Glycosylationi301 – 3011N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi372 – 372InterchainBy similarity
Glycosylationi398 – 3981N-linked (GlcNAc...)Sequence Analysis
Glycosylationi423 – 4231N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Gene expression databases

GenevestigatoriQ09131.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ09131.
SMRiQ09131. Positions 37-459.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni350 – 3523Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiQ09131.

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q09131-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVVEGLLAL ALVLSACVMC NGGSSSPFIR KVEKTVDMPL DSDVFAVPPG
60 70 80 90 100
YNAPQQVHIT QGDLVGKAVI VSWVTVDEPG SSEVHYWSEN SDKKKIAEGK
110 120 130 140 150
LVTYRFFNYS SGFIHHTTIR NLEYKTKYYY EVGLGNTTRQ FWFVTPPEIG
160 170 180 190 200
PDVPYTFGLI GDLGQSFDSN KTLSHYELNP RKGQTVLFVG DLSYADNYPN
210 220 230 240 250
HDNIRWDSWG RFTERSVAYQ PWIWTAGNHE NHFAPEIGET VPFKPYTHRY
260 270 280 290 300
HVPYKASQST SPFWYSIKRA SAHIIVLASY SAYGKYTPQY KWLEKELPKV
310 320 330 340 350
NRTETPWLIV LMHSPWYNSY NYHYMEGETM RVMYEPWFVQ YKVDVVFAGH
360 370 380 390 400
VHAYERSERV SNVAYNIVNG LCAPVNDKSA PVYITIGDGG TLEGLATNMT
410 420 430 440 450
EPQPKYSAFR EASFGHAIFD ITNRTHAHYS WHRNQDGVAV EADSLWSFNR
460
YWHPVDDSTA HVSH
Length:464
Mass (Da):53,027
Last modified:December 5, 2005 - v2
Checksum:i83BBAD593BEB01F5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 351T → A AA sequence (PubMed:16668896).Curated
Sequence conflicti47 – 471V → R AA sequence (PubMed:16668896).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF200824 mRNA. Translation: AAF19820.1.
PIRiB59200.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF200824 mRNA. Translation: AAF19820.1.
PIRiB59200.

3D structure databases

ProteinModelPortaliQ09131.
SMRiQ09131. Positions 37-459.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

InParanoidiQ09131.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15155.

Gene expression databases

GenevestigatoriQ09131.

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Binuclear metal centers in plant purple acid phosphatases: Fe-Mn in sweet potato and Fe-Zn in soybean."
    Schenk G., Ge Y., Carrington L.E., Wynne C.J., Searle I.R., Carroll B.J., Hamilton S., de Jersey J.
    Arch. Biochem. Biophys. 370:183-189(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: cv. Provar.
  2. "Purification and characterization of a secreted purple phosphatase from soybean suspension cultures."
    Lebansky B.R., McKnight T.D., Griffing L.R.
    Plant Physiol. 99:391-395(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 31-47, CATALYTIC ACTIVITY, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPPAF_SOYBN
AccessioniPrimary (citable) accession number: Q09131
Secondary accession number(s): Q9SE01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2005
Last sequence update: December 5, 2005
Last modified: January 6, 2015
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.