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Q09131

- PPAF_SOYBN

UniProt

Q09131 - PPAF_SOYBN

Protein

Purple acid phosphatase

Gene

PAP

Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 2 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.2 Publications

    Cofactori

    Binds 1 iron ion per subunit.1 Publication
    Binds 1 zinc ion per subunit. Can also use manganese, copper and magnesium ions.

    Absorptioni

    Abs(max)=550 nm1 Publication

    Kineticsi

    1. KM=8 µM for p-NPP (at pH 5.5 and 25 degrees Celsius)1 Publication
    2. KM=5 µM for ATP (at pH 5.5 and 25 degrees Celsius)1 Publication
    3. KM=6 µM for ADP (at pH 5.5 and 25 degrees Celsius)1 Publication
    4. KM=9 µM for AMP (at pH 5.5 and 25 degrees Celsius)1 Publication
    5. KM=9 µM for pyrophosphate (at pH 5.5 and 25 degrees Celsius)1 Publication
    6. KM=30 µM for beta-glycerophosphate (at pH 5.5 and 25 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi162 – 1621IronBy similarity
    Metal bindingi191 – 1911IronBy similarity
    Metal bindingi191 – 1911ZincBy similarity
    Metal bindingi194 – 1941IronBy similarity
    Metal bindingi228 – 2281ZincBy similarity
    Binding sitei228 – 2281SubstrateBy similarity
    Metal bindingi313 – 3131ZincBy similarity
    Active sitei323 – 3231Proton donorBy similarity
    Metal bindingi350 – 3501ZincBy similarity
    Metal bindingi352 – 3521IronBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: UniProtKB
    2. manganese ion binding Source: UniProtKB
    3. metal ion binding Source: UniProtKB

    GO - Biological processi

    1. dephosphorylation Source: GOC

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Iron, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15155.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Purple acid phosphatase (EC:3.1.3.2)
    Alternative name(s):
    Zinc(II) purple acid phosphatase
    Gene namesi
    Name:PAPImported
    OrganismiGlycine max (Soybean) (Glycine hispida)
    Taxonomic identifieri3847 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
    ProteomesiUP000008827: Unplaced

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 30301 PublicationAdd
    BLAST
    Chaini31 – 464434Purple acid phosphatase1 PublicationPRO_0000043380Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi108 – 1081N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi136 – 1361N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi301 – 3011N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi372 – 372InterchainBy similarity
    Glycosylationi398 – 3981N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi423 – 4231N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Gene expression databases

    GenevestigatoriQ09131.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.By similarity1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ09131.
    SMRiQ09131. Positions 37-459.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni350 – 3523Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.40.380. 1 hit.
    3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR008963. Purple_acid_Pase-like_N.
    IPR015914. Purple_acid_Pase_N.
    IPR025733. Purple_acid_PPase_C_dom.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    PF14008. Metallophos_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF49363. SSF49363. 1 hit.
    SSF56300. SSF56300. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q09131-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGVVEGLLAL ALVLSACVMC NGGSSSPFIR KVEKTVDMPL DSDVFAVPPG    50
    YNAPQQVHIT QGDLVGKAVI VSWVTVDEPG SSEVHYWSEN SDKKKIAEGK 100
    LVTYRFFNYS SGFIHHTTIR NLEYKTKYYY EVGLGNTTRQ FWFVTPPEIG 150
    PDVPYTFGLI GDLGQSFDSN KTLSHYELNP RKGQTVLFVG DLSYADNYPN 200
    HDNIRWDSWG RFTERSVAYQ PWIWTAGNHE NHFAPEIGET VPFKPYTHRY 250
    HVPYKASQST SPFWYSIKRA SAHIIVLASY SAYGKYTPQY KWLEKELPKV 300
    NRTETPWLIV LMHSPWYNSY NYHYMEGETM RVMYEPWFVQ YKVDVVFAGH 350
    VHAYERSERV SNVAYNIVNG LCAPVNDKSA PVYITIGDGG TLEGLATNMT 400
    EPQPKYSAFR EASFGHAIFD ITNRTHAHYS WHRNQDGVAV EADSLWSFNR 450
    YWHPVDDSTA HVSH 464
    Length:464
    Mass (Da):53,027
    Last modified:December 6, 2005 - v2
    Checksum:i83BBAD593BEB01F5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti35 – 351T → A AA sequence (PubMed:16668896)Curated
    Sequence conflicti47 – 471V → R AA sequence (PubMed:16668896)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF200824 mRNA. Translation: AAF19820.1.
    PIRiB59200.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF200824 mRNA. Translation: AAF19820.1 .
    PIRi B59200.

    3D structure databases

    ProteinModelPortali Q09131.
    SMRi Q09131. Positions 37-459.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-15155.

    Gene expression databases

    Genevestigatori Q09131.

    Family and domain databases

    Gene3Di 2.60.40.380. 1 hit.
    3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR008963. Purple_acid_Pase-like_N.
    IPR015914. Purple_acid_Pase_N.
    IPR025733. Purple_acid_PPase_C_dom.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    PF14008. Metallophos_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49363. SSF49363. 1 hit.
    SSF56300. SSF56300. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Binuclear metal centers in plant purple acid phosphatases: Fe-Mn in sweet potato and Fe-Zn in soybean."
      Schenk G., Ge Y., Carrington L.E., Wynne C.J., Searle I.R., Carroll B.J., Hamilton S., de Jersey J.
      Arch. Biochem. Biophys. 370:183-189(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: cv. Provar.
    2. "Purification and characterization of a secreted purple phosphatase from soybean suspension cultures."
      Lebansky B.R., McKnight T.D., Griffing L.R.
      Plant Physiol. 99:391-395(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 31-47, CATALYTIC ACTIVITY, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiPPAF_SOYBN
    AccessioniPrimary (citable) accession number: Q09131
    Secondary accession number(s): Q9SE01
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 66 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3