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Protein

Purple acid phosphatase

Gene

PAP

Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.2 Publications

Cofactori

Protein has several cofactor binding sites:

Absorptioni

Abs(max)=550 nm1 Publication

Kineticsi

  1. KM=8 µM for p-NPP (at pH 5.5 and 25 degrees Celsius)1 Publication
  2. KM=5 µM for ATP (at pH 5.5 and 25 degrees Celsius)1 Publication
  3. KM=6 µM for ADP (at pH 5.5 and 25 degrees Celsius)1 Publication
  4. KM=9 µM for AMP (at pH 5.5 and 25 degrees Celsius)1 Publication
  5. KM=9 µM for pyrophosphate (at pH 5.5 and 25 degrees Celsius)1 Publication
  6. KM=30 µM for beta-glycerophosphate (at pH 5.5 and 25 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi162IronBy similarity1
    Metal bindingi191IronBy similarity1
    Metal bindingi191ZincBy similarity1
    Metal bindingi194IronBy similarity1
    Metal bindingi228ZincBy similarity1
    Binding sitei228SubstrateBy similarity1
    Metal bindingi313ZincBy similarity1
    Active sitei323Proton donorBy similarity1
    Metal bindingi350ZincBy similarity1
    Metal bindingi352IronBy similarity1

    GO - Molecular functioni

    • acid phosphatase activity Source: UniProtKB
    • manganese ion binding Source: UniProtKB
    • metal ion binding Source: UniProtKB

    Keywordsi

    Molecular functionHydrolase
    LigandIron, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15155.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Purple acid phosphatase (EC:3.1.3.2)
    Alternative name(s):
    Zinc(II) purple acid phosphatase
    Gene namesi
    Name:PAPImported
    OrganismiGlycine max (Soybean) (Glycine hispida)
    Taxonomic identifieri3847 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
    Proteomesi
    • UP000008827 Componenti: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    • extracellular space Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 301 PublicationAdd BLAST30
    ChainiPRO_000004338031 – 464Purple acid phosphatase1 PublicationAdd BLAST434

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi108N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi136N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi170N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi301N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi372InterchainBy similarity
    Glycosylationi398N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi423N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ09131.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.By similarity1 Publication

    Protein-protein interaction databases

    STRINGi3847.GLYMA12G01000.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ09131.
    SMRiQ09131.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni350 – 352Substrate bindingBy similarity3

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiKOG1378. Eukaryota.
    COG1409. LUCA.
    InParanoidiQ09131.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiView protein in InterPro
    IPR004843. Calcineurin-like_PHP_ApaH.
    IPR029052. Metallo-depent_PP-like.
    IPR008963. Purple_acid_Pase-like_N.
    IPR015914. Purple_acid_Pase_N.
    IPR025733. Purple_acid_PPase_C_dom.
    PfamiView protein in Pfam
    PF00149. Metallophos. 1 hit.
    PF14008. Metallophos_C. 1 hit.
    PF16656. Pur_ac_phosph_N. 1 hit.
    SUPFAMiSSF49363. SSF49363. 1 hit.
    SSF56300. SSF56300. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q09131-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGVVEGLLAL ALVLSACVMC NGGSSSPFIR KVEKTVDMPL DSDVFAVPPG
    60 70 80 90 100
    YNAPQQVHIT QGDLVGKAVI VSWVTVDEPG SSEVHYWSEN SDKKKIAEGK
    110 120 130 140 150
    LVTYRFFNYS SGFIHHTTIR NLEYKTKYYY EVGLGNTTRQ FWFVTPPEIG
    160 170 180 190 200
    PDVPYTFGLI GDLGQSFDSN KTLSHYELNP RKGQTVLFVG DLSYADNYPN
    210 220 230 240 250
    HDNIRWDSWG RFTERSVAYQ PWIWTAGNHE NHFAPEIGET VPFKPYTHRY
    260 270 280 290 300
    HVPYKASQST SPFWYSIKRA SAHIIVLASY SAYGKYTPQY KWLEKELPKV
    310 320 330 340 350
    NRTETPWLIV LMHSPWYNSY NYHYMEGETM RVMYEPWFVQ YKVDVVFAGH
    360 370 380 390 400
    VHAYERSERV SNVAYNIVNG LCAPVNDKSA PVYITIGDGG TLEGLATNMT
    410 420 430 440 450
    EPQPKYSAFR EASFGHAIFD ITNRTHAHYS WHRNQDGVAV EADSLWSFNR
    460
    YWHPVDDSTA HVSH
    Length:464
    Mass (Da):53,027
    Last modified:December 6, 2005 - v2
    Checksum:i83BBAD593BEB01F5
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti35T → A AA sequence (PubMed:16668896).Curated1
    Sequence conflicti47V → R AA sequence (PubMed:16668896).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF200824 mRNA. Translation: AAF19820.1.
    PIRiB59200.
    UniGeneiGma.57276.
    Gma.58071.

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

    Entry informationi

    Entry nameiPPAF_SOYBN
    AccessioniPrimary (citable) accession number: Q09131
    Secondary accession number(s): Q9SE01
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: December 6, 2005
    Last modified: May 10, 2017
    This is version 79 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families