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Q09131

- PPAF_SOYBN

UniProt

Q09131 - PPAF_SOYBN

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Protein

Purple acid phosphatase

Gene
PAP
Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.2 Publications

Cofactori

Binds 1 iron ion per subunit.2 Publications
Binds 1 zinc ion per subunit. Can also use manganese, copper and magnesium ions.2 Publications

Absorptioni

Abs(max)=550 nm1 Publication

Kineticsi

  1. KM=8 µM for p-NPP (at pH 5.5 and 25 degrees Celsius)
  2. KM=5 µM for ATP (at pH 5.5 and 25 degrees Celsius)
  3. KM=6 µM for ADP (at pH 5.5 and 25 degrees Celsius)
  4. KM=9 µM for AMP (at pH 5.5 and 25 degrees Celsius)
  5. KM=9 µM for pyrophosphate (at pH 5.5 and 25 degrees Celsius)
  6. KM=30 µM for beta-glycerophosphate (at pH 5.5 and 25 degrees Celsius)

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi162 – 1621Iron By similarityBy similarity
Metal bindingi191 – 1911Iron By similarityBy similarity
Metal bindingi191 – 1911Zinc By similarityBy similarity
Metal bindingi194 – 1941Iron By similarityBy similarity
Metal bindingi228 – 2281Zinc By similarityBy similarity
Binding sitei228 – 2281Substrate By similarity
Metal bindingi313 – 3131Zinc By similarityBy similarity
Active sitei323 – 3231Proton donor By similarityBy similarity
Metal bindingi350 – 3501Zinc By similarityBy similarity
Metal bindingi352 – 3521Iron By similarityBy similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB
  2. manganese ion binding Source: UniProtKB
  3. metal ion binding Source: UniProtKB

GO - Biological processi

  1. dephosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15155.

Names & Taxonomyi

Protein namesi
Recommended name:
Purple acid phosphatase (EC:3.1.3.2)
Alternative name(s):
Zinc(II) purple acid phosphatase
Gene namesi
Name:PAP
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
ProteomesiUP000008827: Unplaced

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 30302 PublicationsAdd
BLAST
Chaini31 – 464434Purple acid phosphatase1 PublicationPRO_0000043380Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi108 – 1081N-linked (GlcNAc...) Reviewed prediction
Glycosylationi136 – 1361N-linked (GlcNAc...) Reviewed prediction
Glycosylationi170 – 1701N-linked (GlcNAc...) Reviewed prediction
Glycosylationi301 – 3011N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi372 – 372Interchain By similarityBy similarity
Glycosylationi398 – 3981N-linked (GlcNAc...) Reviewed prediction
Glycosylationi423 – 4231N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Gene expression databases

GenevestigatoriQ09131.

Interactioni

Subunit structurei

Homodimer; disulfide-linked By similarity.By similarity2 Publications

Structurei

3D structure databases

ProteinModelPortaliQ09131.
SMRiQ09131. Positions 37-459.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni350 – 3523Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view]
SUPFAMiSSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q09131-1 [UniParc]FASTAAdd to Basket

« Hide

MGVVEGLLAL ALVLSACVMC NGGSSSPFIR KVEKTVDMPL DSDVFAVPPG    50
YNAPQQVHIT QGDLVGKAVI VSWVTVDEPG SSEVHYWSEN SDKKKIAEGK 100
LVTYRFFNYS SGFIHHTTIR NLEYKTKYYY EVGLGNTTRQ FWFVTPPEIG 150
PDVPYTFGLI GDLGQSFDSN KTLSHYELNP RKGQTVLFVG DLSYADNYPN 200
HDNIRWDSWG RFTERSVAYQ PWIWTAGNHE NHFAPEIGET VPFKPYTHRY 250
HVPYKASQST SPFWYSIKRA SAHIIVLASY SAYGKYTPQY KWLEKELPKV 300
NRTETPWLIV LMHSPWYNSY NYHYMEGETM RVMYEPWFVQ YKVDVVFAGH 350
VHAYERSERV SNVAYNIVNG LCAPVNDKSA PVYITIGDGG TLEGLATNMT 400
EPQPKYSAFR EASFGHAIFD ITNRTHAHYS WHRNQDGVAV EADSLWSFNR 450
YWHPVDDSTA HVSH 464
Length:464
Mass (Da):53,027
Last modified:December 6, 2005 - v2
Checksum:i83BBAD593BEB01F5
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 351T → A AA sequence 1 Publication
Sequence conflicti47 – 471V → R AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF200824 mRNA. Translation: AAF19820.1.
PIRiB59200.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF200824 mRNA. Translation: AAF19820.1 .
PIRi B59200.

3D structure databases

ProteinModelPortali Q09131.
SMRi Q09131. Positions 37-459.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-15155.

Gene expression databases

Genevestigatori Q09131.

Family and domain databases

Gene3Di 2.60.40.380. 1 hit.
3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR008963. Purple_acid_Pase-like_N.
IPR015914. Purple_acid_Pase_N.
IPR025733. Purple_acid_PPase_C_dom.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
PF14008. Metallophos_C. 1 hit.
[Graphical view ]
SUPFAMi SSF49363. SSF49363. 1 hit.
SSF56300. SSF56300. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Binuclear metal centers in plant purple acid phosphatases: Fe-Mn in sweet potato and Fe-Zn in soybean."
    Schenk G., Ge Y., Carrington L.E., Wynne C.J., Searle I.R., Carroll B.J., Hamilton S., de Jersey J.
    Arch. Biochem. Biophys. 370:183-189(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: cv. Provar.
  2. "Purification and characterization of a secreted purple phosphatase from soybean suspension cultures."
    Lebansky B.R., McKnight T.D., Griffing L.R.
    Plant Physiol. 99:391-395(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 31-47, CATALYTIC ACTIVITY, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiPPAF_SOYBN
AccessioniPrimary (citable) accession number: Q09131
Secondary accession number(s): Q9SE01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: July 9, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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