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Protein

Eukaryotic translation initiation factor 2 subunit gamma

Gene

tif213

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi30 – 378GTPBy similarity
Nucleotide bindingi114 – 1185GTPBy similarity
Nucleotide bindingi170 – 1734GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: GO_Central
  • GTP binding Source: UniProtKB-KW
  • translation initiation factor activity Source: PomBase

GO - Biological processi

  • cytoplasmic translational initiation Source: PomBase
  • formation of translation preinitiation complex Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SPO-72649. Translation initiation complex formation.
R-SPO-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SPO-72702. Ribosomal scanning and start codon recognition.
R-SPO-72731. Recycling of eIF2:GDP.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2 subunit gamma
Short name:
eIF-2-gamma
Gene namesi
Name:tif213
ORF Names:SPBC17G9.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC17G9.09.
PomBaseiSPBC17G9.09. tif213.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • eukaryotic translation initiation factor 2 complex Source: PomBase
  • intracellular Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446Eukaryotic translation initiation factor 2 subunit gammaPRO_0000137447Add
BLAST

Proteomic databases

MaxQBiQ09130.
PRIDEiQ09130.

Interactioni

Subunit structurei

Heterotrimer composed of an alpha, a beta and a gamma chain.

Protein-protein interaction databases

BioGridi276290. 11 interactions.
MINTiMINT-4693606.

Structurei

3D structure databases

ProteinModelPortaliQ09130.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 227207tr-type GPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni30 – 378G1PROSITE-ProRule annotation
Regioni58 – 625G2PROSITE-ProRule annotation
Regioni114 – 1174G3PROSITE-ProRule annotation
Regioni170 – 1734G4PROSITE-ProRule annotation
Regioni205 – 2073G5PROSITE-ProRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi78 – 10023Cys-richAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EIF2G subfamily.PROSITE-ProRule annotation
Contains 1 tr-type G (guanine nucleotide-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000229292.
InParanoidiQ09130.
KOiK03242.
OMAiWTDRHSE.
OrthoDBiEOG092C1URX.
PhylomeDBiQ09130.

Family and domain databases

CDDicd15490. eIF2_gamma_III. 1 hit.
Gene3Di3.40.50.300. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR015256. TIF2_gsu_C.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PfamiPF09173. eIF2_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q09130-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAENLDISEL SPIHPAIISR QATINIGTIG HVAHGKSTVV KAISGVHTVR
60 70 80 90 100
FKNELERNIT IKLGYANAKI YKCSNEECPR PGCYRSYSSN KEDHPPCEIC
110 120 130 140 150
NSPMNLVRHV SFVDCPGHDI LMATMLNGAA VMDAALLLIA GNESCPQPQT
160 170 180 190 200
SEHLAAIEIM QLKHIIILQN KVDLIRESAA EEHYQSILKF IKGTVAENSP
210 220 230 240 250
IVPISAQLKY NIDAILEYIV KKIPIPVRDF TTAPRLIVIR SFDVNKPGAE
260 270 280 290 300
VDDLKGGVAG GSILTGVLRL NDEIEIRPGI VTKDDDGRIR CQPIFSRIIS
310 320 330 340 350
LFAEHNDLKI AVPGGLIGVG TTVDPTLCRA DRLVGQVLGS KGNLPEVYTE
360 370 380 390 400
LEINYFLLRR LLGVKSGDKN TTKVQKLAKN EVLMVNIGST STGGRVMMVK
410 420 430 440
ADMAKILLTA PACTEIGEKV ALSRRIEKHW RLIGWAKVVE GKTLKV
Length:446
Mass (Da):48,781
Last modified:November 1, 1997 - v1
Checksum:iF4B493F6EBF48139
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37354 mRNA. Translation: AAC49675.1.
CU329671 Genomic DNA. Translation: CAB52807.1.
PIRiT39732.
RefSeqiNP_595898.1. NM_001021805.2.

Genome annotation databases

EnsemblFungiiSPBC17G9.09.1; SPBC17G9.09.1:pep; SPBC17G9.09.
GeneIDi2539738.
KEGGispo:SPBC17G9.09.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37354 mRNA. Translation: AAC49675.1.
CU329671 Genomic DNA. Translation: CAB52807.1.
PIRiT39732.
RefSeqiNP_595898.1. NM_001021805.2.

3D structure databases

ProteinModelPortaliQ09130.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276290. 11 interactions.
MINTiMINT-4693606.

Proteomic databases

MaxQBiQ09130.
PRIDEiQ09130.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC17G9.09.1; SPBC17G9.09.1:pep; SPBC17G9.09.
GeneIDi2539738.
KEGGispo:SPBC17G9.09.

Organism-specific databases

EuPathDBiFungiDB:SPBC17G9.09.
PomBaseiSPBC17G9.09. tif213.

Phylogenomic databases

HOGENOMiHOG000229292.
InParanoidiQ09130.
KOiK03242.
OMAiWTDRHSE.
OrthoDBiEOG092C1URX.
PhylomeDBiQ09130.

Enzyme and pathway databases

ReactomeiR-SPO-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SPO-72649. Translation initiation complex formation.
R-SPO-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SPO-72702. Ribosomal scanning and start codon recognition.
R-SPO-72731. Recycling of eIF2:GDP.

Miscellaneous databases

PROiQ09130.

Family and domain databases

CDDicd15490. eIF2_gamma_III. 1 hit.
Gene3Di3.40.50.300. 1 hit.
InterProiIPR004161. EFTu-like_2.
IPR027417. P-loop_NTPase.
IPR000795. TF_GTP-bd_dom.
IPR015256. TIF2_gsu_C.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PfamiPF09173. eIF2_C. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF2G_SCHPO
AccessioniPrimary (citable) accession number: Q09130
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.