Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Eukaryotic translation initiation factor 2 subunit gamma

Gene

tif213

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 37GTPBy similarity8
Nucleotide bindingi114 – 118GTPBy similarity5
Nucleotide bindingi170 – 173GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • translation initiation factor activity Source: PomBase

GO - Biological processi

  • formation of translation preinitiation complex Source: PomBase

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-SPO-72649 Translation initiation complex formation
R-SPO-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-SPO-72702 Ribosomal scanning and start codon recognition
R-SPO-72731 Recycling of eIF2:GDP

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2 subunit gamma
Short name:
eIF-2-gamma
Gene namesi
Name:tif213
ORF Names:SPBC17G9.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC17G9.09
PomBaseiSPBC17G9.09 tif213

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001374471 – 446Eukaryotic translation initiation factor 2 subunit gammaAdd BLAST446

Proteomic databases

MaxQBiQ09130
PaxDbiQ09130
PRIDEiQ09130

Interactioni

Subunit structurei

Heterotrimer composed of an alpha, a beta and a gamma chain.

Protein-protein interaction databases

BioGridi276290, 12 interactors
STRINGi4896.SPBC17G9.09.1

Structurei

3D structure databases

ProteinModelPortaliQ09130
SMRiQ09130
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 227tr-type GPROSITE-ProRule annotationAdd BLAST207

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni30 – 37G1PROSITE-ProRule annotation8
Regioni58 – 62G2PROSITE-ProRule annotation5
Regioni114 – 117G3PROSITE-ProRule annotation4
Regioni170 – 173G4PROSITE-ProRule annotation4
Regioni205 – 207G5PROSITE-ProRule annotation3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi78 – 100Cys-richAdd BLAST23

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EIF2G subfamily.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000229292
InParanoidiQ09130
KOiK03242
OMAiCPQPQTK
OrthoDBiEOG092C1URX
PhylomeDBiQ09130

Family and domain databases

CDDicd15490 eIF2_gamma_III, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR027417 P-loop_NTPase
IPR000795 TF_GTP-bd_dom
IPR015256 TIF2_gsu_C
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
PfamiView protein in Pfam
PF09173 eIF2_C, 1 hit
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q09130-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAENLDISEL SPIHPAIISR QATINIGTIG HVAHGKSTVV KAISGVHTVR
60 70 80 90 100
FKNELERNIT IKLGYANAKI YKCSNEECPR PGCYRSYSSN KEDHPPCEIC
110 120 130 140 150
NSPMNLVRHV SFVDCPGHDI LMATMLNGAA VMDAALLLIA GNESCPQPQT
160 170 180 190 200
SEHLAAIEIM QLKHIIILQN KVDLIRESAA EEHYQSILKF IKGTVAENSP
210 220 230 240 250
IVPISAQLKY NIDAILEYIV KKIPIPVRDF TTAPRLIVIR SFDVNKPGAE
260 270 280 290 300
VDDLKGGVAG GSILTGVLRL NDEIEIRPGI VTKDDDGRIR CQPIFSRIIS
310 320 330 340 350
LFAEHNDLKI AVPGGLIGVG TTVDPTLCRA DRLVGQVLGS KGNLPEVYTE
360 370 380 390 400
LEINYFLLRR LLGVKSGDKN TTKVQKLAKN EVLMVNIGST STGGRVMMVK
410 420 430 440
ADMAKILLTA PACTEIGEKV ALSRRIEKHW RLIGWAKVVE GKTLKV
Length:446
Mass (Da):48,781
Last modified:November 1, 1997 - v1
Checksum:iF4B493F6EBF48139
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37354 mRNA Translation: AAC49675.1
CU329671 Genomic DNA Translation: CAB52807.1
PIRiT39732
RefSeqiNP_595898.1, NM_001021805.2

Genome annotation databases

EnsemblFungiiSPBC17G9.09.1; SPBC17G9.09.1:pep; SPBC17G9.09
GeneIDi2539738
KEGGispo:SPBC17G9.09

Similar proteinsi

Entry informationi

Entry nameiIF2G_SCHPO
AccessioniPrimary (citable) accession number: Q09130
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 132 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health