1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial precursor

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Reviewed, UniProtKB/Swiss-Prot Q09128 (CP24A_RAT)

Last modified June 16, 2009. Version 64. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
      Short name=Vitamin D(3) 24-hydroxylase
      Short name=24-OHase
    EC=1.14.13.n4
Alternative name(s):
    Cytochrome P450 24A1
    P450-CC24

Gene names

Name:	Cyp24a1
Synonyms:	Cyp24

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D₃) and calcitriol (1-alpha,25-dihydroxyvitamin D₃). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid.
Catalytic activity	Calcitriol + NADPH + O₂ = calcitetrol + NADP⁺ + H₂O. Ref.5 Calcidiol + NADPH + O₂ = secalciferol + NADP⁺ + H₂O. Ref.5
Cofactor	Heme group By similarity.
Subcellular location	Mitochondrion.
Sequence similarities	Belongs to the cytochrome P450 family.
biophysicochemical properties	Kinetic parameters: K_M=2.8 µM for calcidiol pH dependence: Optimum pH is 7.7.

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Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW vitamin D catabolic process Traceable author statement. Source: RGD
Cellular component	mitochondrion Traceable author statement. Source: RGD
Molecular function	1-alpha,25-dihydroxyvitamin D3 24-hydroxylase activity Traceable author statement. Source: UniProtKB 25-hydroxycholecalciferol-24-hydroxylase activity Ref.1 Inferred from direct assay. Source: RGD electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen Inferred from electronic annotation. Source: EC
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 35

Mitochondrion Ref.5 Ref.1

Chain

36 – 514

479

1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial

PRO_0000003617

Sites

Metal binding

462

Iron (heme axial ligand) Potential

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Sequences

Sequence

Length

Mass (Da)

Tools

Q09128-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 2E5CC1CCBA3C2B91
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FASTA

514

59,448

        10         20         30         40         50         60 
MSCPIDKRRT LIAFLRRLRD LGQPPRSVTS KASASRAPKE VPLCPLMTDG ETRNVTSLPG 

        70         80         90        100        110        120 
PTNWPLLGSL LEIFWKGGLK KQHDTLAEYH KKYGQIFRMK LGSFDSVHLG SPSLLEALYR 

       130        140        150        160        170        180 
TESAHPQRLE IKPWKAYRDH RNEAYGLMIL EGQEWQRVRS AFQKKLMKPV EIMKLDKKIN 

       190        200        210        220        230        240 
EVLADFLERM DELCDERGRI PDLYSELNKW SFESICLVLY EKRFGLLQKE TEEEALTFIT 

       250        260        270        280        290        300 
AIKTMMSTFG KMMVTPVELH KRLNTKVWQA HTLAWDTIFK SVKPCIDNRL QRYSQQPGAD 

       310        320        330        340        350        360 
FLCDIYQQDH LSKKELYAAV TELQLAAVET TANSLMWILY NLSRNPQAQR RLLQEVQSVL 

       370        380        390        400        410        420 
PDNQTPRAED LRNMPYLKAC LKESMRLTPS VPFTTRTLDK PTVLGEYALP KGTVLTLNTQ 

       430        440        450        460        470        480 
VLGSSEDNFE DSHKFRPERW LQKEKKINPF AHLPFGIGKR MCIGRRLAEL QLHLALCWII 

       490        500        510 
QKYDIVATDN EPVEMLHLGI LVPSRELPIA FRPR

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of cDNA encoding 25-hydroxyvitamin D3 24-hydroxylase." Ohyama Y., Noshiro M., Okuda K. FEBS Lett. 278:195-198(1991) [PubMed: 1991512] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 36-43. Tissue: Kidney.
[2]	"Structural characterization of the gene encoding rat 25-hydroxyvitamin D3 24-hydroxylase." Ohyama Y., Noshiro M., Eggertsen G., Gotoh O., Kato Y., Bjoerkhem I., Okuda K. Biochemistry 32:76-82(1993) [PubMed: 8418863] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[4]	"Identification of a vitamin D responsive element in the promoter of the rat cytochrome P450(24) gene." Hahn C.N., Kerry D.M., Omdahl J.L., May B.K. Nucleic Acids Res. 22:2410-2416(1994) [PubMed: 8036172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-86.
[5]	"Isolation and characterization of a cytochrome P-450 from rat kidney mitochondria that catalyzes the 24-hydroxylation of 25-hydroxyvitamin D3." Ohyama Y., Okuda K. J. Biol. Chem. 266:8690-8695(1991) [PubMed: 2026586] [Abstract] Cited for: PROTEIN SEQUENCE OF 36-43, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES.
[6]	"Metabolism of vitamin D3 by cytochromes P450." Sakaki T., Kagawa N., Yamamoto K., Inouye K. Front. Biosci. 10:119-134(2005) [PubMed: 15574355] [Abstract] Cited for: SUBSTRATE SPECIFICITY.

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Cross-references

Sequence databases
	X59506 mRNA. Translation: CAA42093.1. L04618 L04617 Genomic DNA. Translation: AAA42340.1. BC100059 mRNA. Translation: AAI00060.1. Z28351 Genomic DNA. Translation: CAA82206.1.
IPI	IPI00559048.
PIR	A45228.
RefSeq	NP_963966.1.
UniGene	Rn.100353
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000013062. Rattus norvegicus. [Contig view]
GeneID	25279.
KEGG	rno:25279.
Organism-specific databases
RGD	2462. Cyp24a1.
Phylogenomic databases
HOVERGEN	Q09128.
OMA	Q09128. RFGLLQK.
Gene expression databases
ArrayExpress	Q09128.
GermOnline	ENSRNOG00000013062. Rattus norvegicus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002949. Cyt_P450_E_CYP24A_mit. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR01238. MITP450CC24. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	605991.

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Entry information

Entry name

CP24A_RAT

Accession

Primary (citable) accession number: Q09128
Secondary accession number(s): Q498V4, Q63685

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents