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Q09128

- CP24A_RAT

UniProt

Q09128 - CP24A_RAT

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Protein

1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial

Gene

Cyp24a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D3) and calcitriol (1-alpha,25-dihydroxyvitamin D3). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid.

Catalytic activityi

Calcitriol + NADPH + O2 = calcitetrol + NADP+ + H2O.1 Publication
Calcidiol + NADPH + O2 = secalciferol + NADP+ + H2O.1 Publication

Cofactori

heme1 Publication

Kineticsi

  1. KM=2.8 µM for calcidiol1 Publication

pH dependencei

Optimum pH is 7.7.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi462 – 4621Iron (heme axial ligand)

GO - Molecular functioni

  1. 1-alpha,25-dihydroxyvitamin D3 24-hydroxylase activity Source: BHF-UCL
  2. 25-hydroxycholecalciferol-24-hydroxylase activity Source: RGD
  3. heme binding Source: InterPro
  4. iron ion binding Source: InterPro

GO - Biological processi

  1. osteoblast differentiation Source: Ensembl
  2. response to vitamin D Source: Ensembl
  3. vitamin D catabolic process Source: RGD
  4. vitamin D metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14357.
ReactomeiREACT_231451. Vitamin D (calciferol) metabolism.
REACT_258063. Vitamins.
SABIO-RKQ09128.

Names & Taxonomyi

Protein namesi
Recommended name:
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial (EC:1.14.13.126)
Short name:
24-OHase
Short name:
Vitamin D(3) 24-hydroxylase
Alternative name(s):
Cytochrome P450 24A1
Cytochrome P450-CC24
Gene namesi
Name:Cyp24a1
Synonyms:Cyp24
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 3

Organism-specific databases

RGDi2462. Cyp24a1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3535Mitochondrion2 PublicationsAdd
BLAST
Chaini36 – 5144791,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrialPRO_0000003617Add
BLAST

Proteomic databases

PRIDEiQ09128.

Expressioni

Gene expression databases

GenevestigatoriQ09128.

Interactioni

Structurei

Secondary structure

1
514
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi55 – 573Combined sources
Turni65 – 673Combined sources
Helixi70 – 756Combined sources
Helixi79 – 813Combined sources
Helixi82 – 9312Combined sources
Beta strandi95 – 1017Combined sources
Beta strandi104 – 1096Combined sources
Helixi112 – 1209Combined sources
Helixi132 – 14110Combined sources
Turni147 – 1493Combined sources
Helixi152 – 16615Combined sources
Helixi169 – 1724Combined sources
Helixi173 – 1753Combined sources
Helixi176 – 19318Combined sources
Beta strandi196 – 1983Combined sources
Helixi203 – 22018Combined sources
Beta strandi226 – 2283Combined sources
Helixi236 – 24611Combined sources
Helixi249 – 2524Combined sources
Beta strandi253 – 2553Combined sources
Helixi257 – 2637Combined sources
Helixi266 – 29227Combined sources
Turni293 – 2953Combined sources
Helixi301 – 3088Combined sources
Helixi313 – 34331Combined sources
Helixi346 – 35914Combined sources
Helixi368 – 3736Combined sources
Helixi375 – 38713Combined sources
Beta strandi393 – 3975Combined sources
Beta strandi402 – 4043Combined sources
Beta strandi407 – 4093Combined sources
Beta strandi414 – 4185Combined sources
Helixi421 – 4244Combined sources
Turni426 – 4283Combined sources
Beta strandi432 – 4343Combined sources
Helixi437 – 4404Combined sources
Helixi449 – 4513Combined sources
Helixi465 – 48218Combined sources
Beta strandi483 – 4886Combined sources
Beta strandi495 – 50612Combined sources
Beta strandi509 – 5135Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3K9VX-ray2.50A/B34-514[»]
3K9YX-ray2.80A/B34-514[»]
ProteinModelPortaliQ09128.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ09128.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000119243.
HOGENOMiHOG000276540.
HOVERGENiHBG099053.
InParanoidiQ09128.
KOiK07436.
OMAiQHDTLAE.
OrthoDBiEOG7ZGX4B.
PhylomeDBiQ09128.
TreeFamiTF105094.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002949. Cyt_P450_E_CYP24A_mit.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR01238. MITP450CC24.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q09128-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSCPIDKRRT LIAFLRRLRD LGQPPRSVTS KASASRAPKE VPLCPLMTDG
60 70 80 90 100
ETRNVTSLPG PTNWPLLGSL LEIFWKGGLK KQHDTLAEYH KKYGQIFRMK
110 120 130 140 150
LGSFDSVHLG SPSLLEALYR TESAHPQRLE IKPWKAYRDH RNEAYGLMIL
160 170 180 190 200
EGQEWQRVRS AFQKKLMKPV EIMKLDKKIN EVLADFLERM DELCDERGRI
210 220 230 240 250
PDLYSELNKW SFESICLVLY EKRFGLLQKE TEEEALTFIT AIKTMMSTFG
260 270 280 290 300
KMMVTPVELH KRLNTKVWQA HTLAWDTIFK SVKPCIDNRL QRYSQQPGAD
310 320 330 340 350
FLCDIYQQDH LSKKELYAAV TELQLAAVET TANSLMWILY NLSRNPQAQR
360 370 380 390 400
RLLQEVQSVL PDNQTPRAED LRNMPYLKAC LKESMRLTPS VPFTTRTLDK
410 420 430 440 450
PTVLGEYALP KGTVLTLNTQ VLGSSEDNFE DSHKFRPERW LQKEKKINPF
460 470 480 490 500
AHLPFGIGKR MCIGRRLAEL QLHLALCWII QKYDIVATDN EPVEMLHLGI
510
LVPSRELPIA FRPR
Length:514
Mass (Da):59,448
Last modified:October 1, 1996 - v1
Checksum:i2E5CC1CCBA3C2B91
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59506 mRNA. Translation: CAA42093.1.
L04618
, L04608, L04609, L04610, L04611, L04612, L04613, L04614, L04615, L04616, L04617 Genomic DNA. Translation: AAA42340.1.
BC100059 mRNA. Translation: AAI00060.1.
Z28351 Genomic DNA. Translation: CAA82206.1.
PIRiA45228.
RefSeqiNP_963966.1. NM_201635.2.
XP_006235734.1. XM_006235672.2.
UniGeneiRn.100353.

Genome annotation databases

EnsembliENSRNOT00000046011; ENSRNOP00000043298; ENSRNOG00000013062.
GeneIDi25279.
KEGGirno:25279.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59506 mRNA. Translation: CAA42093.1 .
L04618
, L04608 , L04609 , L04610 , L04611 , L04612 , L04613 , L04614 , L04615 , L04616 , L04617 Genomic DNA. Translation: AAA42340.1 .
BC100059 mRNA. Translation: AAI00060.1 .
Z28351 Genomic DNA. Translation: CAA82206.1 .
PIRi A45228.
RefSeqi NP_963966.1. NM_201635.2.
XP_006235734.1. XM_006235672.2.
UniGenei Rn.100353.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3K9V X-ray 2.50 A/B 34-514 [» ]
3K9Y X-ray 2.80 A/B 34-514 [» ]
ProteinModelPortali Q09128.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi Q09128.
ChEMBLi CHEMBL3748.

Proteomic databases

PRIDEi Q09128.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000046011 ; ENSRNOP00000043298 ; ENSRNOG00000013062 .
GeneIDi 25279.
KEGGi rno:25279.

Organism-specific databases

CTDi 1591.
RGDi 2462. Cyp24a1.

Phylogenomic databases

eggNOGi COG2124.
GeneTreei ENSGT00760000119243.
HOGENOMi HOG000276540.
HOVERGENi HBG099053.
InParanoidi Q09128.
KOi K07436.
OMAi QHDTLAE.
OrthoDBi EOG7ZGX4B.
PhylomeDBi Q09128.
TreeFami TF105094.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-14357.
Reactomei REACT_231451. Vitamin D (calciferol) metabolism.
REACT_258063. Vitamins.
SABIO-RK Q09128.

Miscellaneous databases

EvolutionaryTracei Q09128.
NextBioi 605991.
PROi Q09128.

Gene expression databases

Genevestigatori Q09128.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002949. Cyt_P450_E_CYP24A_mit.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR01238. MITP450CC24.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of cDNA encoding 25-hydroxyvitamin D3 24-hydroxylase."
    Ohyama Y., Noshiro M., Okuda K.
    FEBS Lett. 278:195-198(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 36-43.
    Tissue: Kidney.
  2. "Structural characterization of the gene encoding rat 25-hydroxyvitamin D3 24-hydroxylase."
    Ohyama Y., Noshiro M., Eggertsen G., Gotoh O., Kato Y., Bjoerkhem I., Okuda K.
    Biochemistry 32:76-82(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  4. "Identification of a vitamin D responsive element in the promoter of the rat cytochrome P450(24) gene."
    Hahn C.N., Kerry D.M., Omdahl J.L., May B.K.
    Nucleic Acids Res. 22:2410-2416(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-86.
  5. "Isolation and characterization of a cytochrome P-450 from rat kidney mitochondria that catalyzes the 24-hydroxylation of 25-hydroxyvitamin D3."
    Ohyama Y., Okuda K.
    J. Biol. Chem. 266:8690-8695(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 36-43, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES.
  6. "Metabolism of vitamin D3 by cytochromes P450."
    Sakaki T., Kagawa N., Yamamoto K., Inouye K.
    Front. Biosci. 10:119-134(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBSTRATE SPECIFICITY.
  7. "Crystal structure of CYP24A1, a mitochondrial cytochrome P450 involved in vitamin D metabolism."
    Annalora A.J., Goodin D.B., Hong W.X., Zhang Q., Johnson E.F., Stout C.D.
    J. Mol. Biol. 396:441-451(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 34-514 IN COMPLEX WITH HEME, COFACTOR.

Entry informationi

Entry nameiCP24A_RAT
AccessioniPrimary (citable) accession number: Q09128
Secondary accession number(s): Q498V4, Q63685
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3