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Q09128

- CP24A_RAT

UniProt

Q09128 - CP24A_RAT

Protein

1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial

Gene

Cyp24a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D3) and calcitriol (1-alpha,25-dihydroxyvitamin D3). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid.

    Catalytic activityi

    Calcitriol + NADPH + O2 = calcitetrol + NADP+ + H2O.1 Publication
    Calcidiol + NADPH + O2 = secalciferol + NADP+ + H2O.1 Publication

    Cofactori

    Heme group.1 Publication

    Kineticsi

    1. KM=2.8 µM for calcidiol1 Publication

    pH dependencei

    Optimum pH is 7.7.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi462 – 4621Iron (heme axial ligand)

    GO - Molecular functioni

    1. 1-alpha,25-dihydroxyvitamin D3 24-hydroxylase activity Source: BHF-UCL
    2. 25-hydroxycholecalciferol-24-hydroxylase activity Source: RGD
    3. heme binding Source: InterPro
    4. iron ion binding Source: InterPro

    GO - Biological processi

    1. osteoblast differentiation Source: Ensembl
    2. response to vitamin D Source: Ensembl
    3. vitamin D catabolic process Source: RGD
    4. vitamin D metabolic process Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14357.
    SABIO-RKQ09128.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial (EC:1.14.13.126)
    Short name:
    24-OHase
    Short name:
    Vitamin D(3) 24-hydroxylase
    Alternative name(s):
    Cytochrome P450 24A1
    Cytochrome P450-CC24
    Gene namesi
    Name:Cyp24a1
    Synonyms:Cyp24
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi2462. Cyp24a1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrion Source: RGD

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3535Mitochondrion2 PublicationsAdd
    BLAST
    Chaini36 – 5144791,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrialPRO_0000003617Add
    BLAST

    Proteomic databases

    PRIDEiQ09128.

    Expressioni

    Gene expression databases

    GenevestigatoriQ09128.

    Interactioni

    Structurei

    Secondary structure

    1
    514
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi55 – 573
    Turni65 – 673
    Helixi70 – 756
    Helixi79 – 813
    Helixi82 – 9312
    Beta strandi95 – 1017
    Beta strandi104 – 1096
    Helixi112 – 1209
    Helixi132 – 14110
    Turni147 – 1493
    Helixi152 – 16615
    Helixi169 – 1724
    Helixi173 – 1753
    Helixi176 – 19318
    Beta strandi196 – 1983
    Helixi203 – 22018
    Beta strandi226 – 2283
    Helixi236 – 24611
    Helixi249 – 2524
    Beta strandi253 – 2553
    Helixi257 – 2637
    Helixi266 – 29227
    Turni293 – 2953
    Helixi301 – 3088
    Helixi313 – 34331
    Helixi346 – 35914
    Helixi368 – 3736
    Helixi375 – 38713
    Beta strandi393 – 3975
    Beta strandi402 – 4043
    Beta strandi407 – 4093
    Beta strandi414 – 4185
    Helixi421 – 4244
    Turni426 – 4283
    Beta strandi432 – 4343
    Helixi437 – 4404
    Helixi449 – 4513
    Helixi465 – 48218
    Beta strandi483 – 4886
    Beta strandi495 – 50612
    Beta strandi509 – 5135

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3K9VX-ray2.50A/B34-514[»]
    3K9YX-ray2.80A/B34-514[»]
    ProteinModelPortaliQ09128.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ09128.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG2124.
    GeneTreeiENSGT00750000117435.
    HOGENOMiHOG000276540.
    HOVERGENiHBG099053.
    InParanoidiQ09128.
    KOiK07436.
    OMAiQHDTLAE.
    OrthoDBiEOG7ZGX4B.
    PhylomeDBiQ09128.
    TreeFamiTF105094.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002949. Cyt_P450_E_CYP24A_mit.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR01238. MITP450CC24.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q09128-1 [UniParc]FASTAAdd to Basket

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    MSCPIDKRRT LIAFLRRLRD LGQPPRSVTS KASASRAPKE VPLCPLMTDG    50
    ETRNVTSLPG PTNWPLLGSL LEIFWKGGLK KQHDTLAEYH KKYGQIFRMK 100
    LGSFDSVHLG SPSLLEALYR TESAHPQRLE IKPWKAYRDH RNEAYGLMIL 150
    EGQEWQRVRS AFQKKLMKPV EIMKLDKKIN EVLADFLERM DELCDERGRI 200
    PDLYSELNKW SFESICLVLY EKRFGLLQKE TEEEALTFIT AIKTMMSTFG 250
    KMMVTPVELH KRLNTKVWQA HTLAWDTIFK SVKPCIDNRL QRYSQQPGAD 300
    FLCDIYQQDH LSKKELYAAV TELQLAAVET TANSLMWILY NLSRNPQAQR 350
    RLLQEVQSVL PDNQTPRAED LRNMPYLKAC LKESMRLTPS VPFTTRTLDK 400
    PTVLGEYALP KGTVLTLNTQ VLGSSEDNFE DSHKFRPERW LQKEKKINPF 450
    AHLPFGIGKR MCIGRRLAEL QLHLALCWII QKYDIVATDN EPVEMLHLGI 500
    LVPSRELPIA FRPR 514
    Length:514
    Mass (Da):59,448
    Last modified:October 1, 1996 - v1
    Checksum:i2E5CC1CCBA3C2B91
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59506 mRNA. Translation: CAA42093.1.
    L04618
    , L04608, L04609, L04610, L04611, L04612, L04613, L04614, L04615, L04616, L04617 Genomic DNA. Translation: AAA42340.1.
    BC100059 mRNA. Translation: AAI00060.1.
    Z28351 Genomic DNA. Translation: CAA82206.1.
    PIRiA45228.
    RefSeqiNP_963966.1. NM_201635.2.
    XP_006235734.1. XM_006235672.1.
    UniGeneiRn.100353.

    Genome annotation databases

    EnsembliENSRNOT00000046011; ENSRNOP00000043298; ENSRNOG00000013062.
    GeneIDi25279.
    KEGGirno:25279.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59506 mRNA. Translation: CAA42093.1 .
    L04618
    , L04608 , L04609 , L04610 , L04611 , L04612 , L04613 , L04614 , L04615 , L04616 , L04617 Genomic DNA. Translation: AAA42340.1 .
    BC100059 mRNA. Translation: AAI00060.1 .
    Z28351 Genomic DNA. Translation: CAA82206.1 .
    PIRi A45228.
    RefSeqi NP_963966.1. NM_201635.2.
    XP_006235734.1. XM_006235672.1.
    UniGenei Rn.100353.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3K9V X-ray 2.50 A/B 34-514 [» ]
    3K9Y X-ray 2.80 A/B 34-514 [» ]
    ProteinModelPortali Q09128.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q09128.
    ChEMBLi CHEMBL3748.

    Proteomic databases

    PRIDEi Q09128.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000046011 ; ENSRNOP00000043298 ; ENSRNOG00000013062 .
    GeneIDi 25279.
    KEGGi rno:25279.

    Organism-specific databases

    CTDi 1591.
    RGDi 2462. Cyp24a1.

    Phylogenomic databases

    eggNOGi COG2124.
    GeneTreei ENSGT00750000117435.
    HOGENOMi HOG000276540.
    HOVERGENi HBG099053.
    InParanoidi Q09128.
    KOi K07436.
    OMAi QHDTLAE.
    OrthoDBi EOG7ZGX4B.
    PhylomeDBi Q09128.
    TreeFami TF105094.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-14357.
    SABIO-RK Q09128.

    Miscellaneous databases

    EvolutionaryTracei Q09128.
    NextBioi 605991.
    PROi Q09128.

    Gene expression databases

    Genevestigatori Q09128.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002949. Cyt_P450_E_CYP24A_mit.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR01238. MITP450CC24.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of cDNA encoding 25-hydroxyvitamin D3 24-hydroxylase."
      Ohyama Y., Noshiro M., Okuda K.
      FEBS Lett. 278:195-198(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 36-43.
      Tissue: Kidney.
    2. "Structural characterization of the gene encoding rat 25-hydroxyvitamin D3 24-hydroxylase."
      Ohyama Y., Noshiro M., Eggertsen G., Gotoh O., Kato Y., Bjoerkhem I., Okuda K.
      Biochemistry 32:76-82(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    4. "Identification of a vitamin D responsive element in the promoter of the rat cytochrome P450(24) gene."
      Hahn C.N., Kerry D.M., Omdahl J.L., May B.K.
      Nucleic Acids Res. 22:2410-2416(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-86.
    5. "Isolation and characterization of a cytochrome P-450 from rat kidney mitochondria that catalyzes the 24-hydroxylation of 25-hydroxyvitamin D3."
      Ohyama Y., Okuda K.
      J. Biol. Chem. 266:8690-8695(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 36-43, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES.
    6. "Metabolism of vitamin D3 by cytochromes P450."
      Sakaki T., Kagawa N., Yamamoto K., Inouye K.
      Front. Biosci. 10:119-134(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBSTRATE SPECIFICITY.
    7. "Crystal structure of CYP24A1, a mitochondrial cytochrome P450 involved in vitamin D metabolism."
      Annalora A.J., Goodin D.B., Hong W.X., Zhang Q., Johnson E.F., Stout C.D.
      J. Mol. Biol. 396:441-451(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 34-514 IN COMPLEX WITH HEME, COFACTOR.

    Entry informationi

    Entry nameiCP24A_RAT
    AccessioniPrimary (citable) accession number: Q09128
    Secondary accession number(s): Q498V4, Q63685
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3