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Protein

1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial

Gene

Cyp24a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D3) and calcitriol (1-alpha,25-dihydroxyvitamin D3). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid.1 Publication

Catalytic activityi

Calcitriol + 2 reduced adrenodoxin + 2 H+ + O2 = calcitetrol + 2 oxidized adrenodoxin + H2O.1 Publication
Calcidiol + 2 reduced adrenodoxin + 2 H+ + O2 = secalciferol + 2 oxidized adrenodoxin + H2O.1 Publication

Cofactori

heme1 Publication

Kineticsi

  1. KM=2.8 µM for calcidiol1 Publication

    pH dependencei

    Optimum pH is 7.7.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi462Iron (heme axial ligand)1

    GO - Molecular functioni

    • 1-alpha,25-dihydroxyvitamin D3 24-hydroxylase activity Source: BHF-UCL
    • 25-hydroxycholecalciferol-24-hydroxylase activity Source: RGD
    • heme binding Source: InterPro
    • iron ion binding Source: InterPro

    GO - Biological processi

    • osteoblast differentiation Source: Ensembl
    • response to vitamin D Source: Ensembl
    • vitamin D catabolic process Source: RGD
    • vitamin D metabolic process Source: RGD
    Complete GO annotation...

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14357.
    ReactomeiR-RNO-196791. Vitamin D (calciferol) metabolism.
    R-RNO-211916. Vitamins.
    SABIO-RKQ09128.

    Chemistry databases

    SwissLipidsiSLP:000001482.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial (EC:1.14.15.161 Publication)
    Short name:
    24-OHase
    Short name:
    Vitamin D(3) 24-hydroxylase
    Alternative name(s):
    Cytochrome P450 24A1
    Cytochrome P450-CC24
    Gene namesi
    Name:Cyp24a1
    Synonyms:Cyp24
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Chromosome 3

    Organism-specific databases

    RGDi2462. Cyp24a1.

    Subcellular locationi

    GO - Cellular componenti

    • mitochondrion Source: RGD
    • nucleoplasm Source: Ensembl
    • plasma membrane Source: Ensembl
    Complete GO annotation...

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Chemistry databases

    ChEMBLiCHEMBL3748.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transit peptidei1 – 35Mitochondrion2 PublicationsAdd BLAST35
    ChainiPRO_000000361736 – 5141,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrialAdd BLAST479

    Proteomic databases

    PaxDbiQ09128.
    PRIDEiQ09128.

    Expressioni

    Gene expression databases

    BgeeiENSRNOG00000013062.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000043298.

    Chemistry databases

    BindingDBiQ09128.

    Structurei

    Secondary structure

    1514
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi55 – 57Combined sources3
    Turni65 – 67Combined sources3
    Helixi70 – 75Combined sources6
    Helixi79 – 81Combined sources3
    Helixi82 – 93Combined sources12
    Beta strandi95 – 101Combined sources7
    Beta strandi104 – 109Combined sources6
    Helixi112 – 120Combined sources9
    Helixi132 – 141Combined sources10
    Turni147 – 149Combined sources3
    Helixi152 – 166Combined sources15
    Helixi169 – 172Combined sources4
    Helixi173 – 175Combined sources3
    Helixi176 – 193Combined sources18
    Beta strandi196 – 198Combined sources3
    Helixi203 – 220Combined sources18
    Beta strandi226 – 228Combined sources3
    Helixi236 – 246Combined sources11
    Helixi249 – 252Combined sources4
    Beta strandi253 – 255Combined sources3
    Helixi257 – 263Combined sources7
    Helixi266 – 292Combined sources27
    Turni293 – 295Combined sources3
    Helixi301 – 308Combined sources8
    Helixi313 – 343Combined sources31
    Helixi346 – 359Combined sources14
    Helixi368 – 373Combined sources6
    Helixi375 – 387Combined sources13
    Beta strandi393 – 397Combined sources5
    Beta strandi402 – 404Combined sources3
    Beta strandi407 – 409Combined sources3
    Beta strandi414 – 418Combined sources5
    Helixi421 – 424Combined sources4
    Turni426 – 428Combined sources3
    Beta strandi432 – 434Combined sources3
    Helixi437 – 440Combined sources4
    Helixi449 – 451Combined sources3
    Helixi465 – 482Combined sources18
    Beta strandi483 – 488Combined sources6
    Beta strandi495 – 506Combined sources12
    Beta strandi509 – 513Combined sources5

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3K9VX-ray2.50A/B34-514[»]
    3K9YX-ray2.80A/B34-514[»]
    ProteinModelPortaliQ09128.
    SMRiQ09128.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ09128.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiKOG0159. Eukaryota.
    COG2124. LUCA.
    GeneTreeiENSGT00760000119243.
    HOGENOMiHOG000276540.
    HOVERGENiHBG099053.
    InParanoidiQ09128.
    KOiK07436.
    OMAiDYREEGY.
    OrthoDBiEOG091G0MI3.
    PhylomeDBiQ09128.
    TreeFamiTF105094.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002949. Cyt_P450_E_CYP24A_mit.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR01238. MITP450CC24.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q09128-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSCPIDKRRT LIAFLRRLRD LGQPPRSVTS KASASRAPKE VPLCPLMTDG
    60 70 80 90 100
    ETRNVTSLPG PTNWPLLGSL LEIFWKGGLK KQHDTLAEYH KKYGQIFRMK
    110 120 130 140 150
    LGSFDSVHLG SPSLLEALYR TESAHPQRLE IKPWKAYRDH RNEAYGLMIL
    160 170 180 190 200
    EGQEWQRVRS AFQKKLMKPV EIMKLDKKIN EVLADFLERM DELCDERGRI
    210 220 230 240 250
    PDLYSELNKW SFESICLVLY EKRFGLLQKE TEEEALTFIT AIKTMMSTFG
    260 270 280 290 300
    KMMVTPVELH KRLNTKVWQA HTLAWDTIFK SVKPCIDNRL QRYSQQPGAD
    310 320 330 340 350
    FLCDIYQQDH LSKKELYAAV TELQLAAVET TANSLMWILY NLSRNPQAQR
    360 370 380 390 400
    RLLQEVQSVL PDNQTPRAED LRNMPYLKAC LKESMRLTPS VPFTTRTLDK
    410 420 430 440 450
    PTVLGEYALP KGTVLTLNTQ VLGSSEDNFE DSHKFRPERW LQKEKKINPF
    460 470 480 490 500
    AHLPFGIGKR MCIGRRLAEL QLHLALCWII QKYDIVATDN EPVEMLHLGI
    510
    LVPSRELPIA FRPR
    Length:514
    Mass (Da):59,448
    Last modified:October 1, 1996 - v1
    Checksum:i2E5CC1CCBA3C2B91
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X59506 mRNA. Translation: CAA42093.1.
    L04618
    , L04608, L04609, L04610, L04611, L04612, L04613, L04614, L04615, L04616, L04617 Genomic DNA. Translation: AAA42340.1.
    BC100059 mRNA. Translation: AAI00060.1.
    Z28351 Genomic DNA. Translation: CAA82206.1.
    PIRiA45228.
    RefSeqiNP_963966.1. NM_201635.3.
    UniGeneiRn.100353.

    Genome annotation databases

    EnsembliENSRNOT00000046011; ENSRNOP00000043298; ENSRNOG00000013062.
    GeneIDi25279.
    KEGGirno:25279.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X59506 mRNA. Translation: CAA42093.1.
    L04618
    , L04608, L04609, L04610, L04611, L04612, L04613, L04614, L04615, L04616, L04617 Genomic DNA. Translation: AAA42340.1.
    BC100059 mRNA. Translation: AAI00060.1.
    Z28351 Genomic DNA. Translation: CAA82206.1.
    PIRiA45228.
    RefSeqiNP_963966.1. NM_201635.3.
    UniGeneiRn.100353.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3K9VX-ray2.50A/B34-514[»]
    3K9YX-ray2.80A/B34-514[»]
    ProteinModelPortaliQ09128.
    SMRiQ09128.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000043298.

    Chemistry databases

    BindingDBiQ09128.
    ChEMBLiCHEMBL3748.
    SwissLipidsiSLP:000001482.

    Proteomic databases

    PaxDbiQ09128.
    PRIDEiQ09128.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSRNOT00000046011; ENSRNOP00000043298; ENSRNOG00000013062.
    GeneIDi25279.
    KEGGirno:25279.

    Organism-specific databases

    CTDi1591.
    RGDi2462. Cyp24a1.

    Phylogenomic databases

    eggNOGiKOG0159. Eukaryota.
    COG2124. LUCA.
    GeneTreeiENSGT00760000119243.
    HOGENOMiHOG000276540.
    HOVERGENiHBG099053.
    InParanoidiQ09128.
    KOiK07436.
    OMAiDYREEGY.
    OrthoDBiEOG091G0MI3.
    PhylomeDBiQ09128.
    TreeFamiTF105094.

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14357.
    ReactomeiR-RNO-196791. Vitamin D (calciferol) metabolism.
    R-RNO-211916. Vitamins.
    SABIO-RKQ09128.

    Miscellaneous databases

    EvolutionaryTraceiQ09128.
    PROiQ09128.

    Gene expression databases

    BgeeiENSRNOG00000013062.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002949. Cyt_P450_E_CYP24A_mit.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR01238. MITP450CC24.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCP24A_RAT
    AccessioniPrimary (citable) accession number: Q09128
    Secondary accession number(s): Q498V4, Q63685
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: November 2, 2016
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.