Reviewed, UniProtKB/Swiss-Prot Q09124 (SCRK_LACLA)

Names and origin

Protein names	Recommended name:     Fructokinase-1     EC=2.7.1.4 Alternative name(s):     Fructokinase I
Organism	Lactococcus lactis subsp. lactis (Streptococcus lactis)
Taxonomic identifier	1360 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	25 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + D-fructose = ADP + D-fructose 6-phosphate.
Cofactor	Magnesium.
Enzyme regulation	Inhibition by zinc ions Potential. Inactivated by EDTA.
Subunit structure	Homodimer.
Miscellaneous	Two isoforms, fructokinase I and II, have been found in this organism.
Sequence similarities	Belongs to the ROK (nagC/xylR) family.
biophysicochemical properties	pH dependence: Optimum pH is 7.0-8.0.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructokinase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›25	›25	Fructokinase-1		PRO_0000095684
Experimental info
	Non-terminal residue	25	1

Sequences

	Sequence		Length	Mass (Da)
	Q09124-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 9C2B771CE8D5A1E8	FASTA	25	2,702
	10 20 SVYYGSIEAG GTKFVLAIAD EHFNI

References

[1]

"Purification and properties of fructokinase I from Lactococcus lactis. Localization of scrK on the sucrose-nisin transposon Tn5306." Thompson J., Sackett D.L., Donkersloot J.A. J. Biol. Chem. 266:22626-22633(1991) [PubMed: 1658003] [Abstract] Cited for: PROTEIN SEQUENCE, CHARACTERIZATION. Strain: K1.

Cross-references

Sequence databases
PIR	A39319.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-13045.
BRENDA	2.7.1.4. 278870.
Family and domain databases
InterPro	IPR000600. ROK. [Graphical view]
PROSITE	PS01125. ROK. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCRK_LACLA

Accession

Primary (citable) accession number: Q09124

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 1, 1997
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)