Q09123 (SCRK_FUSMR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructokinase
EC=2.7.1.4

Gene names

Name:

scrK

Organism

Fusobacterium mortiferum

Taxonomic identifier

850 [NCBI]

Taxonomic lineage

Bacteria › Fusobacteria › Fusobacterales › Fusobacteriaceae › Fusobacterium

Protein attributes

Sequence length	27 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + D-fructose = ADP + D-fructose 6-phosphate. Ref.1
Cofactor	Divalent metal cations. Maximum activity was observed with magnesium. Ref.1
Enzyme regulation	Inhibition by zinc ions Potential. UniProtKB O05510
Subunit structure	Homodimer. Ref.1
Induction	Induced by sucrose. Ref.1
Sequence similarities	Belongs to the ROK (nagC/xylR) family.
Biophysicochemical properties	Kinetic parameters: KM=0.1 mM for ATP Ref.1 KM=1.8 mM for magnesium Ref.1 KM=0.4 mM for fructose Ref.1 Vmax=74 µmol/min/mg enzyme for fructose 6-phosphate Ref.1 pH dependence: Optimum pH is 6.5-7.5. Ref.1

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cellular carbohydrate catabolic process Inferred from direct assay Ref.1. Source: UniProtKB
Cellular component	cell Inferred from direct assay Ref.1. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructokinase activity Inferred from direct assay Ref.1. Source: UniProtKB metal ion binding Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›27	›27	Fructokinase		PRO_0000095682
Experimental info
	Non-terminal residue	27	1

Sequences

	Sequence		Length	Mass (Da)
	Q09123 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 4721ABEF2E7BDCE9	FASTA	27	2,824
	10 20 MIIGAVEAGG TKFVDGVGNE KGEIFER

References

[1]

"Sucrose fermentation by Fusobacterium mortiferum ATCC 25557: transport, catabolism, and products." Thompson J., Nguyen N.Y., Robrish S.A. J. Bacteriol. 174:3227-3235(1992) [PubMed: 1533618] [Abstract] Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, INDUCTION. Strain: ATCC 25557 / CCUG 14475.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
PROSITE	PS01125. ROK. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCRK_FUSMR

Accession

Primary (citable) accession number: Q09123

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	February 1, 1997
Last modified:	May 31, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program