Reviewed, UniProtKB/Swiss-Prot Q09122 (SCRB_FUSMR)

Names and origin

Protein names	Recommended name:     Sucrose-6-phosphate hydrolase       Short name=Sucrase     EC=3.2.1.26 Alternative name(s):     Invertase
Organism	Fusobacterium mortiferum
Taxonomic identifier	850 [NCBI]
Taxonomic lineage	Bacteria › Fusobacteria › Fusobacterales › Fusobacteriaceae › Fusobacterium

Protein attributes

Sequence length	33 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides. Ref.1
Pathway	Glycan biosynthesis; sucrose metabolism. Ref.1
Induction	Induced by sucrose. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 32 family. Ref.1
Biophysicochemical properties	Kinetic parameters: KM=40 mM for sucrose Ref.1 KM=0.28 mM for sucrose-6-phosphate Ref.1 Vmax=43 µmol/min/mg enzyme for sucrose Ref.1 Vmax=270 µmol/min/mg enzyme for sucrose 6-phosphate Ref.1 pH dependence: Optimum pH is 7.0. Ref.1

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cellular carbohydrate catabolic process Ref.1 Inferred from direct assay. Source: UniProtKB
Cellular component	cell Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	beta-fructofuranosidase activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›33	›33	Sucrose-6-phosphate hydrolase		PRO_0000169871
Regions
	Region	15 – 18	4	Substrate binding By similarity
Sites
	Active site	18	1	By similarity
Experimental info
	Non-terminal residue	33	1

Sequences

	Sequence		Length	Mass (Da)
	Q09122-1 [UniParc]. Last modified February 1, 1997. Version 1. Checksum: 54C9FAF53856B597	FASTA	33	4,143
	10 20 30 MDKKEYINRL NEQKPLQEMX SRDRYLXNFH LIP

References

[1]

"Sucrose fermentation by Fusobacterium mortiferum ATCC 25557: transport, catabolism, and products." Thompson J., Nguyen N.Y., Robrish S.A. J. Bacteriol. 174:3227-3235(1992) [PubMed: 1533618] [Abstract] Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, PATHWAY, INDUCTION. Strain: ATCC 25557 / CCUG 14475.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.1.26. 97866.
Family and domain databases
InterPro	IPR018053. Glyco_hydro_32_AS. [Graphical view]
PROSITE	PS00609. GLYCOSYL_HYDROL_F32. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SCRB_FUSMR

Accession

Primary (citable) accession number: Q09122

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	February 1, 1997
Last modified:	March 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)