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Q09103

- DGK2_DROME

UniProt

Q09103 - DGK2_DROME

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Protein
Eye-specific diacylglycerol kinase
Gene
rdgA, DGK2, CG34344
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Required for the maintenance of phospholipid turnover within the photoreceptor.1 Publication

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri591 – 64151Phorbol-ester/DAG-type 1
Add
BLAST
Zinc fingeri661 – 72464Phorbol-ester/DAG-type 2
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. NAD+ kinase activity Source: InterPro
  3. diacylglycerol kinase activity Source: FlyBase
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. actin filament organization Source: FlyBase
  2. deactivation of rhodopsin mediated signaling Source: FlyBase
  3. intracellular signal transduction Source: InterPro
  4. lipid phosphorylation Source: FlyBase
  5. phosphatidic acid biosynthetic process Source: FlyBase
  6. phosphatidylinositol biosynthetic process Source: FlyBase
  7. phosphorylation Source: FlyBase
  8. photoreceptor cell maintenance Source: FlyBase
  9. phototransduction Source: FlyBase
  10. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
  11. rhodopsin mediated signaling pathway Source: FlyBase
  12. sensory perception of smell Source: FlyBase
  13. sensory perception of sound Source: FlyBase
  14. thermotaxis Source: FlyBase
  15. visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

SABIO-RKQ09103.
SignaLinkiQ09103.

Names & Taxonomyi

Protein namesi
Recommended name:
Eye-specific diacylglycerol kinase (EC:2.7.1.107)
Short name:
DAG kinase 2
Short name:
DGK 2
Short name:
Diglyceride kinase 2
Alternative name(s):
Retinal degeneration A protein
Gene namesi
Name:rdgA
Synonyms:DGK2
ORF Names:CG34344
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome X

Organism-specific databases

FlyBaseiFBgn0261549. rdgA.

Subcellular locationi

GO - Cellular componenti

  1. membrane Source: UniProtKB-SubCell
  2. microtubule associated complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Flies exhibit photoreceptor cells that degenerate within a week after eclosion.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14571457Eye-specific diacylglycerol kinase
PRO_0000218474Add
BLAST

Proteomic databases

PaxDbiQ09103.

Expressioni

Tissue specificityi

Expressed specifically in adult eye.1 Publication

Gene expression databases

BgeeiQ09103.

Interactioni

Protein-protein interaction databases

BioGridi58282. 12 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ09103.
SMRiQ09103. Positions 1275-1443.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini808 – 944137DAGKc
Add
BLAST
Repeati1320 – 134930ANK 1
Add
BLAST
Repeati1353 – 138230ANK 2
Add
BLAST
Repeati1389 – 141830ANK 3
Add
BLAST
Repeati1422 – 145130ANK 4
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 65Poly-Gln
Compositional biasi24 – 3916Thr-rich
Add
BLAST
Compositional biasi110 – 1156Poly-Ser
Compositional biasi227 – 2315Poly-Glu
Compositional biasi763 – 77816Gly-rich
Add
BLAST

Sequence similaritiesi

Contains 4 ANK repeats.
Contains 1 DAGKc domain.

Keywords - Domaini

ANK repeat, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0666.
GeneTreeiENSGT00740000115137.
InParanoidiQ09103.
KOiK00901.
OMAiRRTICCM.
OrthoDBiEOG76HQ0Q.
PhylomeDBiQ09103.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
[Graphical view]
PfamiPF00023. Ank. 2 hits.
PF00130. C1_1. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 4 hits.
SM00109. C1. 1 hit.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50146. DAGK. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform A (identifier: Q09103-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MQQQQQPSID QLPEPTASTS NSATTKPTIA TATTSTTTTS GNNFHQQLQA     50
TTAATMQRLR TTFTRSRTPT GAEMKMQNSL EVPKQVRSAS FDEMQLESQR 100
ASSSLLKQQS SSSASADERS SEAGFLQVPL AAHQQRSHSF DSATASAGSD 150
DSGTFLEVPR RLKARRSSST KTPPPCIHCH YLEEYERRMT AEQRYFIDHR 200
ELTALSYSNT SSEASEDEDE VEGHNAEEEE EGSAAIEDAE EETTEAATEE 250
ADEDPRTEVE SEHDHDPDDD AALEMDIRIG NMSQGSSIEE SRARLPRQMR 300
RHTIGSSSVT SASEDEGLEG SDNGSPHFGN TLLPPQPTTP CGITFTLSPT 350
NGDYPSPPHL PLDPGSPPIS PCSSNSGRLP ALAPIISTPC SSADADDAGA 400
AMGLPVRARR RSISRQEAIF VEPTGNSLEN VSHEEVDNSN TKSSVDTADS 450
LDEASTMATC GSPGAAGGSG ASSSHHNAFV VRDIYLMVPD LKRDRAASVD 500
SCFSKLSSNA KTEELQPSAD GCFLTVPNIN ATRSRSVDIV LPTDEQARYK 550
ALSMTGSTVT YADGRTASAS NSRRPIRIVP DWTENAVQGE HYWKPTSASG 600
DLCCLNEECI KSGQRMKCSA CQLVAHHNCI PFVNEKSTLA CKPTYRDVGI 650
RQYREQTTTH HHWVHRKLEK GKCKQCGKFF PMKQAVQSKL FGSKEIVALA 700
CAWCHEIYHN KEACFNQAKI GEECRLGNYA PIIVPPSWIV KLPTKGNFKS 750
SIRVSNKNNA ASGSGGGGAG GGAGGGGGKS KKQTQRRQKG KEEKKEPRAF 800
IVKPIPSPEV IPVIVFINPK SGGNQGHKLL GKFQHLLNPR QVFDLTQGGP 850
KMGLDMFRKA PNLRVLACGG DGTVGWVLSV LDQIQPPLQP APAVGVLPLG 900
TGNDLARALG WGGGYTDEPI GKILREIGMS QCVLMDRWRV KVTPNDDVTD 950
DHVDRSKPNV PLNVINNYFS FGVDAHIALE FHEAREAHPE RFNSRLRNKM 1000
YYGQMGGKDL ILRQYRNLSQ WVTLECDGQD FTGKLRDAGC HAVLFLNIPS 1050
YGGGTHPWND SFGASKPSID DGLMEVVGLT TYQLPMLQAG MHGTCICQCR 1100
KARIITKRTI PMQVDGEACR VKPSVIEIEL LNKALMLSKR KHGRGDVQVN 1150
PLEKMQLHIL RVTMQQYEQY HYDKEMLRKL ANKLGQIEIE SQCDLEHVRN 1200
MLNTKFEESI SYPKVSQDWC FIDSCTAEHY FRIDRAQEHL HYICDIAIDE 1250
LYILDHEAAT MPQTPDQERS FAAFSQRQAQ NERRQMDQAQ GRGPGSTDED 1300
LQIGSKPIKV MKWKSPILEQ TSDAILLAAQ SGDLNMLRAL HEQGYSLQSV 1350
NKNGQTALHF ACKYNHRDIV KYIIASATRR LINMADKELG QTALHIAAEQ 1400
NRRDICVMLV AAGAHLDTLD SGGNTPMMVA FNKNANEIAT YLESKQGTQP 1450
VDGWLDD 1457
Length:1,457
Mass (Da):160,142
Last modified:June 21, 2005 - v2
Checksum:i8F4E33E9C1B665D1
GO
Isoform B (identifier: Q09103-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-116: MQQQQQPSID...KQQSSSSASA → MPERSISQRD...EDIEMLDYDT
     117-564: Missing.
     1315-1315: S → SNKDRLFSFNEDVFGCGFS
     1445-1457: KQGTQPVDGWLDD → QERFMHLEKQTRI

Note: No experimental confirmation available.

Show »
Length:1,027
Mass (Da):115,579
Checksum:i70B26B9A087A84F9
GO
Isoform C (identifier: Q09103-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-132: MQQQQQPSID...AGFLQVPLAA → MERLLHAVRE...SLGAWRRKRR
     133-565: Missing.

Note: No experimental confirmation available.

Show »
Length:1,024
Mass (Da):113,748
Checksum:iD8119DAF5644BDC4
GO

Sequence cautioni

The sequence AAQ22428.1 differs from that shown. Reason: Intron retention.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 132132MQQQQ…VPLAA → MERLLHAVREEFQTEDEYET EVDDEGNVLHRSSISSCSSS SSSSNTSSSSDGSNSTASQP LSPSLPQPRRRLQRSDSFGS VGGGVAGGVAGSGATGAGGV RRFRRSSIGMQRKSAFRQRK LDSLGAWRRKRR in isoform C.
VSP_030265Add
BLAST
Alternative sequencei1 – 116116MQQQQ…SSASA → MPERSISQRDLDEIEIESDE EEEELEQGVGLSTRSRRNRR GASDSPAASRARNATNGIQN RGRERERERERERSRERFGG TNAADEARFYDDEEQRMEDD GEEDSDEDIEMLDYDT in isoform B.
VSP_030264Add
BLAST
Alternative sequencei117 – 564448Missing in isoform B.
VSP_030266Add
BLAST
Alternative sequencei133 – 565433Missing in isoform C.
VSP_030267Add
BLAST
Alternative sequencei1315 – 13151S → SNKDRLFSFNEDVFGCGFS in isoform B.
VSP_028732
Alternative sequencei1445 – 145713KQGTQ…GWLDD → QERFMHLEKQTRI in isoform B.
VSP_028733Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti32 – 321A → V in BAA04135. 1 Publication
Sequence conflicti208 – 2081S → T in BAA04135. 1 Publication
Sequence conflicti438 – 4381N → K in BAA04135. 1 Publication
Sequence conflicti565 – 5651R → RR in BAA04135. 1 Publication
Sequence conflicti667 – 6671K → N in BAA04135. 1 Publication
Sequence conflicti679 – 6846Missing in BAA04135. 1 Publication
Sequence conflicti778 – 7781G → GGG in BAA04135. 1 Publication
Sequence conflicti924 – 9241L → M in AAQ22428. 1 Publication
Sequence conflicti983 – 9831E → G in AAQ22428. 1 Publication
Sequence conflicti1128 – 11281I → N in AAQ22428. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17315 mRNA. Translation: BAA04135.1.
AE014298 Genomic DNA. Translation: AAF46430.2.
AE014298 Genomic DNA. Translation: ABW09364.1.
AE014298 Genomic DNA. Translation: ABW09365.1.
AY075349 mRNA. Translation: AAL68208.1.
BT009959 mRNA. Translation: AAQ22428.1. Sequence problems.
PIRiT13709.
RefSeqiNP_001096916.1. NM_001103446.2. [Q09103-3]
NP_001096917.1. NM_001103447.3. [Q09103-2]
NP_511092.2. NM_078537.3. [Q09103-1]
UniGeneiDm.5516.

Genome annotation databases

EnsemblMetazoaiFBtr0302660; FBpp0291800; FBgn0261549. [Q09103-1]
GeneIDi31826.
KEGGidme:Dmel_CG42667.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17315 mRNA. Translation: BAA04135.1 .
AE014298 Genomic DNA. Translation: AAF46430.2 .
AE014298 Genomic DNA. Translation: ABW09364.1 .
AE014298 Genomic DNA. Translation: ABW09365.1 .
AY075349 mRNA. Translation: AAL68208.1 .
BT009959 mRNA. Translation: AAQ22428.1 . Sequence problems.
PIRi T13709.
RefSeqi NP_001096916.1. NM_001103446.2. [Q09103-3 ]
NP_001096917.1. NM_001103447.3. [Q09103-2 ]
NP_511092.2. NM_078537.3. [Q09103-1 ]
UniGenei Dm.5516.

3D structure databases

ProteinModelPortali Q09103.
SMRi Q09103. Positions 1275-1443.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 58282. 12 interactions.

Proteomic databases

PaxDbi Q09103.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0302660 ; FBpp0291800 ; FBgn0261549 . [Q09103-1 ]
GeneIDi 31826.
KEGGi dme:Dmel_CG42667.

Organism-specific databases

CTDi 31826.
FlyBasei FBgn0261549. rdgA.

Phylogenomic databases

eggNOGi COG0666.
GeneTreei ENSGT00740000115137.
InParanoidi Q09103.
KOi K00901.
OMAi RRTICCM.
OrthoDBi EOG76HQ0Q.
PhylomeDBi Q09103.

Enzyme and pathway databases

SABIO-RK Q09103.
SignaLinki Q09103.

Miscellaneous databases

ChiTaRSi rdgA. drosophila.
GenomeRNAii 31826.
NextBioi 775516.
PROi Q09103.

Gene expression databases

Bgeei Q09103.

Family and domain databases

Gene3Di 1.25.40.20. 1 hit.
InterProi IPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
[Graphical view ]
Pfami PF00023. Ank. 2 hits.
PF00130. C1_1. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view ]
SMARTi SM00248. ANK. 4 hits.
SM00109. C1. 1 hit.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEi PS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 2 hits.
PS50146. DAGK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Drosophila retinal degeneration A gene encodes an eye-specific diacylglycerol kinase with cysteine-rich zinc-finger motifs and ankyrin repeats."
    Masai I., Okazaki A., Hosoya T., Hotta Y.
    Proc. Natl. Acad. Sci. U.S.A. 90:11157-11161(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    Strain: Canton-S.
    Tissue: Head.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
    Strain: Berkeley.
    Tissue: Head.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
    Strain: Berkeley.
    Tissue: Head.

Entry informationi

Entry nameiDGK2_DROME
AccessioniPrimary (citable) accession number: Q09103
Secondary accession number(s): A8JV38
, A8JV40, Q0KHU7, Q7YU71, Q8SY47, Q9W3A4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: June 21, 2005
Last modified: July 9, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi