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Protein

Locomotion-related protein Hikaru genki

Gene

hig

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the formation of functional neural circuits from the early stages of synapse formation. Has a role in the development of CNS functions involved in locomotor activity.3 Publications

GO - Biological processi

  • synaptic target recognition Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Locomotion-related protein Hikaru genki
Gene namesi
Name:hig
ORF Names:CG2040
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0010114. hig.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: FlyBase
  • neuronal cell body Source: FlyBase
  • synaptic cleft Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Flies are unable to jump or fly and walk slowly with an unstable gait. Occasionally they exhibit body and wing tremors while standing or walking.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Sequence analysisAdd
BLAST
Chaini31 – 958928Locomotion-related protein Hikaru genkiPRO_0000014774Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi376 – 3761N-linked (GlcNAc...)1 Publication
Glycosylationi525 – 5251N-linked (GlcNAc...)Sequence analysis
Disulfide bondi569 ↔ 597By similarity
Glycosylationi605 – 6051N-linked (GlcNAc...)Sequence analysis
Glycosylationi620 – 6201N-linked (GlcNAc...)Sequence analysis
Disulfide bondi643 ↔ 695By similarity
Disulfide bondi714 ↔ 755By similarity
Disulfide bondi741 ↔ 768By similarity
Glycosylationi752 – 7521N-linked (GlcNAc...)Sequence analysis
Disulfide bondi773 ↔ 814By similarity
Glycosylationi789 – 7891N-linked (GlcNAc...)Sequence analysis
Disulfide bondi800 ↔ 827By similarity
Disulfide bondi832 ↔ 877By similarity
Disulfide bondi863 ↔ 890By similarity
Disulfide bondi894 ↔ 939By similarity
Disulfide bondi922 ↔ 952By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ09101.

Expressioni

Tissue specificityi

Expressed in PCC neurons and neuroblasts in the procephalic neurogenic region in the central nervous system. Expressed in nerves running along the surface of the ventral ganglion and that extend to the peripheral tissues. Also expressed in motor nerves and boutons of certain muscles, in particular of muscle 8 in the A2 and posterior abdominal segments of young adult flies. In the brain, expressed in both the neuropiles and cortical regions except the retinular cells and laminar neuropile.2 Publications

Developmental stagei

Most abundant during and/or after neuronal differentiation and during cell specification or axogenesis. Required during pupation for normal motor function development.1 Publication

Gene expression databases

BgeeiQ09101.
ExpressionAtlasiQ09101. differential.
GenevisibleiQ09101. DM.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Dmel_CG34114Q0KI852EBI-172012,EBI-6884983

Protein-protein interaction databases

BioGridi61786. 1 interaction.
IntActiQ09101. 4 interactions.
MINTiMINT-875412.

Structurei

3D structure databases

ProteinModelPortaliQ09101.
SMRiQ09101. Positions 733-953.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini527 – 59973Sushi 1PROSITE-ProRule annotationAdd
BLAST
Domaini612 – 70897Ig-like C2-typeAdd
BLAST
Domaini712 – 77059Sushi 2PROSITE-ProRule annotationAdd
BLAST
Domaini771 – 82959Sushi 3PROSITE-ProRule annotationAdd
BLAST
Domaini830 – 89263Sushi 4PROSITE-ProRule annotationAdd
BLAST
Domaini892 – 95463Sushi 5PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi318 – 3203Cell attachment site

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi165 – 1684Poly-Arg
Compositional biasi232 – 29059Lys-richAdd
BLAST
Compositional biasi334 – 3396Poly-Arg
Compositional biasi441 – 4488Poly-Glu

Sequence similaritiesi

Contains 5 Sushi (CCP/SCR) domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Sushi

Phylogenomic databases

eggNOGiENOG410IS3E. Eukaryota.
ENOG410XSAY. LUCA.
GeneTreeiENSGT00760000118803.
InParanoidiQ09101.
OMAiYYVHIAD.
OrthoDBiEOG7QVM2Z.
PhylomeDBiQ09101.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR000436. Sushi_SCR_CCP_dom.
[Graphical view]
PfamiPF00084. Sushi. 4 hits.
[Graphical view]
SMARTiSM00032. CCP. 5 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF57535. SSF57535. 4 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS50923. SUSHI. 5 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 3 (identifier: Q09101-1) [UniParc]FASTAAdd to basket

Also known as: A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MWSRQRMRHK PLWALISLTV LLLVLDKSNA NTEPVETSTT SEPDASPGCR
60 70 80 90 100
APDVRFTIVK PEATTEQPLA KFETTQIYLP EDFTTADVEF VDSVPRHPNE
110 120 130 140 150
NHAAVINPYS LDLGDDHLHV GDAAASLVGD DDLGDEDEDH HGDPEDKRLN
160 170 180 190 200
ANRKQGKRRR AGSGRRRRIE NENGQTGRGR GSRYKRHAIL HDTEASPETD
210 220 230 240 250
RWAGSKLAAE GDVYYVHIAD ILKSREPNRE LKSKLHKLKM KARLNKCLAE
260 270 280 290 300
GGKEKCTRLL KKKPKKKVVE KEQTLKKEKK FPKEEQSKEK VPENGQTPKE
310 320 330 340 350
DELELDHPET AAAHHRRRGD SHAAELDQRD RSPRWRRRRS TEFKGDLGQL
360 370 380 390 400
PPESGIGPEP EPLADQDLKD LQQYGNQSSS ARVALLWQRV KRKSGKTTGA
410 420 430 440 450
LSRPKGGGDS SSKTTSRKDK GIYDEEAGYT PIHPDDPEFD EEEEEDEEVD
460 470 480 490 500
ILQQFTEVSE IRFPGEIGPM GDRRLCKIRC VKGKWVGPLC ATNEEDDNGN
510 520 530 540 550
VKFQPLYKSC HVNRIPSHLL LSYRNISVTP IPPNRGWRKT RLSKSTLLSN
560 570 580 590 600
TEINVGWDLP HGHSLQARCQ ELGIYKLLGE SRVLCSNGLW APRMPSCVPT
610 620 630 640 650
TVLTNYSEDS APSIRIKIFN GSHSFEPSGV MAVPPHSTVL MDCMYPRVRG
660 670 680 690 700
TPEWSWTSWY MQYSTGWSPA QEEKAVRYRL SIKNIENNDS GTFTCTSPRG
710 720 730 740 750
LTNSIAVVVA TSTCPQLTEP LAPLKLRLEG NKLGQRAHYE CPEGFRLDGA
760 770 780 790 800
WNATCLASGN WSSPTPTCHA IQCPRLELDD PHLSLIELNT SAWGRAVFKC
810 820 830 840 850
QWGFKLTGPA QLDCEPSGVW SGPVPRCKVI QCVMPVAPLN GRIGGTSLSQ
860 870 880 890 900
RRLTVGALVT FSCNDGHSLV GESSIICTEN GQWSHSPPFC KSQCPYPGDP
910 920 930 940 950
PNGLIAPLKF NYDAGDYLSV QCRPGFVQSY EGPPERPKCQ PDGRWSGPMP

KCKSYEEV
Length:958
Mass (Da):107,028
Last modified:November 8, 2005 - v2
Checksum:i7B2A08477672E674
GO
Isoform 1 (identifier: Q09101-2) [UniParc]FASTAAdd to basket

Also known as: D

The sequence of this isoform differs from the canonical sequence as follows:
     892-958: Missing.

Show »
Length:891
Mass (Da):99,596
Checksum:i4E82C3F43582C16A
GO
Isoform 2 (identifier: Q09101-3) [UniParc]FASTAAdd to basket

Also known as: C

The sequence of this isoform differs from the canonical sequence as follows:
     529-553: Missing.
     892-958: Missing.

Show »
Length:866
Mass (Da):96,748
Checksum:iF0FFE6BC206088ED
GO
Isoform 4 (identifier: Q09101-4) [UniParc]FASTAAdd to basket

Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     529-553: Missing.

Show »
Length:933
Mass (Da):104,179
Checksum:i9C0B696854C6C9FA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti81 – 811E → D in BAA02984 (PubMed:8461133).Curated
Sequence conflicti81 – 811E → D in BAA02985 (PubMed:8461133).Curated
Sequence conflicti81 – 811E → D in BAA02986 (PubMed:8461133).Curated
Sequence conflicti81 – 811E → D in BAA02987 (PubMed:8461133).Curated
Sequence conflicti292 – 2921P → L in BAA02984 (PubMed:8461133).Curated
Sequence conflicti292 – 2921P → L in BAA02985 (PubMed:8461133).Curated
Sequence conflicti292 – 2921P → L in BAA02986 (PubMed:8461133).Curated
Sequence conflicti292 – 2921P → L in BAA02987 (PubMed:8461133).Curated
Sequence conflicti367 – 3671D → N in BAA02984 (PubMed:8461133).Curated
Sequence conflicti367 – 3671D → N in BAA02985 (PubMed:8461133).Curated
Sequence conflicti367 – 3671D → N in BAA02986 (PubMed:8461133).Curated
Sequence conflicti367 – 3671D → N in BAA02987 (PubMed:8461133).Curated
Sequence conflicti396 – 3961K → R (PubMed:8461133).Curated
Sequence conflicti398 – 3981T → A (PubMed:8461133).Curated
Sequence conflicti716 – 7161Q → R in BAA02984 (PubMed:8461133).Curated
Sequence conflicti716 – 7161Q → R in BAA02985 (PubMed:8461133).Curated
Sequence conflicti716 – 7161Q → R in BAA02986 (PubMed:8461133).Curated
Sequence conflicti716 – 7161Q → R in BAA02987 (PubMed:8461133).Curated
Sequence conflicti760 – 7601N → S in BAA02984 (PubMed:8461133).Curated
Sequence conflicti760 – 7601N → S in BAA02985 (PubMed:8461133).Curated
Sequence conflicti760 – 7601N → S in BAA02986 (PubMed:8461133).Curated
Sequence conflicti760 – 7601N → S in BAA02987 (PubMed:8461133).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei529 – 55325Missing in isoform 2 and isoform 4. 2 PublicationsVSP_002512Add
BLAST
Alternative sequencei892 – 95867Missing in isoform 1 and isoform 2. 2 PublicationsVSP_002513Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13884 mRNA. Translation: BAA02984.1.
D13885 mRNA. Translation: BAA02985.1.
D13886 mRNA. Translation: BAA02986.1.
D13887 mRNA. Translation: BAA02987.1.
AE013599 Genomic DNA. Translation: AAF58960.1.
AE013599 Genomic DNA. Translation: AAG22296.1.
AE013599 Genomic DNA. Translation: AAG22297.1.
AE013599 Genomic DNA. Translation: AAS64892.2.
AY069188 mRNA. Translation: AAL39333.1.
BT001427 mRNA. Translation: AAN71182.1.
RefSeqiNP_001014514.1. NM_001014514.2. [Q09101-2]
NP_525110.2. NM_080371.3. [Q09101-3]
NP_724772.1. NM_165666.2. [Q09101-4]
NP_724773.1. NM_165667.2. [Q09101-1]
UniGeneiDm.2241.

Genome annotation databases

EnsemblMetazoaiFBtr0088607; FBpp0087688; FBgn0010114. [Q09101-1]
GeneIDi35949.
KEGGidme:Dmel_CG2040.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13884 mRNA. Translation: BAA02984.1.
D13885 mRNA. Translation: BAA02985.1.
D13886 mRNA. Translation: BAA02986.1.
D13887 mRNA. Translation: BAA02987.1.
AE013599 Genomic DNA. Translation: AAF58960.1.
AE013599 Genomic DNA. Translation: AAG22296.1.
AE013599 Genomic DNA. Translation: AAG22297.1.
AE013599 Genomic DNA. Translation: AAS64892.2.
AY069188 mRNA. Translation: AAL39333.1.
BT001427 mRNA. Translation: AAN71182.1.
RefSeqiNP_001014514.1. NM_001014514.2. [Q09101-2]
NP_525110.2. NM_080371.3. [Q09101-3]
NP_724772.1. NM_165666.2. [Q09101-4]
NP_724773.1. NM_165667.2. [Q09101-1]
UniGeneiDm.2241.

3D structure databases

ProteinModelPortaliQ09101.
SMRiQ09101. Positions 733-953.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi61786. 1 interaction.
IntActiQ09101. 4 interactions.
MINTiMINT-875412.

Proteomic databases

PaxDbiQ09101.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088607; FBpp0087688; FBgn0010114. [Q09101-1]
GeneIDi35949.
KEGGidme:Dmel_CG2040.

Organism-specific databases

CTDi35949.
FlyBaseiFBgn0010114. hig.

Phylogenomic databases

eggNOGiENOG410IS3E. Eukaryota.
ENOG410XSAY. LUCA.
GeneTreeiENSGT00760000118803.
InParanoidiQ09101.
OMAiYYVHIAD.
OrthoDBiEOG7QVM2Z.
PhylomeDBiQ09101.

Miscellaneous databases

ChiTaRSihig. fly.
GenomeRNAii35949.
PROiQ09101.

Gene expression databases

BgeeiQ09101.
ExpressionAtlasiQ09101. differential.
GenevisibleiQ09101. DM.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR000436. Sushi_SCR_CCP_dom.
[Graphical view]
PfamiPF00084. Sushi. 4 hits.
[Graphical view]
SMARTiSM00032. CCP. 5 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
SSF57535. SSF57535. 4 hits.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS50923. SUSHI. 5 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Hikaru genki, a CNS-specific gene identified by abnormal locomotion in Drosophila, encodes a novel type of protein."
    Hoshino M., Matsuzaki F., Nabeshima Y., Hama C.
    Neuron 10:395-407(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), FUNCTION, DISRUPTION PHENOTYPE.
    Tissue: Head.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 429-958 (ISOFORM 1).
    Strain: Berkeley.
    Tissue: Head.
  5. "Hikaru genki protein is secreted into synaptic clefts from an early stage of synapse formation in Drosophila."
    Hoshino M., Suzuki E., Nabeshima Y., Hama C.
    Development 122:589-597(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  6. "Neural expression of hikaru genki protein during embryonic and larval development of Drosophila melanogaster."
    Hoshino M., Suzuki E., Miyake T., Sone M., Komatsu A., Nabeshima Y., Hama C.
    Dev. Genes Evol. 209:1-9(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  7. "Identification of N-glycosylated proteins from the central nervous system of Drosophila melanogaster."
    Koles K., Lim J.-M., Aoki K., Porterfield M., Tiemeyer M., Wells L., Panin V.
    Glycobiology 17:1388-1403(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-376, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Oregon-R.
    Tissue: Head.

Entry informationi

Entry nameiHIG_DROME
AccessioniPrimary (citable) accession number: Q09101
Secondary accession number(s): Q7KQU0
, Q8IH50, Q9I7E3, Q9I7E4, Q9V560
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 8, 2005
Last modified: July 6, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.