Reviewed, UniProtKB/Swiss-Prot Q09067 (UREH_HELPY)

Names and origin

Protein names

Recommended name:
    Urease accessory protein ureH

Gene names

Name:	ureH
Ordered Locus Names:	HP_0067

Organism

Helicobacter pylori (Campylobacter pylori) [Complete proteome] [HAMAP]

Taxonomic identifier

210 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required for maturation of urease via the functional incorporation of the urease nickel metallocenter By similarity.
Subunit structure	UreH, ureF and ureG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of ureC. The ureE protein probably delivers the nickel By similarity.
Subcellular location	Cytoplasm By similarity.
Disruption phenotype	Cells do not express urease. Ref.1
Sequence similarities	Belongs to the ureD family.

Ontologies

Keywords
Biological process	Nickel insertion Virulence
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	Urease accessory protein ureH HAMAP MF_01384		PRO_0000067624
Experimental info
	Sequence conflict	57 – 59	3	MMR → LMK in AAA25026. Ref.1
	Sequence conflict	214 – 215	2	EC → GL in AAA25026. Ref.1
	Sequence conflict	229	1	T → I in AAA25026. Ref.1
	Sequence conflict	236	1	V → L in AAA25026. Ref.1
	Sequence conflict	256 – 257	2	LV → FI in AAA25026. Ref.1
	Sequence conflict	261 – 263	3	TTQ → ITP in AAA25026. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q09067-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 8F16568C7DF9B162	FASTA	265	29,722
	10 20 30 40 50 60 MNTYAQESKL RLKTKIGADG RCVIEDNFFT PPFKLMAPFY PKDDLAEIML LAVSPGMMRG 70 80 90 100 110 120 DAQDVQLNIG PNCKLRITSQ SFEKIHNTED GFASRDMHIV VGENAFLDFA PFPLIPFENA 130 140 150 160 170 180 HFKGNTTISL RSSSQLLYSE IIVAGRVARN ELFKFNRLHT KISILQDEKP IYYDNTILDP 190 200 210 220 230 240 KTTDLNNMCM FDGYTHYLNL VLVNCPIELS GVRECIEESE GVDGAVSETA SSHLCVKALA 250 260 KGSEPLLHLR EKIARLVTQT TTQKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of Helicobacter pylori urease genes in Escherichia coli grown under nitrogen-limiting conditions." Cussac V., Ferrero R.L., Labigne A. J. Bacteriol. 174:2466-2473(1992) [PubMed: 1313413] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE. Strain: 85P.
[2]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.
[3]	"Bacterial factors that mediate colonization of the stomach and virulence of Helicobacter pylori." Clyne M., Dolan B., Reeves E.P. FEMS Microbiol. Lett. 268:135-143(2007) [PubMed: 17313591] [Abstract] Cited for: REVIEW ON VIRULENCE OF H.PYLORI.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M84338 Genomic DNA. Translation: AAA25026.1. AE000511 Genomic DNA. Translation: AAD07130.1.
PIR	C64528.
RefSeq	NP_206867.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:3135N.
Genome annotation databases
GeneID	899986.
GenomeReviews	Gene locus HP_0067 in contig AE000511_GR.
KEGG	hpy:HP0067.
NMPDR	fig|85962.1.peg.65.
TIGR	HP_0067.
Phylogenomic databases
HOGENOM	Q09067.
OMA	CKLRITS
Family and domain databases
HAMAP	MF_01384. [Tree]
InterPro	IPR002669. UreD. [Graphical view]
Pfam	PF01774. UreD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UREH_HELPY

Accession

Primary (citable) accession number: Q09067

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1997
Last modified:	November 3, 2009
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)