Q09065 (UREF_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urease accessory protein UreF

Gene names

Name:	ureF
Ordered Locus Names:	HP_0069

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for maturation of urease via the functional incorporation of the urease nickel metallocenter By similarity. HAMAP MF_01385
Subunit structure	UreH, UreF and UreG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of UreC. The UreE protein probably delivers the nickel By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_01385.
Disruption phenotype	Cells do not express urease. Ref.1
Sequence similarities	Belongs to the UreF family.

Ontologies

Keywords
Biological process	Nickel insertion Virulence
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 254

254

Urease accessory protein UreF HAMAP MF_01385

PRO_0000067648

Experimental info

Sequence conflict

10

1

T → I in AAA25024. Ref.1

Sequence conflict

17

1

P → L in AAA25024. Ref.1

Sequence conflict

25

1

N → S in AAA25024. Ref.1

Sequence conflict

55 – 60

6

ETYIQQ → LARNLHPA in AAA25024. Ref.1

Sequence conflict

71

1

E → K in AAA25024. Ref.1

Sequence conflict

103

1

K → R in AAA25024. Ref.1

Sequence conflict

110 – 112

3

VIM → IIT in AAA25024. Ref.1

Sequence conflict

143 – 145

3

MGE → IGA in AAA25024. Ref.1

Sequence conflict

153 – 155

3

KTK → QTE in AAA25024. Ref.1

Sequence conflict

232

1

T → A in AAA25024. Ref.1

Secondary structure

1

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254

Helix Strand Turn

Details...

Helix

31 – 39

9

Helix

55 – 59

5

Helix

66 – 77

12

Helix

80 – 83

4

Helix

85 – 97

13

Helix

101 – 104

4

Helix

108 – 114

7

Helix

118 – 126

9

Helix

130 – 137

8

Helix

146 – 153

8

Helix

159 – 162

4

Helix

166 – 169

4

Helix

174 – 196

23

Helix

206 – 210

5

Helix

212 – 224

13

Helix

227 – 229

3

Sequences

	Sequence		Length	Mass (Da)
	Q09065 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 6209F547A96FE028	FASTA	254	28,619
	10 20 30 40 50 60 MDKGKSVKST EKSVGMPPKT PKTDNNAHVD NEFLILQVND AVFPIGSYTH SFGLETYIQQ 70 80 90 100 110 120 KKVTNKESAL EYLKANLSSQ FLYTEMLSLK LTYESALQQD LKKILGVEEV IMLSTSPMEL 130 140 150 160 170 180 RLANQKLGNR FIKTLQAMNE LDMGEFFNAY AQKTKDPTHA TSYGVFAASL GIELKKALRH 190 200 210 220 230 240 YLYAQTSNMV INCVKSVPLS QNDGQKILLS LQSPFNQLIE KTLELDESHL CTASVQNDIK 250 AMQHESLYSR LYMS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression of Helicobacter pylori urease genes in Escherichia coli grown under nitrogen-limiting conditions." Cussac V., Ferrero R.L., Labigne A. J. Bacteriol. 174:2466-2473(1992) [PubMed: 1313413] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DISRUPTION PHENOTYPE. Strain: 85P.
[2]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed: 9252185] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.
[3]	"Bacterial factors that mediate colonization of the stomach and virulence of Helicobacter pylori." Clyne M., Dolan B., Reeves E.P. FEMS Microbiol. Lett. 268:135-143(2007) [PubMed: 17313591] [Abstract] Cited for: REVIEW ON VIRULENCE OF H.PYLORI.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M84338 Genomic DNA. Translation: AAA25024.1.
AE000511 Genomic DNA. Translation: AAD07132.1.

PIR

E64528.

RefSeq

NP_206869.1. NC_000915.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2WGL	X-ray	2.00	A/B/C	1-254	[»]
3CXN	X-ray	1.55	A/B/C	1-254	[»]
3O1Q	X-ray	1.85	A/B/C	1-254	[»]
3SF5	X-ray	2.50	A/C	1-254	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-3134N.

MINT

MINT-184930.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

900170.

GenomeReviews

Gene locus HP_0069 in contig AE000511_GR.

KEGG

hpy:HP0069.

NMPDR

fig|85962.1.peg.67.

PATRIC

20591339. VBIHelPyl33062_0071.

TIGR

HP_0069.

Phylogenomic databases

HOGENOM

HBG296674.

OMA

STSPMEL.

ProtClustDB

CLSK495847.

Family and domain databases

HAMAP

MF_01385. UreF.
[Tree]

InterPro

IPR002639. UreF.
[Graphical view]

KO

K03188.

Pfam

PF01730. UreF. 1 hit.
[Graphical view]

PIRSF

PIRSF009467. Ureas_acces_UreF. 1 hit.

ProtoNet

Search...

Entry information

Entry name

UREF_HELPY

Accession

Primary (citable) accession number: Q09065

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program