Q09063 (URED_ENTAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urease accessory protein UreD

Gene names

Name:

ureD

Organism

Enterobacter aerogenes (Aerobacter aerogenes)

Taxonomic identifier

548 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Enterobacter

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Necessary for the functional incorporation of the urease nickel metallocenter. HAMAP MF_01384
Subunit structure	UreD, UreF and UreG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of UreC. The complex may form in the order UreABCD, UreABCDF, UreABCDFG. The UreE protein probably delivers the nickel in a GTPase-dependent fashion.
Subcellular location	Cytoplasm By similarity HAMAP MF_01384.
Sequence similarities	Belongs to the UreD family.

Ontologies

Keywords
Biological process	Nickel insertion
Cellular component	Cytoplasm
Molecular function	Chaperone
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	Urease accessory protein UreD HAMAP MF_01384		PRO_0000067611

Sequences

	Sequence		Length	Mass (Da)
	Q09063 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: F87D0647DFB7FE91	FASTA	270	29,840
	10 20 30 40 50 60 MLPPLKKGWQ ATLDLRFHQA GGKTVLASAQ HVGPLTVQRP FYPEEETCHL YLLHPPGGIV 70 80 90 100 110 120 GGDELTISAH LAPGCHTLIT MPGASKFYRS SGAQALVRQQ LTLAPQATLE WLPQDAIFFP 130 140 150 160 170 180 GANARLFTTF HLCASSRLLA WDLLCLGRPV IGETFSHGTL SNRLEVWVDN EPLLVERLHL 190 200 210 220 230 240 QEGELSSIAE RPWVGTLLCY PATDALLDGV RDALAPLGLY AGASLTDRLL TVRFLSDDNL 250 260 270 ICQRVMRDVW QFLRPHLTGK SPVLPRIWLT

References

[1]	"Klebsiella aerogenes urease gene cluster: sequence of ureD and demonstration that four accessory genes (ureD, ureE, ureF, and ureG) are involved in nickel metallocenter biosynthesis." Lee M.H., Mulrooney S.B., Renner M.J., Markowicz Y., Hausinger R.P. J. Bacteriol. 174:4324-4330(1992) [PubMed: 1624427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"In vitro activation of urease apoprotein and role of UreD as a chaperone required for nickel metallocenter assembly." Park I.-S., Carr M.B., Hausinger R.P. Proc. Natl. Acad. Sci. U.S.A. 91:3233-3237(1994) [PubMed: 7909161] [Abstract] Cited for: INTERACTION WITH UREASE APOPROTEIN, POSSIBLE CHAPERONE ACTIVITY IN NICKEL METALLOCENTER ASSEMBLY.
[3]	"Evidence for the presence of urease apoprotein complexes containing UreD, UreF, and UreG in cells that are competent for in vivo enzyme activation." Park I.-S., Hausinger R.P. J. Bacteriol. 177:1947-1951(1995) [PubMed: 7721685] [Abstract] Cited for: CATALYTIC ACTIVITY, INTERACTION WITH UREA; UREB; UREC; UREF AND UREG.
[4]	"GTP-dependent activation of urease apoprotein in complex with the UreD, UreF, and UreG accessory proteins." Soriano A., Hausinger R.P. Proc. Natl. Acad. Sci. U.S.A. 96:11140-11144(1999) [PubMed: 10500143] [Abstract] Cited for: COMPLEX FORMATION, INTERACTION WITH UREA; UREB; UREC; UREF AND UREG.
[5]	"Chemical cross-linking and mass spectrometric identification of sites of interaction for UreD, UreF, and urease." Chang Z., Kuchar J., Hausinger R.P. J. Biol. Chem. 279:15305-15313(2004) [PubMed: 14749331] [Abstract] Cited for: INTERACTION WITH UREB AND UREC, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M55391 Genomic DNA. Translation: AAA25148.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01384. UreD. [Tree]
InterPro	IPR002669. UreD. [Graphical view]
Pfam	PF01774. UreD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URED_ENTAE

Accession

Primary (citable) accession number: Q09063

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 16, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program