Reviewed, UniProtKB/Swiss-Prot Q09023 (CHI2_BRANA)

Names and origin

Protein names	Recommended name:     Endochitinase CH25     EC=3.2.1.14
Organism	Brassica napus (Rape)
Taxonomic identifier	3708 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassica

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Tissue specificity	High expression in roots, moderate in floral tissues and low in stems and leaves. Ref.2
Induction	In roots by wounding and ethephon. Ref.2
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Polysaccharide degradation
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 322	302	Endochitinase CH25		PRO_0000005290
Regions
	Domain	21 – 62	42	Chitin-binding type-1
Amino acid modifications
	Disulfide bond	23 ↔ 38		By similarity
	Disulfide bond	32 ↔ 44		By similarity
	Disulfide bond	37 ↔ 51		By similarity
	Disulfide bond	56 ↔ 60		By similarity
	Disulfide bond	92 ↔ 154		By similarity
	Disulfide bond	166 ↔ 174		By similarity
	Disulfide bond	273 ↔ 305		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q09023-1 [UniParc]. Last modified October 1, 1994. Version 1. Checksum: E62EE4B17211DBCD	FASTA	322	34,816
	10 20 30 40 50 60 MKSCLLLFLI FSFLLSFSLA EQCGRQAGGA LCPNGLCCSE FGWCGDTEAY CKQPGCQSQC 70 80 90 100 110 120 GGTPPGPTGD LSGIISRSQF DDMLKHRNDN ACPARGFYTY DAFINAAKSF PGFGTTGDTA 130 140 150 160 170 180 TRKKEIAAFF GQTSHETTGG WATAPDGPYS WGYCFKQEQN PSSNYCSPSA EWPCASGKSY 190 200 210 220 230 240 YGRGPMQLSW NYNYGQCGRA IGSDLLNNPD LVSNDPVIAF KAAIWFWMTP QSPKPSCHAV 250 260 270 280 290 300 IVGQWQPSDA DRAAGRVPGY GVITNIINGG LECGRGQDAR VADRIGFYQR YCNILGVNPG 310 320 GNLDCYNQRS FASVNFFLDA AI

References

[1]	"Nucleotide sequence of a Brassica napus endochitinase gene." Hamel F., Bellemare G. Plant Physiol. 101:1403-1403(1993) [PubMed: 8310072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Characterization of a class I chitinase gene and of wound-inducible, root and flower-specific chitinase expression in Brassica napus." Hamel F., Bellemare G. Biochim. Biophys. Acta 1263:212-220(1995) [PubMed: 7548207] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION. Strain: cv. Westar.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M95835 Genomic DNA. Translation: AAA32986.1.
PIR	S59953.
3D structure databases
SMR	Q09023. Positions 21-311.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Enzyme and pathway databases
BRENDA	3.2.1.14. 393.
Family and domain databases
InterPro	IPR018371. Chitin-binding_1_CS. IPR001002. Chitin_bd_1. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. [Graphical view]
Gene3D	G3DSA:3.30.60.10. Chitin_bd_1. 1 hit.
PANTHER	PTHR22595. Glyco_hydro_19_cat. 1 hit.
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_bd_1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI2_BRANA

Accession

Primary (citable) accession number: Q09023

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1994
Last sequence update:	October 1, 1994
Last modified:	February 9, 2010
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)