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Protein

Endochitinase CH25

Gene
N/A
Organism
Brassica napus (Rape)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Chitin degradation, Polysaccharide degradation
LigandChitin-binding

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Endochitinase CH25 (EC:3.2.1.14)
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000000529021 – 322Endochitinase CH25Add BLAST302

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi23 ↔ 38PROSITE-ProRule annotation
Disulfide bondi32 ↔ 44PROSITE-ProRule annotation
Disulfide bondi37 ↔ 51PROSITE-ProRule annotation
Disulfide bondi56 ↔ 60PROSITE-ProRule annotation
Disulfide bondi92 ↔ 154PROSITE-ProRule annotation
Disulfide bondi166 ↔ 174PROSITE-ProRule annotation
Disulfide bondi273 ↔ 305PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ09023.

Expressioni

Tissue specificityi

High expression in roots, moderate in floral tissues and low in stems and leaves.1 Publication

Inductioni

In roots by wounding and ethephon.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ09023.
SMRiQ09023.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 62Chitin-binding type-1PROSITE-ProRule annotationAdd BLAST42

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

KOiK20547.

Family and domain databases

CDDicd00325. chitinase_glyco_hydro_19. 1 hit.
Gene3Di3.30.60.10. 1 hit.
InterProiView protein in InterPro
IPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR036861. Endochitinase-like_sf.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom_sf.
PfamiView protein in Pfam
PF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
PIRSFiPIRSF001060. Endochitinase. 1 hit.
PRINTSiPR00451. CHITINBINDNG.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD000609. Chitin_bd_1. 1 hit.
SMARTiView protein in SMART
SM00270. ChtBD1. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiView protein in PROSITE
PS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q09023-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSCLLLFLI FSFLLSFSLA EQCGRQAGGA LCPNGLCCSE FGWCGDTEAY
60 70 80 90 100
CKQPGCQSQC GGTPPGPTGD LSGIISRSQF DDMLKHRNDN ACPARGFYTY
110 120 130 140 150
DAFINAAKSF PGFGTTGDTA TRKKEIAAFF GQTSHETTGG WATAPDGPYS
160 170 180 190 200
WGYCFKQEQN PSSNYCSPSA EWPCASGKSY YGRGPMQLSW NYNYGQCGRA
210 220 230 240 250
IGSDLLNNPD LVSNDPVIAF KAAIWFWMTP QSPKPSCHAV IVGQWQPSDA
260 270 280 290 300
DRAAGRVPGY GVITNIINGG LECGRGQDAR VADRIGFYQR YCNILGVNPG
310 320
GNLDCYNQRS FASVNFFLDA AI
Length:322
Mass (Da):34,816
Last modified:October 1, 1994 - v1
Checksum:iE62EE4B17211DBCD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95835 Genomic DNA. Translation: AAA32986.1.
PIRiS59953.
RefSeqiXP_013731720.1. XM_013876266.1.

Genome annotation databases

EnsemblPlantsiCDY42151; CDY42151; GSBRNA2T00074333001.
GeneIDi106435387.
GrameneiCDY42151; CDY42151; GSBRNA2T00074333001.
KEGGibna:106435387.

Similar proteinsi

Entry informationi

Entry nameiCHI2_BRANA
AccessioniPrimary (citable) accession number: Q09023
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1994
Last sequence update: October 1, 1994
Last modified: November 22, 2017
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families