Q09014 (NCF1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neutrophil cytosol factor 1
Short name=NCF-1

Alternative name(s):
47 kDa neutrophil oxidase factor
NCF-47K
Neutrophil NADPH oxidase factor 1
p47-phox

Gene names

Name:

Ncf1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Subunit structure	Interacts with NOXA1 By similarity. Interacts with ADAM15 and TRAF4. Interacts with FASLG By similarity.
Subcellular location	Cytoplasm.
Post-translational modification	Phosphorylated by PRKCD; phosphorylation induces activation of NCF1 and NADPH oxidase activity By similarity.
Sequence similarities	Contains 1 PX (phox homology) domain. Contains 2 SH3 domains.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat SH3 domain
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	NADP catabolic process Inferred from sequence alignment PubMed 10647999. Source: MGI cell communication Inferred from electronic annotation. Source: InterPro cell proliferation Inferred from mutant phenotype PubMed 10987289. Source: MGI cellular defense response Inferred from mutant phenotype PubMed 7650482. Source: MGI hydrogen peroxide biosynthetic process Inferred from mutant phenotype PubMed 15258578. Source: MGI inflammatory response Inferred from mutant phenotype PubMed 11867678. Source: MGI leukotriene metabolic process Inferred from mutant phenotype PubMed 11867678. Source: MGI negative regulation of smooth muscle contraction Inferred from mutant phenotype PubMed 17993584. Source: MGI neutrophil mediated killing of fungus Inferred from mutant phenotype PubMed 11907569. Source: MGI neutrophil mediated killing of gram-positive bacterium Inferred from mutant phenotype PubMed 11907569. Source: MGI protein targeting to membrane Inferred from sequence or structural similarity. Source: UniProtKB respiratory burst involved in defense response Inferred from mutant phenotype PubMed 10714686. Source: MGI response to yeast Inferred from mutant phenotype PubMed 10714686PubMed 11907569. Source: MGI superoxide anion generation Inferred from mutant phenotype PubMed 10725280PubMed 11156938. Source: MGI
Cellular_component	cytosol Inferred from sequence alignment PubMed 10647999. Source: MGI
Molecular_function	phosphatidylinositol binding Inferred from sequence or structural similarity. Source: UniProtKB superoxide-generating NADPH oxidase activity Inferred from direct assay Ref.1. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	Neutrophil cytosol factor 1		PRO_0000096763
Regions
	Domain	4 – 125	122	PX
	Domain	162 – 215	54	SH3 1
	Domain	226 – 285	60	SH3 2
	Compositional bias	211 – 254	44	Asp/Glu-rich (highly acidic)
	Compositional bias	292 – 390	99	Arg/Lys-rich (highly basic)
Amino acid modifications
	Modified residue	304	1	Phosphoserine By similarity
	Modified residue	321	1	Phosphoserine Ref.6 Ref.7
	Modified residue	329	1	Phosphoserine By similarity
	Modified residue	346	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	161	1	Y → H in AAA50469. Ref.1
	Sequence conflict	343 – 345	3	GPL → RAA in AAA50469. Ref.1
	Sequence conflict	344	1	P → Q in BAA25649. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q09014 [UniParc]. Last modified July 27, 2011. Version 3. Checksum: C8EFAB953839CE9A	FASTA	390	44,667
	10 20 30 40 50 60 MGDTFIRHIA LLGFEKRFIP SQHYVYMFLV KWQDLSEKVV YRKFTEIYEF HKMLKEMFPI 70 80 90 100 110 120 EAGEIHTENR VIPHLPAPRW FDGQRAAESR QGTLTEYFNG LMGLPVKISR CPHLLDFFKV 130 140 150 160 170 180 RPDDLKLPTD SQAKKPETYL VPKDGKNNVA DITGPIILQT YRAIADYEKS SGTEMTVATG 190 200 210 220 230 240 DVVDVVEKSE SGWWFCQMKT KRGWVPASYL EPLDSPDEAE DPDPNYAGEP YVTIKAYAAV 250 260 270 280 290 300 EEDEMSLSEG EAIEVIHKLL DGWWVVRKGD ITGYFPSMYL QKAGEEITQA QRQIRGRGAP 310 320 330 340 350 360 PRRSTIRNAQ SIHQRSRKRL SQDTYRRNSV RFLQQRRRPG RPGPLSTDGT KDNPSTPRVK 370 380 390 PQPAVPPRPS SDLILHRCTE STKRKLTSAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and functional expression of the mouse homologue of p47phox." Jackson S.H., Malech H.L., Kozak C.A., Lomax K.J., Gallin J.I., Holland S.M. Immunogenetics 39:272-275(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Macrophage.
[2]	"Functional modules and expression of mouse p40(phox) and p67(phox), SH3-domain-containing proteins involved in the phagocyte NADPH oxidase complex." Mizuki K., Kadomatsu K., Hata K., Ito T., Fan Q.-W., Kage Y., Fukumaki Y., Sakaki Y., Takeshige K., Sumimoto H. Eur. J. Biochem. 251:573-582(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leukemia.
[3]	Green E.D. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Bone marrow and Colon.
[5]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-321, MASS SPECTROMETRY. Tissue: Melanoma.
[7]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-321, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L11455 mRNA. Translation: AAA50469.1. AB002663 mRNA. Translation: BAA25649.1. AF267747 Genomic DNA. Translation: AAF90134.1. AK149668 mRNA. Translation: BAE29014.1. AK149732 mRNA. Translation: BAE29053.1. AK152386 mRNA. Translation: BAE31174.1. AK162308 mRNA. Translation: BAE36845.1. AK170462 mRNA. Translation: BAE41812.1. AK171559 mRNA. Translation: BAE42526.1. CH466529 Genomic DNA. Translation: EDL19452.1.
IPI	IPI00120241.
RefSeq	NP_035006.3. NM_010876.3.
UniGene	Mm.425296.
3D structure databases
ProteinModelPortal	Q09014.
SMR	Q09014. Positions 1-128, 156-333, 360-390.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2665N.
IntAct	Q09014. 1 interaction.
PTM databases
PhosphoSite	Q09014.
Proteomic databases
PaxDb	Q09014.
PRIDE	Q09014.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000111275; ENSMUSP00000106906; ENSMUSG00000015950.
GeneID	17969.
KEGG	mmu:17969.
Organism-specific databases
CTD	653361.
MGI	MGI:97283. Ncf1.
Phylogenomic databases
eggNOG	NOG326975.
GeneTree	ENSGT00530000063010.
HOGENOM	HOG000232124.
HOVERGEN	HBG002055.
KO	K08011.
OrthoDB	EOG48PMKD.
Gene expression databases
ArrayExpress	Q09014.
Bgee	Q09014.
CleanEx	MM_NCF1.
Genevestigator	Q09014.
GermOnline	ENSMUSG00000015950. Mus musculus.
Family and domain databases
Gene3D	3.30.1520.10. 1 hit.
InterPro	IPR015039. NADPH_oxidase_p47Phox_C. IPR001655. P47PHOX. IPR001683. Phox. IPR001452. SH3_domain. [Graphical view]
PANTHER	PTHR15706:SF3. PTHR15706:SF3. 1 hit.
Pfam	PF08944. p47_phox_C. 1 hit. PF00787. PX. 1 hit. PF00018. SH3_1. 2 hits. [Graphical view]
PRINTS	PR00498. P47PHOX. PR00452. SH3DOMAIN.
SMART	SM00312. PX. 1 hit. SM00326. SH3. 2 hits. [Graphical view]
SUPFAM	SSF64268. PX. 1 hit. SSF50044. SH3. 2 hits.
PROSITE	PS50195. PX. 1 hit. PS50002. SH3. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	292913.
SOURCE	Search...

Entry information

Entry name

NCF1_MOUSE

Accession

Primary (citable) accession number: Q09014
Secondary accession number(s): O70144, Q3UE58, Q9JI34

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	July 27, 2011
Last modified:	April 3, 2013
This is version 108 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program