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Protein

Neutrophil cytosol factor 1

Gene

Ncf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • cell proliferation Source: MGI
  • cellular defense response Source: MGI
  • cellular response to cadmium ion Source: MGI
  • cellular response to reactive oxygen species Source: MGI
  • defense response to bacterium Source: MGI
  • defense response to fungus Source: MGI
  • defense response to Gram-positive bacterium Source: MGI
  • hydrogen peroxide biosynthetic process Source: MGI
  • inflammatory response Source: MGI
  • leukocyte mediated cytotoxicity Source: MGI
  • leukotriene metabolic process Source: MGI
  • NADP catabolic process Source: MGI
  • neutrophil mediated killing of fungus Source: MGI
  • neutrophil mediated killing of gram-positive bacterium Source: MGI
  • podosome assembly Source: GO_Central
  • positive regulation of epidermal growth factor-activated receptor activity Source: MGI
  • positive regulation of JNK cascade Source: MGI
  • positive regulation of p38MAPK cascade Source: MGI
  • positive regulation of phosphatidylinositol 3-kinase signaling Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • protein targeting to membrane Source: UniProtKB
  • respiratory burst Source: MGI
  • respiratory burst involved in defense response Source: MGI
  • response to bacterium Source: MGI
  • response to yeast Source: MGI
  • superoxide anion generation Source: MGI

Keywordsi

LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-1222556 ROS, RNS production in phagocytes
R-MMU-1236973 Cross-presentation of particulate exogenous antigens (phagosomes)
R-MMU-3299685 Detoxification of Reactive Oxygen Species
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-5668599 RHO GTPases Activate NADPH Oxidases

Names & Taxonomyi

Protein namesi
Recommended name:
Neutrophil cytosol factor 1
Short name:
NCF-1
Alternative name(s):
47 kDa neutrophil oxidase factor
NCF-47K
Neutrophil NADPH oxidase factor 1
p47-phox
Gene namesi
Name:Ncf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:97283 Ncf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000967631 – 390Neutrophil cytosol factor 1Add BLAST390

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei304PhosphoserineBy similarity1
Modified residuei321PhosphoserineBy similarity1
Modified residuei329PhosphoserineBy similarity1
Modified residuei346PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by PRKCD; phosphorylation induces activation of NCF1 and NADPH oxidase activity.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ09014
MaxQBiQ09014
PaxDbiQ09014
PeptideAtlasiQ09014
PRIDEiQ09014

PTM databases

iPTMnetiQ09014
PhosphoSitePlusiQ09014

Expressioni

Gene expression databases

BgeeiENSMUSG00000015950
CleanExiMM_NCF1
ExpressionAtlasiQ09014 baseline and differential
GenevisibleiQ09014 MM

Interactioni

Subunit structurei

Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts (via C-terminus) with NCF2 (via the C-terminal SH3 domain). Interacts with NCF4. Interacts with CYBB. Interacts (via the second SH3 domain) with CYBA. Interacts with NOXA1. Interacts with ADAM15. Interacts with TRAF4. Interacts with FASLG (By similarity). Interacts with PARK7 (via C-terminus); the interaction is enhanced by LPS and modulates NCF1 phosphorylation and membrane translocation (PubMed:26021615).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-2665N
IntActiQ09014, 2 interactors
STRINGi10090.ENSMUSP00000106906

Structurei

3D structure databases

ProteinModelPortaliQ09014
SMRiQ09014
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 125PXPROSITE-ProRule annotationAdd BLAST122
Domaini156 – 215SH3 1PROSITE-ProRule annotationAdd BLAST60
Domaini226 – 285SH3 2PROSITE-ProRule annotationAdd BLAST60

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi211 – 254Asp/Glu-rich (highly acidic)Add BLAST44
Compositional biasi292 – 390Arg/Lys-rich (highly basic)Add BLAST99

Domaini

The PX domain mediates interaction with phosphatidylinositol 3,4-bisphosphate and other anionic phospholipids. In the autoinhibited, unphosphorylated state an intramolecular interaction with the C-terminal SH3 domain precludes phospholipid binding and interaction with CYBA. Phosphorylation disrupts the autoinhibited state (By similarity).By similarity

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiENOG410IRR3 Eukaryota
ENOG410YYZE LUCA
GeneTreeiENSGT00530000063010
HOGENOMiHOG000232124
HOVERGENiHBG002055
InParanoidiQ09014
KOiK08011

Family and domain databases

CDDicd06887 PX_p47phox, 1 hit
cd12021 SH3_p47phox_1, 1 hit
cd12022 SH3_p47phox_2, 1 hit
Gene3Di3.30.1520.10, 1 hit
InterProiView protein in InterPro
IPR015039 NADPH_oxidase_p47Phox_C
IPR035756 NCF1_SH3_1
IPR035757 NCF1_SH3_2
IPR032136 NECFESHC
IPR001655 P47PHOX
IPR001683 Phox
IPR036871 PX_dom_sf
IPR034909 PX_p47phox
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR15706:SF6 PTHR15706:SF6, 1 hit
PfamiView protein in Pfam
PF16621 NECFESHC, 1 hit
PF08944 p47_phox_C, 1 hit
PF00787 PX, 1 hit
PF00018 SH3_1, 2 hits
PRINTSiPR00498 P47PHOX
PR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00312 PX, 1 hit
SM00326 SH3, 2 hits
SUPFAMiSSF50044 SSF50044, 2 hits
SSF64268 SSF64268, 1 hit
PROSITEiView protein in PROSITE
PS50195 PX, 1 hit
PS50002 SH3, 2 hits

Sequencei

Sequence statusi: Complete.

Q09014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDTFIRHIA LLGFEKRFIP SQHYVYMFLV KWQDLSEKVV YRKFTEIYEF
60 70 80 90 100
HKMLKEMFPI EAGEIHTENR VIPHLPAPRW FDGQRAAESR QGTLTEYFNG
110 120 130 140 150
LMGLPVKISR CPHLLDFFKV RPDDLKLPTD SQAKKPETYL VPKDGKNNVA
160 170 180 190 200
DITGPIILQT YRAIADYEKS SGTEMTVATG DVVDVVEKSE SGWWFCQMKT
210 220 230 240 250
KRGWVPASYL EPLDSPDEAE DPDPNYAGEP YVTIKAYAAV EEDEMSLSEG
260 270 280 290 300
EAIEVIHKLL DGWWVVRKGD ITGYFPSMYL QKAGEEITQA QRQIRGRGAP
310 320 330 340 350
PRRSTIRNAQ SIHQRSRKRL SQDTYRRNSV RFLQQRRRPG RPGPLSTDGT
360 370 380 390
KDNPSTPRVK PQPAVPPRPS SDLILHRCTE STKRKLTSAV
Length:390
Mass (Da):44,667
Last modified:July 27, 2011 - v3
Checksum:iC8EFAB953839CE9A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti161Y → H in AAA50469 (PubMed:8119734).Curated1
Sequence conflicti343 – 345GPL → RAA in AAA50469 (PubMed:8119734).Curated3
Sequence conflicti344P → Q in BAA25649 (PubMed:9490028).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11455 mRNA Translation: AAA50469.1
AB002663 mRNA Translation: BAA25649.1
AF267747 Genomic DNA Translation: AAF90134.1
AK149668 mRNA Translation: BAE29014.1
AK149732 mRNA Translation: BAE29053.1
AK152386 mRNA Translation: BAE31174.1
AK162308 mRNA Translation: BAE36845.1
AK170462 mRNA Translation: BAE41812.1
AK171559 mRNA Translation: BAE42526.1
CH466529 Genomic DNA Translation: EDL19452.1
CCDSiCCDS39298.1
RefSeqiNP_001272966.1, NM_001286037.1
NP_035006.3, NM_010876.4
UniGeneiMm.425296

Genome annotation databases

EnsembliENSMUST00000111275; ENSMUSP00000106906; ENSMUSG00000015950
ENSMUST00000146354; ENSMUSP00000138121; ENSMUSG00000015950
GeneIDi17969
KEGGimmu:17969
UCSCiuc008zvh.3 mouse

Similar proteinsi

Entry informationi

Entry nameiNCF1_MOUSE
AccessioniPrimary (citable) accession number: Q09014
Secondary accession number(s): O70144, Q3UE58, Q9JI34
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 27, 2011
Last modified: February 28, 2018
This is version 148 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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