Reviewed,
UniProtKB/Swiss-Prot Q08J23 (NSUN2_HUMAN)
Last modified
January 19, 2010.
Version 39.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: tRNA (cytosine-5-)-methyltransferase NSUN2 EC=2.1.1.29 Alternative name(s): NOL1/NOP2/Sun domain family member 2 tRNA (cytosine-5-)-methyltransferase tRNA methyltransferase 4 homolog Short name=hTrm4 Substrate of AIM1/Aurora kinase B | ||||
| Gene names |
| ||||
| Organism | Homo sapiens (Human) [Complete proteome] | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 767 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Not able to modify tRNAs at positions 48 or 49. May act downstream of Myc to regulate epidermal cell growth and proliferation. Ref.6 |
| Catalytic activity | S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing 5-methylcytosine. |
| Subunit structure | Interacts with NPM1 and NCL during interphase; interaction is disrupted following phosphorylation at Ser-139. Ref.1 |
| Subcellular location | Nucleus › nucleolus. Cytoplasm. Note: Concentrated in the nucleolus during interphase and distributed in the perichromosome and cytoplasm during mitosis. Ref.1 |
| Post-translational modification | Phosphorylated at Ser-139 by Aurora-B/STK12 during mitosis, leading to abolish methyltransferase activity and the interaction with NPM1. Ref.1 Ref.4 Ref.5 Ref.7 Ref.8 Ref.9 Ref.11 Ref.12 |
| Sequence similarities | Belongs to the methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | tRNA processing |
| Cellular component | Cytoplasm Nucleus |
| Coding sequence diversity | Polymorphism |
| Ligand | RNA-binding S-adenosyl-L-methionine tRNA-binding |
| Molecular function | Methyltransferase Transferase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | tRNA methylation Ref.6 Inferred from direct assay. Source: UniProtKB |
| Cellular component | cytoplasm Ref.6 Inferred from direct assay. Source: UniProtKB nucleolus Ref.6Inferred from direct assay. Source: UniProtKB |
| Molecular function | tRNA (cytosine-5-)-methyltransferase activity Ref.6 Inferred from direct assay. Source: UniProtKB tRNA bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 767 | 767 | tRNA (cytosine-5-)-methyltransferase NSUN2 | PRO_0000289223 | |||||
Regions | |||||||||
| Region | 184 – 190 | 7 | S-adenosyl-L-methionine binding By similarity | ||||||
Sites | |||||||||
| Active site | 321 | 1 | Nucleophile Potential | ||||||
| Binding site | 215 | 1 | S-adenosyl-L-methionine By similarity | ||||||
| Binding site | 242 | 1 | S-adenosyl-L-methionine By similarity | ||||||
| Binding site | 268 | 1 | S-adenosyl-L-methionine By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 139 | 1 | Phosphoserine; by STK12 Ref.1 | ||||||
| Modified residue | 456 | 1 | Phosphoserine Ref.4 Ref.9 Ref.11 | ||||||
| Modified residue | 461 | 1 | Phosphothreonine Ref.7 | ||||||
| Modified residue | 473 | 1 | Phosphoserine Ref.9 | ||||||
| Modified residue | 593 | 1 | Phosphoserine Ref.9 Ref.12 | ||||||
| Modified residue | 724 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 743 | 1 | Phosphoserine Ref.4 Ref.5 Ref.8 Ref.9 Ref.11 Ref.12 | ||||||
| Modified residue | 751 | 1 | Phosphoserine Ref.4 Ref.5 Ref.8 Ref.9 Ref.11 Ref.12 | ||||||
Natural variations | |||||||||
| Natural variant | 627 | 1 | V → I: dbSNP rs2303708. | VAR_032604 | |||||
Experimental info | |||||||||
| Mutagenesis | 139 | 1 | S → A: Induces a constitutive association with NPM1. Ref.1 | ||||||
| Mutagenesis | 139 | 1 | S → E: Mimicks constitutive phosphorylation and abolishes methyltransferase activity. Ref.1 | ||||||
| Sequence conflict | 316 | 1 | M → V in BAA91075. Ref.3 | ||||||
| Sequence conflict | 594 | 1 | G → D in BAB14762. Ref.3 | ||||||
| Sequence conflict | 605 | 1 | Q → R in BAF34150. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Aurora-B regulates RNA methyltransferase NSUN2." Sakita-Suto S., Kanda A., Suzuki F., Sato S., Takata T., Tatsuka M. Mol. Biol. Cell 18:1107-1117(2007) [PubMed: 17215513] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-139, INTERACTION WITH NPM1 AND NCL, MUTAGENESIS OF SER-139. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung and Placenta. |
| [3] | "Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 209-767. |
| [4] | "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-456; SER-743 AND SER-751, MASS SPECTROMETRY. Tissue: Epithelium. |
| [5] | "A probability-based approach for high-throughput protein phosphorylation analysis and site localization." Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P. Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-743 AND SER-751, MASS SPECTROMETRY. Tissue: Epithelium. |
| [6] | "Identification of human tRNA:m5C methyltransferase catalysing intron-dependent m5C formation in the first position of the anticodon of the pre-tRNA Leu (CAA)." Brzezicha B., Schmidt M., Makalowska I., Jarmolowski A., Pienkowska J., Szweykowska-Kulinska Z. Nucleic Acids Res. 34:6034-6043(2006) [PubMed: 17071714] [Abstract] Cited for: FUNCTION. |
| [7] | "Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry." Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A. Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007) [PubMed: 17287340] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-461, MASS SPECTROMETRY. |
| [8] | "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-743 AND SER-751, MASS SPECTROMETRY. |
| [9] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-456; SER-473; SER-593; SER-743 AND SER-751, MASS SPECTROMETRY. |
| [10] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| [11] | "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-456; SER-743 AND SER-751, MASS SPECTROMETRY. |
| [12] | "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed: 19690332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-593; SER-743 AND SER-751, MASS SPECTROMETRY. Tissue: T-cell. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB255451 mRNA. Translation: BAF34150.1. BC001041 mRNA. Translation: AAH01041.3. BC137083 mRNA. Translation: AAI37084.1. AK000310 mRNA. Translation: BAA91075.1. Different initiation. AK023994 mRNA. Translation: BAB14762.1. Different initiation. |
| IPI | IPI00306369. |
| RefSeq | NP_060225.4. |
| UniGene | Hs.481526 |
3D structure databases | |
| SMR | Q08J23. Positions 58-429. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q08J23. |
Proteomic databases | |
| PeptideAtlas | Q08J23. |
| PRIDE | Q08J23. |
Genome annotation databases | |
| Ensembl | ENST00000264670; ENSP00000264670; ENSG00000037474; Homo sapiens. [Genome view] |
| GeneID | 54888. |
| KEGG | hsa:54888. |
| NMPDR | fig|9606.3.peg.25032. |
| UCSC | uc003jdu.1. human. |
Organism-specific databases | |
| CTD | 54888. |
| GeneCards | GC05M006654. |
| HGNC | HGNC:25994. NSUN2. |
| MIM | 610916. gene. |
| PharmGKB | PA134953940. |
| GenAtlas | Search... |
Phylogenomic databases | |
| eggNOG | prNOG04966. |
| HOGENOM | HBG315458. |
| HOVERGEN | Q08J23. |
| InParanoid | Q08J23. |
| OMA | TYSQAKD. |
| OrthoDB | EOG93N9ZP. |
| PhylomeDB | Q08J23. |
Enzyme and pathway databases | |
| BRENDA | 2.1.1.29. 247. |
| Pathway_Interaction_DB | aurora_b_pathway. Aurora B signaling. |
Gene expression databases | |
| ArrayExpress | Q08J23. |
| Bgee | Q08J23. |
| CleanEx | HS_NSUN2. |
| Genevestigator | Q08J23. |
Family and domain databases | |
| InterPro | IPR001678. Fmu/NOL1/Nop2p. [Graphical view] |
| Pfam | PF01189. Nol1_Nop2_Fmu. 1 hit. [Graphical view] |
| PROSITE | PS01153. NOL1_NOP2_SUN. False negative. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 57879. |
| SOURCE | Search... |
Entry information
| Entry name | NSUN2_HUMAN | ||||||||
| Accession | Primary (citable) accession number: Q08J23 Secondary accession number(s): B2RNR4 Q9NXD9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
| Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. | ||||||||
Relevant documents
| Human chromosome 5 Human chromosome 5: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


