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Protein

Cas scaffolding protein family member 4

Gene

Cass4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Docking protein that plays a role in tyrosine kinase-based signaling related to cell adhesion and cell spreading. Regulates PTK2/FAK1 activity, focal adhesion integrity, and cell spreading (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Names & Taxonomyi

Protein namesi
Recommended name:
Cas scaffolding protein family member 4Imported
Gene namesi
Name:Cass4Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2444482. Cass4.

Subcellular locationi

  • Cytoplasmcytoskeleton By similarity
  • Cell junctionfocal adhesion By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 804804Cas scaffolding protein family member 4PRO_0000351205Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei200 – 2001PhosphoserineBy similarity
Modified residuei297 – 2971PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated on tyrosines by SRC.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ08EC4.
PaxDbiQ08EC4.
PRIDEiQ08EC4.

PTM databases

PhosphoSiteiQ08EC4.

Expressioni

Gene expression databases

BgeeiQ08EC4.

Interactioni

Subunit structurei

Interacts (via SH3 domain) with PTK2/FAK1 (via C-terminus).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ08EC4.
SMRiQ08EC4. Positions 14-71, 429-587, 700-803.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 7363SH3PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi379 – 42749Ser-richSequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the CAS family.Sequence analysis
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

eggNOGiENOG410IG8H. Eukaryota.
ENOG410XSP8. LUCA.
GeneTreeiENSGT00490000043324.
HOGENOMiHOG000111312.
HOVERGENiHBG107575.
InParanoidiQ08EC4.
OMAiCSDELAF.
OrthoDBiEOG7QRQTD.
PhylomeDBiQ08EC4.
TreeFamiTF328782.

Family and domain databases

InterProiIPR021901. CAS_DUF3513.
IPR014928. Serine_rich.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF12026. DUF3513. 1 hit.
PF08824. Serine_rich. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q08EC4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGTSIREGA PKTLLARALY DNHADCSDEL AFSRGDILTI VEQNVPESEG
60 70 80 90 100
WWRCLLHGRQ GLAPANRLQV LRETPADRPC PLLPRGPDTD LTSSGAPYQV
110 120 130 140 150
QDLISPPPQG PVYEPMRSWV EGPSPATAQV YELPESPSSA RIICEKTLSF
160 170 180 190 200
PKQALSVLPR PTRASLPTLP SQVYDVPVQR QGFSTLERLE KQQFYDIPTS
210 220 230 240 250
SQKALLHSST SQGRDVTLAP TMAFRQGGGY NPLSSPQKSE RIHDTPVLLE
260 270 280 290 300
KADVRNVSMT SFTKDSGSRA IPGSSAVHTG AVALSPQLGN TVQRKNSLPE
310 320 330 340 350
EPTYAFPTSR DPLPSDAGGS YKVPSRFLIP RVEQQNTMPN IYDTPKAMQG
360 370 380 390 400
VSHNAPKAMQ GVSLAGKELE RGREAPENSP WISGQTSFLS PDSDRLSVAS
410 420 430 440 450
SDSRASVVSS CSSISMDSSS GSSSEDSVKE LWMDVDFAKE TAVSLQHKVA
460 470 480 490 500
SSAAGLLLFV SRTWRFKDSL ETNIHRIRRA ADHVEESVRE FLDFAQGVGG
510 520 530 540 550
TACNLTDSYL QARIRDQLQT ISSSYQTLLD AKGSLDRCNW SLEVLVTDKV
560 570 580 590 600
QNSLDDLERF VATARIVPED VKRFTSIVIA NGKLLFKQNC EKGEMDLKCE
610 620 630 640 650
RCIRPPQRET ESYQESSPFD RQPTTEHSFE LARKNRVNVC WQQSPNLQEK
660 670 680 690 700
GKPTMEGKSN RNPDFHGMSP PPLTSPSPSG QNTERKIHLS KHSRLYFGAL
710 720 730 740 750
FKAISVFASS LSNGQPPEVF ITQSKLVITV GQKLVDTLCS ETQEKDERNE
760 770 780 790 800
ILCGSSHLCG LLKDLALATK SAVIQYPSPS ALSLLQSEVE RLEHHSRKFR

DTLE
Note: No experimental confirmation available.Curated
Length:804
Mass (Da):88,621
Last modified:October 31, 2006 - v1
Checksum:iAAC670C0ACA5FAD9
GO
Isoform 21 Publication (identifier: Q08EC4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     212-212: Q → QAS
     643-668: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:780
Mass (Da):85,869
Checksum:i5A7EA41AD88C4C89
GO
Isoform 31 Publication (identifier: Q08EC4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     643-685: QSPNLQEKGK...PSPSGQNTER → VSQGDKQYTG...HLHVHGEAIG
     686-804: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:685
Mass (Da):75,576
Checksum:iF2A8E415ADCF6F75
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei212 – 2121Q → QAS in isoform 2. 1 PublicationVSP_052948
Alternative sequencei643 – 68543QSPNL…QNTER → VSQGDKQYTGDFICYLNWSS LRQMLASCACRNVHLHVHGE AIG in isoform 3. 1 PublicationVSP_052949Add
BLAST
Alternative sequencei643 – 66826Missing in isoform 2. 1 PublicationVSP_052950Add
BLAST
Alternative sequencei686 – 804119Missing in isoform 3. 1 PublicationVSP_052951Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK170726 mRNA. Translation: BAE41983.1.
AL833787 Genomic DNA. Translation: CAM18024.1.
AL833787 Genomic DNA. Translation: CAM18025.1.
BC117858 mRNA. Translation: AAI17859.1.
BC117859 mRNA. Translation: AAI17860.1.
BC129975 mRNA. Translation: AAI29976.1.
BC129976 mRNA. Translation: AAI29977.1.
CCDSiCCDS38347.1. [Q08EC4-1]
RefSeqiNP_001074289.1. NM_001080820.2. [Q08EC4-1]
NP_001263351.1. NM_001276422.1. [Q08EC4-2]
NP_001263352.1. NM_001276423.1.
UniGeneiMm.210195.

Genome annotation databases

EnsembliENSMUST00000099061; ENSMUSP00000096660; ENSMUSG00000074570. [Q08EC4-3]
ENSMUST00000109136; ENSMUSP00000104764; ENSMUSG00000074570. [Q08EC4-1]
GeneIDi320664.
KEGGimmu:320664.
UCSCiuc008ocr.1. mouse. [Q08EC4-3]
uc008ocs.2. mouse. [Q08EC4-1]
uc008oct.2. mouse. [Q08EC4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK170726 mRNA. Translation: BAE41983.1.
AL833787 Genomic DNA. Translation: CAM18024.1.
AL833787 Genomic DNA. Translation: CAM18025.1.
BC117858 mRNA. Translation: AAI17859.1.
BC117859 mRNA. Translation: AAI17860.1.
BC129975 mRNA. Translation: AAI29976.1.
BC129976 mRNA. Translation: AAI29977.1.
CCDSiCCDS38347.1. [Q08EC4-1]
RefSeqiNP_001074289.1. NM_001080820.2. [Q08EC4-1]
NP_001263351.1. NM_001276422.1. [Q08EC4-2]
NP_001263352.1. NM_001276423.1.
UniGeneiMm.210195.

3D structure databases

ProteinModelPortaliQ08EC4.
SMRiQ08EC4. Positions 14-71, 429-587, 700-803.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ08EC4.

Proteomic databases

MaxQBiQ08EC4.
PaxDbiQ08EC4.
PRIDEiQ08EC4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000099061; ENSMUSP00000096660; ENSMUSG00000074570. [Q08EC4-3]
ENSMUST00000109136; ENSMUSP00000104764; ENSMUSG00000074570. [Q08EC4-1]
GeneIDi320664.
KEGGimmu:320664.
UCSCiuc008ocr.1. mouse. [Q08EC4-3]
uc008ocs.2. mouse. [Q08EC4-1]
uc008oct.2. mouse. [Q08EC4-2]

Organism-specific databases

CTDi57091.
MGIiMGI:2444482. Cass4.

Phylogenomic databases

eggNOGiENOG410IG8H. Eukaryota.
ENOG410XSP8. LUCA.
GeneTreeiENSGT00490000043324.
HOGENOMiHOG000111312.
HOVERGENiHBG107575.
InParanoidiQ08EC4.
OMAiCSDELAF.
OrthoDBiEOG7QRQTD.
PhylomeDBiQ08EC4.
TreeFamiTF328782.

Miscellaneous databases

NextBioi397171.
PROiQ08EC4.
SOURCEiSearch...

Gene expression databases

BgeeiQ08EC4.

Family and domain databases

InterProiIPR021901. CAS_DUF3513.
IPR014928. Serine_rich.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF12026. DUF3513. 1 hit.
PF08824. Serine_rich. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: NODImported.
    Tissue: Dendritic cellImported.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-297, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Lung and Spleen.

Entry informationi

Entry nameiCASS4_MOUSE
AccessioniPrimary (citable) accession number: Q08EC4
Secondary accession number(s): A1L397, Q08EC3, Q3TCH3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 23, 2008
Last sequence update: October 31, 2006
Last modified: November 11, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.