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Protein

CREB-regulated transcription coactivator 2

Gene

CRTC2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates gluconeogenesis as a component of the LKB1/AMPK/TORC2 signaling pathway. Regulates the expression of specific genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei629Required for ubiquitination and degradationBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
CREB-regulated transcription coactivator 2
Alternative name(s):
Transducer of regulated cAMP response element-binding protein 2
Short name:
TORC-2
Short name:
Transducer of CREB protein 2
Gene namesi
Name:CRTC2
Synonyms:TORC2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Translocated from the nucleus to the cytoplasm on interaction of the phosphorylated form with 14-3-3 protein. In response to cAMP levels and glucagon, relocated to the nucleus.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003185272 – 693CREB-regulated transcription coactivator 2Add BLAST692

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei51Asymmetric dimethylarginine; by PRMT6By similarity1
Modified residuei70PhosphoserineBy similarity1
Modified residuei86PhosphoserineBy similarity1
Modified residuei90PhosphoserineBy similarity1
Modified residuei99Asymmetric dimethylarginine; by PRMT6By similarity1
Modified residuei120Asymmetric dimethylarginine; by PRMT6By similarity1
Modified residuei123Asymmetric dimethylarginine; by PRMT6By similarity1
Modified residuei136PhosphoserineBy similarity1
Modified residuei161Asymmetric dimethylarginine; by PRMT6By similarity1
Modified residuei168Asymmetric dimethylarginine; by PRMT6By similarity1
Modified residuei171Phosphoserine; by AMPK, MARK2, SIK1 and SIK2By similarity1
Modified residuei274Phosphoserine; by MARK2By similarity1
Modified residuei306PhosphoserineBy similarity1
Modified residuei368PhosphoserineBy similarity1
Modified residuei393PhosphoserineBy similarity1
Modified residuei433PhosphoserineBy similarity1
Modified residuei456PhosphoserineBy similarity1
Modified residuei488PhosphotyrosineBy similarity1
Modified residuei489PhosphoserineBy similarity1
Modified residuei492PhosphoserineBy similarity1
Modified residuei501PhosphothreonineBy similarity1
Modified residuei613PhosphoserineBy similarity1
Modified residuei624PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation/dephosphorylation states of Ser-171 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation, is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs (SIK1 and SIK2) by LKB1. Both insulin and AMPK increase this phosphorylation of CRTC2 while glucagon suppresses it. Phosphorylation at Ser-274 by MARK2 is induced under low glucose conditions and dephosphorylated in response to glucose influx. Phosphorylation at Ser-274 promotes interaction with 14-3-3 proteins and translocation to the cytoplasm (By similarity).By similarity
Asymmetric dimethylation of arginine resisues by PRMT6 enhances the association of CRTC2 with CREB on the promoters of gluconeogenic genes.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ08E26.
PeptideAtlasiQ08E26.
PRIDEiQ08E26.

Expressioni

Gene expression databases

BgeeiENSBTAG00000017942.

Interactioni

Subunit structurei

Binds, as a tetramer, through its N-terminal region, with the bZIP domain of CREB1. 'Arg-314' in the bZIP domain of CREB1 is essential for this interaction. Interaction, via its C-terminal, with TAF4, enhances recruitment of TAF4 to CREB1 (By similarity). Interacts with PPP3CA/calcineurin alpha, SIK2 and 14-3-3 proteins, YWHAB and YWHAG. Interaction with RFWD2/COP1 mediates nuclear export and degradation of CRTC2 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000050880.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni209 – 215Required for interaction with RFWD2/COP1By similarity7

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi271 – 287Nuclear export signalBy similarityAdd BLAST17

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi236 – 240Poly-Ser5
Compositional biasi336 – 408Ser-richAdd BLAST73

Sequence similaritiesi

Belongs to the TORC family.Curated

Phylogenomic databases

eggNOGiENOG410IGNT. Eukaryota.
ENOG410XPDQ. LUCA.
GeneTreeiENSGT00390000010652.
HOGENOMiHOG000111980.
HOVERGENiHBG058314.
InParanoidiQ08E26.
KOiK16333.
OMAiHTLNHQN.
OrthoDBiEOG091G03YN.
TreeFamiTF321571.

Family and domain databases

InterProiIPR024786. TORC.
IPR024785. TORC_C.
IPR024784. TORC_M.
IPR024783. TORC_N.
[Graphical view]
PANTHERiPTHR13589. PTHR13589. 1 hit.
PfamiPF12886. TORC_C. 1 hit.
PF12885. TORC_M. 1 hit.
PF12884. TORC_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08E26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATSGANGPG SATASASNPR KFSEKIALQK QRQAEETAAF EEVMMDIGST
60 70 80 90 100
RLQAQKLRLA YTRSSHYGGS LPNVNQIGCG LAEFQSPLHS PLDSSRSTRH
110 120 130 140 150
HGLVERVQRD PRRMVSPLRR YARHIDSSPY SPAYLSPPPE SSWRRTMPWG
160 170 180 190 200
SFPAEKGQLF RLPSALNRTS SDSALHTSVM NPSPQDTYPS PAAPSVLPSR
210 220 230 240 250
RGGCLDGETD SKVPAIEENL LDDKHLLKPW DAKKLSSSSS RPRSCEVPGI
260 270 280 290 300
NIFPSPDQPA TVPVLPPAMN TGGSLPDLTN LHFPPPLPTP LDPEETAYPS
310 320 330 340 350
LSGGSSTSNL THTMTHLGIS GGLALGPGYD APGLHSPLSH PSFQSSLSNP
360 370 380 390 400
NLQASLSSPQ PQLQGSHSHP SLPASSLARH ALPTTSLGHP SLSAPALSSS
410 420 430 440 450
SSSSSASSPV LGAPAYPASA PGASPRHRRV PLSPLSLPAG PADARRSQQQ
460 470 480 490 500
LPKQFSPTMS PTLSSITQGV ALDTSKLPTD QRLPPYPYSP PSLVLPTQQP
510 520 530 540 550
TPKPLQQPGL PSQACSVQPS GGQPPGRQLQ YGTLYPPGPS GHGQQSYHRS
560 570 580 590 600
MSDFSLGNLE QFNMENPSTS LALDPPGFSE GPGFLGGEGP VSGPQDPHAL
610 620 630 640 650
NHQNVTHCSR HGSGPNVILT GDASPGFSKE IAAALAGVPG FEVSAAGLGL
660 670 680 690
GLGLEEELRM EPLGLEGLSM LSDPCALLPD PAVEDSFRSD RLQ
Length:693
Mass (Da):72,818
Last modified:October 31, 2006 - v1
Checksum:i497F16B29F62C135
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC123454 mRNA. Translation: AAI23455.1.
RefSeqiNP_001069718.1. NM_001076250.1.
UniGeneiBt.27312.

Genome annotation databases

EnsembliENSBTAT00000056382; ENSBTAP00000050880; ENSBTAG00000017942.
GeneIDi540959.
KEGGibta:540959.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC123454 mRNA. Translation: AAI23455.1.
RefSeqiNP_001069718.1. NM_001076250.1.
UniGeneiBt.27312.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000050880.

Proteomic databases

PaxDbiQ08E26.
PeptideAtlasiQ08E26.
PRIDEiQ08E26.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000056382; ENSBTAP00000050880; ENSBTAG00000017942.
GeneIDi540959.
KEGGibta:540959.

Organism-specific databases

CTDi200186.

Phylogenomic databases

eggNOGiENOG410IGNT. Eukaryota.
ENOG410XPDQ. LUCA.
GeneTreeiENSGT00390000010652.
HOGENOMiHOG000111980.
HOVERGENiHBG058314.
InParanoidiQ08E26.
KOiK16333.
OMAiHTLNHQN.
OrthoDBiEOG091G03YN.
TreeFamiTF321571.

Gene expression databases

BgeeiENSBTAG00000017942.

Family and domain databases

InterProiIPR024786. TORC.
IPR024785. TORC_C.
IPR024784. TORC_M.
IPR024783. TORC_N.
[Graphical view]
PANTHERiPTHR13589. PTHR13589. 1 hit.
PfamiPF12886. TORC_C. 1 hit.
PF12885. TORC_M. 1 hit.
PF12884. TORC_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCRTC2_BOVIN
AccessioniPrimary (citable) accession number: Q08E26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 31, 2006
Last modified: October 5, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.