Reviewed, UniProtKB/Swiss-Prot Q08E15 (SIA7F_BOVIN)

Names and origin

Protein names

Recommended name:
    Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6
    EC=2.4.99.-
Alternative name(s):
    GalNAc alpha-2,6-sialyltransferase VI
    ST6GalNAc VI
    Sialyltransferase 7F

Gene names

Name:	ST6GALNAC6
Synonyms:	SIAT7F

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Alpha-2,6-sialyltransferase involved in the synthesis of alpha-series gangliosides. Has activity toward GD1a, GT1b and GM1b. Has no activity toward glycoproteins. Responsible for the biosynthesis of DSGG (disialylgalactosylgloboside) from MSGG (monosialylgalactosylgloboside) in kidney By similarity.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 29 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to Golgi membrane Inferred from electronic annotation. Source: InterPro
Molecular function	sialyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6		PRO_0000314794
Regions
	Topological domain	1 – 42	42	Cytoplasmic Potential
	Transmembrane	43 – 63	21	Signal-anchor for type II membrane protein Potential
	Topological domain	64 – 332	269	Lumenal Potential
	Compositional bias	29 – 32	4	Poly-Arg
	Compositional bias	59 – 62	4	Poly-Ser
Amino acid modifications
	Glycosylation	97	1	N-linked (GlcNAc...) Potential
	Disulfide bond	107 ↔ 255		By similarity
Experimental info
	Sequence conflict	330	1	S → F in CAF06586. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q08E15-1 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: 990B326431CD62D3	FASTA	332	38,034
	10 20 30 40 50 60 MACPRPLSQC DHTPLPGPPA GHWPLPLSRR RREMKSNKEQ RSAVFVILFA LITILILYSS 70 80 90 100 110 120 SSANEVFHYG SLRGRTRRPV NLRKWSITDG YIPILGNKTL PSRCGQCVIV TSSSHLLGTK 130 140 150 160 170 180 LGPEIERAEC TIRMNDAPTT GYSADVGNKT TFRVVAHSSV FHVLRRPQEF VNRTPETVFI 190 200 210 220 230 240 FWGPPNKMQK PQGSLVRVIQ RAGLVFPNME AYAISLSRMR QFDDLFRSET GKDREKSHSW 250 260 270 280 290 300 LSTGWFTMVI AVELCDHVHV YGMVPPDYCS LRPHLQRMPY HYYEPKGPDE CVTYIQNENS 310 320 330 RKGNHHRFIT EKRVFSSWAQ LYGITFSHPS WT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach." Harduin-Lepers A., Mollicone R., Delannoy P., Oriol R. Glycobiology 15:805-817(2005) [PubMed: 15843597] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Basal ganglia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ620949 mRNA. Translation: CAF06586.1. BC123467 mRNA. Translation: AAI23468.1.
IPI	IPI00716363.
RefSeq	NP_001001151.2.
UniGene	Bt.64800
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT29. Glycosyltransferase Family 29.
Genome annotation databases
GeneID	407222.
KEGG	bta:407222.
Organism-specific databases
CTD	407222.
Phylogenomic databases
HOVERGEN	Q08E15.
Family and domain databases
InterPro	IPR001675. Glyco_trans_29. [Graphical view]
Pfam	PF00777. Glyco_transf_29. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SIA7F_BOVIN

Accession

Primary (citable) accession number: Q08E15
Secondary accession number(s): Q704S2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 31, 2006
Last modified:	September 1, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)