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Protein

Serine/threonine-protein kinase PRP4 homolog

Gene

PRPF4B

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Has a role in pre-mRNA splicing. Phosphorylates SF2/ASF (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei718ATPPROSITE-ProRule annotation1
Active sitei816Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi694 – 702ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PRP4 homolog (EC:2.7.11.1)
Alternative name(s):
PRP4 pre-mRNA-processing factor 4 homolog
Gene namesi
Name:PRPF4B
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 23

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003261382 – 1008Serine/threonine-protein kinase PRP4 homologAdd BLAST1007

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei8PhosphoserineBy similarity1
Modified residuei20PhosphoserineBy similarity1
Modified residuei23PhosphoserineBy similarity1
Modified residuei32PhosphoserineBy similarity1
Modified residuei87PhosphoserineBy similarity1
Modified residuei93PhosphoserineBy similarity1
Modified residuei99N6-acetyllysineBy similarity1
Cross-linki117Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki117Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei131PhosphoserineBy similarity1
Modified residuei140PhosphotyrosineBy similarity1
Modified residuei142PhosphoserineBy similarity1
Modified residuei144PhosphoserineBy similarity1
Modified residuei166PhosphoserineBy similarity1
Cross-linki170Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei239PhosphoserineBy similarity1
Modified residuei241PhosphoserineBy similarity1
Modified residuei257PhosphoserineBy similarity1
Modified residuei277PhosphoserineBy similarity1
Modified residuei283PhosphoserineBy similarity1
Modified residuei292PhosphoserineBy similarity1
Modified residuei294PhosphoserineBy similarity1
Modified residuei328PhosphoserineBy similarity1
Modified residuei354PhosphoserineBy similarity1
Modified residuei356PhosphoserineBy similarity1
Modified residuei366PhosphoserineBy similarity1
Modified residuei368PhosphoserineBy similarity1
Modified residuei385PhosphothreonineBy similarity1
Modified residuei387PhosphoserineBy similarity1
Modified residuei427PhosphoserineBy similarity1
Modified residuei431PhosphoserineBy similarity1
Modified residuei437PhosphoserineBy similarity1
Modified residuei519PhosphoserineBy similarity1
Modified residuei520PhosphoserineBy similarity1
Modified residuei521PhosphoserineBy similarity1
Modified residuei566PhosphoserineBy similarity1
Modified residuei570PhosphoserineBy similarity1
Modified residuei577PhosphoserineBy similarity1
Modified residuei579PhosphoserineBy similarity1
Modified residuei581PhosphoserineBy similarity1
Modified residuei718N6-acetyllysineBy similarity1
Modified residuei850PhosphotyrosineBy similarity1
Modified residuei853PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by Clk1.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ08DZ2.
PRIDEiQ08DZ2.

Expressioni

Gene expression databases

BgeeiENSBTAG00000002086.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Interacts with Clk1 C-terminus (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000002695.

Structurei

3D structure databases

ProteinModelPortaliQ08DZ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini688 – 1004Protein kinasePROSITE-ProRule annotationAdd BLAST317

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi39 – 497Arg/Lys-rich (basic)Add BLAST459
Compositional biasi40 – 78His-richAdd BLAST39

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0670. Eukaryota.
ENOG410XPAX. LUCA.
GeneTreeiENSGT00760000119032.
HOGENOMiHOG000043099.
HOVERGENiHBG055182.
InParanoidiQ08DZ2.
KOiK08827.
OMAiSGERDRY.
OrthoDBiEOG091G095F.
TreeFamiTF315246.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08DZ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAEAPSLR EQPEMEDANS EKSVNEENGE VSEDQSQNKH SRHKKKKHKH
60 70 80 90 100
RSKHKKHKHS SEEDKDRKHK HKHKHKKHKR KEVADASDKE GMSPAKRTKL
110 120 130 140 150
DDLALLEDLE KQRALIKAEL DNELMEGKVQ SGMGLILQGY ESGSEEEGEI
160 170 180 190 200
HEKARNGNRS STRSSSTKGK LELVDNKNST KKRSKSRSKE RTRHRSDKKK
210 220 230 240 250
SKGGVEIVKE KATRSKSKER KKSKSPSKRS KSQDEARKSK SPTLRRRSQE
260 270 280 290 300
KVGKARSPVD DKAKVEDKSK AKDRKKSPVI NESRSRDRGK KSRSPVDLRG
310 320 330 340 350
KSKDRRSRSK ERKSKRPEAD KEKKPVKSPS KDASSGKENR SPSRRPGRSP
360 370 380 390 400
KRRSLSPKQR DKSRRSRSPL VNDRRSKQSK SPSRTLSPGR RAKSRSLERK
410 420 430 440 450
RREPERRRLS SPRTRPRDDI LSRRERSKDA SPISRWSPAR RRASRSPVRR
460 470 480 490 500
RSRSPLRRSR SPRRRSRSPR RRDRGRRSRS RLRRRSRSRG GRRRRSRSKV
510 520 530 540 550
KEDKFKGSLS EGMKVEQESS SDDNLEDFDV EEEDEEALIE QRRIQRQAIV
560 570 580 590 600
QKYKYLADDS NLSVPSEPSS PQSSTRSRSP SPDDILERVA ADVKEYEREN
610 620 630 640 650
VDTFEASVKA KHNLMAVEQN NGSSQKKLLA PDMFTESDDM FAAYFDSARL
660 670 680 690 700
RAAGIGKDFK ENPNLRDNWT DAEGYYRVNI GEVLDKRYNV YGYTGQGVFS
710 720 730 740 750
NVVRARDNAR ANQEVAVKII RNNELMQKTG LKELEFLKKL NDADPDDKFH
760 770 780 790 800
CLRLFRHFYH KQHLCLVFEP LSMNLREVLK KYGKDVGLHI KAVRSYSQQL
810 820 830 840 850
FLALKLLKRC NILHADIKPD NILVNESKTI LKLCDFGSAS HVADNDITPY
860 870 880 890 900
LVSRFYRAPE IIIGKSYDYG IDMWSVGCTL YELYTGKILF PGKTNNHMLK
910 920 930 940 950
LAMDLKGKMP NKMIRKGVFK DQHFDQNLNF MYIEVDKVTE REKVTVMSTI
960 970 980 990 1000
NPTKDLLADL IGCQRLPEDQ RKKVHQLKDL LDQILMLDPA KRISINQALQ

HAFIQEKI
Length:1,008
Mass (Da):116,646
Last modified:October 31, 2006 - v1
Checksum:i81F59FAAE8729BF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC123499 mRNA. Translation: AAI23500.1.
RefSeqiNP_001069025.1. NM_001075557.1.
UniGeneiBt.9710.

Genome annotation databases

EnsembliENSBTAT00000002695; ENSBTAP00000002695; ENSBTAG00000002086.
GeneIDi512236.
KEGGibta:512236.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC123499 mRNA. Translation: AAI23500.1.
RefSeqiNP_001069025.1. NM_001075557.1.
UniGeneiBt.9710.

3D structure databases

ProteinModelPortaliQ08DZ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000002695.

Proteomic databases

PaxDbiQ08DZ2.
PRIDEiQ08DZ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000002695; ENSBTAP00000002695; ENSBTAG00000002086.
GeneIDi512236.
KEGGibta:512236.

Organism-specific databases

CTDi8899.

Phylogenomic databases

eggNOGiKOG0670. Eukaryota.
ENOG410XPAX. LUCA.
GeneTreeiENSGT00760000119032.
HOGENOMiHOG000043099.
HOVERGENiHBG055182.
InParanoidiQ08DZ2.
KOiK08827.
OMAiSGERDRY.
OrthoDBiEOG091G095F.
TreeFamiTF315246.

Gene expression databases

BgeeiENSBTAG00000002086.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRP4B_BOVIN
AccessioniPrimary (citable) accession number: Q08DZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: October 31, 2006
Last modified: October 5, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.