Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein kinase PRP4 homolog

Gene

PRPF4B

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Has a role in pre-mRNA splicing. Phosphorylates SF2/ASF (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei718 – 7181ATPPROSITE-ProRule annotation
Active sitei816 – 8161Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi694 – 7029ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PRP4 homolog (EC:2.7.11.1)
Alternative name(s):
PRP4 pre-mRNA-processing factor 4 homolog
Gene namesi
Name:PRPF4B
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 23

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 10081007Serine/threonine-protein kinase PRP4 homologPRO_0000326138Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei8 – 81PhosphoserineBy similarity
Modified residuei20 – 201PhosphoserineBy similarity
Modified residuei23 – 231PhosphoserineBy similarity
Modified residuei32 – 321PhosphoserineBy similarity
Modified residuei87 – 871PhosphoserineBy similarity
Modified residuei93 – 931PhosphoserineBy similarity
Modified residuei99 – 991N6-acetyllysineBy similarity
Cross-linki117 – 117Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei131 – 1311PhosphoserineBy similarity
Modified residuei140 – 1401PhosphotyrosineBy similarity
Modified residuei142 – 1421PhosphoserineBy similarity
Modified residuei144 – 1441PhosphoserineBy similarity
Cross-linki170 – 170Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei239 – 2391PhosphoserineBy similarity
Modified residuei241 – 2411PhosphoserineBy similarity
Modified residuei257 – 2571PhosphoserineBy similarity
Modified residuei277 – 2771PhosphoserineBy similarity
Modified residuei283 – 2831PhosphoserineBy similarity
Modified residuei294 – 2941PhosphoserineBy similarity
Modified residuei328 – 3281PhosphoserineBy similarity
Modified residuei354 – 3541PhosphoserineBy similarity
Modified residuei356 – 3561PhosphoserineBy similarity
Modified residuei366 – 3661PhosphoserineBy similarity
Modified residuei368 – 3681PhosphoserineBy similarity
Modified residuei385 – 3851PhosphothreonineBy similarity
Modified residuei387 – 3871PhosphoserineBy similarity
Modified residuei427 – 4271PhosphoserineBy similarity
Modified residuei431 – 4311PhosphoserineBy similarity
Modified residuei437 – 4371PhosphoserineBy similarity
Modified residuei519 – 5191PhosphoserineBy similarity
Modified residuei520 – 5201PhosphoserineBy similarity
Modified residuei521 – 5211PhosphoserineBy similarity
Modified residuei566 – 5661PhosphoserineBy similarity
Modified residuei570 – 5701PhosphoserineBy similarity
Modified residuei577 – 5771PhosphoserineBy similarity
Modified residuei579 – 5791PhosphoserineBy similarity
Modified residuei581 – 5811PhosphoserineBy similarity
Modified residuei718 – 7181N6-acetyllysineBy similarity
Modified residuei850 – 8501PhosphotyrosineBy similarity
Modified residuei853 – 8531PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by Clk1.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ08DZ2.
PRIDEiQ08DZ2.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Interacts with Clk1 C-terminus (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000002695.

Structurei

3D structure databases

ProteinModelPortaliQ08DZ2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini688 – 1004317Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi39 – 497459Arg/Lys-rich (basic)Add
BLAST
Compositional biasi40 – 7839His-richAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0670. Eukaryota.
ENOG410XPAX. LUCA.
GeneTreeiENSGT00580000081366.
HOGENOMiHOG000043099.
HOVERGENiHBG055182.
InParanoidiQ08DZ2.
KOiK08827.
OMAiSGERDRY.
OrthoDBiEOG76HQ1K.
TreeFamiTF315246.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08DZ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAEAPSLR EQPEMEDANS EKSVNEENGE VSEDQSQNKH SRHKKKKHKH
60 70 80 90 100
RSKHKKHKHS SEEDKDRKHK HKHKHKKHKR KEVADASDKE GMSPAKRTKL
110 120 130 140 150
DDLALLEDLE KQRALIKAEL DNELMEGKVQ SGMGLILQGY ESGSEEEGEI
160 170 180 190 200
HEKARNGNRS STRSSSTKGK LELVDNKNST KKRSKSRSKE RTRHRSDKKK
210 220 230 240 250
SKGGVEIVKE KATRSKSKER KKSKSPSKRS KSQDEARKSK SPTLRRRSQE
260 270 280 290 300
KVGKARSPVD DKAKVEDKSK AKDRKKSPVI NESRSRDRGK KSRSPVDLRG
310 320 330 340 350
KSKDRRSRSK ERKSKRPEAD KEKKPVKSPS KDASSGKENR SPSRRPGRSP
360 370 380 390 400
KRRSLSPKQR DKSRRSRSPL VNDRRSKQSK SPSRTLSPGR RAKSRSLERK
410 420 430 440 450
RREPERRRLS SPRTRPRDDI LSRRERSKDA SPISRWSPAR RRASRSPVRR
460 470 480 490 500
RSRSPLRRSR SPRRRSRSPR RRDRGRRSRS RLRRRSRSRG GRRRRSRSKV
510 520 530 540 550
KEDKFKGSLS EGMKVEQESS SDDNLEDFDV EEEDEEALIE QRRIQRQAIV
560 570 580 590 600
QKYKYLADDS NLSVPSEPSS PQSSTRSRSP SPDDILERVA ADVKEYEREN
610 620 630 640 650
VDTFEASVKA KHNLMAVEQN NGSSQKKLLA PDMFTESDDM FAAYFDSARL
660 670 680 690 700
RAAGIGKDFK ENPNLRDNWT DAEGYYRVNI GEVLDKRYNV YGYTGQGVFS
710 720 730 740 750
NVVRARDNAR ANQEVAVKII RNNELMQKTG LKELEFLKKL NDADPDDKFH
760 770 780 790 800
CLRLFRHFYH KQHLCLVFEP LSMNLREVLK KYGKDVGLHI KAVRSYSQQL
810 820 830 840 850
FLALKLLKRC NILHADIKPD NILVNESKTI LKLCDFGSAS HVADNDITPY
860 870 880 890 900
LVSRFYRAPE IIIGKSYDYG IDMWSVGCTL YELYTGKILF PGKTNNHMLK
910 920 930 940 950
LAMDLKGKMP NKMIRKGVFK DQHFDQNLNF MYIEVDKVTE REKVTVMSTI
960 970 980 990 1000
NPTKDLLADL IGCQRLPEDQ RKKVHQLKDL LDQILMLDPA KRISINQALQ

HAFIQEKI
Length:1,008
Mass (Da):116,646
Last modified:October 31, 2006 - v1
Checksum:i81F59FAAE8729BF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC123499 mRNA. Translation: AAI23500.1.
RefSeqiNP_001069025.1. NM_001075557.1.
UniGeneiBt.9710.

Genome annotation databases

EnsembliENSBTAT00000002695; ENSBTAP00000002695; ENSBTAG00000002086.
GeneIDi512236.
KEGGibta:512236.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC123499 mRNA. Translation: AAI23500.1.
RefSeqiNP_001069025.1. NM_001075557.1.
UniGeneiBt.9710.

3D structure databases

ProteinModelPortaliQ08DZ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000002695.

Proteomic databases

PaxDbiQ08DZ2.
PRIDEiQ08DZ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000002695; ENSBTAP00000002695; ENSBTAG00000002086.
GeneIDi512236.
KEGGibta:512236.

Organism-specific databases

CTDi8899.

Phylogenomic databases

eggNOGiKOG0670. Eukaryota.
ENOG410XPAX. LUCA.
GeneTreeiENSGT00580000081366.
HOGENOMiHOG000043099.
HOVERGENiHBG055182.
InParanoidiQ08DZ2.
KOiK08827.
OMAiSGERDRY.
OrthoDBiEOG76HQ1K.
TreeFamiTF315246.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Thalamus.

Entry informationi

Entry nameiPRP4B_BOVIN
AccessioniPrimary (citable) accession number: Q08DZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: October 31, 2006
Last modified: June 8, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.