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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2

Gene

GFPT2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins (By similarity).By similarity

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.

Pathway: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 (GFPT2)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_309938. Synthesis of UDP-N-acetyl-glucosamine.
SABIO-RKQ08DQ2.
UniPathwayiUPA00113; UER00528.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 (EC:2.6.1.16)
Alternative name(s):
D-fructose-6-phosphate amidotransferase 2
Glutamine:fructose-6-phosphate amidotransferase 2
Short name:
GFAT 2
Short name:
GFAT2
Hexosephosphate aminotransferase 2
Gene namesi
Name:GFPT2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 7

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 682681Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2PRO_0000287357Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei244 – 2441PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ08DQ2.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000002867.

Structurei

3D structure databases

ProteinModelPortaliQ08DQ2.
SMRiQ08DQ2. Positions 322-670.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 288287Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini360 – 499140SIS 1PROSITE-ProRule annotationAdd
BLAST
Domaini531 – 672142SIS 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation
Contains 2 SIS domains.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiCOG0449.
GeneTreeiENSGT00390000010049.
HOGENOMiHOG000258898.
HOVERGENiHBG051724.
InParanoidiQ08DQ2.
KOiK00820.
OMAiDCLEVFK.
OrthoDBiEOG73NG2S.
TreeFamiTF300864.

Family and domain databases

Gene3Di3.60.20.10. 2 hits.
InterProiIPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PfamiPF00310. GATase_2. 2 hits.
PF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q08DQ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIFAYMNY RVPRTRKEIF ETLIKGLQRL EYRGYDSAGV AIDGNNNEVK
60 70 80 90 100
ERHIQLVKKR GNVKALDEEL YKQDSMDLKV EFETHFGIAH TRWATHGVPS
110 120 130 140 150
AVNSHPQRSD KGNEFVVIHN GIITNYKDLR KFLESKGYEF ESETDTETIA
160 170 180 190 200
KLIKYVFDNR ETEDITFSTL VERVIQQLEG AFALVFKSIH YPGEAVATRR
210 220 230 240 250
GSPLLIGVRS KYKLSTEQIP VLYRTRNIEN VKNICKTRMK RLDSSTCLHA
260 270 280 290 300
VGNKAVEFFF ASDASAIIEH TNRVIFLEDD DIAAVADGKL SIHRVKRLAS
310 320 330 340 350
DDPSRAIQTL QMELQQIMKG NFSAFMQKEI FEQPESVFNT MRGRVNFETN
360 370 380 390 400
TVLLGGLKDH LKEIRRCRRL IVIGCGTSYH AAVATRQVLE ELTELPVMVE
410 420 430 440 450
LASDFLDRNT PVFRDDVCFF ISQSGETADT LLALRYCKDR RALTVGVTNT
460 470 480 490 500
VGSSISRETD CGVHINAGPE IGVASTKAYT SQFISLVMFG LMMSEDRISL
510 520 530 540 550
QNRRREIIHG LKSLPELIKE VLSLDEKIHD LALELYTQRS LLVMGRGYNY
560 570 580 590 600
ATCLEGALKI KEITYMHSEG ILAGELKHGP LALIDKQMPV IMVIMKDPCF
610 620 630 640 650
AKCQNALQQV TARQGRPIIL CSKDDTESSK FAYKTIELPH TVDCLQGILS
660 670 680
VIPLQLLSFH LAVLRGYDVD FPRNLAKSVT VE
Length:682
Mass (Da):77,081
Last modified:October 31, 2006 - v1
Checksum:i773A71B230746130
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC123624 mRNA. Translation: AAI23625.1.
RefSeqiNP_001070351.1. NM_001076883.1.
UniGeneiBt.8781.

Genome annotation databases

EnsembliENSBTAT00000002867; ENSBTAP00000002867; ENSBTAG00000002215.
GeneIDi530101.
KEGGibta:530101.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC123624 mRNA. Translation: AAI23625.1.
RefSeqiNP_001070351.1. NM_001076883.1.
UniGeneiBt.8781.

3D structure databases

ProteinModelPortaliQ08DQ2.
SMRiQ08DQ2. Positions 322-670.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000002867.

Proteomic databases

PRIDEiQ08DQ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000002867; ENSBTAP00000002867; ENSBTAG00000002215.
GeneIDi530101.
KEGGibta:530101.

Organism-specific databases

CTDi9945.

Phylogenomic databases

eggNOGiCOG0449.
GeneTreeiENSGT00390000010049.
HOGENOMiHOG000258898.
HOVERGENiHBG051724.
InParanoidiQ08DQ2.
KOiK00820.
OMAiDCLEVFK.
OrthoDBiEOG73NG2S.
TreeFamiTF300864.

Enzyme and pathway databases

UniPathwayiUPA00113; UER00528.
ReactomeiREACT_309938. Synthesis of UDP-N-acetyl-glucosamine.
SABIO-RKQ08DQ2.

Miscellaneous databases

NextBioi20875180.

Family and domain databases

Gene3Di3.60.20.10. 2 hits.
InterProiIPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PfamiPF00310. GATase_2. 2 hits.
PF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Ascending colon.

Entry informationi

Entry nameiGFPT2_BOVIN
AccessioniPrimary (citable) accession number: Q08DQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: October 31, 2006
Last modified: May 27, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.