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Protein

Heat shock factor protein 1

Gene

HSF1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi15 – 120By similarityAdd BLAST106

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-BTA-3371453. Regulation of HSF1-mediated heat shock response.
R-BTA-3371511. HSF1 activation.
R-BTA-3371568. Attenuation phase.
R-BTA-3371571. HSF1-dependent transactivation.

Names & Taxonomyi

Protein namesi
Recommended name:
Heat shock factor protein 1
Short name:
HSF 1
Alternative name(s):
Heat shock transcription factor 1
Short name:
HSTF 1
Gene namesi
Name:HSF1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 14

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002602631 – 525Heat shock factor protein 1Add BLAST525

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei121Phosphoserine; by MAPKAPK2By similarity1
Modified residuei142Phosphothreonine; by CK2By similarity1
Modified residuei230PhosphoserineBy similarity1
Modified residuei292PhosphoserineBy similarity1
Cross-linki298Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei303PhosphoserineBy similarity1
Modified residuei307PhosphoserineBy similarity1
Modified residuei314PhosphoserineBy similarity1
Modified residuei324PhosphothreonineBy similarity1
Modified residuei327PhosphoserineBy similarity1
Modified residuei346PhosphoserineBy similarity1
Modified residuei359PhosphoserineBy similarity1
Modified residuei415PhosphoserineBy similarity1
Modified residuei440PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat (By similarity).By similarity
Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ08DJ8.
PRIDEiQ08DJ8.

Expressioni

Gene expression databases

BgeeiENSBTAG00000020751.

Interactioni

Subunit structurei

Monomer (By similarity). Under normal conditions, interacts with HSP90AA1 in the HSP90 multichaperone complex; the interaction prevents trimerization and activation of HSF1. On activation by heat-stress or by other factors such as metal ions, HSF1 is released from the complex, homotrimerizes, is hyperphosphorylated and translocated to the nucleus where, subsequently, it can activate transcription. Binds the complex through the regulatory domain. Interacts with SYMPK and CSTF2 in heat-stressed cells. Interacts with FKBP4 in the HSP90 multichaperone complex; the interaction is independent of the phosphorylation state of HSF1. Interacts with MAPKAPK2. Interacts with EEF1D at heat shock promoter elements (HSE) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027654.

Structurei

3D structure databases

ProteinModelPortaliQ08DJ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni130 – 203Hydrophobic repeat HR-A/BAdd BLAST74
Regioni221 – 310Regulatory domainAdd BLAST90
Regioni367 – 525Transactivation domainAdd BLAST159
Regioni380 – 405Hydrophobic repeat HR-CAdd BLAST26

Sequence similaritiesi

Belongs to the HSF family.Curated

Phylogenomic databases

eggNOGiKOG0627. Eukaryota.
COG5169. LUCA.
GeneTreeiENSGT00390000001182.
HOGENOMiHOG000253917.
HOVERGENiHBG005999.
InParanoidiQ08DJ8.
KOiK09414.
OMAiQFSLEHV.
OrthoDBiEOG091G087O.
TreeFamiTF330401.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR027072. HSF1.
IPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR010542. Vert_HSTF_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 2 hits.
PTHR10015:SF182. PTHR10015:SF182. 2 hits.
PfamiPF00447. HSF_DNA-bind. 1 hit.
PF06546. Vert_HS_TF. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08DJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLPVGPGAA GPSNVPAFLT KLWTLVSDPD TDALICWSPS GNSFHVLDQG
60 70 80 90 100
QFAKEVLPKY FKHSNMASFV RQLNMYGFRK VVHIEQGGLV KPERDDTEFQ
110 120 130 140 150
HPCFLRGQEQ LLENIKRKVT SVSTLRSEDI KIRQDSVTKL LTDVQLMKGK
160 170 180 190 200
QESMDSKLLA MKHENEALWR EVASLRQKHA QQQKVVNKLI QFLISLVQSN
210 220 230 240 250
RILGVKRKIP LMLNDGGPAH PMPKYGRQYS LEHIHGPGPY PAPSPAYSGS
260 270 280 290 300
SLYSPDAVTS SGPIISDITE LAPGSPVASS GGSVDERPLS SSPLVRVKEE
310 320 330 340 350
PPSPPQSPRA EGASPGRPSS MVETPLSPTT LIDSILRESE PTPVASTTPL
360 370 380 390 400
VDTGGRPPSP LPASAPEKCL SVACLDKTEL SDHLDAMDSN LDNLQTMLTS
410 420 430 440 450
HGFSVDTSTL LDLFSPSVTV PDMSLPDLDS SLASIQELLS PQEPPRPLEA
460 470 480 490 500
EKSSPDSGKQ LVHYTAQPLL LLDPGSVDVG SSDLPVLFEL GEGSYFSEGD
510 520
DYSDDPTISL LTGSEPPKAK DPTVS
Length:525
Mass (Da):56,702
Last modified:October 31, 2006 - v1
Checksum:i985CF9E968B0040B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC123711 mRNA. Translation: AAI23712.1.
RefSeqiNP_001070277.1. NM_001076809.1.
UniGeneiBt.61991.

Genome annotation databases

EnsembliENSBTAT00000027654; ENSBTAP00000027654; ENSBTAG00000020751.
GeneIDi506235.
KEGGibta:506235.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC123711 mRNA. Translation: AAI23712.1.
RefSeqiNP_001070277.1. NM_001076809.1.
UniGeneiBt.61991.

3D structure databases

ProteinModelPortaliQ08DJ8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000027654.

Proteomic databases

PaxDbiQ08DJ8.
PRIDEiQ08DJ8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000027654; ENSBTAP00000027654; ENSBTAG00000020751.
GeneIDi506235.
KEGGibta:506235.

Organism-specific databases

CTDi3297.

Phylogenomic databases

eggNOGiKOG0627. Eukaryota.
COG5169. LUCA.
GeneTreeiENSGT00390000001182.
HOGENOMiHOG000253917.
HOVERGENiHBG005999.
InParanoidiQ08DJ8.
KOiK09414.
OMAiQFSLEHV.
OrthoDBiEOG091G087O.
TreeFamiTF330401.

Enzyme and pathway databases

ReactomeiR-BTA-3371453. Regulation of HSF1-mediated heat shock response.
R-BTA-3371511. HSF1 activation.
R-BTA-3371568. Attenuation phase.
R-BTA-3371571. HSF1-dependent transactivation.

Gene expression databases

BgeeiENSBTAG00000020751.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR027072. HSF1.
IPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR010542. Vert_HSTF_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 2 hits.
PTHR10015:SF182. PTHR10015:SF182. 2 hits.
PfamiPF00447. HSF_DNA-bind. 1 hit.
PF06546. Vert_HS_TF. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHSF1_BOVIN
AccessioniPrimary (citable) accession number: Q08DJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 31, 2006
Last modified: November 30, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.