Q08DA3 (UBP16_BOVIN) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 29, 2013.
Version 50.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Ubiquitin carboxyl-terminal hydrolase 16 EC=3.4.19.12 Alternative name(s): Deubiquitinating enzyme 16 Ubiquitin thioesterase 16 Ubiquitin-specific-processing protease 16 | ||
| Gene names |
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| Organism | Bos taurus (Bovine) [Reference proteome] | ||
| Taxonomic identifier | 9913 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Protein attributes
| Sequence length | 826 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Specifically deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-10' of histone H3, and is required for chromosome segregation when cells enter into mitosis. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B By similarity. |
| Catalytic activity | Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). |
| Subunit structure | Homotetramer By similarity. |
| Subcellular location | Nucleus By similarity. |
| Domain | The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin By similarity. |
| Post-translational modification | Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. The phosphorylated form of the protein is also enzymatically active By similarity. |
| Sequence similarities | Belongs to the peptidase C19 family. USP16 subfamily. Contains 1 UBP-type zinc finger. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 826 | 826 | Ubiquitin carboxyl-terminal hydrolase 16 | PRO_0000367501 | |||||
Regions | |||||||||
| Zinc finger | 60 – 125 | 66 | UBP-type | ||||||
Sites | |||||||||
| Active site | 204 | 1 | Nucleophile By similarity | ||||||
| Active site | 760 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 24 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 26 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 48 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 51 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 74 | 1 | Zinc 3 By similarity | ||||||
| Metal binding | 77 | 1 | Zinc 3 By similarity | ||||||
| Metal binding | 82 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 88 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 90 | 1 | Zinc 2 By similarity | ||||||
| Metal binding | 94 | 1 | Zinc 3 By similarity | ||||||
| Metal binding | 103 | 1 | Zinc 3 By similarity | ||||||
| Metal binding | 116 | 1 | Zinc 1 By similarity | ||||||
| Metal binding | 119 | 1 | Zinc 1 By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 414 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 551 | 1 | Phosphoserine By similarity | ||||||
Sequences
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References
| [1] | NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal muscle. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BC123861 mRNA. Translation: AAI23862.1. |
| IPI | IPI00706867. |
| RefSeq | NP_001070335.1. NM_001076867.1. |
| UniGene | Bt.51616. |
3D structure databases | |
| ProteinModelPortal | Q08DA3. |
| SMR | Q08DA3. Positions 22-142. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | 9913.ENSBTAP00000026801. |
Protein family/group databases | |
| MEROPS | C19.021. |
Proteomic databases | |
| PRIDE | Q08DA3. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 519992. |
| KEGG | bta:519992. |
Organism-specific databases | |
| CTD | 10600. |
Phylogenomic databases | |
| eggNOG | COG5207. |
| HOGENOM | HOG000154755. |
| HOVERGEN | HBG062704. |
| InParanoid | Q08DA3. |
| KO | K11844. |
| OrthoDB | EOG495ZRD. |
Enzyme and pathway databases | |
| BioCyc | CATTLE:519992-MONOMER. |
Family and domain databases | |
| Gene3D | 3.30.40.10. 1 hit. |
| InterPro | IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR013083. Znf_RING/FYVE/PHD. IPR001607. Znf_UBP. [Graphical view] |
| Pfam | PF00443. UCH. 1 hit. PF02148. zf-UBP. 1 hit. [Graphical view] |
| PROSITE | PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. PS50271. ZF_UBP. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 20872996. |
Entry information
| Entry name | UBP16_BOVIN | ||||||||
| Accession | Primary (citable) accession number: Q08DA3 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |