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Q08DA3

- UBP16_BOVIN

UniProt

Q08DA3 - UBP16_BOVIN

Protein

Ubiquitin carboxyl-terminal hydrolase 16

Gene

USP16

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 2 (11 Dec 2013)
      Previous versions | rss
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    Functioni

    Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.UniRule annotation

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi24 – 241Zinc 1UniRule annotation
    Metal bindingi26 – 261Zinc 1UniRule annotation
    Metal bindingi48 – 481Zinc 2UniRule annotation
    Metal bindingi51 – 511Zinc 2UniRule annotation
    Metal bindingi74 – 741Zinc 3UniRule annotation
    Metal bindingi77 – 771Zinc 3UniRule annotation
    Metal bindingi82 – 821Zinc 2UniRule annotation
    Metal bindingi90 – 901Zinc 2UniRule annotation
    Metal bindingi94 – 941Zinc 3UniRule annotation
    Metal bindingi103 – 1031Zinc 3UniRule annotation
    Metal bindingi116 – 1161Zinc 1UniRule annotation
    Metal bindingi119 – 1191Zinc 1UniRule annotation
    Active sitei204 – 2041NucleophileUniRule annotation
    Active sitei760 – 7601Proton acceptorUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri60 – 12566UBP-typeUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: UniProtKB
    2. histone binding Source: UniProtKB
    3. transcription coactivator activity Source: UniProtKB
    4. ubiquitin binding Source: UniProtKB
    5. ubiquitin-specific protease activity Source: UniProtKB
    6. ubiquitin thiolesterase activity Source: UniProtKB
    7. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. histone deubiquitination Source: UniProtKB
    2. mitotic nuclear division Source: UniProtKB
    3. monoubiquitinated histone H2A deubiquitination Source: UniProtKB
    4. positive regulation of transcription, DNA-templated Source: UniProtKB
    5. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    6. protein homotetramerization Source: UniProtKB
    7. regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    8. transcription, DNA-templated Source: UniProtKB-KW
    9. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Activator, Chromatin regulator, Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC19.021.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 16UniRule annotation (EC:3.4.19.12UniRule annotation)
    Alternative name(s):
    Deubiquitinating enzyme 16UniRule annotation
    Ubiquitin thioesterase 16UniRule annotation
    Ubiquitin-specific-processing protease 16UniRule annotation
    Gene namesi
    Name:USP16UniRule annotation
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 1

    Subcellular locationi

    Nucleus UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 826826Ubiquitin carboxyl-terminal hydrolase 16PRO_0000367501Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei414 – 4141PhosphoserineBy similarity
    Modified residuei551 – 5511PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity.UniRule annotation

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ08DA3.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000026801.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini195 – 825631USPAdd
    BLAST

    Domaini

    The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin.UniRule annotation

    Sequence similaritiesi

    Belongs to the peptidase C19 family. USP16 subfamily.UniRule annotation
    Contains 1 UBP-type zinc finger.UniRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri60 – 12566UBP-typeUniRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5207.
    GeneTreeiENSGT00750000117419.
    HOGENOMiHOG000154755.
    HOVERGENiHBG062704.
    InParanoidiQ08DA3.
    KOiK11844.
    OMAiAFNTYEC.
    TreeFamiTF326075.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    HAMAPiMF_03062. UBP16.
    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q08DA3-1 [UniParc]FASTAAdd to Basket

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    MGKKRTKGKT VPIDDSSESL EPVCRHIRKG LEQGNLKKAL VNVEWNICQD    50
    CKTDNKVKDK SEEETEENPS VWLCLKCGHQ GCGRNSQEQH ALKHYMKPRS 100
    EPHCLVLSLD NWSVWCYLCD DEVHYCNSNR LGQVVDYVRK QAGNTTPESA 150
    EDNGNIELEN KKLEKESKNE QEREKKENMA RENPSMNSTS QITVKGLSNL 200
    GNTCFFNAVM QNLSQTPVLR ELLKEVKMSG TIVKIEPPDL ALTEPLEINL 250
    EPPGPLTLAM SQFLNEMQET KKGIVTPREL FSQVCKKAVR FKGYQQQDSQ 300
    ELLRYLLDGM RAEEHQRVSK GILKAFDNST EKVDEELKNK VKEYEKKKSV 350
    PSFVDRIFGG ELTSTIMCDE CRTVSLVHES FLDLSLPVLD DQSGKKSIND 400
    KNLKKTMEDE DKDSEEEKDN DSYLKERNDI PSGTSKHLQK KAKKQAKKQA 450
    KNQRRQQKIQ GKVLHLNDVY AIDHPEDNEC EVEMSLQREA DIKSNHISQE 500
    EVAPKEYCVN QKDLNGHEKM IESITDNQKS TEEAAMKNVS VDNDLEVLAS 550
    SATECPRNLN GAYLKEGSNG EVDISSGFEN LNLNAALQPD EINIEILDDD 600
    PTPGTKVYEV VNEDPETAFC TLANREAFNT DECSVQHCLY QFTRNEKLRD 650
    ANKLLCEVCT RRQYSGPKAN MKGERKHIYT NAKKQMLISL APPVLTLHFK 700
    RFQQAGFNLR KVNKHIKFPE ILDLAPFCTL KCKNVAEEHT RVLYSLYGVV 750
    EHSGTMRSGH YTAYAKTRTA STHLSNLVLH GDIPQDFEME STKGQWFHIS 800
    DTHVQAVPTT KVLSSQAYLL FYERIL 826
    Length:826
    Mass (Da):94,050
    Last modified:December 11, 2013 - v2
    Checksum:i54A141CB98B711CE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti771 – 7711S → N in AAI23862. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DAAA02000172 Genomic DNA. No translation available.
    DAAA02000173 Genomic DNA. No translation available.
    BC123861 mRNA. Translation: AAI23862.1.
    RefSeqiNP_001070335.1. NM_001076867.1.
    UniGeneiBt.51616.

    Genome annotation databases

    EnsembliENSBTAT00000026801; ENSBTAP00000026801; ENSBTAG00000020122.
    GeneIDi519992.
    KEGGibta:519992.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DAAA02000172 Genomic DNA. No translation available.
    DAAA02000173 Genomic DNA. No translation available.
    BC123861 mRNA. Translation: AAI23862.1 .
    RefSeqi NP_001070335.1. NM_001076867.1.
    UniGenei Bt.51616.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000026801.

    Protein family/group databases

    MEROPSi C19.021.

    Proteomic databases

    PRIDEi Q08DA3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000026801 ; ENSBTAP00000026801 ; ENSBTAG00000020122 .
    GeneIDi 519992.
    KEGGi bta:519992.

    Organism-specific databases

    CTDi 10600.

    Phylogenomic databases

    eggNOGi COG5207.
    GeneTreei ENSGT00750000117419.
    HOGENOMi HOG000154755.
    HOVERGENi HBG062704.
    InParanoidi Q08DA3.
    KOi K11844.
    OMAi AFNTYEC.
    TreeFami TF326075.

    Miscellaneous databases

    NextBioi 20872996.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    HAMAPi MF_03062. UBP16.
    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Hereford.
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal muscle.

    Entry informationi

    Entry nameiUBP16_BOVIN
    AccessioniPrimary (citable) accession number: Q08DA3
    Secondary accession number(s): F1N2B1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: December 11, 2013
    Last modified: October 1, 2014
    This is version 60 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3