Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ubiquitin carboxyl-terminal hydrolase 16

Gene

USP16

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.UniRule annotation

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi24 – 241Zinc 1UniRule annotation
Metal bindingi26 – 261Zinc 1UniRule annotation
Metal bindingi48 – 481Zinc 2UniRule annotation
Metal bindingi51 – 511Zinc 2UniRule annotation
Metal bindingi74 – 741Zinc 3UniRule annotation
Metal bindingi77 – 771Zinc 3UniRule annotation
Metal bindingi82 – 821Zinc 2UniRule annotation
Metal bindingi90 – 901Zinc 2UniRule annotation
Metal bindingi94 – 941Zinc 3UniRule annotation
Metal bindingi103 – 1031Zinc 3UniRule annotation
Metal bindingi116 – 1161Zinc 1UniRule annotation
Metal bindingi119 – 1191Zinc 1UniRule annotation
Active sitei204 – 2041NucleophileUniRule annotation
Active sitei760 – 7601Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri60 – 12566UBP-typeUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: UniProtKB
  2. histone binding Source: UniProtKB
  3. transcription coactivator activity Source: UniProtKB
  4. ubiquitin binding Source: UniProtKB
  5. ubiquitin-specific protease activity Source: UniProtKB
  6. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. cellular response to DNA damage stimulus Source: Ensembl
  3. histone deubiquitination Source: UniProtKB
  4. histone H2A K63-linked deubiquitination Source: Ensembl
  5. mitotic nuclear division Source: UniProtKB
  6. monoubiquitinated histone H2A deubiquitination Source: UniProtKB
  7. positive regulation of transcription, DNA-templated Source: UniProtKB
  8. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  9. positive regulation of translational elongation Source: Ensembl
  10. protein homotetramerization Source: UniProtKB
  11. regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  12. transcription, DNA-templated Source: UniProtKB-KW
  13. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.021.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 16UniRule annotation (EC:3.4.19.12UniRule annotation)
Alternative name(s):
Deubiquitinating enzyme 16UniRule annotation
Ubiquitin thioesterase 16UniRule annotation
Ubiquitin-specific-processing protease 16UniRule annotation
Gene namesi
Name:USP16UniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 1

Subcellular locationi

  1. Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 826826Ubiquitin carboxyl-terminal hydrolase 16PRO_0000367501Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei414 – 4141PhosphoserineBy similarity
Modified residuei551 – 5511PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ08DA3.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026801.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini195 – 825631USPAdd
BLAST

Domaini

The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin.UniRule annotation

Sequence similaritiesi

Belongs to the peptidase C19 family. USP16 subfamily.UniRule annotation
Contains 1 UBP-type zinc finger.UniRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri60 – 12566UBP-typeUniRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5207.
GeneTreeiENSGT00780000121904.
HOGENOMiHOG000154755.
HOVERGENiHBG062704.
InParanoidiQ08DA3.
KOiK11844.
OMAiCATEESE.
OrthoDBiEOG7J9VNZ.
TreeFamiTF326075.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
HAMAPiMF_03062. UBP16.
InterProiIPR001394. Peptidase_C19_UCH.
IPR030849. UBP16.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q08DA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKRTKGKT VPIDDSSESL EPVCRHIRKG LEQGNLKKAL VNVEWNICQD
60 70 80 90 100
CKTDNKVKDK SEEETEENPS VWLCLKCGHQ GCGRNSQEQH ALKHYMKPRS
110 120 130 140 150
EPHCLVLSLD NWSVWCYLCD DEVHYCNSNR LGQVVDYVRK QAGNTTPESA
160 170 180 190 200
EDNGNIELEN KKLEKESKNE QEREKKENMA RENPSMNSTS QITVKGLSNL
210 220 230 240 250
GNTCFFNAVM QNLSQTPVLR ELLKEVKMSG TIVKIEPPDL ALTEPLEINL
260 270 280 290 300
EPPGPLTLAM SQFLNEMQET KKGIVTPREL FSQVCKKAVR FKGYQQQDSQ
310 320 330 340 350
ELLRYLLDGM RAEEHQRVSK GILKAFDNST EKVDEELKNK VKEYEKKKSV
360 370 380 390 400
PSFVDRIFGG ELTSTIMCDE CRTVSLVHES FLDLSLPVLD DQSGKKSIND
410 420 430 440 450
KNLKKTMEDE DKDSEEEKDN DSYLKERNDI PSGTSKHLQK KAKKQAKKQA
460 470 480 490 500
KNQRRQQKIQ GKVLHLNDVY AIDHPEDNEC EVEMSLQREA DIKSNHISQE
510 520 530 540 550
EVAPKEYCVN QKDLNGHEKM IESITDNQKS TEEAAMKNVS VDNDLEVLAS
560 570 580 590 600
SATECPRNLN GAYLKEGSNG EVDISSGFEN LNLNAALQPD EINIEILDDD
610 620 630 640 650
PTPGTKVYEV VNEDPETAFC TLANREAFNT DECSVQHCLY QFTRNEKLRD
660 670 680 690 700
ANKLLCEVCT RRQYSGPKAN MKGERKHIYT NAKKQMLISL APPVLTLHFK
710 720 730 740 750
RFQQAGFNLR KVNKHIKFPE ILDLAPFCTL KCKNVAEEHT RVLYSLYGVV
760 770 780 790 800
EHSGTMRSGH YTAYAKTRTA STHLSNLVLH GDIPQDFEME STKGQWFHIS
810 820
DTHVQAVPTT KVLSSQAYLL FYERIL
Length:826
Mass (Da):94,050
Last modified:December 11, 2013 - v2
Checksum:i54A141CB98B711CE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti771 – 7711S → N in AAI23862 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DAAA02000172 Genomic DNA. No translation available.
DAAA02000173 Genomic DNA. No translation available.
BC123861 mRNA. Translation: AAI23862.1.
RefSeqiNP_001070335.1. NM_001076867.1.
UniGeneiBt.51616.

Genome annotation databases

EnsembliENSBTAT00000026801; ENSBTAP00000026801; ENSBTAG00000020122.
GeneIDi519992.
KEGGibta:519992.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DAAA02000172 Genomic DNA. No translation available.
DAAA02000173 Genomic DNA. No translation available.
BC123861 mRNA. Translation: AAI23862.1.
RefSeqiNP_001070335.1. NM_001076867.1.
UniGeneiBt.51616.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026801.

Protein family/group databases

MEROPSiC19.021.

Proteomic databases

PRIDEiQ08DA3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000026801; ENSBTAP00000026801; ENSBTAG00000020122.
GeneIDi519992.
KEGGibta:519992.

Organism-specific databases

CTDi10600.

Phylogenomic databases

eggNOGiCOG5207.
GeneTreeiENSGT00780000121904.
HOGENOMiHOG000154755.
HOVERGENiHBG062704.
InParanoidiQ08DA3.
KOiK11844.
OMAiCATEESE.
OrthoDBiEOG7J9VNZ.
TreeFamiTF326075.

Miscellaneous databases

NextBioi20872996.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
HAMAPiMF_03062. UBP16.
InterProiIPR001394. Peptidase_C19_UCH.
IPR030849. UBP16.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hereford.
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal muscle.

Entry informationi

Entry nameiUBP16_BOVIN
AccessioniPrimary (citable) accession number: Q08DA3
Secondary accession number(s): F1N2B1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 11, 2013
Last modified: April 29, 2015
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.