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Protein

Ubiquitin carboxyl-terminal hydrolase 16

Gene

USP16

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.UniRule annotation

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi24Zinc 1UniRule annotation1
Metal bindingi26Zinc 1UniRule annotation1
Metal bindingi48Zinc 2UniRule annotation1
Metal bindingi51Zinc 2UniRule annotation1
Metal bindingi74Zinc 3UniRule annotation1
Metal bindingi77Zinc 3UniRule annotation1
Metal bindingi82Zinc 2UniRule annotation1
Metal bindingi90Zinc 2UniRule annotation1
Metal bindingi94Zinc 3UniRule annotation1
Metal bindingi103Zinc 3UniRule annotation1
Metal bindingi116Zinc 1UniRule annotation1
Metal bindingi119Zinc 1UniRule annotation1
Active sitei204NucleophileUniRule annotation1
Active sitei760Proton acceptorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri60 – 125UBP-typeUniRule annotationAdd BLAST66

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, Chromatin regulator, Hydrolase, Protease, Thiol protease
Biological processCell cycle, Cell division, Mitosis, Transcription, Transcription regulation, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-BTA-5689880. Ub-specific processing proteases.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 16UniRule annotation (EC:3.4.19.12UniRule annotation)
Alternative name(s):
Deubiquitinating enzyme 16UniRule annotation
Ubiquitin thioesterase 16UniRule annotation
Ubiquitin-specific-processing protease 16UniRule annotation
Gene namesi
Name:USP16UniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 1

Subcellular locationi

  • Nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003675011 – 826Ubiquitin carboxyl-terminal hydrolase 16Add BLAST826

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki140Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei188PhosphoserineBy similarity1
Modified residuei414PhosphoserineBy similarity1
Modified residuei530PhosphoserineBy similarity1
Modified residuei551PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity.UniRule annotation

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ08DA3.
PRIDEiQ08DA3.

Expressioni

Gene expression databases

BgeeiENSBTAG00000020122.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026801.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini195 – 825USPAdd BLAST631

Domaini

The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin.UniRule annotation

Sequence similaritiesi

Belongs to the peptidase C19 family. USP16 subfamily.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri60 – 125UBP-typeUniRule annotationAdd BLAST66

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1873. Eukaryota.
COG5560. LUCA.
GeneTreeiENSGT00670000097750.
HOGENOMiHOG000154755.
HOVERGENiHBG062704.
InParanoidiQ08DA3.
KOiK11844.
OMAiFHISDTS.
OrthoDBiEOG091G01WT.
TreeFamiTF326075.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
HAMAPiMF_03062. UBP16. 1 hit.
InterProiView protein in InterPro
IPR001394. Peptidase_C19_UCH.
IPR030849. UBP16.
IPR018200. USP_CS.
IPR028889. USP_dom.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
PfamiView protein in Pfam
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
SMARTiView protein in SMART
SM00290. ZnF_UBP. 1 hit.
PROSITEiView protein in PROSITE
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q08DA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKKRTKGKT VPIDDSSESL EPVCRHIRKG LEQGNLKKAL VNVEWNICQD
60 70 80 90 100
CKTDNKVKDK SEEETEENPS VWLCLKCGHQ GCGRNSQEQH ALKHYMKPRS
110 120 130 140 150
EPHCLVLSLD NWSVWCYLCD DEVHYCNSNR LGQVVDYVRK QAGNTTPESA
160 170 180 190 200
EDNGNIELEN KKLEKESKNE QEREKKENMA RENPSMNSTS QITVKGLSNL
210 220 230 240 250
GNTCFFNAVM QNLSQTPVLR ELLKEVKMSG TIVKIEPPDL ALTEPLEINL
260 270 280 290 300
EPPGPLTLAM SQFLNEMQET KKGIVTPREL FSQVCKKAVR FKGYQQQDSQ
310 320 330 340 350
ELLRYLLDGM RAEEHQRVSK GILKAFDNST EKVDEELKNK VKEYEKKKSV
360 370 380 390 400
PSFVDRIFGG ELTSTIMCDE CRTVSLVHES FLDLSLPVLD DQSGKKSIND
410 420 430 440 450
KNLKKTMEDE DKDSEEEKDN DSYLKERNDI PSGTSKHLQK KAKKQAKKQA
460 470 480 490 500
KNQRRQQKIQ GKVLHLNDVY AIDHPEDNEC EVEMSLQREA DIKSNHISQE
510 520 530 540 550
EVAPKEYCVN QKDLNGHEKM IESITDNQKS TEEAAMKNVS VDNDLEVLAS
560 570 580 590 600
SATECPRNLN GAYLKEGSNG EVDISSGFEN LNLNAALQPD EINIEILDDD
610 620 630 640 650
PTPGTKVYEV VNEDPETAFC TLANREAFNT DECSVQHCLY QFTRNEKLRD
660 670 680 690 700
ANKLLCEVCT RRQYSGPKAN MKGERKHIYT NAKKQMLISL APPVLTLHFK
710 720 730 740 750
RFQQAGFNLR KVNKHIKFPE ILDLAPFCTL KCKNVAEEHT RVLYSLYGVV
760 770 780 790 800
EHSGTMRSGH YTAYAKTRTA STHLSNLVLH GDIPQDFEME STKGQWFHIS
810 820
DTHVQAVPTT KVLSSQAYLL FYERIL
Length:826
Mass (Da):94,050
Last modified:December 11, 2013 - v2
Checksum:i54A141CB98B711CE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti771S → N in AAI23862 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DAAA02000172 Genomic DNA. No translation available.
DAAA02000173 Genomic DNA. No translation available.
BC123861 mRNA. Translation: AAI23862.1.
RefSeqiNP_001070335.1. NM_001076867.1.
UniGeneiBt.51616.

Genome annotation databases

EnsembliENSBTAT00000026801; ENSBTAP00000026801; ENSBTAG00000020122.
GeneIDi519992.
KEGGibta:519992.

Similar proteinsi

Entry informationi

Entry nameiUBP16_BOVIN
AccessioniPrimary (citable) accession number: Q08DA3
Secondary accession number(s): F1N2B1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: December 11, 2013
Last modified: August 30, 2017
This is version 86 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families