ID   GLPK5_BOVIN             Reviewed;         528 AA.
AC   Q08D86; Q58DK9;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   16-JUN-2009, entry version 20.
DE   RecName: Full=Putative glycerol kinase 5;
DE            EC=2.7.1.30;
DE   AltName: Full=ATP:glycerol 3-phosphotransferase;
GN   Name=GK5;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX   PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences.";
RL   BMC Genomics 6:166-166(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=Hereford; TISSUE=Fetal skin;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: ATP + glycerol = ADP + sn-glycerol 3-
CC       phosphate.
CC   -!- PATHWAY: Polyol metabolism; glycerol degradation via glycerol
CC       kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q08D86-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q08D86-2; Sequence=VSP_032119, VSP_032120;
CC   -!- SIMILARITY: Belongs to the FGGY kinase family.
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DR   EMBL; BT021588; AAX46435.1; -; mRNA.
DR   EMBL; BC123888; AAI23889.1; -; mRNA.
DR   IPI; IPI00711070; -.
DR   IPI; IPI00807226; -.
DR   RefSeq; NP_001030550.2; -.
DR   UniGene; Bt.91577; -.
DR   Ensembl; ENSBTAG00000018138; Bos taurus.
DR   GeneID; 616031; -.
DR   KEGG; bta:616031; -.
DR   HOVERGEN; Q08D86; -.
DR   BRENDA; 2.7.1.30; 251.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004370; F:glycerol kinase activity; IEA:EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006072; P:glycerol-3-phosphate metabolic process; IEA:InterPro.
DR   InterPro; IPR000577; Carb_kinase_FGGY.
DR   InterPro; IPR018485; Carb_kinase_FGGY_C.
DR   InterPro; IPR018483; Carb_kinase_FGGY_CS.
DR   InterPro; IPR018484; Carb_kinase_FGGY_N.
DR   InterPro; IPR005999; Glycerol_kin.
DR   PANTHER; PTHR10196; FGGY_kin; 1.
DR   PANTHER; PTHR10196:SF9; Glycerol_kin; 1.
DR   Pfam; PF02782; FGGY_C; 1.
DR   Pfam; PF00370; FGGY_N; 1.
DR   PROSITE; PS00933; FGGY_KINASES_1; FALSE_NEG.
DR   PROSITE; PS00445; FGGY_KINASES_2; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; ATP-binding; Glycerol metabolism; Kinase;
KW   Nucleotide-binding; Transferase.
FT   CHAIN         1    528       Putative glycerol kinase 5.
FT                                /FTId=PRO_0000323753.
FT   NP_BIND     439    444       ATP (By similarity).
FT   BINDING      28     28       Substrate (By similarity).
FT   BINDING      98     98       Substrate (By similarity).
FT   BINDING     275    275       Substrate (By similarity).
FT   BINDING     297    297       ATP (By similarity).
FT   VAR_SEQ     350    409       DLFTDAAETEKMAQSLEDSEGVYFVPSFSGLQAPLNDPCAC
FT                                ASFMGLKPSTNKYHLVRAI -> GRMKKHSKNKNKGIISPL
FT                                SVL (in isoform 2).
FT                                /FTId=VSP_032119.
FT   VAR_SEQ     410    528       Missing (in isoform 2).
FT                                /FTId=VSP_032120.
SQ   SEQUENCE   528 AA;  58765 MW;  4616C70AE1ECF649 CRC64;
     MSEQSTVPEQ SASDSRRAGF VLGVDVGSSM IRCHVYDRAA RICGSSAQKV ESLYPQPGWV
     EIDPDVLWLQ FVAVIKESVK AAGIETNQIV GLGISTQRAT FITWDKKTGK HFHNFISWQD
     LRATELVKSW NGSLLMKLIH SSCRVLHFFT RSKQFLAASL FTFTTQHVSL RLAWILQNLT
     EVQKAVEEEN CCFGTVDTWL LHKLTKGSEF ATDFSNASTT GLFDPYEMCW SKIVTSLLSI
     PLSLLPPVKD TSHNFGSVDE EIFGVPIPIV ALVADQQAAM FGECCFQTGD VKLTMGTGTF
     LDINTGNNPQ QSVGGFYPLI GWKIGQEVVC LAESNAGDTG TAIEWAQQLD LFTDAAETEK
     MAQSLEDSEG VYFVPSFSGL QAPLNDPCAC ASFMGLKPST NKYHLVRAIL ESIAFRNKQL
     YETMQKEIHI PVRKIRADGG VCKNSFVMQM TSDLINETID RPVHIDMSCS GAASLAGLAV
     GFWSDKEELK KLRQSEMVFK PQKKWQEYEV NMGNWVKAVK RSMNWYKT
//