Reviewed, UniProtKB/Swiss-Prot Q08D86 (GLPK5_BOVIN)

Names and origin

Protein names

Recommended name:
    Putative glycerol kinase 5
      Short name=Glycerokinase 5
      Short name=GK 5
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase 5

Gene names

Name:

GK5

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	528 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate.
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q08D86-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q08D86-2)

The sequence of this isoform differs from the canonical sequence as follows:
     350-409: DLFTDAAETE...TNKYHLVRAI → GRMKKHSKNKNKGIISPLSVL
     410-528: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 528	528	Putative glycerol kinase 5		PRO_0000323753
Regions
	Nucleotide binding	439 – 444	6	ATP By similarity
Sites
	Binding site	28	1	Substrate By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	275	1	Substrate By similarity
	Binding site	297	1	ATP By similarity
Natural variations
	Alternative sequence	350 – 409	60	DLFTD…LVRAI → GRMKKHSKNKNKGIISPLSV L in isoform 2.		VSP_032119
	Alternative sequence	410 – 528	119	Missing in isoform 2.		VSP_032120

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 31, 2006. Version 1. Checksum: 4616C70AE1ECF649	FASTA	528	58,765
	10 20 30 40 50 60 MSEQSTVPEQ SASDSRRAGF VLGVDVGSSM IRCHVYDRAA RICGSSAQKV ESLYPQPGWV 70 80 90 100 110 120 EIDPDVLWLQ FVAVIKESVK AAGIETNQIV GLGISTQRAT FITWDKKTGK HFHNFISWQD 130 140 150 160 170 180 LRATELVKSW NGSLLMKLIH SSCRVLHFFT RSKQFLAASL FTFTTQHVSL RLAWILQNLT 190 200 210 220 230 240 EVQKAVEEEN CCFGTVDTWL LHKLTKGSEF ATDFSNASTT GLFDPYEMCW SKIVTSLLSI 250 260 270 280 290 300 PLSLLPPVKD TSHNFGSVDE EIFGVPIPIV ALVADQQAAM FGECCFQTGD VKLTMGTGTF 310 320 330 340 350 360 LDINTGNNPQ QSVGGFYPLI GWKIGQEVVC LAESNAGDTG TAIEWAQQLD LFTDAAETEK 370 380 390 400 410 420 MAQSLEDSEG VYFVPSFSGL QAPLNDPCAC ASFMGLKPST NKYHLVRAIL ESIAFRNKQL 430 440 450 460 470 480 YETMQKEIHI PVRKIRADGG VCKNSFVMQM TSDLINETID RPVHIDMSCS GAASLAGLAV 490 500 510 520 GFWSDKEELK KLRQSEMVFK PQKKWQEYEV NMGNWVKAVK RSMNWYKT
	Isoform 2. Checksum: 156D544A0119BD98	FASTA	370	40,776
	10 20 30 40 50 60 MSEQSTVPEQ SASDSRRAGF VLGVDVGSSM IRCHVYDRAA RICGSSAQKV ESLYPQPGWV 70 80 90 100 110 120 EIDPDVLWLQ FVAVIKESVK AAGIETNQIV GLGISTQRAT FITWDKKTGK HFHNFISWQD 130 140 150 160 170 180 LRATELVKSW NGSLLMKLIH SSCRVLHFFT RSKQFLAASL FTFTTQHVSL RLAWILQNLT 190 200 210 220 230 240 EVQKAVEEEN CCFGTVDTWL LHKLTKGSEF ATDFSNASTT GLFDPYEMCW SKIVTSLLSI 250 260 270 280 290 300 PLSLLPPVKD TSHNFGSVDE EIFGVPIPIV ALVADQQAAM FGECCFQTGD VKLTMGTGTF 310 320 330 340 350 360 LDINTGNNPQ QSVGGFYPLI GWKIGQEVVC LAESNAGDTG TAIEWAQQLG RMKKHSKNKN 370 KGIISPLSVL

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: Hereford. Tissue: Fetal skin.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT021588 mRNA. Translation: AAX46435.1. BC123888 mRNA. Translation: AAI23889.1.
IPI	IPI00711070. IPI00807226.
RefSeq	NP_001030550.2.
UniGene	Bt.91577
3D structure databases
SMR	Q08D86. Positions 20-528.
ModBase	Search...
Protein-protein interaction databases
STRING	Q08D86.
Genome annotation databases
Ensembl	ENSBTAT00000024143; ENSBTAP00000024143; ENSBTAG00000018138; Bos taurus. [Genome view] ENSBTAT00000034692; ENSBTAP00000034578; ENSBTAG00000018138; Bos taurus. [Genome view]
GeneID	616031.
KEGG	bta:616031.
Organism-specific databases
CTD	616031.
Phylogenomic databases
eggNOG	maNOG08695.
HOVERGEN	Q08D86.
InParanoid	Q08D86.
Enzyme and pathway databases
BRENDA	2.7.1.30. 251.
Family and domain databases
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
PROSITE	PS00933. FGGY_KINASES_1. False negative. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK5_BOVIN

Accession

Primary (citable) accession number: Q08D86
Secondary accession number(s): Q58DK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	October 31, 2006
Last modified:	January 19, 2010
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)